An Orientation Program for Vertical Transfers in Engineering and Engineering Technology

This paper reports on a scholarship program funded by the National Science Foundation that focuses on students who transfer at the 3rd -year level from 2-year schools to the engineering and engineering technology BS programs at our university. The objectives of the program are to: (i) expand and diversify the engineering/technology workforce of the future, (ii) develop linkages and articulations with 2-year schools and their S-STEM (Scholarships in Science, Technology, Engineering and Mathematics) programs, (iii) provide increased career opportunities and job placement rates through mandatory paid co-op experiences, and (iv) serve as a model for other universities to provide vertical transfer students access to the baccalaureate degree. The program is in its third year. It recruited its first group of 25 students in Fall 2017, and another group of 27 students in Fall 2018. We hope to recruit 26 more students in Fall 2019 for a total of 78 vertical transfers. The goal is to retain and graduate at least 95% of these scholars. To enhance the success of these scholars, a zero-credit six-week orientation course was developed in Fall 2017 focusing on four dimensions of student wellness: academic, financial, social, and personal. This paper describes the development of this course, its content, and the modifications that were made to the course for Fall 2018. The paper will also address the research conducted in order to generate knowledge about the program elements that will be essential for the success of vertical transfer programs at other universities. Two research instruments are described: an online survey and a focus group interview that were developed, and administered to the transfer scholars in their first year. Initial findings concerning students’ experiences at their 2-year schools, their reason for transferring, their experience in transferring as well as their initial conceptions of what life at a 4-year institution will be like are presented

Scholarship Programs for Vertical Transfers in Engineering and Engineering Technology

This paper introduces two scholarship projects funded by the National Science Foundation that focus on students who transfer at the 3rd year level from 2-year schools to the engineering and engineering technology BS programs at our university. The objectives of both the projects are: (i) to expand and diversify the engineering/technology workforce of the future, (ii) to develop linkages and articulations with 2-year schools and their S-STEM programs, (iii) to provide increased career opportunities and job placement rates through mandatory paid co-op experiences, and (iv) to serve as a model for other universities to provide vertical transfer students access to the baccalaureate degree. The Transfer Pipeline (TiPi) project awarded 25 new scholarships per year from 2012 to 2014 to a total of 75 engineering and engineering technology transfer students. By the end of Fall 2017, 66 (88%) scholars have graduated, 5 (7%) are in process of completing their degrees, and only 4 (5%) left our university, for a 95% retention rate. The paper describes our successes and challenges. The Vertical Transfer Access to the Baccalaureate (VTAB) project recruited its first group of 25 students in Fall 2017 with the goal of recruiting a total of 78 vertical transfers over the next three years. An additional goal of the VTAB project is to conduct research and generate knowledge about the VTAB project elements that will be essential for the success of vertical transfer programs at other universities. The paper describes the research instruments, and the results from an online survey and a focus group interview of the first cohort of VTAB scholars

Phase One Research Results from a Project on Vertical Transfer Students in Engineering and Engineering Technology

This paper reports on the first phase of research on a scholarship program VTAB (Vertical Transfers’ Access to the Baccalaureate) funded by a five-year grant from the National Science Foundation (NSF) that focuses on students who transfer at the 3rd year level from 2-year schools to the engineering and engineering technology BS programs at our university. The goals of the program are: (i) to expand and diversify the engineering/technology workforce of the future, (ii) to develop linkages and articulations with 2-year schools and their S-STEM programs, (iii) to recruit, retain, and graduate 78 low-income students, and place them in industry or graduate schools, (iv) to generate knowledge about the program elements that can help other universities, and (v) to serve as a model for other universities to provide vertical transfer students access to the baccalaureate degree

Structuring Collaboration Scripts: Optimizing online group work on classroom dilemmas in teacher education

The optimal structure in collaboration scripts for serious games has appeared to be a key success factor. In this study we compare a ‘high- structured’ and ‘low-structured’ version of a mastership game where teachers-in-training discuss solutions on classroom dilemmas. We collected data on the differences in learning effects and student appreciation. The most interesting result shows that reports delivered by students that played the low-structured version received significantly higher teacher grades when compared to the high-structured version

Variable Expression of Cre Recombinase Transgenes Precludes Reliable Prediction of Tissue-Specific Gene Disruption by Tail-Biopsy Genotyping

The Cre/loxP-system has become the system of choice for the generation of conditional so-called knockout mouse strains, i.e. the tissue-specific disruption of expression of a certain target gene. We here report the loss of expression of Cre recombinase in a transgenic mouse strain with increasing number of generations. This eventually led to the complete abrogation of gene expression of the inserted Cre cDNA while still being detectable at the genomic level. Conversely, loss of Cre expression caused an incomplete or even complete lack of disruption for the protein under investigation. As Cre expression in the tissue of interest in most cases cannot be addressed in vivo during the course of a study, our findings implicate the possibility that individual tail-biopsy genotypes may not necessarily indicate the presence or absence of gene disruption. This indicates that sustained post hoc analyses in regards to efficacy of disruption for every single study group member may be required

