33 research outputs found
Calcium-dependent protein kinase OsCPK10 mediates both drought tolerance and blast disease resistance in rice plants
OsCPK10 mediates drought tolerance and blast disease resistance in rice plants by enhancing their reactive oxygen species scavenging capacity and so reducing oxidative damage associated with both stresses. Plant growth and productivity is negatively affected by different stresses. Most stresses trigger calcium signals that initiate acclimation responses in plants. The multigene family of plant calcium-dependent protein kinases (CPKs) functions in multiple stress responses by transducing calcium signals into phosphorylation events. This work reports that the OsCPK10 isoform positively mediates tolerance to different stresses in rice plants by enhancing their antioxidant capacity and protecting them from reactive oxygen species (ROS) damage, with the uncontrolled generation of ROS being a common feature of these stresses. Here, we show that the constitutive accumulation of an HA-tagged OsCPK10 full-length protein enhances the hydrogen peroxide detoxifying capacity of rice plants during desiccation. This is achived by modulating the accumulation of catalase proteins, which reduces the extent of lipid peroxidation and protects the integrity of cell membranes, resulting in drought tolerance. OsCPK10HA accumulation also confers blast disease resistance by interfering with fungal necrotrophic growth via a reduction in the accumulation of hydrogen peroxide. Furthermore, we show by bimolecular complementation assays that OsCPK10 is a plasma membrane protein that physically interacts in vivo with catalase A. OsCPK10 therefore appears to be a good molecular target to improve tolerance to abiotic stresses as well as to blast disease, which limit rice crop productivity
Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
Background: BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. Here we report the production and recovery of a bioactive BP178 peptide using rice seeds as biofactories. - Results: a synthetic gene encoding the BP178 peptide was prepared and introduced in rice plants. The gene was efficiently expressed in transgenic rice under the control of an endosperm-specific promoter. Among the three endosperm-specific rice promoters (Glutelin B1, Glutelin B4 or Globulin 1), best results were obtained when using the Globulin 1 promoter. The BP178 peptide accumulated in the seed endosperm and was easily recovered from rice seeds using a simple procedure with a yield of 21 μg/g. The transgene was stably inherited for at least three generations, and peptide accumulation remained stable during long term storage of transgenic seeds. The purified peptide showed in vitro activity against the bacterial plant pathogen Dickeya sp., the causal agent of the dark brown sheath rot of rice. Seedlings of transgenic events showed enhanced resistance to the fungal pathogen Fusarium verticillioides, supporting that the in planta produced peptide was biologically active. -Conclusions: the strategy developed in this work for the sustainable production of BP178 peptide using rice seeds as biofactories represents a promising system for future production of peptides for plant protection and possibly in other fields
Nano-engineering of ketorolac tromethamine platforms for ocular treatment of inflammatory disorders
Aim: The development and optimization of Ketorolac tromethamine-loaded polylactic-co-glycolic acid nanoparticles (KT-NPs) for the treatment of inflammatory processes of the eye. Materials & methods: KT-NPs were developed by factorial design and characterized by assessing their physicochemical properties. Biopharmaceutical behavior studies, ocular tolerance, anti-inflammatory efficacy and bioavailability tests were performed on pigs. Results: Optimized KT-NPs of 112 nm, narrow distribution with encapsulation efficiency near 100% were obtained. KT release followed the Weibull model and there was significantly greater retention in the cornea and sclera than in the commercial reference. KT-NPs showed no signs of ocular irritancy and similar anti-inflammatory efficacy to the commercial reference. Conclusion: KT-NPs were a suitable alternative for the treatment of inflammatory disorders of the anterior and posterior segments of the eye as an alternative to conventional topical formulations
Calcium-dependent protein kinase OsCPK10 mediates both drought tolerance and blast disease resistance in rice plants
OsCPK10 mediates drought tolerance and blast disease resistance in rice plants by enhancing their reactive oxygen species scavenging capacity and so reducing oxidative damage associated with both stresses. Plant growth and productivity is negatively affected by different stresses. Most stresses trigger calcium signals that initiate acclimation responses in plants. The multigene family of plant calcium-dependent protein kinases (CPKs) functions in multiple stress responses by transducing calcium signals into phosphorylation events. This work reports that the OsCPK10 isoform positively mediates tolerance to different stresses in rice plants by enhancing their antioxidant capacity and protecting them from reactive oxygen species (ROS) damage, with the uncontrolled generation of ROS being a common feature of these stresses. Here, we show that the constitutive accumulation of an HA-tagged OsCPK10 full-length protein enhances the hydrogen peroxide detoxifying capacity of rice plants during desiccation. This is achived by modulating the accumulation of catalase proteins, which reduces the extent of lipid peroxidation and protects the integrity of cell membranes, resulting in drought tolerance. OsCPK10HA accumulation also confers blast disease resistance by interfering with fungal necrotrophic growth via a reduction in the accumulation of hydrogen peroxide. Furthermore, we show by bimolecular complementation assays that OsCPK10 is a plasma membrane protein that physically interacts in vivo with catalase A. OsCPK10 therefore appears to be a good molecular target to improve tolerance to abiotic stresses as well as to blast disease, which limit rice crop productivity
