Pharmacological activation of a novel p53-dependent S-phase checkpoint involving CHK-1.

We have recently shown that induction of the p53 tumour suppressor protein by the small-molecule RITA (reactivation of p53 and induction of tumour cell apoptosis; 2,5-bis(5-hydroxymethyl-2-thienyl)furan) inhibits hypoxia-inducible factor-1α and vascular endothelial growth factor expression in vivo and induces p53-dependent tumour cell apoptosis in normoxia and hypoxia. Here, we demonstrate that RITA activates the canonical ataxia telangiectasia mutated/ataxia telangiectasia and Rad3-related DNA damage response pathway. Interestingly, phosphorylation of checkpoint kinase (CHK)-1 induced in response to RITA was influenced by p53 status. We found that induction of p53, phosphorylated CHK-1 and γH2AX proteins was significantly increased in S-phase. Furthermore, we found that RITA stalled replication fork elongation, prolonged S-phase progression and induced DNA damage in p53 positive cells. Although CHK-1 knockdown did not significantly affect p53-dependent DNA damage or apoptosis induced by RITA, it did block the ability for DNA integrity to be maintained during the immediate response to RITA. These data reveal the existence of a novel p53-dependent S-phase DNA maintenance checkpoint involving CHK-1

MGit: A Model Versioning and Management System

Models derived from other models are extremely common in machine learning (ML) today. For example, transfer learning is used to create task-specific models from "pre-trained" models through finetuning. This has led to an ecosystem where models are related to each other, sharing structure and often even parameter values. However, it is hard to manage these model derivatives: the storage overhead of storing all derived models quickly becomes onerous, prompting users to get rid of intermediate models that might be useful for further analysis. Additionally, undesired behaviors in models are hard to track down (e.g., is a bug inherited from an upstream model?). In this paper, we propose a model versioning and management system called MGit that makes it easier to store, test, update, and collaborate on model derivatives. MGit introduces a lineage graph that records provenance and versioning information between models, optimizations to efficiently store model parameters, as well as abstractions over this lineage graph that facilitate relevant testing, updating and collaboration functionality. MGit is able to reduce the lineage graph's storage footprint by up to 7x and automatically update downstream models in response to updates to upstream models

Experiências de mediação na Bienal do Mercosul de 2007 a 2015

A presente pesquisa busca analisar ações de mediação dos projetos pedagógicos de cinco edições da Bienal do Mercosul (6ª – 2007, 7ª – 2009, 8ª – 2011, 9ª – 2013 e 10ª – 2015), a partir dos documentos e publicações disponíveis ao público, e utilizando o conceito de experiência segundo Jorge Larrosa como base teórica das questões de mediação. As ações analisadas são: as visitas mediadas que ocorrem dentro dos espaços de exposição, com a presença de um mediador; os materiais educativos que são produzidos e distribuídos aos públicos como forma de atuação fora da instituição de arte e os espaços físicos ocupados pelos programas educativos dentro da expografia das mostras. Com isso procura-se mostrar que a mediação vai além de um processo dialógico que exige um sujeito mediador, mas um processo que se utiliza também de objetos e estruturas para estabelecer uma relação de experiência entre públicos e arte. A escolha do uso de documentações disponíveis vem a mostrar de que formas essas experiências são registradas e quais acessos temos a elas atualmente.The present research aims to analyze actions of mediation of the pedagogical projects of five editions of the Mercosur Biennial (6th - 2007, 7th - 2009, 8th - 2011, 9th - 2013 and 10th - 2015), based on documents and publications available to the public, and using the concept of experience according to Jorge Larrosa as the theoretical basis of mediation questions. The actions analyzed are: the mediated visits that occur within the exhibition spaces, with the presence of a mediator; the educational materials that are produced and distributed to the public as a way of acting outside the art institution and the physical spaces occupied by the educational programs within the expografia of the shows. The purpose of this work is to show that mediation goes beyond a dialogic process that requires a mediating subject, but a process that also uses objects and structures to establish a relationship of experience between public and art. The choice of the use of available documentation comes to show how these experiences are recorded and what accesses we have to them currently

Large, binge-type meals of high fat diet change feeding behaviour and entrain food anticipatory activity in mice

Copyright © 2014. Published by Elsevier Ltd.Peer reviewedPublisher PD

Radial velocities and membership of stars in the old, distant open cluster Berkeley 29

