Participatory identification of climate-smart agriculture priorities

With the concept climate-smart agriculture (CSA) being relatively new, there is a need to test and develop practical and systematic methodologies and approaches for documenting and evaluating CSA practices in the field. The implementation of CCAFS’ Climate-Smart Villages (CSV) involves identifying, assessing and selecting climate-smart farming practices. This report contains three sections: (i) a framework for identifying and assessing CSA in the field with a long list of CSA indicators in identifying and monitoring CSA interventions; (ii) cost-benefit analysis of some selected climate-smart farming systems; and (iii) the participatory process of prioritizing CSA options with the villagers. The work builds on our experiences from the My Loi CSV and its scaling domains in Ky Anh district, Ha Tinh province, in the north-central region of Viet Nam

Activated Transcription of the Human Neuropeptide Y Gene in Differentiating SH-SY5Y Neuroblastoma Cells Is Dependent on Transcription Factors AP-1, AP-2Α, and NGFI

Activated transcription of the human neuropeptide Y gene ( NPY ) was investigated in SH-SY5Y neuroblastoma cells at the onset of sympathetic neuronal differentiation induced by 12- O -tetradecanoylphorbol 13-acetate (TPA) and serum or by nerve growth factor (NGF). As determined by transient expression, two NGF response elements (REs) were required for transcription induced by NGF in SH-SY5Y cells with stable expression of an exogenous NGF receptor TRK-A gene (SH-SY5Y/trk). TPA treatment in the presence of serum induced NPY transcription in both wild-type SH-SY5Y (SH-SY5Y/wt) and SH-SY5Y/trk cells. A TPA RE (TRE), overlapping the proximal NGF RE, was identified by expression of the v-Jun oncoprotein that enhanced NPY transcription. Suppression of TPA-induced NPY transcription was obtained by expression of a dominant negative Jun protein, selective protein kinase C inhibition, or introduction of a mutated TRE, whereas NGF-induced NPY transcription was inhibited to a lesser degree. The transcription factor AP-2Α was shown to bind cooperatively to the NPY promoter with either AP-1 or NGFI-A to the shared TRE and NGF RE and to the distal NGF RE, respectively. These results show that transcription factors AP-1, AP-2Α, and NGFI-A are involved in activated NPY transcription during the onset of neuronal differentiation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65864/1/j.1471-4159.1998.70051887.x.pd

Tissue-specific expression of the human neuropeptide Y gene in transgenic mice

Neuropeptide Y (NPY) is the most abundant neuropeptide detected in the mammalian brain, and is found throughout the central and peripheral nervous systems. This peptide is a proposed regulator of appetite, blood pressure, and pituitary hormone release. Previous experiments have demonstrated the ability of 5' sequences within the human NPY gene to promote transcription in cultured neuronal cells. To identify sequences in this gene that regulate tissue-specific expression, a NPY/CAT fusion gene, containing approximately 850 bp of NPY sequences, was microinjected into fertilized mouse ova. Five lines of transgenic mice were derived from these ova and several tissues from mice of each line were tested for transgene expression using the CAT assay. One line demonstrated X-chromosome-linked transmission of the transgene while the other lines demonstrated autosomally-linked transmission. Three lines demonstrated transgene expression with significant levels of CAT activity detectable only in tissues which have been shown to express endogenous NPY. One autosomally-linked line did not demonstrate significant levels of transgene activity because the transgene appeared to have undergone structural alteration during genomic integration. No transgene activity was detected in either male of female mice from the X-linked line, suggesting a positional regulation of the transgene locus other than X-inactivation in this line. The present research demonstrated the NPY regulatory sequences included i in pCATNPY[Delta]796 sufficiently directed tissue-appropriate gene expression in transgenic mice.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29991/1/0000358.pd

Situation Analysis and Needs Assessment Report for My Loi village and Ha Tinh province, Viet Nam

My Loi village is located in the uplands of Ky Son commune, Ky Anh district, Ha Tinh province on the north central coast of Viet Nam. In 2014, it was chosen as a site for Climate-Smart Village because of its exposure to multiple extreme weather events (temperature and water stress, storm and typhoon) and the potential for climate-smart solutions. The purpose of situation analysis and needs assessment was to understand the current situation at the village and province levels, on a number of issues, including food security and natural resources management, and to identify and prioritize the needs for My Loi to develop agriculture and livelihoods in synergy with climate adaptation and mitigation interventions. Data collection was conducted in October 2014 alongside a Village Baseline Study. The findings were shared during a feedback meeting in December 2014. The major findings for My Loi Village included: 1) main livelihood sources are in forestry (140 ha acacia and eucalyptus planted in near 200 ha) and rainfed agriculture (55 ha, paddy rice, peanut, maize, green bean, and sweet potato); 2) main constraints for production are water scarcity and poor soil quality; 3) livestock was promoted to improve livelihood diversification; Low investment capital and diseases are the households’ main challenges for expansion from an average of 1-2 cows per household. Larger herds could open up opportunities for biogas production. Feed sources, however, are unclear. The villagers identified 21 stakeholders active within food security (the majority), food crisis and natural resource management. The village experienced temporary periods of food insecurity during natural disasters. While the main issues relating to natural resources were pollution (mining) and land degradation, there was little awareness of the potential impacts due to progressing climate variability and change. The CCAFS CSV projects, therefore, are a timely complement to help implement the province action plan and policies in response to climate change

Isolation and characterization of the xenopus laevis cdna and genomic homologs of neuropeptide Y

