449 research outputs found
Phytohormonal effects on rhizosphere processes of maize (Zea mays L.) under phosphorus deficiency
Effects of the hormones indole-3-acetic acid (IAA), gibberellic acid (GA3), and trans-zeatin (t-Z) on growth, P status and rhizosphere processes of maize (Zea mays L., cv. 'Bezemara') were investigated in a pot experiment at two levels of phosphorus availability (+P: water soluble phosphate and -P: sparingly soluble tricalcium phosphate). Six weeks after seed germination, plants were harvested and analysed for dry weight, shoot length, root surface, P concentration, acid phosphatases activity (acid Pase) in shoot and rhizosphere and the content of carboxylic acids and sugars in the rhizosphere. ANOVA was used to estimate the effects of treatments on measured parameters. Hormone application via rhizosphere had a highly significant effect on the growth of whole plants, their P status and rhizosphere processes. GA3 and t-Z promoted quantitatively shoot and root growth and morphological changes, whereas IAA affected the chemical composition of the rhizosphere. In several parameters, the effects of hormone treatment depended on the P status of plants indicating different sensitivity of +P and -P plants to plant growth regulator (PGR) application (significant interaction of hormone application Ă P availability). The findings help to improve our knowledge, why PGR treatments and plant growth promoting rhizo-microorganisms have varying effects on plants depending on growth conditions
Root exudation of phloridzin by apple seedlings (Malus x domestica Borkh.) with symptoms of apple replant disease
This study investigates the occurrence of the flavonoid phloridzin (phloretin-2'-O-ÎČ-D-glucoside) in root exudates of apple seedlings showing growth reduction related to apple replant disease (ARD). The disease is most likely caused by a complex of soil-borne fungi and bacteria, but the etiology remains to be elucidated. Information on specific exudation processes in the rhizosphere of apple seedlings could contribute to our understanding of the conditions triggering ARD development.To procure ARD symptoms, apple seedlings (Malus x domestica Borkh.) were grown in ARD-conductive soil. Root exudates were collected by submerging the roots in a solution of 0.05 mM CaCl2 for a period of 4 h. The fraction of phenolic root exudates was analyzed using HPLC/DAD (high performance liquid chromatography/diode array detector).Results suggest that (i) phloridzin is a constant root exudate of apple seedlings. It was the most abundant phenol in the collected exudates from replant-diseased as well as healthy seedlings. (ii) Phloridzin exudation, related to root dry matter, was the most intensive at the onset of ARD symptom development and lower during the period when symptoms were most severe or outgrown. (iii) In comparison to healthy seedlings, the phloridzin exudation of apple replantdiseased seedlings was significantly higher only at the onset of ARD symptom development, suggesting a response of the plants to infection.The finding of phloridzin in the root exudates of Malus x domestica Borkh. might have consequences for research on the etiology of ARD. Specialized pathogenic microorganisms could be attracted by this distinct compound. Since it is very characteristic of apple plants, phloridzin might be the compound that ARD-causing microorganisms utilize to recognize their host. For practical applications, phloridzin root exudation could therefore be a parameter in evaluating ARD-susceptibility of different rootstocks
Occurrence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeis guineensis) rhizosphere in Cameroon
Phosphorus replenishment, particularly in smallholder agriculture, remains a challenge as it is mainly fertilizer dependent. While the use of soluble mineral phosphate fertilizers is the obvious best means tocombat phosphate deficiency in Cameroon, their use is limited by their high cost and availability at farmerâs level. This study was aimed at maintaining the fertility of Cameroon soils by biological means,in order to improve agricultural production, using low inputs technology. Isolates were obtained from oil palm tree (Elaeis guineensis) root fragments and rhizospheric soils and their activity in mobilizingphosphate from insoluble sources was evaluated on agar plates and liquid culture media containing sparingly soluble phosphates. At the end of incubation time, it appeared that, phosphate solubilizationresulted from a combined effect of pH decrease of the media and organic acids production. Furthermore, each of the tested isolates was able to produce at least one of the most important organic acids such as citrate, malate and tartrate. Among the ten isolates tested, three were identified as Pseudomonas fluorescens and would be considered as potential biofertilizers
Theoretical study of the hydrated Gd3+ ion: Structure, dynamics, and charge transfer
The dynamical processes taking place in the first coordination shells of the gadolinium (III) ion are important for improving the contrast agent efficiency in magnetic-resonance imaging. An extensive study of the gadolinium (III) ion solvated by a water cluster is reported, based on molecular dynamics simulations. The AMOEBA force field [P. Y. Ren and J. W. Ponder, J. Phys. Chem. B 107, 5933 (2003)] that includes many-body polarization effects is used to describe the interactions among water molecules, and is extended here to treat the interactions between them and the gadolinium ion. In this purpose accurate ab initio calculations have been performed on Gd3+-H2O for extracting the relevant parameters. Structural data of the first two coordination shells and some dynamical properties such as the water exchange rate between the first and second coordination shells are compared to available experimental results. We also investigate the charge transfer processes between the ion and its solvent, using a fluctuating charges model fitted to reproduce electronic structure calculations on [Gd(H2O)n]3+ complexes, with n ranging from 1 to 8. Charge transfer is seen to be significant (about one electron) and correlated with the instantaneous coordination of the ion
