La mobilitat quotidiana a la regió metropolitana de Barcelona

Carme Miralles-Guasch i Laia Oliver-Frauca analitzen els hàbits de mobilitat de la regió metropolitana de Barcelona, on el patró de mobilitat no només és determinat per les característiques territorials i l'oferta d'infraestructures i serveis de transport existent, sinó que també és fortament condicionat pels trets personals de cada individu i de cada col·lectiu

Postmodern Postmortem. Intervenció en l´espai

The project is a subversive campaign (intervention in the public space for make art to everyone at the same time questioning the museum’s figure) consists of 7 printed in large size. We used advertising’s resources to sell an idea or message, create a fictitious brand and a slogan on each poster (guerrilla communication). The posters are composed by central illustration surrounded by a white background that gives clarity and professionalism, made with techniques like watercolour, pencil, pen and digital retouching. Along the illustration we find the brand “Postmodern Postmortem” this brand try to synthesize the occidental thought. Where the death of the ideologies is palpable in everywhere and the center of the life is the narcissistic hedonism. To reinforce this message is incorporated into each poster one slogan direct and simple, easy to understand (despite being written in English (universal language)). All the illustrations are zombies who wear a aesthetics hipster, they’ve missed some part of the body, they are empty, incomplete (critique of occidental society), but trying to be aesthetic, all the time, fighting for not fall into what is morbid (through wounds with a clean cut without blood). This project is critical to our occidental society and global consumerism, who has forgotten everything that happened and the direct relationship between their privileges and the deplorable conditions in the rest of the world. With the main aim to give pause to the viewer/ pedestrian.El projecte és una campanya subversiva (intervenció en l’espai públic per aconseguir un art per a tothom al mateix temps que qüestiona la figura actual del museu) composta per 7 cartells impresos en gran format. S’han utilitzat recursos propis de la publicitat per vendre una idea o transmetre el missatge, com la creació d’una marca fictícia i un eslògan en cada cartell. Els cartells estan formats per una il·lustració central envoltada per un fons blanc que li dona claredat i professionalitat, realitzada amb tècniques a l’aigua, llapis, bolígraf i retoc digital. Junt la il·lustració trobem la marca “Postmodern Postmortem”, amb un intent de reflectir la vessant de pensament occidental on la mort dels ideals es palpable per tot arreu i el centre és l’hedonisme narcisista. Per reforçar aquest missatge s’incorpora en cada cartell un eslògan directe i senzill, fàcil d’entendre (a pesar d’estar escrit en anglès (llengua universal)). Totes les il·lustracions són personatges zombies que vesteixen una estètica Hipster, als quals els falta alguna part del cos, estan buits, incomplets (crítica a la societat occidental), però intentant en tot moment que sigui estètic lluitant per no caure en lo mòrbid ( a través de ferides amb un tall net amb absència de sang). És un projecte crític amb la nostra societat actual occidental i global, hiperconsumista, que ha oblidat tot passat i la relació directa que hi ha entre els seus privilegis i les condicions deplorables a la resta del planeta. Amb la intenció principal de fer reflexionar a l’espectador/ vianant .Miralles Toledo, L. (2014). Postmodern Postmortem. Intervenció en l´espai. http://hdl.handle.net/10251/50239.Archivo delegad

Monitoring and Prognosis System Based on the ICF for People with Traumatic Brain Injury

The objective of this research is to provide a standardized platform to monitor and predict indicators of people with traumatic brain injury using the International Classification of Functioning, Disability and Health, and analyze its potential benefits for people with disabilities, health centers and administrations. We developed a platform that allows automatic standardization and automatic graphical representations of indicators of the status of individuals and populations. We used data from 730 people with acquired brain injury performing periodic comprehensive evaluations in the years 2006-2013. Health professionals noted that the use of color-coded graphical representation is useful for quickly diagnose failures, limitations or restrictions in rehabilitation. The prognosis System achieves 41% of accuracy and sensitivity in the prediction of emotional functions, and 48% of accuracy and sensitivity in the prediction of executive functions. This monitoring and prognosis system has the potential to: (1) save costs and time, (2) provide more information to make decisions, (3) promote interoperability, (4) facilitate joint decision-making, and (5) improve policies of socioeconomic evaluation of the burden of disease. Professionals found the monitoring system useful because it generates a more comprehensive understanding of health oriented to the profile of the patients, instead of their diseases and injuries

Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies

A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies—IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories Clearcoli (an engineered form of Escherichia coli) and Lactococcus lactis, mainly forming part of IBs and partially recovered under non-denaturing conditions. After the purification by affinity chromatography of solubilized MMP-9, four protein peaks were obtained. However, so far, the different conformational protein species forming part of IBs have not been isolated and characterized. Therefore, with the aim to link the physicochemical characteristics of the isolated peaks with their biological activity, we set up a methodological approach that included dynamic light scattering (DLS), circular dichroism (CD), and spectrofluorometric analysis confirming the separation of subpopulations of conformers with specific characteristics. In protein purification procedures, the detailed analysis of the individual physicochemical properties and the biological activity of protein peaks separated by chromatographic techniques is a reliable source of information to select the best-fitted protein populations.info:eu-repo/semantics/publishedVersio