We have isolated Xenopus laevis cDNA and genomic clones encoding the neuropeptide Y (NPY) mRNA and gene using a probe from the human NPY gene. The longest open reading frame in the cDNA encodes a peptide 76% identical to human prepro-NPY and 73% identical to rat prepro-NPY. The putative mature Xenopus NPY (XNPY) peptide is 94% identical to both human and rat peptides. A genomic clone containing 422 base pairs of 5'-flanking sequences and the 5'-end of the mRNA was also isolated. Primer extension analysis was used to map the transcription initiation site of the Xenopus NPY gene. Comparison of the 5 '-flanking sequences of the Xenopus laevis, human, and rat NPY genes resulted in areas of high conservation, including the TATA box and the CT box previously shown to interact with Spl-like proteins. Distribution of the Xenopus NPY message was analyzed by Northern analysis and RNAse protection. XNPY transcripts were not detected in whole developing embryo RNA, but were detected in adult frog brain RNA. We have also conducted preliminary studies of the XNPY promoter, utilizing an XNPY/chloramphenicol acetyl-transferase fusion construct. This study has demonstrated that Xenopus NPY shares a high degree of identity to its human and rat counterparts and that this homology extends to the gene, which contains similar cis-elements positioned near the transcription start site.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/31586/1/0000515.pd

Identification of Novel Methylation Markers in Hepatocellular Carcinoma using a Methylation Array

Promoter CpG island hypermethylation has become recognized as an important mechanism for inactivating tumor suppressor genes or tumor-related genes in human cancers of various tissues. Gene inactivation in association with promoter CpG island hypermethylation has been reported to be four times more frequent than genetic changes in human colorectal cancers. Hepatocellular carcinoma is also one of the human cancer types in which aberrant promoter CpG island hypermethylation is frequently found. However, the number of genes identified to date as hypermethylated for hepatocellular carcinoma (HCC) is fewer than that for colorectal cancer or gastric cancer, which can be attributed to fewer attempts to perform genome-wide methylation profiling for HCC. In the present study, we used bead-array technology and coupled methylation-specific PCR to identify new genes showing cancer-specific methylation in HCC. Twenty-four new genes have been identified as hypermethylated at their promoter CpG island loci in a cancer-specific manner. Of these, TNFRSF10C, HOXA9, NPY, and IRF5 were frequently hypermethylated in hepatocellular carcinoma tissue samples and their methylation was found to be closely associated with inactivation of gene expression. Further study will be required to elucidate the clinicopathological implications of these newly found DNA methylation markers in hepatocellular carcinoma

DNA methylation in the promoter region of the p16 (CDKN2/MTS-1/INK4A) gene in human breast tumours

The p16 (CDKN2/MTS-1/INK4A) gene is one of several tumour-suppressor genes that have been shown to be inactivated by DNA methylation in various human cancers including breast tumours. We have used bisulphite genomic sequencing to examine the detailed sequence specificity of DNA methylation in the CpG island promoter/exon 1 region in the p16 gene in DNA from a series of human breast cancer specimens and normal human breast tissue (from reductive mammaplasty). The p16 region examined was unmethylated in the four normal human breast specimens and in four out of nine breast tumours. In the other five independent breast tumour specimens, a uniform pattern of DNA methylation was observed. Of the nine major sites of DNA methylation in the amplified region from these tumour DNAs, four were in non-CG sequences. This unusual concentration of non-CG methylation sites was not a general phenomenon present throughout the genome of these tumour cells because the methylated CpG island regions of interspersed L1 repeats had a pattern of (almost exclusively) CG methylation similar to that found in normal breast tissue DNA and in DNA from tumours with unmethylated p16 genes. These data suggest that DNA methylation of the p16 gene in some breast tumours could be the result of an active process that generates a discrete methylation pattern and, hence, could ultimately be amenable to theraputic manipulation. © 1999 Cancer Research Campaig

Transcriptome Analysis of the Desert Locust Central Nervous System: Production and Annotation of a Schistocerca gregaria EST Database

) displays a fascinating type of phenotypic plasticity, designated as ‘phase polyphenism’. Depending on environmental conditions, one genome can be translated into two highly divergent phenotypes, termed the solitarious and gregarious (swarming) phase. Although many of the underlying molecular events remain elusive, the central nervous system (CNS) is expected to play a crucial role in the phase transition process. Locusts have also proven to be interesting model organisms in a physiological and neurobiological research context. However, molecular studies in locusts are hampered by the fact that genome/transcriptome sequence information available for this branch of insects is still limited. EST information is highly complementary to the existing orthopteran transcriptomic data. Since many novel transcripts encode neuronal signaling and signal transduction components, this paper includes an overview of these sequences. Furthermore, several transcripts being differentially represented in solitarious and gregarious locusts were retrieved from this EST database. The findings highlight the involvement of the CNS in the phase transition process and indicate that this novel annotated database may also add to the emerging knowledge of concomitant neuronal signaling and neuroplasticity events. EST data constitute an important new source of information that will be instrumental in further unraveling the molecular principles of phase polyphenism, in further establishing locusts as valuable research model organisms and in molecular evolutionary and comparative entomology

Biochemistry and physiology of gastrointestinal somatostatin

Somatostatin, a tetradecapeptide initially isolated from the ovine hypothalamus, is widely distributed throughout the gastrointestinal tract where it may act as a hormone, local chemical messenger, or neurotransmitter to elicit many physiological actions. Release of somatostatin from D cells in the gut is regulated by mechanisms that are both dependent on and independent of cAMP. In most cases somatostatin acts to inhibit the function of its target cells. It performs this action in part via pertussis-toxin-sensitive inhibitory guanine nucleotide-binding proteins that regulate adenylate cyclase activity. Other mechanisms may involve sites of action distal to intracellular second messenger systems .Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44411/1/10620_2005_Article_BF01536041.pd