Study of C- and O-glycosylflavones in sugarcane extracts using liquid chromatography: exact mass measurement mass spectrometry

The flavonoids present in sugarcane (Saccharum officinarum) extracts were analyzed by liquid chromatography - mass spectrometry (LC-MS), and a study of the fragmentation patterns of selected flavonoids was conducted using orthogonal acceleration time-of-flight electrospray ionization mass spectrometry (ESI-oa-ToF MS). Seven C- and O-glycosylflavones were identified in the extracts, namely, schaftoside, isoschaftoside, luteolin-8-C-(rhamnosylglucoside), vitexin, orientin, tricin-7-O-neohesperidoside and tricin-7-O-glucoside. Of these, five were identified in the absence of direct comparison with their respective standards. The described method also permitted the differentiation of the 6-C and 8-C isomeric flavones, schaftoside and isoschaftoside. The combination of fragmentation data and exact mass measurement showed to be complimentary to the HPLC-UV-MS techniques previously utilized for isomers discrimination in sugarcane studies.Os flavonóides presentes nos extratos de cana-de-açúcar (Saccharum officinarum) foram analisados por cromatografia líquida acoplada à espectrometria de massas (LC-MS), e o estudo da rota de fragmentação dos flavonóides selecionados foi realizado utilizando a espectrometria de massas com aceleração ortogonal por tempo de vôo e ionização eletrospray (ESI-oa-ToF MS). Sete flavonas C- e O-glicosiladas foram identificadas nos extratos: schaftosídeo, isoschaftosídeo, luteolina-8-C-(ramnosilglicosídeo), vitexina, orientina, tricina-7-O-neohesperidosídeo e tricina-7-O-glicosídeo. Destas, cinco foram identificadas sem comparação direta com seus respectivos padrões. O método descrito também permitiu a diferenciação das 6-C e 8-C flavonas isoméricas, schaftosídeo e isoschaftosídeo. A combinação dos dados de fragmentação e a medida de massa exata mostraram ser complementares às técnicas de HPLC-UV-MS previamente utilizadas para discriminação de isômeros nos estudos da cana-de-açúcar.FAPESPCNP

Very superstitious? A preliminary investigation of pigeons’ body position during a matching-to-sample task under differential and common outcome conditions.

The delayed matching-to-sample (DMS) task is widely employed to assess memory in a range of non-human animals. On the standard “common outcomes” (CO) DMS task, correct performance following either sample stimulus results in reinforcement. In contrast, on a “differential outcomes” (DO) DMS task, the outcome following either sample stimulus is different. One of the most consistent findings in the comparative literature is that performance under a DO condition is superior to that under a CO condition. The superior performance is attributed to the fact the DO condition enhances memory for the sample stimulus by tagging each sample with a discrete reward. Here, we investigate an alternative possibility, that pigeons use positional mediation during the delay under DO, but not CO, conditions. To test this, we tracked the head position of pigeons performing a DO (n = 4) or CO (n = 4) task. Consistent with the positional mediation account, all subjects in the DO condition displayed evidence of positional mediation. Surprisingly, positional mediation was not unique to subjects in the DO condition, with subjects in the CO condition also displaying evidence of mediation.&nbsp

Matriptase regulates c-Met mediated proliferation and invasion in inflammatory breast cancer.

The poor prognosis for patients with inflammatory breast cancer (IBC) compared to patients with other types of breast cancers emphasizes the need to better understand the molecular underpinnings of this disease with the goal of developing effective targeted therapeutics. Dysregulation of matriptase expression, an epithelial-specific member of the type II transmembrane serine protease family, has been demonstrated in many different cancer types. To date, no studies have assessed the expression and potential pro-oncogenic role of matriptase in IBC. We examined the functional relationship between matriptase and the HGF/c-MET signaling pathway in the IBC cell lines SUM149 and SUM190, and in IBC patient samples. Matriptase and c-Met proteins are localized on the surface membrane of IBC cells and their expression is strongly correlated in infiltrating cancer cells and in the cancer cells of lymphatic emboli in patient samples. Abrogation of matriptase expression by silencing with RNAi or inhibition of matriptase proteolytic activity with a synthetic inhibitor impairs the conversion of inactive pro-HGF to active HGF and subsequent c-Met-mediated signaling, leading to efficient impairment of proliferation and invasion of IBC cells. These data show the potential of matriptase inhibitors as a novel targeted therapy for IBC, and lay the groundwork for the development and testing of such drugs

