The Lyman Continuum Escape Survey: Ionizing Radiation from [O III]-Strong Sources at a Redshift of 3.1

We present results from the LymAn Continuum Escape Survey (LACES), a Hubble Space Telescope (HST) program designed to characterize the ionizing radiation emerging from a sample of Lyman alpha emitting galaxies at redshift $z\simeq 3.1$. As many show intense [O III] emission characteristic of $z>6.5$ star-forming galaxies, they may represent valuable low redshift analogs of galaxies in the reionization era. Using HST Wide Field Camera 3 / UVIS $F336W$ to image Lyman continuum emission, we investigate the escape fraction of ionizing photons in this sample. For 61 sources, of which 77% are spectroscopically confirmed and 53 have measures of [O III] emission, we detect Lyman continuum leakage in 20%, a rate significantly higher than is seen in individual continuum-selected Lyman break galaxies. We estimate there is a 98% probability that $\leq 2$ of our detections could be affected by foreground contamination. Fitting multi-band spectral energy distributions (SEDs) to take account of the varying stellar populations, dust extinctions and metallicities, we derive individual Lyman continuum escape fractions corrected for foreground intergalactic absorption. We find escape fractions of 15 to 60% for individual objects, and infer an average 20% escape fraction by fitting composite SEDs for our detected samples. Surprisingly however, even a deep stack of those sources with no individual $F336W$ detections provides a stringent upper limit on the average escape fraction of less than 0.5%. We examine various correlations with source properties and discuss the implications in the context of the popular picture that cosmic reionization is driven by such compact, low metallicity star-forming galaxies.Comment: 26 pages, 16 figures, accepted for publication in Ap

Spectroscopic Constraints on UV Metal Line Emission at z~6-9: The Nature of Lyman-alpha Emitting Galaxies in the Reionization-Era

Recent studies have revealed intense UV metal emission lines in a modest sample of z>7 Lyman-alpha emitters, indicating a hard ionizing spectrum is present. If such high ionization features are shown to be common, it may indicate that extreme radiation fields play a role in regulating the visibility of Lyman-alpha in the reionization era. Here we present deep near-infrared spectra of seven galaxies with Lyman-alpha emission at 5.4<z<8.7 (including a newly-confirmed lensed galaxy at z=6.031) and three bright z>7 photometric targets. In nine sources we do not detect UV metal lines. However in the z=8.683 galaxy EGSY8p7, we detect a 4.6 sigma emission line in the narrow spectral window expected for NV 1243. The feature is unresolved (FWHM<90 km/s) and is likely nebular in origin. A deep H-band spectrum of EGSY8p7 reveals non-detections of CIV, He II, and OIII]. The presence of NV requires a substantial flux of photons above 77 eV, pointing to a hard ionizing spectrum powered by an AGN or fast radiative shocks. Regardless of its origin, the intense radiation field of EGSY8p7 may aid the transmission of Lyman-alpha through what is likely a partially neutral IGM. With this new detection, five of thirteen known Lyman-alpha emitters at z>7 have now been shown to have intense UV line emission, suggesting that extreme radiation fields are commonplace among the Lyman-alpha population. Future observations with JWST will eventually clarify the origin of these features and explain their role in the visibility of Lyman-alpha in the reionization era.Comment: 15 pages, 3 tables and 7 figures. Accepted for publication in MNRA

Spectroscopic detection of CIV in a galaxy at z=7.045: Implications for the ionizing spectra of reionization-era galaxies

