Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli

peer-reviewedThe bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin transporter protein. Cloning of these operons in E. coli TunerTM (DE3) on a pETcoco-2 derived vector resulted in successful secretion of both bacteriocins. A corresponding approach, involving the construction of vectors containing different combinations of these genes, revealed that the structural and the transporter genes alone are sufficient to permit heterologous production and secretion in this host. Even though the accessory protein, usually associated with optimal disulfide bond formation, was not required for bacteriocin synthesis, its presence did result in greater pediocin PA-1 production. The simplicity of the system and the fact that the associated bacteriocins could be recovered from the extracellular medium provides an opportunity to facilitate protein engineering and the overproduction of biologically-active bacteriocins at industrial scale. Additionally, this system could enable the characterization of new bacteriocin operons where genetic tools are not available for the native producers

A vigorous activity cycle mimicking a planetary system in HD200466

Stellar activity can be a source of radial velocity (RV) noise and can reproduce periodic RV variations similar to those produced by an exoplanet. We present the vigorous activity cycle in the primary of the visual binary HD200466, a system made of two almost identical solar-type stars with an apparent separation of 4.6 arcsec at a distance of 44+/-2 pc. High precision RV over more than a decade, adaptive optics (AO) images, and abundances have been obtained for both components. A linear trend in the RV is found for the secondary. We assumed that it is due to the binary orbit and once coupled with the astrometric data, it strongly constrains the orbital solution of the binary at high eccentricities (e~0.85) and quite small periastron of ~21 AU. If this orbital motion is subtracted from the primary radial velocity curve, a highly significant (false alarm probability <0.1%) period of about 1300 d is obtained, suggesting in a first analysis the presence of a giant planet, but it turned out to be due to the stellar activity cycle. Since our spectra do not include the Ca~II resonance lines, we measured a chromospheric activity indicator based on the Halpha line to study the correlation between activity cycles and long-term activity variations. While the bisector analysis of the line profile does not show a clear indication of activity, the correlation between the Halpha line indicator and the RV measurements identify the presence of a strong activity cycle.Comment: Accepted on Astronomy and Astrophysics Main Journal 2014, 16 pages, 18 figure

A possibly inflated planet around the bright, young star DS Tuc A

The origin of the observed diversity of planetary system architectures is one of the main topic of the exoplanetary research. The detection of a statistically significant sample of planets around young stars allows us to study the early stages of planet formation and evolution, but only a handful of them is known so far. In this regard, a considerable contribution is expected from the NASA TESS satellite, which is now performing a survey of $\sim 85 \%$ of the sky to search for short-period transiting planets In its first month of operations, TESS found a planet candidate with an orbital period of 8.14 days around a member of the Tuc-Hor young association ($\sim$ 40 Myr), the G6V main component of the binary system DS\,Tuc. If confirmed, it would be the first transiting planet around a young star suitable for radial velocity and/or atmospheric characterization. We aim to validate the planetary nature of this companion and to measure its orbital and physical parameters. We obtain accurate planet parameters by coupling an independent reprocessing of the TESS light curve with improved stellar parameters and the dilution caused by the binary companion; we analyse high precision archival radial velocities to impose an upper limit of about 0.1 M$_{\rm Jup}$ on the planet mass; we finally rule out the presence of external companions beyond 40 au with adaptive optics images. We confirm the presence of a young, giant (${\rm R} = 0.50$ R$_{\rm Jup}$) planet having a not negligible possibility to be inflated (theoretical mass $\lesssim 20$ M$_{\oplus}$) around DS\,Tuc~A. We discuss the feasibility of mass determination, Rossiter-McLaughlin analysis and atmosphere characterization, allowed by the brightness of the star.Comment: 12 pages, 11 figures. Accepted for publication in A&

The SPHERE data center: a reference for high contrast imaging processing

The objective of the SPHERE Data Center is to optimize the scientific return of SPHERE at the VLT, by providing optimized reduction procedures, services to users and publicly available reduced data. This paper describes our motivation, the implementation of the service (partners, infrastructure and developments), services, description of the on-line data, and future developments. The SPHERE Data Center is operational and has already provided reduced data with a good reactivity to many observers. The first public reduced data have been made available in 2017. The SPHERE Data Center is gathering a strong expertise on SPHERE data and is in a very good position to propose new reduced data in the future, as well as improved reduction procedures.Comment: SF2A proceeding

Operacionalização de uma planta de pirólise rápida de biomassa com reator de leito fluidizado.

