Myanmar traditional medicine formulations and their antioxidant, antiglycation and alpha-glucosidase inhibitory activities: Potentials for antidiabetes complications

Myanmar Traditional Medicine (MTM) has been used since ancient times to treat life-threatening diseases like diabetes. In this study, various in vitro assays were used to prove that four MTM formulations were effective at treating diabetes. Antioxidant activities were determined using in vitro DPPH, nitric oxide (NO), and superoxide (SO) radical scavenging assays. The Folin-Ciocalteu method was used to quantify the total phenolic content, while the BSA-fluorescent antiglycation and α-glucosidase inhibitory assays were utilized to determine the antidiabetic activity of MTMs. Among the tested samples, MTM3 showed the best activities for almost all the biological assays tested in this experiment with the % inhibition of 82.89 ± 1.64 for NO and 65.02 ± 2.82 for SO radical scavenging activity, 92.12 ± 1.18 for α-glucosidase inhibitory activity and IC50 of 180.29 ± 1.6 µg/ml for the antiglycation activity. It also possessed the highest total phenolic content of 149.41 ± 3.7 mg GAE/g of extract/l among the tested samples. Therefore, the findings suggested that MTM could help diabetic patients improve their quality of life through antioxidant activity against several free radicals and their antiglycation and α-glucosidase inhibitory characteristics

In vitro antidiabetic activities of Myanmar medicinal plants: Cassia siamea Lam. and Butea monosperma Roxb.

This study aimed to evaluate the antidiabetic potentials of Cassia siamea and Butea monosperma. Cytotoxic activity of test extracts was performed by a hemolytic assay. Estimation of the antidiabetic properties was explored by α-glucosidase and DPP-IV inhibition assays. The glucose transportation activity of test extracts across the yeast cells was expressed by a glucose uptake assay. Non-hemolytic effects of test extracts were shown as lysis per cent less than 15 on RBCs. The inhibition potential of test extracts on α-glucosidase enzyme illustrates that IC50 values (μg/mL) of C. siamea was 76.33±12.2 and B. monosperma was 77.28±2.02. While the IC50 values of the acarbose was 36.76±1.55 μg/mL. In addition, the tested extracts showed the ability to inhibit DPP-IV enzyme activity in a concentration-dependent manner. The IC50 (μg/mL) values of C. siamea, B. monosperma, and the sitagliptin were 117.02±9.73, 103±8.5, and 144.85±13.43, respectively in DPP-IV inhibitory assay. Moreover, the test extracts could transport glucose in yeast cells representing the glucose uptake effectively especially in B. monosperma with a concentration-dependent manner in all tested glucose concentrations. This study provided that the tested extracts promise to possess the antidiabetic potential with non-hemolytic properties, diabetic-enzymes suppressing potency and glucose utilizing ability

Time to stop polishing the brass on the Titanic: moving beyond ‘quick-and-dirty’ teacher education for inclusion, towards sustainable theories of change

Interest in inclusive education in the global south has grown significantly since the adoption of the Salamanca Statement in 1994. Increasingly, those who fund and provide education want to be seen taking action on inclusion generally and disability inclusion specifically. However, the much-welcomed enthusiasm to respond to global commitments is not always matched with the necessary expertise and commitment to longer-term action and change. The growth in inclusive education policies and pilot projects in the last decade is hard to miss, but changes resulting from these interventions are often less apparent. Why is that? Drawing on the Enabling Education Network’s 22 years of experience as a global inclusive education network and consultancy provider, we present alternative pathways for change in teacher education for inclusion. We stress that change in teaching practice remains limited not because inclusive education is a fundamentally flawed concept, but because too much focus is given to ‘quick-and-dirty’ trainings that quickly yield donorpleasing statistics and publicity-attracting case studies, but fail to elicit sufficiently extensive and sustainable change to education systems and cultures

In vitro antidiabetic activities of Myanmar medicinal plants: Cassia siamea Lam. and Butea monosperma Roxb.