We present Keck/MOSFIRE observations of UV metal lines in four bright gravitationally-lensed z~6-8 galaxies behind the cluster Abell 1703. The spectrum of A1703-zd6, a highly-magnified star forming galaxy with a Lyman-alpha redshift of z=7.045, reveals a confident detection of the nebular CIV emission line (unresolved with FWHM < 125 km/s). UV metal lines are not detected in the three other galaxies. At z~2-3, nebular CIV emission is observed in just 1% of UV-selected galaxies. The presence of strong CIV emission in one of the small sample of galaxies targeted in this paper may indicate hard ionizing spectra are more common at z~7. The total estimated equivalent width of the CIV doublet (38 A) and CIV/Lyman-alpha flux ratio (0.3) are comparable to measurements of narrow-lined AGNs. Photoionization models show that the nebular CIV line can also be reproduced by a young stellar population, with very hot metal poor stars dominating the photon flux responsible for triply ionizing carbon. Regardless of the origin of the CIV, we show that the ionizing spectrum of A1703-zd6 is different from that of typical galaxies at z~2, producing more H ionizing photons per unit 1500A luminosity and a larger flux density at 30-50 eV. If such extreme radiation fields are typical in UV-selected systems at z>7, it would indicate that reionization-era galaxies are more efficient ionizing agents than previously thought. Alternatively, we suggest that the small sample of Lyman-alpha emitters at z>7 may trace a rare population with intense radiation fields capable of ionizing their surrounding hydrogen distribution. Additional constraints on high ionization emission lines in galaxies with and without Lyman-alpha detections will help clarify whether hard ionizing spectra are common in the reionization era.Comment: 11 pages, 6 figures, submitted to MNRA

The [OIII]λ5007\lambda5007 equivalent width distribution at z ∼2\sim2: The redshift evolution of the extreme emission line galaxies

We determine the [OIII]$\lambda5007$ equivalent width (EW) distribution of $1.700<\rm{z}<2.274$ rest-frame UV-selected (M$_{\rm{UV}}<-19$) star-forming galaxies in the GOODS North and South fields. We make use of deep HDUV broadband photometry catalogues for selection and 3D-HST WFC3/IR grism spectra for measurement of line properties. The [OIII]$\lambda5007$ EW distribution allows us to measure the abundance of extreme emission line galaxies (EELGs) within this population. We model a log-normal distribution to the [OIII]$\lambda5007$ rest-frame equivalent widths of galaxies in our sample, with location parameter $\mu=4.24\pm0.07$ and variance parameter $\sigma= 1.33\pm0.06$. This EW distribution has a mean [OIII]$\lambda5007$ EW of 168$\pm1\r{A}$. The fractions of $\rm{z}\sim2$ rest-UV-selected galaxies with [OIII]$\lambda5007$ EWs greater than $500, 750$ and $1000\r{A}$ are measured to be $6.8^{+1.0}_{-0.9}\%$, $3.6^{+0.7}_{-0.6}\%$, and $2.2^{+0.5}_{-0.4}\%$ respectively. The EELG fractions do not vary strongly with UV luminosity in the range ($-21.6<M_{\rm{UV}}<-19.0$) considered in this paper, consistent with findings at higher redshifts. We compare our results to $\rm{z}\sim5$ and $\rm{z}\sim7$ studies where candidate EELGs have been discovered through Spitzer/IRAC colours, and we identify rapid evolution with redshift in the fraction of star-forming galaxies observed in an extreme emission line phase (a rise by a factor $\sim10$ between $\rm{z}\sim2$ and $\rm{z}\sim7$). This evolution is consistent with an increased incidence of strong bursts in the galaxy population of the reionisation era. While this population makes a sub-dominant contribution of the ionising emissivity at $\rm{z}\simeq2$, EELGs are likely to dominate the ionising output in the reionisation era.Comment: Submitted to MNRAS. 13 pages, 6 figure

Lyman-alpha and CIII] Emission in z=7-9 Galaxies: Accelerated Reionization Around Luminous Star Forming Systems?