'¡Ya esté!' Análisis de la traducción al español de Final Fantasy VII

Este Trabajo de Final de Grado trata los errores en la traducción al español del videojuego Final Fantasy VII y analiza los de mayor relevancia del primer disco del juego. Para ello, comparamos la traducción inglesa y el original japonés para averiguar de dónde proviene el error, y establecer una propuesta de traducción válida a partir del originalThis final project discusses the mistakes of the Spanish translation of the video game Final Fantasy VII and analyzes the most important errors of the first CD of the game. In order to do this, we compare it with the English translation and the original game in Japanese to find out where the errors come from, and establish a proposal for a suitable translation from the origina

Selecting subpopulations of high-quality protein conformers among conformational mixtures of recombinant bovine MMP-9 solubilized from inclusion bodies

Altres ajuts: CERCA Programme/Generalitat de CatalunyaA detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies-IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories Clearcoli (an engineered form of Escherichia coli) and Lactococcus lactis, mainly forming part of IBs and partially recovered under non-denaturing conditions. After the purification by affinity chromatography of solubilized MMP-9, four protein peaks were obtained. However, so far, the different conformational protein species forming part of IBs have not been isolated and characterized. Therefore, with the aim to link the physicochemical characteristics of the isolated peaks with their biological activity, we set up a methodological approach that included dynamic light scattering (DLS), circular dichroism (CD), and spectrofluorometric analysis confirming the separation of subpopulations of conformers with specific characteristics. In protein purification procedures, the detailed analysis of the individual physicochemical properties and the biological activity of protein peaks separated by chromatographic techniques is a reliable source of information to select the best-fitted protein populations

Potential of MMP-9 based nanoparticles at optimizing the cow dry period : pulling apart the effects of MMP-9 and nanoparticles

Altres ajuts: ICREA Academia awardThe cow dry period is a non-milking interval where the mammary gland involutes and regenerates to guarantee an optimal milk production in the subsequent lactation. Important bottlenecks such as the high risk of intramammary infections complicate the process. Antibiotics have been routinely used as a preventive treatment but the concerns about potential antibiotic resistance open a new scenario in which alternative strategies have to be developed. Matrix metalloproteinase-9 (MMP-9) is an enzyme able to degrade the extracellular matrix, triggering the involution and immune function of cow mammary gland. We have studied the infusion into the mammary gland of MMP-9 inclusion bodies as protein-based nanoparticles, demonstrating that 1.2 mg of MMP-9 enhanced the involution and immune function of the cow mammary gland. However, the comparison of the effects triggered by the administration of an active and an inactive form of MMP-9 led to conclude that the response observed in the bovine mammary gland was mainly due to the protein format but not to the biological activity of the MMP-9 embedded in the inclusion body. This study provides relevant information on the future use of protein inclusion bodies in cow mammary gland and the role of MMP-9 at dry-off

A new approach to obtain pure and active proteins from Lactococcus lactis protein aggregates

The production of pure and soluble proteins is a complex, protein-dependent and time-consuming process, in particular for those prone-to-aggregate and/or difficult-to-purify. Although Escherichia coli is widely used for protein production, recombinant products must be co-purified through costly processes to remove lipopolysaccharide (LPS) and minimize adverse effects in the target organism. Interestingly, Lactococcus lactis, which does not contain LPS, could be a promising alternative for the production of relevant proteins. However, to date, there is no universal strategy to produce and purify any recombinant protein, being still a protein-specific process. In this context and considering that L. lactis is also able to form functional protein aggregates under overproduction conditions, we explored the use of these aggregates as an alternative source of soluble proteins. In this study, we developed a widely applicable and economically affordable protocol to extract functional proteins from these nanoclusters. For that, two model proteins were used: mammary serum amyloid A3 (M-SAA3) and metalloproteinase 9 (MMP-9), a difficult-to-purify and a prone-to-aggregate protein, respectively. The results show that it is possible to obtain highly pure, soluble, LPS-free and active recombinant proteins from L. lactis aggregates through a cost-effective and simple protocol with special relevance for difficult-to-purify or highly aggregated proteins

Recombinant protein-based nanoparticles: Elucidating their inflammatory effects in vivo and their potential as a new therapeutic format

Bacterial inclusion bodies (IBs) are protein-based nanoparticles of a few hundred nanometers formed during recombinant protein production processes in different bacterial hosts. IBs contain active protein in a mechanically stable nanostructured format that has been broadly characterized, showing promising potential in different fields such as tissue engineering, protein replacement therapies, cancer, and biotechnology. For immunomodulatory purposes, however, the interference of the format immunogenic properties—intrinsic to IBs—with the specific effects of the therapeutic protein is still an uncovered gap. For that, active and inactive forms of the catalytic domain of a matrix metalloproteinase-9 (MMP-9 and mutMMP-9, respectively) have been produced as IBs and compared with the soluble form for dermal inflammatory effects in mmp9 knock-out mice. After protein injections in air-pouches in the mouse model, MMP-9 IBs induce local neutrophil recruitment and increase pro-inflammatory chemokine levels, lasting for at least two days, whereas the effects triggered by the soluble MMP-9 format fade out after 3 h. Interestingly, the IB intrinsic effects (mutMMP-9 IBs) do not last more than 24 h. Therefore, it may be concluded that IBs could be used for the delivery of therapeutic proteins, such as immunomodulating proteins while preserving their stability in the specific tissue and without triggering important unspecific inflammatory responses due to the protein format.info:eu-repo/semantics/publishedVersio