483-490This study aimed to evaluate the antidiabetic potentials of Cassia siamea and Butea monosperma. Cytotoxic activity of test extracts was performed by a hemolytic assay. Estimation of the antidiabetic properties was explored by α-glucosidase and DPP-IV inhibition assays. The glucose transportation activity of test extracts across the yeast cells was expressed by a glucose uptake assay. Non-hemolytic effects of test extracts were shown as lysis per cent less than 15 on RBCs. The inhibition potential of test extracts on α-glucosidase enzyme illustrates that IC50 values (μg/mL) of C. siamea was 76.33±12.2 and B. monosperma was 77.28±2.02. While the IC50 values of the acarbose was 36.76±1.55 μg/mL. In addition, the tested extracts showed the ability to inhibit DPP-IV enzyme activity in a concentration-dependent manner. The IC50 (μg/mL) values of C. siamea, B. monosperma, and the sitagliptin were 117.02±9.73, 103±8.5, and 144.85±13.43, respectively in DPP-IV inhibitory assay. Moreover, the test extracts could transport glucose in yeast cells representing the glucose uptake effectively especially in B. monosperma with a concentration-dependent manner in all tested glucose concentrations. This study provided that the tested extracts promise to possess the antidiabetic potential with non-hemolytic properties, diabetic-enzymes suppressing potency and glucose utilizing ability

The complete chloroplast genome sequence of Lilium speciosum Var. gloriosoides, an important breeding parent

Lilium speciosum var. gloriosoides is an important breeding parent with high ornamental and edible value in worldwide. In this study, we reported a complete chloroplast genome of L. speciosum var. gloriosoides, which was de novo assembled using the next-generation sequencing data. The whole genome is 152,912 bp in length and includes one large single copy (LSC) region of 70,693 bp, one small single copy (SSC) region of 17,517 bp, and a pair of inverted repeat (IR) region of 26,539 bp. A total of 131 functional genes were encoded, consisting of 76 protein-coding genes, 36 transfer RNA genes, and eight ribosomal RNA genes. The overall AT content of the chloroplast genome is 63.00%. In the maximum likelihood, a strong phylogenetic signal showed that L. speciosum var. gloriosoides is a species of Lilium, which is the first major genome in section Archelirion

Isolation and Characterization of Plant Growth-Promoting Endophytic Bacteria Paenibacillus polymyxa SK1 from Lilium lancifolium

Paenibacillus polymyxa is a plant growth-promoting rhizobacterium that has immense potential to be used as an environmentally friendly replacement of chemical fertilizers and pesticides. In the present study, Paenibacillus polymyxa SK1 was isolated from bulbs of Lilium lancifolium. The isolated endophytic strain showed antifungal activities against important plant pathogens like Botryosphaeria dothidea, Fusarium oxysporum, Botrytis cinerea, and Fusarium fujikuroi. The highest percentage of growth inhibition, i.e., 66.67 ± 2.23%, was observed for SK1 against Botryosphaeria dothidea followed by 61.19 ± 3.12%, 60.71 ± 3.53%, and 55.54 ± 2.89% against Botrytis cinerea, Fusarium fujikuroi, and Fusarium oxysporum, respectively. The metabolite profiling of ethyl acetate fraction was assessed through the UHPLC-LTQ-IT-MS/MS analysis, and putative identification was done with the aid of the GNPS molecular networking workflow. A total of 29 compounds were putatively identified which included dipeptides, tripeptides, cyclopeptides (cyclo-(Leu-Leu), cyclo(Pro-Phe)), 2-heptyl-3-hydroxy 4-quinolone, 6-oxocativic acid, anhydrobrazilic acid, 1-(5-methoxy-1H-indol-3-yl)-2-piperidin-1-ylethane-1,2-dione, octadecenoic acid, pyochelin, 15-hydroxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid, (Z)-7-[(2R,3S)-3-[(2Z,5E)-Undeca-2,5-dienyl]oxiran-2-yl]hept-5-enoic acid, arginylasparagine, cholic acid, sphinganine, elaidic acid, gossypin, L-carnosine, tetrodotoxin, and ursodiol. The high antifungal activity of SK1 might be attributed to the presence of these bioactive compounds. The isolated strain SK1 showed plant growth-promoting traits such as the production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, nitrogen fixation, and phosphate solubilization. IAA production was strongly correlated with the application of exogenous tryptophan concentrations in the medium. Furthermore, inoculation of SK1 enhanced plant growth of two Lilium varieties, Tresor and White Heaven, under greenhouse condition. In the light of these findings, the P. polymyxa SK1 may be utilized as a source of plant growth promotion and disease control in sustainable agriculture