12 research outputs found
Augmenter la vitesse dâinjection de la cocaĂŻne favorise lâapparition de comportements de consommation caractĂ©ristiques de la toxicomanie
Nombreux individus vont expĂ©rimenter avec les drogues dâabus, mais peu vont devenir toxicomanes. Plusieurs facteurs sont impliquĂ©s dans la transition dâun usage rĂ©crĂ©atif Ă lâaddiction. Les drogues, les conditionnements et les voies dâadministration qui mĂšnent Ă lâaugmentation rapide du taux drogue dans le cerveau favorisent cette Ă©volution. La raison est mĂ©connue. Nous avons Ă©mis lâhypothĂšse que lâinjection rapide de drogue promeut des changements dans le cerveau qui mĂšnent Ă lâaugmentation de la consommation et de la motivation Ă obtenir la drogue. Nous avons comparĂ© la consommation lors de conditions Ă ratio fixe (FR) et Ă ratio progressif (PR) chez des rats sâauto-administrant la cocaĂŻne administrĂ©e par voie intraveineuse (i.v.) en 5 ou 90 secondes (s). Tous les rats ont Ă©tĂ© entrainĂ©s Ă peser sur un levier afin de sâauto administrer des injections de cocaĂŻne de 0.25 ou 0.5 mg/kg par voie intraveineuse injectĂ©e en 5 s sous FR avant dâĂȘtre divisĂ©s en groupes sâauto administrant la cocaĂŻne injectĂ©e en 5 ou 90 s pendant 1 heure (h)/session. Pour Ă©tudier les diffĂ©rences potentielles en consommation, lâaccĂšs Ă la cocaĂŻne Ă Ă©tĂ© augmentĂ© Ă 6 h/session. Les diffĂ©rences en motivation ont Ă©tĂ© dĂ©tectĂ©es par lâauto administration de la cocaĂŻne sous PR en fonction de la dose et de la vitesse dâinfusion. LâaccĂšs Ă la drogue pendant 1 h/session nâa pas influencĂ© la consommation. Lorsque lâaccĂšs a Ă©tĂ© prolongĂ© Ă 6 h, tous les animaux ont augmentĂ© leur consommation, mais lâaugmentation Ă©tait plus prononcĂ©e chez les rats sâinjectant la cocaĂŻne en 5 s. De plus, la vitesse dâinjection a influencĂ© la motivation pour obtenir la drogue. Lors de conditions Ă PR, la courbe dose-rĂ©ponse pour le nombre dâinfusions prises a Ă©tĂ© dĂ©placĂ©e vers le haut pour les rats sâauto administrant des injections de cocaĂŻne en 5 s versus 90 s. De plus, des diffĂ©rences qualitatives on Ă©tĂ© observĂ©es en PR. La consommation de cocaĂŻne des rats sâinjectant des infusions en 5 s Ă©tait dĂ©pendante de la dose, tandis que les rats sâauto administrant la drogue en 90 s ont pris la mĂȘme quantitĂ© de drogue, peu importe la dose. Finalement, les rats sâauto administrant des infusions de cocaĂŻne 0.5 mg/kg en 5 s ont consommĂ© plus de cocaĂŻne que les rats prenant des infusions en 90 s, peu importe si elle Ă©tait injectĂ©e en 5 ou 90 s le jour du test. Ainsi, nos rĂ©sultats montrent que lâinjection rapide de drogue dans le cerveau mĂšne Ă lâaugmentation de la consommation et de la motivation pour obtenir la cocaĂŻne, deux symptĂŽmes qui caractĂ©risent la toxicomanie.While many people will experiment with drugs of abuse, few will become addicts. Many factors have been implicated in the transition from recreational drug use to addiction. Drugs, formulations and routes of administration that lead to the rapid rise of drug levels in the brain are thought to facilitate this evolution. The reason for this remains unknown. We hypothesized that the rapid delivery of drugs might promote certain changes in the brain leading to increased drug intake and greater motivation to obtain the drug. In order to assess the effects of the speed of administration, we compared drug intake under fixed (FR) and progressive (PR) ratio conditions in rats self-administering intravenous (i.v.) cocaine injections delivered over either 5 or 90 seconds (s). Rats were trained to press a lever for 0.25 or 0.5 mg/kg cocaine injections delivered over 5 s under a FR schedule of reinforcement, before being divided into groups self-administering cocaine delivered over either 5 or 90 s for 1 hour (h)/session. To assess potential differences in drug consumption, access to cocaine was increased to 6 h/session. To assess differences in motivation for cocaine, drug self-administration was determined under a PR schedule of reinforcement both as a function of dose and infusion rate. When animals were given access to i.v. cocaine for 1 h/session, the infusion speed did not influence