Compressive sensing based velocity estimation in video data

This paper considers the use of compressive sensing based algorithms for velocity estimation of moving vehicles. The procedure is based on sparse reconstruction algorithms combined with time-frequency analysis applied to video data. This algorithm provides an accurate estimation of object's velocity even in the case of a very reduced number of available video frames. The influence of crucial parameters is analysed for different types of moving vehicles.Comment: 4 pages, 5 figure

Onco-miR-155 targets SHIP1 to promote TNFalpha-dependent growth of B cell lymphomas.

Non-coding microRNAs (miRs) are a vital component of post-transcriptional modulation of protein expression and, like coding mRNAs harbour oncogenic properties. However, the mechanisms governing miR expression and the identity of the affected transcripts remain poorly understood. Here we identify the inositol phosphatase SHIP1 as a bonafide target of the oncogenic miR-155. We demonstrate that in diffuse large B cell lymphoma (DLBCL) elevated levels of miR-155, and consequent diminished SHIP1 expression are the result of autocrine stimulation by the pro-inflammatory cytokine tumour necrosis factor a (TNFalpha). Anti-TNFalpha regimen such as eternacept or infliximab were sufficient to reduce miR-155 levels and restored SHIP1 expression in DLBCL cells with an accompanying reduction in cell proliferation. Furthermore, we observed a substantial decrease in tumour burden in DLBCL xenografts in response to eternacept. These findings strongly support the concept that cytokine-regulated miRs can function as a crucial link between inflammation and cancer, and illustrate the feasibility of anti-TNFalpha therapy as a novel and immediately accessible (co)treatment for DLBCL

DINAMIKA UČENJA I IZVOĐENJA GIMNASTIČKIH ELEMENATA U TJELESNOJ I ZDRAVSTVENOJ KULTURI – RAZLIKE PO SPOLU

Gender differences in acquiring and retaining gymnastic skills in first graders were studied. Forty-four (44) girls and thirty-two (32) boys (mean age 6.5 years) participated as the experimental group. They were included in the 35-week-long program based on four gymnastic skills. Their performance was measured in three acquisition and two retention phases. The results showed that the girls performed better than the boys. The independent sample t-test showed significant gender differences in the majority of measurements. ANOVA for the retention phases revealed a positive learning transfer only in case of the candlestick element for the boys and in case of the cartwheel element for the girls. The differences found are not considered as a disturbing factor for the coeducational teaching method but just as the guidelines for the PE teachers when making their curricula more appropriate and individually approached.Istraživane su razlike po spolu u dinamici učenja i retenciji gimnastičkih znanja na uzorku učenika prvog razreda osnovne škole. Eksperimentalnu grupu činilo je 44 učenice i 32 učenika koji su sudjelovali u tretmanu u trajanju od 35 tjedana, a koji je uključivao i četiri gimnastička znanja. Izvođenje tih znanja procjenjivano je u 3 faze učenja i 2 retencijske faze. Govoreći o usvajanju gimnastičkih znanja, rezultati su ukazali na bolje izvođenje djevojčica za razliku od dječaka. T test za nezavisne uzorke potvrdio je značajnu razliku između spolova u većini točaka provjeravanja. Rezultati ANOVA testa za retencijske točke provjeravanja ukazali su na pozitivan transfer učenja samo za element stava na lopaticama kod dječaka i premeta strance kod djevojčica. Pronađene razlike između spolova ne smatraju se remetećim faktorom za postojanje zajedničke metode poučavanja za oba spola, već primarno služe kao smjernice profesorima tjelesne i zdravstvene kulture za prilagođavanje nastavnog kurikuluma kako bi bio što prikladniji i usmjereniji na individualni pristup

Vav1/2/3-null mice define an essential role for vav family proteins in lymphocyte development and activation but a differential requirement in MAPK signaling in T and B cells

