Modelos numéricos para la simulación de incendios en túneles con ventilación semi transversal y trampillas de extracción

El incremento experimentado por la construcción de grandes túneles ha provocado un renovado interés por el estudio de las diferentes situaciones de accidente que se pueden producir durante su vida de servicio. Entre ellos uno de los más complicados y peor conocidos es el caso de un accidente con fuego en el interior del túnel. Mediante el uso de un método numérico de volúmenes finitos este articulo presenta un estudio sobre las implicaciones del uso de trampillas de extracción de humos así como algunas conclusiones obtenidas tras el estudio de los resultados

SARS-CoV-2 viral RNA detection using the novel CoVradar device associated with the CoVreader smartphone app

Supplementary data to this article can be found online at https://doi. org/10.1016/j.bios.2023.115268The COVID-19 pandemic has highlighted the need for innovative approaches to its diagnosis. Here we present CoVradar, a novel and simple colorimetric method that combines nucleic acid analysis with dynamic chemical labeling (DCL) technology and the Spin-Tube device to detect SARS-CoV-2 RNA in saliva samples. The assay includes a fragmentation step to increase the number of RNA templates for analysis, using abasic peptide nucleic acid probes (DGL probes) immobilized to nylon membranes in a specific dot pattern to capture RNA fragments. Duplexes are formed by labeling complementary RNA fragments with biotinylated SMART bases, which act as templates for DCL. Signals are generated by recognizing biotin with streptavidin alkaline phosphatase and incubating with a chromogenic substrate to produce a blue precipitate. CoVradar results are analysed by CoVreader, a smartphone-based image processing system that can display and interpret the blotch pattern. CoVradar and CoVreader provide a unique molecular assay capable of detecting SARS-CoV-2 viral RNA without the need for extraction, preamplification, or pre-labeling steps, offering advantages in terms of time (similar to 3 h/test), cost (similar to epsilon 1/test manufacturing cost) and simplicity (does not require large equipment). This solution is also promising for developing assays for other infectious diseases.FEDER/Junta de Andalucia-Consejeria de Economia y Conocimiento CV20-77741, A-FQM-760-UGR20, PID 2019-110987RB-I00, PID 2019-103938RB-I00Spanish MCIN/AEI P18-RT-2961, P18-TP-4160FEDER/Junta de Andalucia-Consejeria de Salud y Familias PIP-0232-2021European CommissionMCIN/AEI PTQ 2020-011388, IJC 2020-043307-IEuropean Union NextGenerationEU/PRTR PTQ 2020-011388, IJC 2020-043307-

Smartphone-Based Diagnosis of Parasitic Infections With Colorimetric Assays in Centrifuge Tubes

A smartphone-based platform for the diagnosis of parasitic infections has been developed, tested and validated. The system is capable of making automatic and accurate analysis of millimetric colorimetric arrays in centrifuge collection tubes, which are well established tools used in clinical analysis. To that end, an Android-based software application has been developed, making use of the smartphone rear camera, enabling precise image processing of the colorimetric spot arrays. A low-cost plastic accessory has been developed using 3D-printing to provide controlled illumination, xed sample positioning and cell phone attachment. The platform was then tested repeatedly for its size detection, edge blurriness and colour detection capabilities. A minimum spot radius of 175 m is detectable when using the developed app, with a tolerance of 15%, corresponding to 0.25%of the area where the spot array is printed. Spot edge de nition has been studied up to 40% of blurriness, resulting in a low average percentage error of 1.24%. Colour detection follows the well-known Gamma correction function. Finally, the whole platform was tested and validated using real DNA to analyse for accurate discrimination of Trypanosomatid species, which are responsible for devastating diseases in humans and livestock. The smartphone-based platform can be further extended to other clinical analysis. Its simplicity and reliable performance mean it can be used in remote, limited-resource settings by relatively unskilled technicians/nurses, where diagnostic laboratories are sparsely distributed. The results can however be sent easily via the smartphone to medical experts as well as government health agencies.This work was supported in part by the Spanish Ministry of Economics and Competitivity under Project CTQ2016-78754-C2-1-R, in part by the European Regional Development Fund (ERDF), and in part by the DestiNA Genomica SL provided reagents, samples and Spin-Tube devices. The work of P. Escobedo was supported by the Spanish Ministry of Education, Culture and Sport (MECD), under Grant (FPU13/05032)