drug consumption. However, when access to the drug was prolonged to 6 h/session, all animals augmented their drug intake, though the increase was greater in animals self-administering the drug delivered more rapidly (over 5 vs. 90 s). The speed of drug delivery also influenced the motivation for cocaine. Under PR conditions, the dose response curve for the number of self-administered infusions was shifted upward in the 5-s animals relative to those in the 90-s group. Moreover, qualitative differences were observed in cocaine intake under PR conditions. Whereas the intake of rats self-administering cocaine delivered over 5 s was dose-dependent, drug consumption in rats injecting the drug over 90 s did not vary with the dose. Finally, rats self-administering 0.5 mg/kg cocaine infusions delivered over 5 s took more cocaine than the rats receiving it over 90 s, regardless of whether cocaine was delivered over 5 or 90 s during PR testing. Thus, our results show that increasing the speed at which cocaine is delivered to the brain leads to greater drug intake and increased willingness to expend effort to obtain the drug, two important symptoms of addiction
Augmenter la vitesse dâinfusion de la cocaĂŻne par voie intraveineuse induit des changements neurochimiques, neurobiologiques et comportementaux chez le rat : implications pour la toxicomanie
Plusieurs individus consomment la cocaĂŻne, mais peu dĂ©veloppent des troubles liĂ©s Ă lâusage de la drogue. Les facteurs pharmacocinĂ©tiques de la drogue et le patron de consommation sont importants dans ce processus. Les drogues et les voies dâadministration qui produisent lâaugmentation rapide des niveaux de drogue dans le cerveau faciliteraient cette transition. Similairement, lâaccĂšs intermittent Ă la cocaĂŻne, qui induit des fluctuations de drogue dans le cerveau, serait impliquĂ© aussi. Le travail prĂ©sentĂ© ici regarde de prĂšs comment la vitesse dâadministration et la frĂ©quence de consommation induisent des comportements et de la neuroplasticitĂ© caractĂ©ristiques Ă la toxicomanie chez le rat. Dans une premiĂšre Ă©tude, nous avions dĂ©terminĂ© les effets de la cocaĂŻne sur les niveaux de drogue et de dopamine dans le striatum dorsal par microdialyse. La gamme de vitesses utilisĂ©e (5-90 secondes (s)) nâa pas modifiĂ© la concentration maximale de ces deux molĂ©cules, mais les infusions rapides ont fait que ces concentrations maximales soient atteintes plus rapidement. Ainsi, les diffĂ©rences induites par la vitesse dâadministration sur le comportement et la neuroplasticitĂ© seraient dues Ă la vitesse Ă laquelle la drogue arrive au cerveau, et non Ă la quantitĂ© de drogue quây parvient. Nos travaux prĂ©cĂ©dents montrent que les infusions rapides de cocaĂŻne augmentent la motivation pour la drogue. Afin de mieux comprendre les mĂ©canismes qui sous-tendent cette augmentation, nous avions examinĂ© la neuroplasticitĂ© induite par lâinfusion rapide de cocaĂŻne chez des animaux montrant une motivation excessive pour la drogue sous ratio progressif. PremiĂšrement, nous avions Ă©tudiĂ© les effets de la vitesse dâinfusion sur lâexpression corticostriatale de lâARN messager (ARNm) du brain-derived neurotrophic factor (BDNF) et TrkB, son rĂ©cepteur, chez des rats ayant un historique de consommation en continu (6 heures/session). Seulement les rats sâinjectant des infusions rapides de drogue (infusĂ©es en 5 s) ont montrĂ© des altĂ©rations dans les niveaux de BDNF et TrkB, telles que lâaugmentation des niveaux de lâARNm de BDNF dans les cortex orbitofrontal, frontal et pariĂ©tal et la baisse des niveaux de lâARNm de TrkB dans les cortex cingulĂ©, frontal et pariĂ©tal et le striatum dorsal versus les rats sâauto-administrant des infusions plus lentes (infusĂ©es en 90 s). Ensuite, nous avions utilisĂ© un modĂšle dâaccĂšs intermittent, oĂč la concentration de cocaĂŻne dans le cerveau fluctue durant la session, pour dĂ©terminer les effets de la vitesse dâinfusion sur lâexpression de lâARN messager c-fos, un marqueur dâactivitĂ© neuronale. Il a Ă©tĂ© rĂ©cemment reportĂ© dans la littĂ©rature que les consommateurs de cocaĂŻne expĂ©rimentĂ©s ne consomment pas de façon continue, mais