Control of the Continuity Equation with a Non Local Flow

This paper focuses on the optimal control of weak (i.e. in general non smooth) solutions to the continuity equation with non local flow. Our driving examples are a supply chain model and an equation for the description of pedestrian flows. To this aim, we prove the well posedness of a class of equations comprising these models. In particular, we prove the differentiability of solutions with respect to the initial datum and characterize its derivative. A necessary condition for the optimality of suitable integral functionals then follows

Evolution of cooperation in device-to-device communication

Device-to-device (D2D) communications are a promising paradigm to improve spectral efficiency in cellular wireless networks by enabling peer to peer communication. In particular, short D2D links can be used to relay data to reduce the burden on core infrastructure. However, this relies on some mechanism to either enforce or incentivise nodes to donate their resources in order to act as a relay without any guarantee that this will be reciprocated in the future. Indirect reciprocity has been well studied from the perspective of human behaviour, proposing mechanisms and conditions under which such behaviour naturally evolves. In this paper we consider D2D networks that formulate the decision to share resources as a donation game using a model of social comparison and examine the conditions under which cooperation evolves without the need for a central authority. Experimentation shows that the emergence of cooperation is sensitive to network conditions, such as node density and noise

Selective nitrogen adsorption via backbonding in a metal-organic framework with exposed vanadium sites.

Industrial processes prominently feature π-acidic gases, and an adsorbent capable of selectively interacting with these molecules could enable important chemical separations1-4. Biological systems use accessible, reducing metal centres to bind and activate weakly π-acidic species, such as N2, through backbonding interactions5-7, and incorporating analogous moieties into a porous material should give rise to a similar adsorption mechanism for these gaseous substrates8. Here, we report a metal-organic framework featuring exposed vanadium(II) centres capable of back-donating electron density to weak π acids to successfully target π acidity for separation applications. This adsorption mechanism, together with a high concentration of available adsorption sites, results in record N2 capacities and selectivities for the removal of N2 from mixtures with CH4, while further enabling olefin/paraffin separations at elevated temperatures. Ultimately, incorporating such π-basic metal centres into porous materials offers a handle for capturing and activating key molecular species within next-generation adsorbents

Presenting evidence-based health information for people with multiple sclerosis : the IN-DEEP project protocol

Background - Increasingly, evidence-based health information, in particular evidence from systematic reviews, is being made available to lay audiences, in addition to health professionals. Research efforts have focused on different formats for the lay presentation of health information. However, there is a paucity of data on how patients integrate evidence-based health information with other factors such as their preferences for information and experiences with information-seeking. The aim of this project is to explore how people with multiple sclerosis (MS) integrate health information with their needs, experiences, preferences and values and how these factors can be incorporated into an online resource of evidence-based health information provision for people with MS and their families.Methods - This project is an Australian-Italian collaboration between researchers, MS societies and people with MS. Using a four-stage mixed methods design, a model will be developed for presenting evidence-based health information on the Internet for people with MS and their families. This evidence-based health information will draw upon systematic reviews of MS interventions from The Cochrane Library. Each stage of the project will build on the last. After conducting focus groups with people with MS and their family members (Stage 1), we will develop a model for summarising and presenting Cochrane MS reviews that is integrated with supporting information to aid understanding and decision making. This will be reviewed and finalised with people with MS, family members, health professionals and MS Society staff (Stage 2), before being uploaded to the Internet and evaluated (Stages 3 and 4).Discussion - This project aims to produce accessible and meaningful evidence-based health information about MS for use in the varied decision making and management situations people encounter in everyday life. It is expected that the findings will be relevant to broader efforts to provide evidence-based health information for patients and the general public. The international collaboration also permits exploration of cultural differences that could inform international practice.<br /