Global Journalist: Siege on Moscow amid global terrorism crisis

Global Journalist, October 31, 2002 show: On October 31, 2002, Chechen terrorist rebels sieged a theatre in Moscow taking hundreds of occupants hostage. Byron Scott discusses the media coverage of the Moscow theatre hostage crisis with Russian journalists and UN media correspondents. Amid the global rise of terrorism, Global Journalist raises concerns for media ethics and press freedom as tension increases between Chechnya and the Russian Federation. Host: Byron Scott. Guests: Akaki Gogichaishvili, Betsy Pisik, Alexander Merkushev, Andrei Zolotov. Producers: Sarah Fajardo. Directors: Pat Akers

Global Journalist: America's hunt for terrorists and Venezuela’s political crisis

On this December 19, 2002 program, journalists discuss a new U.S. order permitting the assassination of terrorists and a brewing political crisis Venezuela and what it means for global oil markets

Global Journalist: GJ talks 2003 G8 Summit (2003)

This June 5, 2003 Global Journalist program features guest host Byron Scott speaking with four international journalists about their perspectives on the G8 summit held in Évian, France. Host: Byron Scott. Guests: Shaowen Lin (@LinShaowen1), Alexander Merkushev, Alberto Romagnoli, Edwin Naidu. Producers: Sarah Cayton, Yusef Catiango. Sound Engineer: Pat Akers

Amine-Reactive BODIPY Dye: Spectral Properties and Application for Protein Labeling

A boron-dipyrromethene (BODIPY) derivative reactive towards amino groups of proteins (NHS-Ph-BODIPY) was synthesized. Spectroscopic and photophysical properties of amine-reactive NHS-Ph-BODIPY and its non-reactive precursor (COOH-Ph-BODIPY) in a number of organic solvents were investigated. Both fluorescent dyes were characterized by green absorption (521–532 nm) and fluorescence (538–552 nm) and medium molar absorption coefficients (46,500–118,500 M−1·cm−1) and fluorescence quantum yields (0.32 – 0.73). Solvent polarizability and dipolarity were found to play a crucial role in solvent effects on COOH-Ph-BODIPY and NHS-Ph-BODIPY absorption and emission bands maxima. Quantum-chemical calculations were used to show why solvent polarizability and dipolarity are important as well as to understand how the nature of the substituent affects spectroscopic properties of the fluorescent dyes. NHS-Ph-BODIPY was used for fluorescent labeling of a number of proteins. Conjugation of NHS-Ph-BODIPY with bovine serum albumin (BSA) resulted in bathochromic shifts of absorption and emission bands and noticeable fluorescence quenching (about 1.5 times). It was demonstrated that the sensitivity of BSA detection with NHS-Ph-BODIPY was up to eight times higher than with Coomassie brilliant blue while the sensitivity of PII-like protein PotN (PotN) detection with NHS-Ph-BODIPY and Coomassie brilliant blue was almost the same. On the basis of the molecular docking results, the most probable binding sites of NHS-Ph-BODIPY in BSA and PotN and the corresponding binding free energies were estimated