prĂ©fĂšrent espacer leur consommation, donc un modĂšle dâaccĂšs intermittent serait plus appropriĂ© pour lâĂ©tude de la toxicomanie. Ainsi, un premier groupe dâanimaux a eu un accĂšs intermittent Ă des infusions rapides de cocaĂŻne, un modĂšle considĂ©rĂ© pertinent Ă la toxicomanie, tandis que lâautre a eu accĂšs Ă des infusions lentes en continu, qui permet une consommation importante sans provoquer des symptĂŽmes caractĂ©ristiques Ă la toxicomanie. Le groupe pertinent Ă la toxicomanie Ă©tait plus motivĂ© pour la drogue. De plus, ce groupe a montrĂ© une augmentation de lâexpression de c-fos dans plusieurs structures corticostriatales. Ces rĂ©sultats suggĂšrent que les infusions rapides et intermittentes de cocaĂŻne facilitent le dĂ©veloppement de neuroplasticitĂ© dans le cerveau, qui peut ensuite changer le comportement. De plus, le cortex orbitofrontal et le striatum dorsal seraient potentiellement impliquĂ©s. Ces deux structures sont interconnectĂ©es et sont engagĂ©es dans la toxicomanie. Nous avions dĂ©connectĂ© temporairement et pharmacologiquement ce circuit et avions observĂ© une baisse significative de la motivation pour la drogue. En conclusion, les facteurs pharmacocinĂ©tiques sont importants dans le dĂ©veloppement de modĂšles animaux adĂ©quats de la toxicomanie. Une meilleure comprĂ©hension des mĂ©canismes impliquĂ©s pourrait Ă©ventuellement amĂ©liorer le dĂ©veloppement de pharmacothĂ©rapies pour la prĂ©vention et le traitement de la toxicomanie.Many individuals will experiment with cocaine during their lifetime; yet, few will transition from occasional drug use to addiction. Pharmacokinetic factors of the drug and the way it is consumed are important in this process. Drugs and routes of administration that produce the rapid rise of drug levels in the brain are believed to facilitate this transition. Similarly, intermittent access to the drug, which provokes fluctuations of cocaine concentrations in the brain rather than maintaining continuous high levels, may also be involved. The work presented in this thesis examines how speed of drug delivery and frequency of intake may induce addiction-like symptoms in a rat model of cocaine self-administration and promote neuroplasticity. In a first study, microdialysis was used to determine the effects of rate of cocaine infusion on cocaine and dopamine levels in the dorsal striatum of awake, freely-moving rats. Over the range of infusion rates used (5-90 seconds), cocaine and dopamine concentrations did not significantly vary. Instead, rapid cocaine infusions lead to the faster rate of onset of both molecules in the brain, implying that differences in cocaine-induced behaviour and neuroplasticity are not due to how much drug reaches the brain but on how fast the drug does so. Previous work from our laboratory showed that rapid cocaine infusions increase motivation for cocaine, a symptom of addiction. Therefore, we examined potential changes in the brain caused by rapid cocaine infusions in animals showing excessive motivation for the drug as assessed under a progressive ratio schedule of reinforcement. First, we investigated the effects of cocaine infusion rate on corticostriatal expression of brain-derived neurotrophic factor (BDNF) and TrkB mRNA in rats with a history of chronic and continuous exposure to cocaine under long access conditions (6 hours/session) and found that only rats self-administering rapid cocaine injections had altered BDNF and TrkB mRNA levels. Next, new evidence suggested that experienced cocaine users do not consume the drug continuously, but rather space out each intoxicating event. Therefore, using an intermittent access model of self-administration, where brain cocaine levels fluctuate within the session, we examined the effects of rate of infusion on c-fos mRNA expression, a marker of neuronal activity. A first group of rats had intermittent access to rapid cocaine infusions, a model we consider as addiction-relevant, while the second had access to slow and continuous cocaine, which promotes significant cocaine intake without promoting addiction-like behaviour. Animals in the addiction-relevant group presented