Influence of the chirality of short peptide supramolecular hydrogels in protein crystallogenesis

For the first time the influence of the chirality of the gel fibers in protein crystallogenesis has been studied. Enantiomeric hydrogels 1 and 2 were tested with model proteins lysozyme and glucose isomerase and a formamidase from B. cereus. Crystallization behaviour and crystal quality of these proteins in both hydrogels are presented and compared.MICINN (Spain) projects BIO2010-16800 (JAG), CTQ-2011.22455 (LAC & JMC), CTQ2012-34778 (JJDM & ALG), “Factoría Española de Cristalización” Consolider-Ingenio 2010 (JAG & MCM) and EDRF Funds (JAG, LAC & JMC), P12-FQM-2721 (LAC) Junta de Andalucía.MINECO,Project No. FIS2013-41821-R

Actos de gestión tributaria reclamables en el procedimiento económico-administrativo

Universidad de Granada, Departamento de Derecho Financiero y Tributario. Leída en 199

Haciendas locales y participación en tributos del Estado

Hacienda local, Financiación local, Tributos, Impuestos, Tasas

Catalytic and Electron Conducting Carbon Nanotube Reinforced Lysozyme Crystals

Novel reinforced cross-linked lysozyme crystals containing homogeneous dispersions of single-walled carbon nanotubes (SWCNTs) have been produced and characterized. The incorporation of SWCNTs inside lysozyme crystals gives rise to reinforced hybrid materials with tunable mechanical strength and electronic conductivity, while preserving the crystal-quality and morphology. These reinforced crystals show increase catalytic activity at higher temperature, being active even above the denaturation temperature. The electron transport through the crystals have been linked to the content and distribution of SWCNTs inside the crystals. The electron conduction through the crystals is isotropic and very efficient, presenting high conductivity values up to 600 nS at very low (0.05 wt%) SWCNTs concentration. To obtain these crystals a new protocol based on the in situ crystallization of lysozyme in hybrid SWCNTs-peptide hydrogels has been developed. These peptide hydrogels have been able to homogeneously dispersed hydrophobic SWCNTs allowing first, the crystallization of the enzyme lysozyme and secondly, transferring the new properties of the inorganic component to the crystals. Taken together, these hybrid crystals represent a notorious example of the versatility of proteins as biological substrates in the generation of novel functional materials, opening the door to use them in catalysis and bioelectronics at macroscale.Projects BIO2016-74875-P and FIS2017-85954-R (Ministerio de Economía, Industria y Competitividad, MINECO, and Agencia Estatal de Investigación, AEI, Spain, co-funded by Fondo Europeo de Desarrollo Regional, FEDER, European Union) and by Junta de Andalucía (Spain) projects P12-FQM-2721 and P12-FQM-790

Close city: neighborhoods and equipment. Workshop plans and projects, 2011-2012. Master of Urbanism, University of Granada

Esta publicación describe los trabajos del Taller de Proyectos y Planes del II semestre del Máster de Urbanismo, curso 2011-2012. En este Taller nos propusimos trabajar sobre la ciudad de Granada, a escala de barrio, poniendo el centro de atención en los espacios dedicados a dotaciones, comúnmente llamados equipamientos. Y ello, desde la convicción de que una ciudad, para ser confortable y merecer el aprecio de sus ciudadanos, necesita que éstos puedan disponer y acceder, de manera cómoda y sin exclusiones, a aquellos servicios que constituyen hoy un derecho inalienable para todos.This publication describes the work by the Projects and Plans II semester of the Master of Urban Planning, 2011-2012 academic year. In this workshop we set to work on the city of Granada, at neighborhood, putting the spotlight on the areas devoted to provisions, commonly called equipment. And this, from the conviction that a city, to be comfortable and deserve the appreciation of its citizens requires that they can arrange and access, comfortably and without exception, those services currently constitute an inalienable right for all