The Vav family of Rho guanine nucleotide exchange factors is thought to orchestrate signaling events downstream of lymphocyte antigen receptors. Elucidation of Vav function has been obscured thus far by the expression of three highly related family members. We generated mice lacking all Vav family proteins and show that Vav-null mice produce no functional T or B cells and completely fail to mount both T-dependent and T-independent humoral responses. Whereas T cell development is blocked at an early stage in the thymus, immature B lineage cells accumulate in the periphery but arrest at a late “transitional” stage. Mechanistically, we show that the Vav family is crucial for both TCR and B cell receptor (BCR)–induced Ca(2+) signaling and, surprisingly, is only required for mitogen-activated protein kinase (MAPK) activation in developing and mature T cells but not in B cells. Thus, the abundance of immature B cells generated in Vav-null mice may be due to intact Ras/MAPK signaling in this lineage. Although the expression of Vav1 alone is sufficient for normal lymphocyte development, our data also reveal lineage-specific roles for Vav2 and Vav3, with the first demonstration that Vav3 plays a critical compensatory function in T cells. Together, we define an essential role for the entire Vav protein family in lymphocyte development and activation and establish the limits of functional redundancy both within this family and between Vav and other Rho–guanine nucleotide exchange factors

Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals

This study explored humoral and cellular responses to anti-SARS-CoV-2 BNT162b2 mRNA vaccine in breastfeeding women and naïve and seropositive individuals in the first six months after vaccination.Sixty-one volunteers vaccinated with two doses of the BNT162b2 mRNA vaccine were enrolled in the study. In-house developed ELISA was used for the quantification of SARS-CoV-2 RBD-specific antibodies. Cell surface marker expression and intracellular IFN-γ analysis were carried out by flow cytometry. The concentrations of IFN-γ, IL-6 and TNF were determined by ELISA. A significant rise in anti-RBD IgG antibody levels was observed 14 days after the first vaccine dose (p < 0.0001) in serum and milk. The expression of CD28 on CD4+ T cells was significantly higher compared to baseline (p < 0.05). There was a significant increase (p ≤ 0.05) in B cell lymphocyte subset after revaccination, and increased percentage of CD80+ B cells. The expression of IFN-γ in peripheral blood lymphocytes, CD3+ T cells and serum was significantly increased (p < 0.05). No significant difference in immune response was observed between breastfeeding women and other study participants. The anti-SARS-CoV-2 BNT162b2 mRNA vaccine-induced measurable and durable immune response in breastfeeding women and in naïve and previously infected individuals

Effects of allergic diseases and age on the composition of serum IgG glycome in children

Acknowledgements Glycan analysis was partly supported by European Commission GlycoBioM (contract #259869), IBD-BIOM (contract #305479), HighGlycan (contract #278535), MIMOmics (contract #305280), HTP-GlycoMet (contract #324400) and IntegraLife (contract #315997) grants. The SEATON cohort was partly funded by the UK Medical Research Council (contract #80219) and Asthma UK (contract #00/011 and 02/017) grants.Peer reviewedPublisher PD

Evaluation of phenyl-propanedione on yellowing and chemical-mechanical properties of experimental dental resin-based materials

ABSTRACT Objective To evaluate the influence of phenyl-propanedione on yellowing and chemical-mechanical properties of experimental resin-based materials photoactivated using different light curing units (LCUs). Material and Methods Experimental resin-based materials with the same organic matrix (60:40 wt% BisGMA:TEGDMA) were mechanically blended using a centrifugal mixing device. To this blend, different photoinitiator systems were added in equimolar concentrations with aliphatic amine doubled by wt%: 0.4 wt% CQ; 0.38 wt% PPD; or 0.2 wt% CQ and 0.19 wt% PPD. The degree of conversion (DC), flexural strength (FS), Young&#8217;s modulus (YM), Knoop hardness (KNH), crosslinking density (CLD), and yellowing (Y) were evaluated (n=10). All samples were light cured with the following LCUs: a halogen lamp (XL 2500), a monowave LED (Radii), or a polywave LED (Valo) with 16 J/cm2. The results were analysed by two-way ANOVA and Tukey&#8217;s test (&#945;=0.05). Results No statistical differences were found between the different photoinitiator systems to KNH, CLS, FS, and YM properties (p&#8805;0.05). PPD/CQ association showed the higher DC values compared with CQ and PPD isolated systems when photoactivated by a polywave LED (p&#8804;0.05). Y values were highest for the CQ compared with the PPD systems (p&#8804;0.05). Conclusion PPD isolated system promoted similar chemical and mechanical properties and less yellowing compared with the CQ isolated system, regardless of the LCU used

Coordinate suppression of B cell lymphoma by PTEN and SHIP phosphatases

Mice lacking both PTEN and SHIP phosphatases develop spontaneous B cell lymphoma