greater motivation for cocaine and showed greater c-fos mRNA expression in various corticostriatal regions. Together, these results suggest that rapid and intermittent cocaine infusions facilitate the development of drug-induced neuroplasticity which may cause addiction-like behaviour, and particularly implicate the orbitofrontal cortex and the dorsal striatum. These two interconnected structures are both involved in addiction. Therefore, we temporarily and pharmacologically disconnected this circuit and found that it reduces motivation for cocaine. In conclusion, we show that pharmacokinetic factors are important when developing animal models of addiction, and that better understanding the mechanisms involved can potentially improve prevention and treatment therapies
How fast and how often: the pharmacokinetics of drug use are decisive in addiction
How much, how often and how fast a drug reaches the brain determine the behavioural and neuroplastic changes associated with the
addiction process. Despite the critical nature of these variables, the drug addiction field often ignores pharmacokinetic issues, which
we argue can lead to false conclusions. First, we review the clinical data demonstrating the importance of the speed of drug onset and
of intermittent patterns of drug intake in psychostimulant drug addiction. This is followed by a review of the preclinical literature
demonstrating that pharmacokinetic variables play a decisive role in determining behavioural and neurobiological outcomes in animal
models of addiction. This literature includes recent data highlighting the importance of intermittent, âspikingâ brain levels of drug in
producing an increase in the motivation to take drug over time. Rapid drug onset and intermittent drug exposure both appear to push
the addiction process forward most effectively. This has significant implications for refining animal models of addiction and for better
understanding the neuroadaptations that are critical for the disorder
Varying the rate of intravenous cocaine infusion influences the temporal dynamics of both drug and dopamine concentrations in the striatum
The faster drugs of abuse reach the brain, the greater is the risk of addiction. Even small differences in the rate of drug delivery can influence outcome. Infusing cocaine intravenously over 5 vs. 90Ăą 100ĂÂ s promotes sensitization to the psychomotor and incentive motivational effects of the drug and preferentially recruits mesocorticolimbic regions. It remains unclear whether these effects are due to differences in how fast and/or how much drug reaches the brain. Here, we predicted that varying the rate of intravenous cocaine infusion between 5 and 90ĂÂ s produces different rates of rise of brain drug concentrations, while producing similar peak concentrations. Freely moving male Wistar rats received acute intravenous cocaine infusions (2.0ĂÂ mg/kg/infusion) over 5, 45 and 90ĂÂ s. We measured cocaine concentrations in the dorsal striatum using rapidĂą sampling microdialysis (1ĂÂ sample/min) and highĂą performance liquid chromatographyĂą tandem mass spectrometry. We also measured extracellular concentrations of dopamine and other neurochemicals. Regardless of infusion rate, acute cocaine did not change concentrations of nonĂą dopaminergic neurochemicals. Infusion rate did not significantly influence peak concentrations of cocaine or dopamine, but concentrations increased faster following 5Ăą s infusions. We also assessed psychomotor activity as a function of cocaine infusion rate. Infusion rate did not significantly influence total locomotion, but locomotion increased earlier following 5Ăą s infusions. Thus, small differences in the rate of cocaine delivery influence both the rate of rise of drug and dopamine concentrations, and psychomotor activity. A faster rate of rise of drug and dopamine concentrations might be an important issue in making rapidly delivered cocaine more addictive.Varying the rate of i.v. cocaine delivery between 5 and 90ĂÂ s determines the drugâs effects on brain and behaviour. We show that injecting cocaine between 5 and 90ĂÂ s in rats alters the rates of rise of cocaine and dopamine in the dorsal striatum, without significantly changing peak concentrations. Faster injections also increase locomotor behaviour earlier than slower injections. Thus, beyond achieved dose, differences in the rates of rise of cocaine and dopamine can determine outcome.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/151808/1/ejn13941-sup-0002-reviewer-Comments.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/151808/2/ejn13941.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/151808/3/ejn13941-sup-0001-FigS1-S3.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/151808/4/ejn13941_am.pd