We discuss new Keck/MOSFIRE spectroscopic observations of four luminous galaxies at z~7-9 selected to have intense optical line emission by Roberts-Borsani et al. (2016). Previous follow-up has revealed Lyman-alpha in two of the four galaxies. Our new MOSFIRE observations confirm that Lyman-alpha is present in the entire sample. We detect Lyman-alpha emission in COS-zs7-1, confirming its redshift as z=7.154, and we detect Lyman-alpha in EGS-zs8-2 at z=7.477, verifying a tentative detection presented in an earlier study. The ubiquity of Lyman-alpha in this sample is puzzling given that the IGM is likely significantly neutral over 7<z<9. To investigate this result in more detail, we have initiated a campaign to target UV metal emission in the four Lyman-alpha emitters as a probe of both the radiation field and the velocity offset of Lyman-alpha. Here we present the detection of intense CIII] emission in EGS-zs8-1, a galaxy from this sample previously shown to have Lyman-alpha at z=7.73. Photoionization models indicate that an intense radiation field and low metallicity are required to reproduce the intense CIII] and optical line emission. We argue that this extreme radiation field is likely to affect the local environment, increasing the transmission of Lyman-alpha through the galaxy. Moreover, the centroid of CIII] indicates that Lyman-alpha is redshifted from the systemic value by 340 km/s. This velocity offset is larger than that seen in less luminous systems, providing an additional explanation for the transmission of Lyman-alpha emission through the IGM. Since the transmission is further enhanced by the likelihood that such systems are also situated in the densest regions with the largest ionized bubbles, the visibility of Lyman-alpha at z>7 is expected to be strongly luminosity-dependent, with the most effective transmission occurring in systems with intense star formation.Comment: Submitted to MNRAS, 13 pages, 8 figure

Searching for z > 6.5 Analogs Near the Peak of Cosmic Star Formation

Strong [OIII]$\lambda\lambda$4959,5007+H$\beta$ emission appears to be typical in star-forming galaxies at z > 6.5. As likely contributors to cosmic reionization, these galaxies and the physical conditions within them are of great interest. At z > 6.5, where Ly$\alpha$ is greatly attenuated by the intergalactic medium, rest-UV metal emission lines provide an alternative measure of redshift and also constraints on the physical properties of star-forming regions and massive stars. We present the first statistical sample of rest-UV line measurements in z $\sim$ 2 galaxies selected as analogs of those in the reionization era based on [OIII]$\lambda\lambda$4959,5007 EW or rest-frame U-B color. Our sample is drawn from the 3D-HST Survey and spans the redshift range 1.36 $\leqslant$ z $\leqslant$ 2.49. We find that the median Ly$\alpha$ and CIII]$\lambda\lambda$1907,1909 EWs of our sample are significantly greater than those of z $\sim$ 2 UV-continuum-selected star-forming galaxies. Measurements from both individual and composite spectra indicate a monotonic, positive correlation between CIII] and [OIII], while a lack of trend is observed between Ly$\alpha$ and [OIII] at [OIII] EW < 1000\unicode{x212B}. At higher [OIII] EW, extreme Ly$\alpha$ emission starts to emerge. Using stacked spectra, we find that Ly$\alpha$ and CIII] are significantly enhanced in galaxies with lower metallicity. Two objects in our sample appear comparable to z > 6.5 galaxies with exceptionally strong rest-UV metal line emission. These objects have significant CIV$\lambda\lambda$1548,1550, HeII$\lambda$1640, and OIII]$\lambda\lambda$1661,1665 emission in addition to intense Ly$\alpha$ or CIII]. Detailed characterization of these lower-redshift analogs provides unique insights into the physical conditions in z > 6.5 star-forming regions, motivating future observations of reionization-era analogs at lower redshifts.Comment: 22 pages, 9 figures, accepted for publication in Ap

JADES NIRSpec Spectroscopy of GN-z11: Lyman-α\alpha emission and possible enhanced nitrogen abundance in a z=10.60z=10.60 luminous galaxy