Neste trabalho são discutidos os principais problemas encontrados durante a operação da planta piloto de pirólise rápida de biomassa, pertencente à Universidade Estadual de Campinas- Unicamp, Brasil. Essa planta foi ajustada para a produção de bio-óleo, a partir de resíduos de biomassa de pequeno tamanho. Durante os testes, os principais problemas identificados foram: escoamento de vapores da pirólise pelo sistema de alimentação de biomassa; travamento da rosca de alimentação de biomassa; segregação da biomassa no leito; sinterização do material inerte; acúmulo de biomassa e de carvão vegetal dentro do reator; dificuldade para o aquecimento do inerte e baixa eficiência de recuperação de bio-óleo. Os resultados dos testes exploratórios permitiram definir condições operacionais para possibilitar o trabalho estável do reator, aumentar o conhecimento sobre os fenômenos que ocorrem durante a pirólise rápida em leito fluidizado, além do estabelecimento de faixas operacionais para vários fatores independentes, visando a futuros trabalhos de otimização experimental

The origin of R CrA variability: A complex triple system hosting a disk

R~CrA is the brightest member of the Coronet star forming region and it is the closest Herbig AeBe star with a spectrum dominated by emission lines. Its luminosity has been monitored since the end of the 19th century, but the origin of its variability, which shows a stable period of $65.767\pm 0.007$~days, is still unknown. We studied photometric and spectroscopic data for this star to investigate the nature of the variability of R~CrA. We exploited the fact that near infrared luminosity of the Herbig AeBe stars is roughly proportional to the total luminosity of the stars to derive the absorption, and then mass and age of R~CrA. In addition, we model the periodic modulation of the light curve as due to partial attenuation of a central binary by a circumbinary disk. This model reproduces very well the observations. We found that the central object in R~CrA is a very young ($1.5\pm 1.5$~Myr), highly absorbed ($A_V=5.47\pm 0.4$~mag) binary; we obtain masses of $M_A=3.02\pm 0.43$~M$_\odot$ and $M_B=2.32\pm 0.35$~M$_\odot$ for the two components. We propose that the secular decrease of the R~CrA apparent luminosity is due to a progressive increase of the disk absorption. This might be related to precession of a slightly inclined disk caused by the recently discovered M-dwarf companion. Thus, R~CrA may be a triple system hosting a disk.Comment: Accepted for publication in A&A. 14 pages, 11 figure

Characterizing HR3549B using SPHERE

Aims. In this work, we characterize the low mass companion of the A0 field star HR3549. Methods. We observed HR3549AB in imaging mode with the the NIR branch (IFS and IRDIS) of SPHERE@VLT, with IFS in YJ mode and IRDIS in the H band. We also acquired a medium resolution spectrum with the IRDIS long slit spectroscopy mode. The data were reduced using the dedicated SPHERE GTO pipeline, purposely designed for this instrument. We employed algorithms such as PCA and TLOCI to reduce the speckle noise. Results. The companion was clearly visible both with IRDIS and IFS.We obtained photometry in four different bands as well as the astrometric position for the companion. Based on our astrometry, we confirm that it is a bound object and put constraints on its orbit. Although several uncertainties are still present, we estimate an age of ~100-150 Myr for this system, yielding a most probable mass for the companion of 40-50MJup and T_eff ~300-2400 K. Comparing with template spectra points to a spectral type between M9 and L0 for the companion, commensurate with its position on the color-magnitude diagram.Comment: Accepted by A&A, 13 pages, 10 Figures (Figures 9 and 10 degraded to reduce the dimension

AF Lep b: the lowest mass planet detected coupling astrometric and direct imaging data

Aims. Using the direct imaging technique we searched for low mass companions around the star AF Lep that presents a significant proper motion anomaly (PMa) signal obtained from the comparison of Hipparcos and Gaia eDR3 catalogs. Methods. We observed AF Lep in two epochs with VLT/SPHERE using its subsystems IFS and IRDIS in the near-infrared (NIR) covering wavelengths ranging from the Y to the K spectral bands (between 0.95 and 2.3 {\mu}m). The data were then reduced using the high-contrast imaging techniques angular differential imaging (ADI) and spectral differential imaging (SDI) to be able to retrieve the signal from low mass companions of the star. Results. A faint companion was retrieved at a separation of ~0.335" from the star and with a position angle of ~70.5 deg in the first epoch and with a similar position in the second epoch. This corresponds to a projected separation of ~9 au. The extracted photometry allowed us to estimate for the companion a mass between 2 and 5 MJup. This mass is in good agreement with what is expected for the dynamic mass of the companion deduced using astrometric measures (5.2-5.5 MJup). This is the first companion with a mass well below the deuterium burning limit discovered coupling direct imaging with PMa measures. Orbit fitting done using the orvara tool allowed to further confirm the companion mass and to define its main orbital parameters.Comment: 10 pages, 8 Figures, accepted for publication on A&