Cues paired with either rapid or slower self-administered cocaine injections acquire similar conditioned rewarding properties.
The faster drugs of abuse reach the brain, the more addictive they can be. It is not known why this is. Environmental stimuli associated with drugs can promote the development and persistence of addiction by invigorating and precipitating drug-seeking behaviour. We determined, therefore, whether cues associated with the self-administration of rapidly delivered cocaine (injected intravenously over 5 versus 90 seconds) would acquire greater conditioned rewarding properties, as assessed by the performance of an operant response reinforced solely by the cues. Rats nose-poked for intravenous cocaine infusions delivered either over 5 or 90 seconds. Discrete visual cues accompanied each infusion. The rats could then press a lever to obtain the cues--now a conditioned reward--or an inactive lever. Rats in both the 5- and 90-second groups pressed more on the active versus inactive lever following extensive (24 sessions) but not following limited (3 sessions) self-administration training. There were no group differences in this behaviour. Following withdrawal from cocaine self-administration, lever discrimination progressively abated in both groups and was lost by withdrawal day 30. However, the rewarding properties of the cues were not "forgotten" because on withdrawal days 32-33, amphetamine selectively enhanced active-lever pressing, and did so to a similar extent in both groups. Thus, cues paired with rapid or slower cocaine delivery acquire similar conditioned rewarding properties. We conclude, therefore, that the rapid delivery of cocaine to the brain promotes addiction by mechanisms that might not involve a greater ability of drug cues to control behaviour
The self-administration of rapidly delivered cocaine promotes increased motivation to take the drug: contributions of prior levels of operant responding and cocaine intake
Abstract Rationale Rapid drug delivery to the brain might increase the risk for developing addiction. In rats, increasing the speed of intravenous cocaine delivery (5 vs. 90 s) increases drug intake and the subsequent motivation to self-administer cocaine. Increased motivation for cocaine could result not only from more extensive prior drug intake and operant responding for drug, but also from neuroplasticity evoked by rapid drug uptake. Objective We determined the contributions of prior drug intake and operant responding to the increased motivation for cocaine evoked by rapid delivery. We also investigated the effects of cocaine delivery speed on corticostriatal expression of brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrkB) mRNA. Methods Rats self-administered cocaine (0.25 mg/kg/infusion) delivered over 5 or 90 s during short-access (1 h/session; ShA) or long-access (6 h; LgA) sessions. Motivation for cocaine was then assessed by measuring responding under a progressive ratio schedule of reinforcement. Next, BDNF and TrkB mRNA levels were measured in 5-and 90-s rats. Results Five-second ShA and 5-s-LgA rats were more motivated for cocaine than their 90-s counterparts. This effect was dissociable from previous levels of drug intake or of operant responding for cocaine. In parallel, only rats selfadministering rapid cocaine injections had altered BDNF and TrkB mRNA levels in corticostriatal regions. Conclusions Rapid drug delivery augments the motivation for cocaine independently of effects on the levels of drug intake or operant responding for drug. We suggest that rapid delivery might increase the motivation for drug by promoting neuroplasticity within reward pathways. This neuroplasticity could involve increased regulation of BDNF/TrkB