Multi slit spectroscopy at the Telescopio Nazionale Galileo was employed to measure radial velocities for 20 stars in the direction of the old open cluster Berkeley 29, the farthest known in our Galaxy. Membership information was derived for stars along all the red giant branch, in particular near its tip, and on the red clump. The sample of bona-fide cluster members was used to revise the cluster distance to about 15 kpc, on the basis of an empirical comparison with the red clump in open clusters with known distances. A metallicity [Fe/H] = -0.74 +/- 0.18 was also estimated using the colours of spectroscopically confirmed red giant stars.Comment: 7 pages, 5 figures (fig. 1 at low-res, but JPEG version included too), accepted for publication in A&

Fatores de risco para cesárea segundo tipo de serviço

OBJETIVO: Analizar la tasa de cesárea y factores asociados a su ocurrencia según servicio de salud utilizado, público o privado. MÉTODOS: fue realizado estudio transversal entre 1? de enero y 31 de diciembre de 2007 con todas las gestantes que dieron a luz en los hospitales de la ciudad de Rio Grande, Sur de Brasil. Las gestantes fueron entrevistadas, utilizándose un cuestionario testado y pre-codificado con preguntas sociodemográficas, obstétricas y relacionadas al recién nacido. Fueron desarrollados dos modelos de predicción, uno para las gestantes internadas por el Sistema Único de Salud (público) y otro para las gestantes internadas en servicios privados. En cada modelo fue realizado un análisis multivariado utilizando la regresión de Poisson, calculándose las razones de prevalencia y sus respectivos intervalos con 95% de confianza. RESULTADOS: La tasa de cesárea para el grupo público fue 43% mientras que en el grupo privado fue 86%. Los factores sociodemográficos y el parto gemelar fueron más importantes en el modelo público, así como el número de embarazos (reducción de 25% vs. 13% en el grupo privado) y cesárea previa (incremento de 86% vs. 24% en el privado). Consultas de prenatal e internaciones afectaron el resultado solamente en el grupo público. CONCLUSIONES: Las tasas de cesárea fueron elevadas en los dos grupos, siendo el doble en el servicio privado. Los factores de riesgo son diferentes en magnitud, según el tipo de gestante.OBJECTIVE: To analyze the rate of cesarean section and differences in risk factors by category of health service, either public or private. METHODS: A cross-sectional study was carried out including all pregnant women in labor admitted to hospitals in the city of Rio Grande, Southern Brazil, between January 1 and December 31, 2007. A pre-coded and pre-tested questionnaire was used to collect on social, demographic, obstetric and newborn care information. Two regression models were constructed: one for public users and the other one for private ones. Poisson regression was used in each model in the multivariate analysis. Prevalence rates and 95% confidence intervals were calculated for each adjusted factor. RESULTS: The rate of cesarean section was 43% and 86% among public and private users. Sociodemographic factors and twin births have a more significant impact among public users as well as number of pregnancies (25% vs. 13% reduction in public and private users, respectively) and previous cesarean section (86% vs. 24% increase in public and private users, respectively). Prenatal care visits and hospital admissions affected the outcome only in women users of public services. CONCLUSIONS: Cesarean section rates were high in both groups studied, but it was twice as high among women cared in the private sector. Associated factors differ in magnitude by category of service used.OBJETIVO: Analisar a taxa de cesárea e fatores associados à sua ocorrência segundo serviço de saúde utilizado, público ou privado. MÉTODOS: Foi realizado estudo transversal entre 1º de janeiro e 31 de dezembro de 2007 com todas as gestantes que deram à luz nos hospitais da cidade de Rio Grande, RS. As gestantes foram entrevistadas, utilizando-se um questionário testado e pré-codificado com questões sociodemográficas, obstétricas e relacionadas ao recém-nascido. Foram desenvolvidos dois modelos de predição, um para as gestantes internadas pelo Sistema Único de Saúde (público) e outro para as gestantes internadas em serviços privados. Em cada modelo foi realizada uma análise multivariada utilizando a regressão de Poisson, calculando-se as razões de prevalência e seus respectivos intervalos com 95% de confiança. RESULTADOS: A taxa de cesárea para o grupo público foi 43% enquanto no grupo privado foi 86%. Os fatores sociodemográficos e o parto gemelar foram mais importantes no modelo público, assim como o número gravidezes (redução de 25% vs. 13% no grupo privado) e cesárea prévia (incremento de 86% vs. 24% no privado). Consultas de pré-natal e internações afetaram o desfecho somente no grupo público. CONCLUSÕES: As taxas de cesárea foram elevadas nos dois grupos, sendo o dobro no serviço privado. Os fatores de risco são diferentes em magnitude, segundo o tipo de gestante