Simulating the soil phosphorus dynamics of four longâterm field experiments with a novel phosphorus model
Phosphorus is a nonrenewable resource, which is required for crop growth and to maintain high yields. The soil P cycle is very complex, and new model approaches can lead to a better understanding of those processes and further guide to research gaps. The objective of this study was to present a P-submodel, which has been integrated in the existing Carbon Candy Balance (CCB) model that already comprises a C and N module. The P-module is linked to the C mineralization and the associated C-pools via the C/P ratio of fresh organic material. Besides the organic P cycling, the module implies a plant-available P-pool (Pav), which is in a dynamic equilibrium with the nonavailable P-pool (Pna) that comprises the strongly sorbed and occluded P fraction. The model performance was tested and evaluated on four long-term field experiments with mineral P fertilization, farmyard manure as organic fertilizer and control plots without fertilization. The C dynamics and the Pav dynamics were modelled with overall good results. The relative RMSE for the C was below 10% for all treatments, while the relative RMSE for Pav was below 15% for most treatments. To accommodate for the rather small variety of available P-models, the presented CNP-model is designed for agricultural field sites with a relatively low data input, namely air temperature, precipitation, soil properties, yields and management practices. The CNP-model offers a low entry threshold model approach to predict the C-N and now the P dynamics of agricultural soils.Fachagentur Nachwachsende Rohstoffe
http://dx.doi.org/10.13039/501100010812Peer Reviewe
Ionization of Strong Electrolytes. IX. Proton Magnetic Resonance in the Ternary System HNO 3
Regional wall motion abnormalities at rest and stress in patients with end-stage renal disease diagnosed by cardiac magnetic resonance imaging
Transient versus static electron spin relaxation in Mn2+ complexes relevant as MRI contrast agents
[Abstract] The zero-field splitting (ZFS) parameters of the [Mn(EDTA)(H2O)]2â·2H2O and [Mn(MeNO2A)(H2O)]·2H2O systems were estimated by using DFT and ab initio CASSCF/NEVPT2 calculations (EDTA = 2,2âČ,2âł,2âŽ-(ethane-1,2-diylbis(azanetriyl))tetraacetate; MeNO2A = 2,2âČ-(7-methyl-1,4,7-triazonane-1,4-diyl)diacetate). Subsequent molecular dynamics calculations performed within the atom-centered density matrix propagation (ADMP) approach provided access to the transient and static ZFS parameters, as well as to the correlation time of the transient ZFS. The calculated ZFS parameters present a reasonable agreement with the experimental values obtained from the analysis of 1H relaxation data. The correlation times calculated for the two systems investigated turned out to be very short (Ïc ⌠0.02â0.05 ps), which shows that the transient ZFS is modulated by molecular vibrations. On the contrary, the static ZFS is modulated by the rotation of the complexes in solution, which for the small complexes investigated here is characterized by rotational correlation times of ÏR ⌠35â60 ps. As a result, electron spin relaxation in small Mn2+complexes is dominated by the static ZFS.España. Ministerio de EconomĂa y Competitividad; CTQ2013-43243-PEspaña. Ministerio de EconomĂa y Competitividad; CTQ2015-71211-RED
Differences in labile soil organic matter explain potential denitrification and denitrifying communities in a long-term fertilization experiment
Content and quality of organic matter (OM) may strongly affect the denitrification potential of soils. In particular, the impact of soil OM fractions of differing bioavailability (soluble, particulate, and mineral-associated OM) on denitrification remains unresolved. We determined the potential N2O and N2 as well as CO2 production for samples of a Haplic Chernozem from six treatment plots (control, mineral N and NP, farmyard manure - FYM, and FYM + mineral N or NP) of the Static Fertilization Experiment Bad LauchstĂ€dt (Germany) as related to OM properties and denitrifier gene abundances. Soil OM was analyzed for bulk chemical composition (13C-CPMAS NMR spectroscopy) as well as water-extractable, particulate, and mineral-associated fractions. Soils receiving FYM had more total OM and larger portions of labile fractions such as particulate and water-extractable OM. Incubations were run under anoxic conditions without nitrate limitation for seven days at 25 °C in the dark to determine the denitrification potential (N2O and N2) using the acetylene inhibition technique. Abundances of nirS, nirK, and nosZ (I + II) genes were analyzed before and after incubation. The denitrification potential, defined as the combined amount of N released as N2O + N2 over the experimental period, was larger for plots receiving FYM (25.9â27.2 mg N kgâ1) than pure mineral fertilization (17.1â19.2 mg N kgâ1) or no fertilization (12.6 mg N kgâ1). The CO2 and N2O production were well related and up to three-fold larger for FYM-receiving soils than under pure mineral fertilization. The N2 production differed significantly only between all manured and non-manured soils. Nitrogenous gas emissions related most closely to water-extractable organic carbon (WEOC), which again related well to free particulate OM. The larger contribution of N2 production in soils without FYM application, and thus, with less readily decomposable OM, coincided with decreasing abundances of nirS genes (NO2â reductase) and increasing abundances of genes indicating complete denitrifying organisms (nosZ I) during anoxic conditions. Limited OM sources, thus, favored a microbial community more efficient in resource use. This study suggests that WEOC, representing readily bioavailable OM, is a straightforward indicator of the denitrification potential of soils
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