We present JADES JWST/NIRSpec spectroscopy of GN-z11, the most luminous candidate $z>10$ Lyman break galaxy in the GOODS-North field with $M_{UV}=-21.5$. We derive a redshift of $z=10.603$ (lower than previous determinations) based on multiple emission lines in our low and medium resolution spectra over $0.8-5.3\,\mu$m. We significantly detect the continuum and measure a blue rest-UV spectral slope of $\beta=-2.4$. Remarkably, we see spatially-extended Lyman-$\alpha$ in emission (despite the highly-neutral IGM expected at this early epoch), offset 555 km/s redward of the systemic redshift. From our measurements of collisionally-excited lines of both low- and high-ionization (including [O II] $\lambda3727$, [Ne III] $\lambda 3869$ and C III] $\lambda1909$) we infer a high ionization parameter ($\log U\sim -2$). We detect the rarely-seen N IV] $\lambda1486$ and N III]$\lambda1748$ lines in both our low and medium resolution spectra, with other high ionization lines seen in low resolution spectrum such as He II (blended with O III]) and C IV (with a possible P-Cygni profile). Based on the observed rest-UV line ratios, we cannot conclusively rule out photoionization from AGN. The high C III]/He II ratios, however, suggest a likely star-formation explanation. If the observed emission lines are powered by star formation, then the strong N III] $\lambda1748$ observed may imply an unusually high $N/O$ abundance. Balmer emission lines (H$\gamma$, H$\delta$) are also detected, and if powered by star formation rather than an AGN we infer a star formation rate of $\sim 20-30 M_{\odot}\,\rm yr^{-1}$ (depending on the IMF) and low dust attenuation. Our NIRSpec spectroscopy confirms that GN-z11 is a remarkable galaxy with extreme properties seen 430 Myr after the Big Bang.Comment: Submitted to Astronomy & Astrophysics, 14 pages, 9 figure

JWST reveals a possible z∼11z \sim 11 galaxy merger in triply-lensed MACS0647−-JD

MACS0647$-$JD is a triply-lensed $z\sim11$ galaxy originally discovered with the Hubble Space Telescope. Here we report new JWST imaging, which clearly resolves MACS0647$-$JD as having two components that are either merging galaxies or stellar complexes within a single galaxy. Both are very small, with stellar masses $\sim10^8\,M_\odot$ and radii $r<100\,\rm pc$. The brighter larger component "A" is intrinsically very blue ($\beta\sim-2.6$), likely due to very recent star formation and no dust, and is spatially extended with an effective radius $\sim70\,\rm pc$. The smaller component "B" appears redder ($\beta\sim-2$), likely because it is older ($100-200\,\rm Myr$) with mild dust extinction ($A_V\sim0.1\,\rm mag$), and a smaller radius $\sim20\,\rm pc$. We identify galaxies with similar colors in a high-redshift simulation, finding their star formation histories to be out of phase. With an estimated stellar mass ratio of roughly 2:1 and physical projected separation $\sim400\,\rm pc$, we may be witnessing a galaxy merger 400 million years after the Big Bang. We also identify a candidate companion galaxy C $\sim3\,{\rm kpc}$ away, likely destined to merge with galaxies A and B. The combined light from galaxies A+B is magnified by factors of $\sim$8, 5, and 2 in three lensed images JD1, 2, and 3 with F356W fluxes $\sim322$, $203$, $86\,\rm nJy$ (AB mag 25.1, 25.6, 26.6). MACS0647$-$JD is significantly brighter than other galaxies recently discovered at similar redshifts with JWST. Without magnification, it would have AB mag 27.3 ($M_{UV}=-20.4$). With a high confidence level, we obtain a photometric redshift of $z=10.6\pm0.3$ based on photometry measured in 6 NIRCam filters spanning $1-5\rm\mu m$, out to $4300\,\r{A}$ rest-frame. JWST NIRSpec observations planned for January 2023 will deliver a spectroscopic redshift and a more detailed study of the physical properties of MACS0647$-$JD.Comment: 27 pages, 14 figures, submitted to Natur

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data