Пряма регенерація мікропагонів з листкових дисків ківі (Actinidia delisiosa (Chev.) Liang, Ferguson) в умовах in vitro

The method of direct regeneration of microshoots from leaf disks of kiwi fruit is developed. The factors influencing morphogenetic potency of leaf disks of 4 kiwi fruit cultivars (Bruno, Monti, Tomuri, Sanishton) in condition in vitro are determined. The genetically uniform plant material is obtained.Розроблено спосіб прямої регенерації мікропагонів з листкових дисків ківі. Визначено фактори, що впливають на морфогенетичні потенції листкових дисків 4 сортів ківі (Бруно, Монті, Томурі, Саніштон) в умовах in vitro. Отримано генетично однорідний рослинний матеріал

Caloric restriction and the nutrient-sensing protein kinase TOR1 alter the pattern of protein phosphorylation in quiescent and non-quiescent cells of Saccharomyces cerevisiae

The application of yeast as a model organism for studying eukaryotic pathways, notably mechanisms and processes of chronological aging, has been recognized for decades. In fact, several signalling pathways of longevity regulation are conserved across phyla; humans (and other mammals) have orthologs and homologs of yeast proteins integrated into these pathways. One of such pathways is the TOR pathway that responds to nutrient levels, notably via TORC1 (a complex with protein kinase activity; contains TOR1 as a core protein). My thesis taps into both of those advantageous properties of Saccharomyces cerevisiae: its ease of culturing for chronological aging studies, and well annotated proteome. I study the chronologically aging quiescent and non-quiescent cell populations under caloric restriction or not using wild-type or tor1 single gene deletion mutant strains. I use quantitative phosphoproteomics – by means of mass spectrometry – to assess the differences and similarities between different cell populations. Caloric restriction has previously been shown to extend the chronological lifespan of yeast and other organisms. Reduced TOR1 activity (such as via inhibitors or by gene deletion) is also shown to extend yeast chronological lifespan in literature. Quiescence, an ability of a nutrient-limited post-mitotic cell to re-enter the cell cycle when the nutrient supply is restored, is also a lifespan-extending process. Combining these factors, I compared the phosphoproteomes of quiescent and non-quiescent yeast cells limited or not limited in calorie supply and having or lacking the TOR1 protein. I found that both the diet and the state of quiescence have significant effect on the phosphorylation of proteins. Moreover, I found that a single-gene-deletion mutation that eliminates the TOR1 protein has a significant impact on both the state of quiescence and the cell phosphoproteome

Orthodox actors and equal opportunities policies in the Republic of Moldova in the context of the transformation of post-Soviet societies

This article examines how the key Orthodox actors in Moldova have reacted to challenging equal opportunities legislation. The author suggests, on the basis of an economic approach to religion, that under the conditions of a deregulated religious market they use various strategies to promote their agendas. The Moldovan Orthodox Church (the Metropolitanate of Moldova), autonomous within the Russian Orthodox Church, previously relied on making private bargains with the government; but this policy ended with the adoption of the 2013 Law on Ensuring Equality in the Republic of Moldova. Now the Metropolitanate tries to assimilate the strategies of direct action, but without success. The so-called ‘non-mentioning’ radicals, technically being part of the Metropolitanate of Moldova, but not praying for its bishop, are involved in direct political activism – from setting up protest camps to street fighting – to confront de-stigmatization of homosexuality. The Bessarabian Metropolitanate of the Romanian Orthodox Church utilizes the strategies of European ‘public’ churches and gains influence through performing some useful social functions. This article concludes that all actors have their own advantages and weaknesses; nevertheless, so far the Metropolitanate of Moldova remains the strongest; while the other competitors are serving specific religious niches, it is this body which still possesses the potential to influence society more broadly

Disambiguation of Taxonomy Markers in Context: Russian Nouns

Proceedings of the 17th Nordic Conference of Computational Linguistics NODALIDA 2009. Editors: Kristiina Jokinen and Eckhard Bick. NEALT Proceedings Series, Vol. 4 (2009), 111-117. © 2009 The editors and contributors. Published by Northern European Association for Language Technology (NEALT) http://omilia.uio.no/nealt . Electronically published at Tartu University Library (Estonia) http://hdl.handle.net/10062/9206

Fundamentalisation of philological education in the medical university

В статье представлены общие положения фундаментализации филологического образования в медицинском университете. Обоснована необходимость развития системы филологического образования. Дано определение филологического образования в медицинском вузе.The general ideas of fundamentalisation of philological education in the medical university are presented in the paper. The necessity of the development of philological education system is proved. The definition of philological education in the medical university was given

Distance learning technologies for teaching a foreign language in medical school

В работе рассмотрены аспекты, связанные с внедрением дистанционных образовательных технологий для обучения иностранным языкам в вузе. Представлен опыт зарубежных исследователей по применению электронных образовательных ресурсов в медицинском образовании. Описано современное состояние дел по использованию дистанционных образовательных технологий при обучении иностранному языку в российских вузах. Представлен опыт организации смешанного обучения иностранному языку студентов-медиков на основе электронного образовательного ресурса.The paper presents issues associated with the introduction of distance learning technologies to teach a foreign language at the university. The review of foreign studies concerning e-learning resources in medical education is presented. The current problems of introduction of distance learning technologies to teach Russian students a foreign language are determined. The experience of blended learning for teaching medical students a foreign language is described