Biological differences related to cultural variability during the Neolithic in a micro-geographical area of the Iberian Peninsula
This paper presents dental morphological data of Neolithic, Chalcolithic and Bronze Age populations from the Catalan Pre-Pyrenean area. The Neolithic group, in particular, differs from those of surrounding areas in its funerary culture: the building of cists, which is not present in the Sepulcres de Fossa Culture. A minimum number of 118 individuals from this area were studied for this work, and the data were compared with those of other Iberian and European groups. The results indicate that the two micro-regional groups from the Catalan area (Pre-Pyrenean and Pre-Coastal) were biologically different during the Neolithic and the Chalcolithic, but not in the Bronze Age, when they also appeared to be more homogeneous culturally. In addition, both areas differ biologically from coetaneous Italian groups, although those closer to the coast show slightly smaller differences. Finally, the Bronze Age groups also present fewer differences with regard to the Italian Bronze Age’s group. Therefore, the results suggest that the Catalan Neolithic population had two separate origins, related to cultural patterns, and that differences between the groups decreased within time, probably due to trade-related activities. Moreover, the fact that the difference with Italian populations decreased during the Bronze Age suggests major population movements through the Mediterranean that would affect the biological composition of the human groups.We would like to thank all the staff from "Museu Diocesal i Comarcal de Solsona" for the assistance they have provided us with during our works. Moreover, we would like to thank Crystal Rodgers and UALT (UAB) for the revision of the English language in the manuscript. This research has been funded by the projects HAR2011-23149, HAR2015-67323-C2-1-P of Ministerio de Ciencia e Innovación and SGR2014-1420 consolidated research group by Generalitat de Catalunya.Peer reviewe
{'en_US': 'Application of the nursing process in hemodialised pregnant women', 'es_ES': 'Aplicación del proceso enfermero en gestante hemodializada'}
La frecuencia de las gestaciones en mujeres en tratamiento con hemodiálisis está incrementándose en los últimos años. El aumento de fertilidad en estas pacientes se debe a la mejora de la eficacia de la diálisis, junto con el manejo clínico de la insuficiencia renal. Sin embargo, el embarazo de las pacientes hemodializadas sigue siendo un reto, debido a frecuentes complicaciones como la preeclampsia, la prematuridad severa, el polihidramnios, etc. Actualmente, se constata el ascenso de la tasa de supervivencia fetal y la disminución de la morbi-mortalidad materno-fetal.
Presentamos el caso de una gestante en tratamiento con hemodiálisis que presenta una amenaza de parto prematuro a la 27+2 semanas de gestación. Se han formulado diagnósticos enfermeros siguiendo la taxonomía NANDA-NOC-NIC, con la finalidad de visibilizar la necesidad de un adecuado plan de cuidados en estas pacientes
Aplicación del proceso enfermero en gestante hemodializada
Resumen La frecuencia de las gestaciones en mujeres en tratamiento con hemodiálisis está incrementándose en los últimos años. El aumento de fertilidad en estas pacientes se debe a la mejora de la eficacia de la diálisis, junto con el manejo clínico de la insuficiencia renal. Sin embargo, el embarazo de las pacientes hemodializadas sigue siendo un reto, debido a frecuentes complicaciones como la preeclampsia, la prematuridad severa, el polihidramnios, etc. Actualmente, se constata el ascenso de la tasa de supervivencia fetal y la disminución de la morbi-mortalidad materno-fetal. Presentamos el caso de una gestante en tratamiento con hemodiálisis que presenta una amenaza de parto prematuro a la 27+2 semanas de gestación. Se han formulado diagnósticos enfermeros siguiendo la taxonomía NANDA-NOC-NIC, con la finalidad de visibilizar la necesidad de un adecuado plan de cuidados en estas pacientes
Production of Biologically Active Cecropin A Peptide in Rice Seed Oil Bodies