Cues Paired with either Rapid or Slower Self-Administered Cocaine Injections Acquire Similar Conditioned Rewarding Properties
MinogianisDataFiles.xlsx
<p><a>The faster drugs of abuse reach the brain,
the greater is the risk of addiction. Even small differences in the rate of
drug delivery can influence outcome. Infusing cocaine intravenously over 5
versus 90-100 seconds promotes sensitization to the psychomotor and incentive
motivational effects of the drug and preferentially recruits mesocorticolimbic
regions. It remains unclear whether these effects are due to differences in how
fast and/or how much drug reaches the brain.</a> <a>Here, we predicted that varying the rate of intravenous cocaine
infusion between 5-90 seconds produces different rates of rise of brain drug concentrations,
while producing similar peak concentrations. Freely-moving male Wistar rats received
acute intravenous cocaine infusions (2.0 mg/kg/infusion) over 5, 45 and 90 seconds.
</a><a>We measured cocaine concentrations in the dorsal
striatum using rapid-sampling microdialysis (1 sample/minute) and high-performance
liquid chromatography-tandem mass spectrometry. We also measured extracellular concentrations
of dopamine and other neurochemicals. Regardless of infusion rate, acute
cocaine did not change concentrations of non-dopaminergic neurochemicals. Infusion
rate did not significantly influence peak concentrations of cocaine or dopamine,
but concentrations increased faster following 5-second infusions. </a><a>We also assessed psychomotor activity as a function of
cocaine infusion rate. Infusion rate did not significantly influence total locomotion,
but locomotion increased earlier following 5-second infusions. Thus, small
differences in the rate of cocaine delivery influence both the rate of rise of
drug and dopamine concentrations and psychomotor activity. A faster rate of rise
of drug and dopamine concentrations might be an important issue in making
rapidly delivered cocaine more addictive.</a></p
Dataset for: Varying the rate of intravenous cocaine infusion influences the temporal dynamics of both drug and dopamine concentrations in the striatum
The faster drugs of abuse reach the brain, the greater is the risk of addiction. Even small differences in the rate of drug delivery can influence outcome. Infusing cocaine intravenously over 5 versus 90-100 seconds promotes sensitization to the psychomotor and incentive motivational effects of the drug and preferentially recruits mesocorticolimbic regions. It remains unclear whether these effects are due to differences in how fast and/or how much drug reaches the brain. Here, we predicted that varying the rate of intravenous cocaine infusion between 5-90 seconds produces different rates of rise of brain drug concentrations, while producing similar peak concentrations. Freely-moving male Wistar rats received acute intravenous cocaine infusions (2.0 mg/kg/infusion) over 5, 45 and 90 seconds. We measured cocaine concentrations in the dorsal striatum using rapid-sampling microdialysis (1 sample/minute) and high-performance liquid chromatography-tandem mass spectrometry. We also measured extracellular concentrations of dopamine and other neurochemicals. Regardless of infusion rate, acute cocaine did not change concentrations of non-dopaminergic neurochemicals. Infusion rate did not significantly influence peak concentrations of cocaine or dopamine, but concentrations increased faster following 5-second infusions. We also assessed psychomotor activity as a function of cocaine infusion rate. Infusion rate did not significantly influence total locomotion, but locomotion increased earlier following 5-second infusions. Thus, small differences in the rate of cocaine delivery influence both the rate of rise of drug and dopamine concentrations and psychomotor activity. A faster rate of rise of drug and dopamine concentrations might be an important issue in making rapidly delivered cocaine more addictive