12 research outputs found
IL-1β promotes ADAMTS enzyme-mediated aggrecan degradation through NF-κB in human intervertebral disc
IL-1β promotes ADAMTS enzyme-mediated aggrecan degradation through NF-κB in human intervertebral disc
Property Prediction of Ag-Filled Isotropic Conductive Adhesive through the Analysis of Its Curing and Decomposition Kinetics
In this study, various thermal analyses were carried out on a self-developed and commerce-oriented Ag-filled isotropic conductive adhesive (ICA) and its unfilled matrix resin through which glass transition temperature (Tg) and thermal endurance could be quantitatively predicted. An autocatalyzed kinetic model was used to describe the curing reaction, which was proven to be in good consistency with the experimental data. The activation energies for the curing reaction of the ICA and the matrix resin were determined to be 68.1 kJ/mol and 72.9 kJ/mol, respectively, which means that the reaction of the ICA was easier to occur than its unfilled matrix resin. As a result, the time–temperature profile could be calculated for any Tg requested based on the kinetic model of curing and the DiBenedetto equation. Further, the thermal decomposition stability of the ICA and its unfilled matrix resin were also studied. The activation energies for the thermal decomposition of the ICA and the matrix resin were calculated to be 134.1 kJ/mol and 152.7 kJ/mol, respectively, using the Ozawa–Flynn–Wall method, which means that the decomposition of ICA was easier to occur. The service life of the resin system at a specific temperature could therefore be calculated with their activation energy. The addition of micro-scale Ag flakes did not change the curing and decomposition mechanisms by much
Property Prediction of Ag-Filled Isotropic Conductive Adhesive through the Analysis of Its Curing and Decomposition Kinetics
In this study, various thermal analyses were carried out on a self-developed and commerce-oriented Ag-filled isotropic conductive adhesive (ICA) and its unfilled matrix resin through which glass transition temperature (Tg) and thermal endurance could be quantitatively predicted. An autocatalyzed kinetic model was used to describe the curing reaction, which was proven to be in good consistency with the experimental data. The activation energies for the curing reaction of the ICA and the matrix resin were determined to be 68.1 kJ/mol and 72.9 kJ/mol, respectively, which means that the reaction of the ICA was easier to occur than its unfilled matrix resin. As a result, the time–temperature profile could be calculated for any Tg requested based on the kinetic model of curing and the DiBenedetto equation. Further, the thermal decomposition stability of the ICA and its unfilled matrix resin were also studied. The activation energies for the thermal decomposition of the ICA and the matrix resin were calculated to be 134.1 kJ/mol and 152.7 kJ/mol, respectively, using the Ozawa–Flynn–Wall method, which means that the decomposition of ICA was easier to occur. The service life of the resin system at a specific temperature could therefore be calculated with their activation energy. The addition of micro-scale Ag flakes did not change the curing and decomposition mechanisms by much
The influence of different cutting parameters on the glass edge quality
The influence of different cutting parameters on the glass edge quality was investigated, including the cutter material, the sharpening angle of the cutting roll, the cutting speed and the load applied to the roll. The results show that there are less defects on the edge of the glass cut by diamond cutter. There is no obvious influence of cutting speed on the glass edge quality. The cutter with a smaller sharpening angle is more applicable for the cutting of thin glass, and the thick glass is more suitable to use a bigger sharpening angle cutter. Higher cutting load is helpful for the breaking of the glass along the cutting line. However, it may cause more defects on the edge and the surface of the glass
Positive Enrichment of C‑Terminal Peptides Using Oxazolone Chemistry and Biotinylation
Selective
capture of protein C-termini is still challenging in
view of the lower reactivity of the carboxyl group relative to amino
groups and difficulties in site-specifically labeling the carboxyl
group on the C-terminus rather than that on the side chains of acidic
amino acids. For highly efficient purification of C-terminus peptides,
a novel positive enrichment approach based on the oxazolone chemistry
has been developed in this study. A bifunctional group reagent containing
biotin and arginine was incorporated into the C-terminus of protein.
Together with a streptavidin affinity strategy, the C-terminal peptides
could be readily purified and analyzed by mass spectrometry (MS).
Unlike the negative enrichment approach, C-terminal peptides, other
than non-C-terminal peptides, were captured directly from the peptide
mixture in this new method. The labeling efficiency (higher than 90%),
enrichment selectivity (purifying C-terminal peptides from mixtures
of non-C-terminal peptides at a 1:50 molar ratio), and ionization
efficiencies in MS were dramatically improved. Moreover, the highly
efficient identification of C-terminal peptides was further achieved
by defining biotin as the 21st amino acid and optimizing the database
search strategy. We have successfully identified 183 C-terminal peptides
from Thermoanaerobacter tengcongensis using this creative method, which affords a highly selective and
efficient purification approach for C-terminomics study