The diet of feral goats (Capra hircus L.) in the Rimu-Rata-Kamahi Forest of Mount Egmont [microform] : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Zoology at Massey University

The diet of the feral goat (Capra hircus) in rimu-rata-kamahi forest on Mount Egmont was examined by sorting monthly rumen samples for one year. Seasonal changes in diet, relative plant palatabilities, differential fragmentation and digestion rates of plant species, nitrogen and mineral levels in principal foods, and several aspects of population biology were measured. Results show that individual goats contain at least 19 plant species on average and some more than 30. Presumably, they eat about this number daily. However, just two species (Asplenium bulbiferum and Ripogonum scandens) make up 44.8% of the total amount eaten over the year. There are significant seasonal changes in the amounts eaten for Coprosma grandifolius, Coprosma tenuifolia, Griselinia littoralis, Melicytus ramiflorus, Ripogonum scandens (fruit and vine) and Weinmannia racemosa. Goats clearly select or reject different plant species. Thus use of species is largely independent of availability. The most preferred foods are probably Schefflera digitata and Ripogonum scandens fruit and vine. In contrast the very abundant Microlaena spp., Uncinia spp., moss, Alsophila smithii and especially Blechnum fluviatile are among the most unpalatable. The low and probably variable availability of many species within the study area obscures their seasonal trends and palatability ratings. Asplenium bulbiferum, and probably Melicytus ramiflorus, are underestimated in the diet, whereas Ripogonum scandens vine may be overestimated. However, the magnitude of error is not sufficient to be a problem in this study. There is no obvious correlation between diet selection and the levels in plants of N, K, Ca, Mg, P, s, Cu, Zn, Fe and Mn. Only Na is deficient enough to possibly be selected for and highest levels occur in the very palatable Schefflera digitata. Age structure, body condition and reproductive data suggest a predominantly young, healthy population that is reproducing rapidly

Absence of Face-specific Cortical Activity in the Complete Absence of Awareness: Converging Evidence from Functional Magnetic Resonance Imaging and Event-related Potentials

In this study, we explored the neural correlates of perceptual awareness during a masked face detection task. To assess awareness more precisely than in previous studies, participants employed a 4-point scale to rate subjective visibility. An event-related fMRI and a high-density ERP study were carried out. Imaging data showed that conscious face detection was linked to activation of fusiform and occipital face areas. Frontal and parietal regions, including the pre-SMA, inferior frontal sulcus, anterior insula/frontal operculum, and intraparietal sulcus, also responded strongly when faces were consciously perceived. In contrast, no brain area showed face-selective activity when participants reported no impression of a face. ERP results showed that conscious face detection was associated with enhanced N170 and also with the presence of a second negativity around 300 msec and a slow positivity around 415 msec. Again, face-related activity was absent when faces were not consciously perceived. We suggest that, under conditions of backward masking, ventral stream and fronto-parietal regions show similar, strong links of face-related activity to conscious perception and stress the importance of a detailed assessment of awareness to examine activity related to unseen stimulus events

Developing Novel Host-Based Therapies Targeting Microbicidal Responses in Macrophages and Neutrophils to Combat Bacterial Antimicrobial Resistance

Antimicrobial therapy has provided the main component of chemotherapy against bacterial pathogens. The effectiveness of this strategy has, however, been increasingly challenged by the emergence of antimicrobial resistance which now threatens the sustained utility of this approach. Humans and animals are constantly exposed to bacteria and have developed effective strategies to control pathogens involving innate and adaptive immune responses. Impaired pathogen handling by the innate immune system is a key determinant of susceptibility to bacterial infection. However, the essential components of this response, specifically those which are amenable to re-calibration to improve host defense, remain elusive despite extensive research. We provide a mini-review focusing on therapeutic targeting of microbicidal responses in macrophages and neutrophils to de-stress reliance on antimicrobial therapy. We highlight pre-clinical and clinical data pointing toward potential targets and therapies. We suggest that developing focused host-directed therapeutic strategies to enhance “pauci-inflammatory” microbial killing in myeloid phagocytes that maximizes pathogen clearance while minimizing the harmful consequences of the inflammatory response merits particular attention. We also suggest the importance of One Health approaches in developing host-based approaches through model development and comparative medicine in informing our understanding of how to deliver this strategy

Phage Hunting at the University of Mary Washington: Genome Annotation of Hari and JackRabbit