Ethan Kleinberg: Teoría de la Historia como Fantología: Ethan Kleinberg: Theory of History as Hauntology

Resumen               Ethan Kleinberg, es profesor de Historia y Letras de la Universidad de Wesleyan, director del Centro de Humanidades y editor ejecutivo de la revista History and Theory. Su primer libro, Generation Existential: Heidegger’s Philosophy in France, 1927-1961, fue publicado por la Universidad de Cornell, y galardonado con el Premio Morris D. Forkosch 2006 al mejor libro de historia intelectual otorgado por la revista Journal of the History of Ideas. Recientemente, el profesor Kleinberg co-editó de la mano de Ranjan Ghosh el volumen Presence: Philosophy, History and Cultural Theory for the 21st Century, publicado también por la Universidad de Cornell. Por otro lado, su libro, Haunting History: For a Deconstructive Approach to the Past, fue lanzado en las Series meridianas de la Universidad de Stanford en el otoño de 2017.  Actualmente, se encuentra culminando su libro The Myth of Emmanuel Levinas, centrado en las lecturas Talmúdicas que el filósofo franco-judío presentó en París entre 1960 y 1990. En junio de 2016, tuve la oportunidad de llevar a cabo una entrevista con el profesor Kleinberg, cuando fui un estudiante visitante de investigación en el Centro de Humanidades de la Universidad Wesleyan. Además, aprovechamos la segunda Conferencia Internacional de Teoría de la Historia (2ª INTH), ocurrida en Ouro Preto, Brasil, del 23 de agosto al 26 de agosto, para ampliar la entrevista y para grabar una versión corta. El video se encuentra en el siguiente enlace: https://www.youtube.com/watch?v=PH9q_bJboHs  Palabras clave  Teoría de la Historia; Historia de la Historiografía; Fantología.  Abstract  Ethan Kleinberg is Professor of History and Letters of Wesleyan University. He is the Director of the Center for Humanities and the Editor-in-Chief of History and Theory. His first book, Generation Existential: Heidegger’s Philosophy in France, 1927-1961, published by Cornell University Press, was awarded the 2006 Morris D. Forkosch prize for the best book in intellectual history by the Journal of the History of Ideas. Recently, Professor Kleinberg co-edited with Ranjan Ghosh the volume Presence: Philosophy, History, and Cultural Theory for the 21st Century, published by Cornell University Press as well. His book, Haunting History: For a Deconstructive Approach to the Past, was released in the Meridian Series from Stanford University Press in fall 2017.  He is also finishing The Myth of Emmanuel Levinas, centered on the Talmudic Lectures that the French-Jewish philosopher presented in Paris between 1960 and 1990. I had the opportunity to conduct an interview with Professor Kleinberg in June 2016, when I was a Visiting Student Researcher at the Center for Humanities at Wesleyan University. We also took advantage of the Second International Network for Theory of History Conference (2nd INTH) in Ouro Preto, Brazil, from August 23 to August 26, 2016, to expand the interview and to record a short version. Here it is the link: https://www.youtube.com/watch?v=PH9q_bJboHs Key Words  Theory of History; History of Historiography; Hauntology

Chips de mandioca: efeito do pré-tratamento na absorção de óleo de fritura e na cor do

A cultura da mandioca (Manihot esculenta Crantz) é uma das mais importantes fontes de carboidratos para os consumidores de renda mais baixa em países tropicais, devido ao seu alto teor de amido e sabor suave pode ser empregada em diversos produtos alimentícios prontos para o consumo com maior valor agregado como, por exemplo, chips de mandioca. Diante disso objetivou-se estudar efeito de diferentes pré-tratamentos anterior à fritura, na qualidade física de chips de mandioca. O estudo foi desenvolvido no Laboratório de Análise e Química de Alimentos (LATEC) da Universidade Federal da Grande Dourados. Os pré-tratamentos aplicados foram: 1)fatias lavadas, submetidas ao branqueamento (TT); 2) fatias lavadas, submetidas ao branqueamento e secagem em estufa por dez minutos (TSP); 3) fatias lavadas, submetidas ao branqueamento e secagem em estufa por três horas (TST) e 4) controle (sem pré-tratamento). Após os pré-tratamentos as fatias foram fritas em óleo de soja a 180 °C. Os chips foram analisados quanto ao teor de lipídeos e a cor. Os resultados mostraram que não houve diferença significativa entre os pré-tratamentos no conteúdo de lipídeos determinados nos chips. Porém, a cor do produto submetido aos pré-tratamentos TST e TSP foi mais clara que as amostras controle (C) e com branqueamento (TT)