A Genome-Wide Association Study Reveals Loci Influencing Height and Other Conformation Traits in Horses

The molecular analysis of genes influencing human height has been notoriously difficult. Genome-wide association studies (GWAS) for height in humans based on tens of thousands to hundreds of thousands of samples so far revealed ∼200 loci for human height explaining only 20% of the heritability. In domestic animals isolated populations with a greatly reduced genetic heterogeneity facilitate a more efficient analysis of complex traits. We performed a genome-wide association study on 1,077 Franches-Montagnes (FM) horses using ∼40,000 SNPs. Our study revealed two QTL for height at withers on chromosomes 3 and 9. The association signal on chromosome 3 is close to the LCORL/NCAPG genes. The association signal on chromosome 9 is close to the ZFAT gene. Both loci have already been shown to influence height in humans. Interestingly, there are very large intergenic regions at the association signals. The two detected QTL together explain ∼18.2% of the heritable variation of height in horses. However, another large fraction of the variance for height in horses results from ECA 1 (11.0%), although the association analysis did not reveal significantly associated SNPs on this chromosome. The QTL region on ECA 3 associated with height at withers was also significantly associated with wither height, conformation of legs, ventral border of mandible, correctness of gaits, and expression of the head. The region on ECA 9 associated with height at withers was also associated with wither height, length of croup and length of back. In addition to these two QTL regions on ECA 3 and ECA 9 we detected another QTL on ECA 6 for correctness of gaits. Our study highlights the value of domestic animal populations for the genetic analysis of complex traits

Precision medicine in cats:novel niemann-pick type C1 diagnosed by whole-genome sequencing

State-of-the-art health care includes genome sequencing of the patient to identify genetic variants that contribute to either the cause of their malady or variants that can be targeted to improve treatment. The goal was to introduce state-of-the-art health care to cats using genomics and a precision medicine approach. To test the feasibility of a precision medicine approach in domestic cats, a single cat that presented to the University of Missouri, Veterinary Health Center with an undiagnosed neurologic disease was whole-genome sequenced. The DNA variants from the cat were compared to the DNA variant database produced by the 99 Lives Cat Genome Sequencing Consortium. Approximately 25× genomic coverage was produced for the cat. A predicted p.H441P missense mutation was identified in NPC1, the gene causing Niemann-Pick type C1 on cat chromosome D3.47456793 caused by an adenine-to-cytosine transversion, c.1322A>C. The cat was homozygous for the variant. The variant was not identified in any other 73 domestic and 9 wild felids in the sequence database or 190 additionally genotyped cats of various breeds. The successful effort suggested precision medicine is feasible for cats and other undiagnosed cats may benefit from a genomic analysis approach. The 99 Lives DNA variant database was sufficient but would benefit from additional cat sequences. Other cats with the mutation may be identified and could be introduced as a new biomedical model for NPC1. A genetic test could eliminate the disease variant from the population

SMF-1, SMF-2 and SMF-3 DMT1 Orthologues Regulate and Are Regulated Differentially by Manganese Levels in C. elegans

Manganese (Mn) is an essential metal that can exert toxic effects at high concentrations, eventually leading to Parkinsonism. A major transporter of Mn in mammals is the divalent-metal transporter (DMT1). We characterize here DMT1-like proteins in the nematode C. elegans, which regulate and are regulated by Mn and iron (Fe) content. We identified three new DMT1-like genes in C. elegans: smf-1, smf-2 and smf-3. All three can functionally substitute for loss of their yeast orthologues in S. cerevisiae. In the worm, deletion of smf-1 or smf-3 led to an increased Mn tolerance, while loss of smf-2 led to increased Mn sensitivity. smf mRNA levels measured by QRT-PCR were up-regulated upon low Mn and down-regulated upon high Mn exposures. Translational GFP-fusions revealed that SMF-1 and SMF-3 strongly localize to partially overlapping apical regions of the gut epithelium, suggesting a differential role for SMF-1 and SMF-3 in Mn nutritional intake. Conversely, SMF-2 was detected in the marginal pharyngeal epithelium, possibly involved in metal-sensing. Analysis of metal content upon Mn exposure in smf mutants revealed that SMF-3 is required for normal Mn uptake, while smf-1 was dispensable. Higher smf-2 mRNA levels correlated with higher Fe content, supporting a role for SMF-2 in Fe uptake. In smf-1 and smf-3 but not in smf-2 mutants, increased Mn exposure led to decreased Fe levels, suggesting that both metals compete for transport by SMF-2. Finally, SMF-3 was post-translationally and reversibly down-regulated following Mn-exposure. In sum, we unraveled a complex interplay of transcriptional and post-translational regulations of 3 DMT1-like transporters in two adjacent tissues, which regulate metal-content in C. elegans