Bacillus thuringiensis subspecies Kurstaki (BTK) is often used as a microbial insecticide for pest control and as a simulant for Bacillus anthracis in biowarfare and bioterrorism studies. Students in 2021 Phage Hunters class at University of Mary Washington isolated nine bacteriophages using the host Bacillus thuringiensis subspecies Kurstaki. Two phages, Hari and Jackrabbit, were sent to SEAPHAGES for sequencing are currently being annotated in the lab during the Spring semester. Hari was found in a soil sample obtained from King George, VA while JackRabbit was isolated from Linton, VA. Both samples were isolated from enriched cultures. Hari has a genome length of 161,978 bp, which auto-annotated with 286 features, and a direct terminal repeat of 2,633 bp. Hari is most similar to DIGNKC, SBP8a and PPIsBest by BLAST. JackRabbit has a genome length of 161,552 bp, which auto-annotated with 288 features, and a direct terminal repeat of 2,821 bp

Multiple locus VNTR analysis highlights that geographical clustering and distribution of Dichelobacter nodosus, the causal agent of footrot in sheep, correlates with inter-country movements

Dichelobacter nodosus is a Gram-negative, anaerobic bacterium and the causal agent of footrot in sheep. Multiple locus variable number tandem repeat (VNTR) analysis (MLVA) is a portable technique that involves the identification and enumeration of polymorphic tandem repeats across the genome. The aims of this study were to develop an MLVA scheme for D. nodosus suitable for use as a molecular typing tool, and to apply it to a global collection of isolates. Seventy-seven isolates selected from regions with a long history of footrot (GB, Australia) and regions where footrot has recently been reported (India, Scandinavia), were characterised. From an initial 61 potential VNTR regions, four loci were identified as usable and in combination had the attributes required of a typing method for use in bacterial epidemiology: high discriminatory power (D > 0.95), typeability and reproducibility. Results from the analysis indicate that D. nodosus appears to have evolved via recombinational exchanges and clonal diversification. This has resulted in some clonal complexes that contain isolates from multiple countries and continents; and others that contain isolates from a single geographic location (country or region). The distribution of alleles between countries matches historical accounts of sheep movements, suggesting that the MLVA technique is sufficiently specific and sensitive for an epidemiological investigation of the global distribution of D. nodosus

Adaptive Wolf Management: The Regulated Public Harvest Component

Montana’s wolf (Canis lupus) conservation and management plan is based on adaptive management principles and includes regulated public harvest as a population management tool. The need and opportunity to implement public harvest in 2008, 2009, and 2010 required Montana Fish, Wildlife and Parks (FWP) to develop a stepped down adaptive management framework specific to harvest. For 2008 and 2009, FWP set modest objectives: implement a harvest, maintain a recovered population, and begin the learning process to inform development of future hunting regulations and quotas. In 2010, FWP used a formal Structured Decision Making Process to more clearly define priorities and challenges of setting a wolf season, outline objectives of a successful season, and evaluate consequences and trade-offs between alternative management actions. For all years, FWP used a modeling process to simulate a wide range of harvest rates across three harvest units and to predict harvest effects on the minimum number of wolves, packs and breeding pairs. Model inputs were derived from minimum wolf numbers observed in the field. Modeling allowed consideration of a range of harvest quotas, predicted outcomes, and risk that harvest could drive the population below federally-required minimums. It also facilitated explicit consideration of how well a particular quota achieved objectives and how to adapt future regulations and quotas. Legal challenges to federal delisting restricted implementation of the first fair chase hunting season to 2009. Montana’s wolf population is securely recovered, despite the dynamic political and legal environments. Regardless, FWP remains committed to a scientific, data-driven approach to adaptive management

Association of flavonoids and flavonoid-rich foods with all-cause mortality: The Blue Mountains Eye Study

BACKGROUND: Higher intakes of flavonoids provide health benefits, however, the importance of each flavonoid class and which population groups may receive the greatest protection from higher flavonoid intake warrants further investigation. OBJECTIVE: To explore the associations of flavonoid and flavonoid-rich wholefood intakes with all-cause mortality and the moderating effects of early mortality risk factors. DESIGN: The study included 2349 participants of The Blue Mountains Eye Study, with a mean ± SD age at baseline of 64.7 ± 9.2 years. Flavonoid intake was calculated from baseline food frequency questionnaires using US Department of Agriculture food composition databases. Associations were examined using adjusted Cox proportional hazards models. RESULTS: After 14 years of follow-up, 677 participants died. There was a flavonoid threshold effect with the greatest risk reduction seen between low and moderate intakes of total flavonoids, flavonoid classes and flavonoid-rich foods. Amongst the whole cohort, participants in the highest tertile of anthocyanidin intake had a significantly lower risk of all-cause mortality [multivariable adjusted HR (95%CI): 0.76 (0.61, 0.94)] when compared to those in the lowest tertile. Amongst participants with at least one early mortality risk factor (smoking, high alcohol consumption, no regular exercise or obesity), risk of all-cause mortality was lower in those in the highest intake tertile for total flavonoids [adjusted HR: 0.77 (0.59, 1.00)], flavan-3-ols [0.75 (0.58, 0.98)], anthocyanidins [0.70 (0.54, 0.92)], and proanthocyanidins [0.69 (0.52, 0.92)], compared to those in the lowest tertile. No similar associations were observed among those without any risk factors. Similarly, consumption of apples, tea and the individual flavonoid compounds, quercetin and epicatechin, were associated with a lower risk of all-cause mortality among participants with at least one risk factor, but not amongst other participants. CONCLUSION: Moderate to high intakes of flavonoids and certain flavonoid subclasses may provide health benefits, particularly for individuals with at least one early mortality risk factor

Development of a parental feeding goal measure: the family mealtime goals questionnaire

It is well established that parents’ feeding practices predict children’s eating behaviors. However, there has been little research into parents’ mealtime goals – their desired outcomes for family mealtimes. These goals, and potential conflicts between them, may be important both in explaining parents’ feeding practices and improving children’s eating behaviors, as health behavior change is more likely to be achieved by programmes and interventions that are aligned with an individual’s goals. The objectives of this study were to develop a reliable and valid measure that captures parental mealtime goals, and to describe parents’ endorsement of these goals. Online questionnaire methods were used to design and test the Family Mealtime Goals Questionnaire with 1,140 parents and carers of at least one child aged from 1 to 16 years. Exploratory qualitative analysis, Principal Components Analysis, Confirmatory Factor Analysis and test-retest analysis (using intraclass correlations) were conducted to establish the psychometric properties of the instrument. An 18-item questionnaire was produced with seven dimensions: stress/conflict avoidance, homemade food, shared family food, family involvement in mealtimes, price, occasional treats, and high/low fat regulation. Some differences were found in the goal structure of parents of children of different ages but stress/conflict avoidance was the most strongly endorsed mealtime goal for all age groups. The Family Mealtime Goals Questionnaire provides a useful measure of parents’ feeding motivations. It will facilitate large-scale research into the relationships between parents' feeding goals and practices and could inform the design of more effective healthy eating interventions that target specific feeding goals

Outbreak of Pneumonia in the Setting of Fatal Pneumococcal Meningitis among US Army Trainees: Potential Role of Chlamydia pneumoniae Infection

<p>Abstract</p> <p>Background</p> <p>Compared to the civilian population, military trainees are often at increased risk for respiratory infections. We investigated an outbreak of radiologically-confirmed pneumonia that was recognized after 2 fatal cases of serotype 7F pneumococcal meningitis were reported in a 303-person military trainee company (Alpha Company).</p> <p>Methods</p> <p>We reviewed surveillance data on pneumonia and febrile respiratory illness at the training facility; conducted chart reviews for cases of radiologically-confirmed pneumonia; and administered surveys and collected nasopharyngeal swabs from trainees in the outbreak battalion (Alpha and Hotel Companies), associated training staff, and trainees newly joining the battalion.</p> <p>Results</p> <p>Among Alpha and Hotel Company trainees, the average weekly attack rates of radiologically-confirmed pneumonia were 1.4% and 1.2% (most other companies at FLW: 0-0.4%). The pneumococcal carriage rate among all Alpha Company trainees was 15% with a predominance of serotypes 7F and 3. <it>Chlamydia pneumoniae </it>was identified from 31% of specimens collected from Alpha Company trainees with respiratory symptoms.</p> <p>Conclusion</p> <p>Although the etiology of the outbreak remains unclear, the identification of both <it>S. pneumoniae </it>and <it>C. pneumoniae </it>among trainees suggests that both pathogens may have contributed either independently or as cofactors to the observed increased incidence of pneumonia in the outbreak battalion and should be considered as possible etiologies in outbreaks of pneumonia in the military population.</p

Coordinated Primary Health Care for Refugees: A Best Practice Framework for Australia

Australia permanently resettles over 20,000 refugees each year. Our team reviewed the international evidence and conducted new research to develop a framework for the delivery of accessible and coordinated primary health care to this vulnerable group.The research reported in this paper is a project of the Australian Primary Health Care Research Institute which is supported by a grant from the Australian Government Department of Health and Ageing under the Primary Health Care Research Evaluation and Development Strategy