3,412 research outputs found
Methanogenesis by Methanosarcina acetivorans involves two structurally and functionally distinct classes of heterodisulfide reductase
Biochemical studies have revealed two distinct classes of Coenzyme B-Coenzyme M heterodisulfide (CoB-S-S-CoM) reductase (Hdr), a key enzyme required for anaerobic respiration in methaneproducing archaea. A cytoplasmic HdrABC enzyme complex is found in most methanogens, whereas a membrane-bound HdrED complex is found exclusively in members of the order Methanosarcinales. Unexpectedly, genomic data indicate that multiple copies of both Hdr classes are found in all sequenced Methanosarcinales genomes. The Methanosarcina acetivorans hdrED1 operon is constitutively expressed and required for viability under all growth conditions examined, consistent with HdrED being the primary Hdr. HdrABC appears to be specifically involved in methylotrophic methanogenesis, based on reduced growth and methanogenesis rates of an hdrA1C1B1 mutant on methylotrophic substrates and downregulation of the genes during growth on acetate. This conclusion is further supported by phylogenetic analysis showing that the presence of hdrA1 in an organism is specifically correlated with the presence of genes for methylotrophic methanogenesis. Examination of mRNA abundance in methanol-grown DhdrA1C1B1 strains relative to wild-type revealed upregulation of genes required for synthesis of (di)methylsulfide and for transport and biosynthesis of CoB-SH and CoM-SH, suggesting that the mutant has a defect in electron transfer from ferredoxin to CoB-S-S-CoM that causes cofactor limitation
An Optimal Design for Universal Multiport Interferometers
Universal multiport interferometers, which can be programmed to implement any
linear transformation between multiple channels, are emerging as a powerful
tool for both classical and quantum photonics. These interferometers are
typically composed of a regular mesh of beam splitters and phase shifters,
allowing for straightforward fabrication using integrated photonic
architectures and ready scalability. The current, standard design for universal
multiport interferometers is based on work by Reck et al (Phys. Rev. Lett. 73,
58, 1994). We demonstrate a new design for universal multiport interferometers
based on an alternative arrangement of beam splitters and phase shifters, which
outperforms that by Reck et al. Our design occupies half the physical footprint
of the Reck design and is significantly more robust to optical losses.Comment: 8 pages, 4 figure
Laser Phase and Frequency Stabilization Using Atomic Coherence
We present a novel and simple method of stabilizing the laser phase and
frequency by polarization spectroscopy of an atomic vapor. In analogy to the
Pound-Drever-Hall method, which uses a cavity as a memory of the laser phase,
this method uses atomic coherence (dipole oscillations) as a phase memory of
the transmitting laser field. A preliminary experiment using a distributed
feedback laser diode and a rubidium vapor cell demonstrates a
shot-noise-limited laser linewidth reduction (from 2 MHz to 20 kHz). This
method would improve the performance of gas-cell-based optical atomic clocks
and magnetometers and facilitate laser-cooling experiments using narrow
transitions.Comment: 7 pages, 6 figures, appendix on the derivation of Eq.(3) (transfer
function for a polarization-spectroscopy-based frequency discriminator) has
been adde
Zener double exchange from local valence fluctuations in magnetite
Magnetite (FeO) is a mixed valent system where electronic
conductivity occurs on the B-site (octahedral) iron sublattice of the spinel
structure. Below K, a metal-insulator transition occurs which is
argued to arise from the charge ordering of 2+ and 3+ iron valences on the
B-sites (Verwey transition). Inelastic neutron scattering measurements show
that optical spin waves propagating on the B-site sublattice (80 meV) are
shifted upwards in energy above due to the occurrence of B-B
ferromagnetic double exchange in the mixed valent metallic phase. The double
exchange interaction affects only spin waves of symmetry, not all
modes, indicating that valence fluctuations are slow and the double exchange is
constrained by electron correlations above .Comment: 4 pages, 5 figure
Quantum rainbow scattering at tunable velocities
Elastic scattering cross sections are measured for lithium atoms colliding
with rare gas atoms and SF6 molecules at tunable relative velocities down to
~50 m/s. Our scattering apparatus combines a velocity-tunable molecular beam
with a magneto-optic trap that provides an ultracold cloud of lithium atoms as
a scattering target. Comparison with theory reveals the quantum nature of the
collision dynamics in the studied regime, including both rainbows as well as
orbiting resonances
Systematic errors in strong gravitational lensing reconstructions, a numerical simulation perspective
We present the analysis of a sample of twenty-four SLACS-like galaxy-galaxy
strong gravitational lens systems with a background source and deflectors from
the Illustris-1 simulation. We study the degeneracy between the complex mass
distribution of the lenses, substructures, the surface brightness distribution
of the sources, and the time delays. Using a novel inference framework based on
Approximate Bayesian Computation, we find that for all the considered lens
systems, an elliptical and cored power-law mass density distribution provides a
good fit to the data. However, the presence of cores in the simulated lenses
affects most reconstructions in the form of a Source Position Transformation.
The latter leads to a systematic underestimation of the source sizes by 50 per
cent on average, and a fractional error in of around
per cent. The analysis of a control sample of twenty-four lens systems, for
which we have perfect knowledge about the shape of the lensing potential, leads
to a fractional error on of per cent. We find no
degeneracy between complexity in the lensing potential and the inferred amount
of substructures. We recover an average total projected mass fraction in
substructures of at the 68 per cent
confidence level in agreement with zero and the fact that all substructures had
been removed from the simulation. Our work highlights the need for
higher-resolution simulations to quantify the lensing effect of more realistic
galactic potentials better, and that additional observational constraint may be
required to break existing degeneracies.Comment: Accepted by MNRA
Non-degenerate four-wave mixing in rubidium vapor: transient regime
We investigate the transient response of the generated light from Four-Wave
Mixing (FWM) in the diamond configuration using a step-down field excitation.
The transients show fast decay times and oscillations that depend on the
detunings and intensities of the fields. A simplified model taking into account
the thermal motion of the atoms, propagation, absorption and dispersion effects
shows qualitative agreement with the experimental observations with the energy
levels in rubidium (5S1/2, 5P1/2, 5P3/2 and 6S1/2). The atomic polarization
comes from all the contributions of different velocity classes of atoms in the
ensemble modifying dramatically the total transient behavior of the light from
FWM.Comment: 11 pages, 11 figures, to be published in Physical Review
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