AINTEGUMENTA and the D-type cyclin CYCD3;1 regulate root secondary growth and respond to cytokinins

Higher plant vasculature is characterized by two distinct developmental phases. Initially, a well-defined radial primary pattern is established. In eudicots, this is followed by secondary growth, which involves development of the cambium and is required for efficient water and nutrient transport and wood formation. Regulation of secondary growth involves several phytohormones, and cytokinins have been implicated as key players, particularly in the activation of cell proliferation, but the molecular mechanisms mediating this hormonal control remain unknown. Here we show that the genes encoding the transcription factor AINTEGUMENTA (ANT) and the D-type cyclin CYCD3;1 are expressed in the vascular cambium of Arabidopsis roots, respond to cytokinins and are both required for proper root secondary thickening. Cytokinin regulation of ANT and CYCD3 also occurs during secondary thickening of poplar stems, suggesting this represents a conserved regulatory mechanism.Peer reviewe

Plant vascular development: from early specification to differentiation.

Vascular tissues in plants are crucial to provide physical support and to transport water, sugars and hormones and other small signalling molecules throughout the plant. Recent genetic and molecular studies have identified interconnections among some of the major signalling networks that regulate plant vascular development. Using Arabidopsis thaliana as a model system, these studies enable the description of vascular development from the earliest tissue specification events during embryogenesis to the differentiation of phloem and xylem tissues. Moreover, we propose a model for how oriented cell divisions give rise to a three-dimensional vascular bundle within the root meristem

Molecular Control of Cell Specification and Cell Differentiation During Procambial Development

Land plants develop vascular tissues that enable the long-distance transport of water and nutrients in xylem and phloem, provide mechanical support for their vertical growth, and produce cells in radial growth. Vascular tissues are produced in many parts of the plant and during different developmental stages. Early vascular development is focused in procambial meristems, and in some species it continues during the secondary phase of plant development in cambial meristems. In this review, we highlight recent progress in understanding procambial development. This involves the analysis of stem cell–like properties of procambial tissues, specification of xylem and phloem, and differentiation of the conductive tissues. Several major plant hormones, small-RNA species, and transcriptional networks play a role in vascular development. We describe current approaches to integrating these networks as well as their potential role in explaining the diversity and evolution of plant vascular systems

In Situ Localization of Small RNAs in Plants

Small RNAs have vital roles in numerous aspects of plant biology. Deciphering their precise contributions requires knowledge of a small RNA's spatiotemporal pattern of accumulation. The in situ hybridization protocol described here takes advantage of locked nucleic acid (LNA) oligonucleotide probes to visualize small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within 2-6 days