Cecropin A is a natural antimicrobial peptide that exhibits fast and potent activity against a broad spectrum of pathogens and neoplastic cells, and that has important biotechnological applications. However, cecropin A exploitation, as for other antimicrobial peptides, is limited by their production and purification costs. Here, we report the efficient production of this bioactive peptide in rice bran using the rice oleosin 18 as a carrier protein. High cecropin A levels were reached in rice seeds driving the expression of the chimeric gene by the strong embryo-specific oleosin 18 own promoter, and targeting the peptide to the oil body organelle as an oleosin 18-cecropin A fusion protein. The accumulation of cecropin A in oil bodies had no deleterious effects on seed viability and seedling growth, as well as on seed yield. We also show that biologically active cecropin A can be easily purified from the transgenic rice seeds by homogenization and simple flotation centrifugation methods. Our results demonstrate that the oleosin fusion technology is suitable for the production of cecropin A in rice seeds, which can potentially be extended to other antimicrobial peptides to assist their exploitation
Rice seeds as biofactories of rationally designed and cell-penetrating antifungal PAF peptides
PAFs are short cationic and tryptophan-rich synthetic peptides with cell-penetrating antifungal activity. They show potent and selective killing activity against major fungal pathogens and low toxicity to other eukaryotic and bacterial cells. These properties make them a promising alternative to fulfill the need of novel antifungals with potential applications in crop protection, food preservation, and medical therapies. However, the difficulties of cost-effective manufacturing of PAFs by chemical synthesis or biotechnological production in microorganisms have hampered their development for practical use. This work explores the feasibility of using rice seeds as an economical and safe production system of PAFs. The rationally designed PAF102 peptide with improved antifungal properties was selected for assessing PAF biotechnological production. Two different strategies are evaluated: (1) the production as a single peptide targeted to protein bodies and (2) the production as an oleosin fusion protein targeted to oil bodies. Both strategies are designed to offer stability to the PAF peptide in the host plant and to facilitate its downstream purification. Our results demonstrate that PAF does not accumulate to detectable levels in rice seeds when produced as a single peptide, whereas it is successfully produced as fusion protein to the Oleosin18, up to 20 μg of peptide per gram of grain. We show that the expression of the chimeric Ole18-PAF102 gene driven by the Ole18 promoter results in the specific accumulation of the fusion protein in the embryo and aleurone layer of the rice seed. Ole18-PAF102 accumulation has no deleterious effects on seed yield, germination capacity, or seedling growth. We also show that the Oleosin18 protein serves as carrier to target the fusion protein to oil bodies facilitating PAF102 recovery. Importantly, the recovered PAF102 is active against the fungal phytopathogen Fusarium proliferatum. Altogether, our results prove that the oleosin fusion technology allows the production of PAF bioactive peptides to assist the exploitation of these antifungal compounds.This work was supported by SEPSAPE grants (Plant KBBE programme) EUI2008-03769 and EUI2008-03619 and by the grant BIO2015-68790-C2-2-R; through the “Severo Ochoa Programme for Centres of Excellence in R&D” (SEV-2015-0533) from Spanish Ministerio de Ciencia, Innovación y Universidades (co-financed FEDER funds); and by the CERCA Programme/Generalitat de Catalunya.Peer reviewe
Rice seeds as biofactories of rationally designed and cell-penetrating antifungal PAF peptides
PAFs are short cationic and tryptophan-rich synthetic peptides with cell-penetrating antifungal activity. They show potent and selective killing activity against major fungal pathogens and low toxicity to other eukaryotic and bacterial cells. These properties make them a promising alternative to fulfill the need of novel antifungals with potential applications in crop protection, food preservation, and medical therapies. However, the difficulties of cost-effective manufacturing of PAFs by chemical synthesis or biotechnological production in microorganisms have hampered their development for practical use. This work explores the feasibility of using rice seeds as an economical and safe production system of PAFs. The rationally designed PAF102 peptide with improved antifungal properties was selected for assessing PAF biotechnological production. Two different strategies are evaluated: (1) the production as a single peptide targeted to protein bodies and (2) the production as an oleosin fusion protein targeted to oil bodies. Both strategies are designed to offer stability to the PAF peptide in the host plant and to facilitate its downstream purification. Our results demonstrate that PAF does not accumulate to detectable levels in rice seeds when produced as a single peptide, whereas it is successfully produced as fusion protein to the Oleosin18, up to 20 μg of peptide per gram of grain. We show that the expression of the chimeric Ole18-PAF102 gene driven by the Ole18 promoter results in the specific accumulation of the fusion protein in the embryo and aleurone layer of the rice seed. Ole18-PAF102 accumulation has no deleterious effects on seed yield, germination capacity, or seedling growth. We also show that the Oleosin18 protein serves as carrier to target the fusion protein to oil bodies facilitating PAF102 recovery. Importantly, the recovered PAF102 is active against the fungal phytopathogen Fusarium proliferatum. Altogether, our results prove that the oleosin fusion technology allows the production of PAF bioactive peptides to assist the exploitation of these antifungal compounds