Biosurfactants and synthetic surfactants in bioelectrochemical systems: a mini-review

Bioelectrochemical systems (BESs) are ruled by a complex combination of biological and abiotic factors. The interplay of these factors determines the overall efficiency of BES in generating electricity and treating waste. The recent progress in bioelectrochemistry of BESs and electrobiotechnology exposed an important group of compounds, which have a significant contribution to operation and efficiency: surface-active agents, also termed surfactants. Implementation of the interfacial science led to determining several effects of synthetic and natural surfactants on BESs operation. In high pH, these amphiphilic compounds prevent the cathode electrodes from biodeterioration. Through solubilization, their presence leads to increased catabolism of hydrophobic compounds. They interfere with the surface of the electrodes leading to improved biofilm formation, while affecting its microarchitecture and composition. Furthermore, they may act as quorum sensing activators and induce the synthesis of electron shuttles produced by electroactive bacteria. On the other hand, the bioelectrochemical activity can be tailored for new, improved biosurfactant production processes. Herein, the most recent knowledge on the effects of these promising compounds in BESs is discussed

Customized Woven Carbon Fiber Electrodes for Bioelectrochemical Systems—A Study of Structural Parameters

Commercial carbon fiber (CF) fabrics are popular electrode materials for bioelectrochemical systems (BES), but are usually not optimized for the specific application. This study investigates BES-relevant material characteristics on fabric level, such as weave types and weave parameters. The two contrasting weave types plain and leno weave were characterized with respect to their envisaged application types: 1) BES with mainly advective flow regimes and 2) stirred systems, which could benefit from fluid flow through a fabric electrode. Experiments with batch and continuously fed pure cultures of Geobacter sulfurreducens PCA and Shewanella oneidensis MR-1 reveal that µm-scale electrode topologies are of limited use for the thick biofilms of G. sulfurreducens , but can boost S. oneidensis ’ current generation especially in batch and fed-batch reactors. For advective flow regimes, deeper layers of biofilm inside microporous electrodes are often mass transport limited, even with thin biofilms of S. oneidensis . Therefore, low porosity plain weave electrodes for advective flow operation as in wastewater treating BES should be thin and flat. A trade-off between maximized current density and electrode material utilization exists, which is optimized exemplarily for an advective flow operation. For stirred BES of biotechnological applications, a flow-through of electrolyte is desired. For this, leno weave fabrics with pores at cm-scale are produced from 100% CF for the first time. In a preliminary evaluation, they outperform plain weave fabrics. Mass transfer investigations in stirred BES demonstrate that the large pores enable efficient electrode utilization at lower power input in terms of stirring speed

Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production

Sufficient supply of oxygen is amajor bottleneck in industrial biotechnological synthesis. One example is the heterologous production of rhamnolipids using Pseudomonas putida KT2440. Typically, the synthesis is accompanied by strong foam formation in the reactor vessel hampering the process. It is caused by the extensive bubbling needed to sustain the high respirative oxygen demand in the presence of the produced surfactants. One way to reduce the oxygen requirement is to enable the cells to use the anode of a bioelectrochemical system (BES) as an alternative sink for their metabolically derived electrons. We here used a P. putida KT2440 strain that interacts with the anode using mediated extracellular electron transfer via intrinsically produced phenazines, to performheterologous rhamnolipid production under oxygen limitation. The strain P. putida RL-PCA successfully produced 30.4 � 4.7mg/Lmono-rhamnolipids togetherwith 11.2 � 0.8mg/L of phenazine-1-carboxylic acid (PCA) in 500-mL benchtop BES reactors and 30.5 � 0.5 mg/L rhamnolipids accompanied by 25.7 � 8.0 mg/L PCA in electrode containing standard 1-L bioreactors. Hence, this study marks a first proof of concept to produce glycolipid surfactants in oxygen-limited BES with an industrially relevant strain

Transcriptional Analysis of Shewanella oneidensis MR-1 with an Electrode Compared to Fe(III)Citrate or Oxygen as Terminal Electron Acceptor

Shewanella oneidensis is a target of extensive research in the fields of bioelectrochemical systems and bioremediation because of its versatile metabolic capabilities, especially with regard to respiration with extracellular electron acceptors. The physiological activity of S. oneidensis to respire at electrodes is of great interest, but the growth conditions in thin-layer biofilms make physiological analyses experimentally challenging. Here, we took a global approach to evaluate physiological activity with an electrode as terminal electron acceptor for the generation of electric current. We performed expression analysis with DNA microarrays to compare the overall gene expression with an electrode to that with soluble iron(III) or oxygen as the electron acceptor and applied new hierarchical model-based statistics for the differential expression analysis. We confirmed the differential expression of many genes that have previously been reported to be involved in electrode respiration, such as the entire mtr operon. We also formulate hypotheses on other possible gene involvements in electrode respiration, for example, a role of ScyA in inter-protein electron transfer and a regulatory role of the cbb3-type cytochrome c oxidase under anaerobic conditions. Further, we hypothesize that electrode respiration imposes a significant stress on S. oneidensis, resulting in higher energetic costs for electrode respiration than for soluble iron(III) respiration, which fosters a higher metabolic turnover to cover energy needs. Our hypotheses now require experimental verification, but this expression analysis provides a fundamental platform for further studies into the molecular mechanisms of S. oneidensis electron transfer and the physiologically special situation of growth on a poised-potential surface

Ramucirumab plus docetaxel versus placebo plus docetaxel in patients with locally advanced or metastatic urothelial carcinoma after platinum-based therapy (RANGE): a randomised, double-blind, phase 3 trial

Few treatments with a distinct mechanism of action are available for patients with platinum-refractory advanced or metastatic urothelial carcinoma. We assessed the efficacy and safety of treatment with docetaxel plus either ramucirumab-a human IgG1 VEGFR-2 antagonist-or placebo in this patient population

Measurement Techniques to Resolve and Control Population Dynamics of Mixed-Culture Processes.

Schlembach I, Grünberger A, Rosenbaum MA, Regestein L. Measurement Techniques to Resolve and Control Population Dynamics of Mixed-Culture Processes. Trends in Biotechnology. 2021.Microbial mixed cultures are gaining increasing attention as biotechnological production systems, since they offer a large but untapped potential for future bioprocesses. Effects of secondary metabolite induction and advantages of labor division for the degradation of complex substrates offer new possibilities for process intensification. However, mixed cultures are highly complex, and, consequently, many biotic and abiotic parameters are required to be identified, characterized, and ideally controlled to establish a stable bioprocess. In this review, we discuss the advantages and disadvantages of existing measurement techniques for identifying, characterizing, monitoring, and controlling mixed cultures and highlight promising examples. Moreover, existing challenges and emerging technologies are discussed, which lay the foundation for novel analytical workflows to monitor mixed-culture bioprocesses. Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved