A Note on the Stability of Exponential Dichotomy of Linear Differential Equations

We present an elementary Functional Analytic proof of the roughness of Exponential Dichotomy of Ordinary Differential Equations (with exponential growth) on an arbitrary Banach Space.Comment: 10 pages, exposed on 5th International Conference "Actualities and Perspectives on Hardware and Software" - APHS2009, Timisoara, Romani

Modular uniform convexity structures and applications to boundary value problems with non-standard growth

We establish the existence and uniqueness of the solution to the Dirichlet problem for the variable exponent $p$-Laplacian on a bounded, smooth domain $\Omega \subset {\mathbb R}^n$, where the boundary datum belongs to $W^{1,p}(\Omega)$. Our analysis considers a continuous and bounded exponent $p$ satisfying $1<\inf\limits_{x\in \Omega}p(x)$ and $\sup\limits_{x\in \Omega}p(x)<\infty$, and is based on the uniform convexity of the Dirichlet integral, which is highly non trivial and in the variable exponent case is not related to the uniform convexity of the Sobolev norm

Boundary Layers on Sobolev–Besov Spaces and Poisson's Equation for the Laplacian in Lipschitz Domains

AbstractWe study inhomogeneous boundary value problems for the Laplacian in arbitrary Lipschitz domains with data in Sobolev–Besov spaces. As such, this is a natural continuation of work in [Jerison and Kenig,J. Funct. Anal.(1995), 16–219] where the inhomogeneous Dirichlet problem is treated via harmonic measure techniques. The novelty of our approach resides in the systematic use of boundary integral methods. In this regard, the key results are establishing the invertibility of the classical layer potential operators on scales of Sobolev–Besov spaces on Lipschitz boundaries for optimal ranges of indices. Applications toLp-based Helmholtz type decompositions of vector fields in Lipschitz domains are also presented

Scale for assessing the quality of Mexican adults' mealtime habits

To construct a scale for assessing the quality of mealtime habits in a sample of urban Mexican adults, computing the contribution of a set of advisable and unadvisable mealtime habits. Material and Methods. We performed an exploratory factor analysis among 7 472 adults participating in the baseline assessment of the Health Workers Cohort Study, to assess the mealtime habits quality. Likelihood ratio test for difference of two probabilities and test for the difference of two means were used to identify differences between low and high categories of the Mealtime Habits Quality Scale (MHQS) across variables of interest. Results. Participants with the top quality of mealtime habits showed lower rates of overweight, obesity, abdominal obesity, and elevated body fat. They were also more adherent to a prudent dietary pattern than a western dietary pattern, and consumed more fruits and vegetables. Conclusions. Anthropometric and dietary variables differed across MHQS categories. However, further validation of the scale, and assessment of their ability to predict weight gain or related diseases are needed, using prospective and intervention studies

Sensitivity of tumor cells towards CIGB-300 anticancer peptide relies on its nucleolar localization

CIGB-300 is a novel anticancer peptide that impairs the casein kinase 2-mediated phosphorylation by direct binding to the conserved phosphoacceptor site on their substrates. Previous findings indicated that CIGB-300 inhibits tumor cell proliferation in vitro and induces tumor growth delay in vivo in cancer animal models. Interestingly, we had previously demonstrated that the putative oncogene B23/nucleophosmin (NPM) is the major intracellular target for CIGB-300 in a sensitive human lung cancer cell line. However, the ability of this peptide to target B23/NPM in cancer cells with differential CIGB-300 response phenotype remained to be determined. Interestingly, in this work, we evidenced that CIGB-300's antiproliferative activity on tumor cells strongly correlates with its nucleolar localization, the main subcellular localization of the previously identified B23/NPM target. Likewise, using CIGB-300 equipotent doses (concentration that inhibits 50% of proliferation), we demonstrated that this peptide interacts and inhibits B23/NPM phosphorylation in different cancer cell lines as evidenced by in vivo pull-down and metabolic labeling experiments. Moreover, such inhibition was followed by a fast apoptosis on CIGB-300-treated cells and also an impairment of cell cycle progression mainly after 5 h of treatment. Altogether, our data not only validates B23/NPM as a main target for CIGB-300 in cancer cells but also provides the first experimental clues to explain their differential antiproliferative response. Importantly, our findings suggest that further improvements to this cell penetrating peptide-based drug should entail its more efficient intracellular delivery at such subcellular localization.Fil: Perera, Yasser. Centro de Ingeniería Genética y Biotecnología; CubaFil: Costales, Heydi C.. Centro de Ingeniería Genética y Biotecnología; CubaFil: Diaz, Yakelin. Centro de Ingeniería Genética y Biotecnología; CubaFil: Reyes, Osvaldo. Centro de Ingeniería Genética y Biotecnología; CubaFil: Farina, Hernán Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mendez, Lissandra. Centro de Ingeniería Genética y Biotecnología; CubaFil: Gómez, Roberto E.. Centro de Ingeniería Genética y Biotecnología; CubaFil: Acevedo, Boris E.. Centro de Ingeniería Genética y Biotecnología; CubaFil: Gomez, Daniel Eduardo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perea, Silvio E.. Centro de Ingeniería Genética y Biotecnología; Cub

3D chromatin connectivity underlies replication origin efficiency in mouse embryonic stem cells

In mammalian cells, chromosomal replication starts at thousands of origins at which replisomes are assembled. Replicative stress triggers additional initiation events from 'dormant' origins whose genomic distribution and regulation are not well understood. In this study, we have analyzed origin activity in mouse embryonic stem cells in the absence or presence of mild replicative stress induced by aphidicolin, a DNA polymerase inhibitor, or by deregulation of origin licensing factor CDC6. In both cases, we observe that the majority of stress-responsive origins are also active in a small fraction of the cell population in a normal S phase, and stress increases their frequency of activation. In a search for the molecular determinants of origin efficiency, we compared the genetic and epigenetic features of origins displaying different levels of activation, and integrated their genomic positions in three-dimensional chromatin interaction networks derived from high-depth Hi-C and promoter-capture Hi-C data. We report that origin efficiency is directly proportional to the proximity to transcriptional start sites and to the number of contacts established between origin-containing chromatin fragments, supporting the organization of origins in higher-level DNA replication factories.MCIN/AEI/10.13039/501100011033 [BFU2016-80402-R and PID2019-106707RB-100 to JM; BFU2016-78849-P and PID2019-105949GB-I00 to MG]; ‘ERDF A way of making Europe’; ‘CNIO Friends’ postdoctoral fellowship (to V.P.); Fondation Toulouse Cancer Santé and the Pierre Fabre Research Institute as part of the Chair of Bioinformatics in Oncology of the CRCT; CNIO-La Caixa predoctoral fellowships (to K.J., M.R.); Portuguese Foundation for Science and Technology [FCT-SFRH/BD/81027/2011 to R.A.]; Spanish Ministry of Science and Innovation [BES-2014–070050 to J.M.F.-J.]; Foundation for Polish Science co-financed by the European Union ERFD funds [TEAM/2016–3/30 to K.J.]; Polish National Science Centre [2020/37/B/NZ2/03757 to K.J.). Funding for open access charge: Spanish Ministry of Science and Innovation (PID2019-106707RB-100)

Biomimetic biosensor based on lipidic layers containing tyrosinase and lutetium bisphthalocyanine for the detection of antioxidants

This paper describes the preparation of a biomimetic Langmuir-Blodgett film of tyrosinase incorporated in a lipidic layer and the use of lutetium bisphthalocyanine as an electron mediator for the voltammetric detection of phenol derivatives, which include one monophenol (vanillic acid), two diphenols (catechol and caffeic acid) and two triphenols (gallic acid and pyrogallol). The first redox process of the voltammetric responses is associated with the reduction of the enzymatically formed o-quinone and is favoured by the lutetium bisphthalocyanine because significant signal amplification is observed, while the second is associated with the electrochemical oxidation of the antioxidant and occurs at lower potentials in the presence of an electron mediator. The biosensor shows low detection limit (1.98 × 10-6 - 27.49 × 10-6 M), good reproducibility, and high affinity to antioxidants (KM in the range of 62.31-144.87 μM).\ud The excellent functionality of the enzyme obtained using a biomimetic immobilisation method, the selectivity afforded by enzyme catalysis, the signal enhancement caused by the lutetium bisphthalocyanine mediator and the increased selectivity of the curves due to the occurrence of two redox processes make these sensors exceptionally suitable for the detection of phenolic compounds.MICINN (AGL2009-12660/ALI)FAPESPCNPqCAPE

Pre-Existing Tumoral B Cell Infiltration and Impaired Genome Maintenance Correlate with Response to Chemoradiotherapy in Locally Advanced Rectal Cancer

Locally advanced rectal cancer (LARC) remains a medical challenge. Reliable biomarkers to predict which patients will significantly respond to neoadjuvant chemoradiotherapy (nCRT) have not been identified. We evaluated baseline genomic and transcriptomic features to detect differences that may help predict response to nCRT. Eligible LARC patients received nCRT (3D-LCRT 50.4 Gy plus capecitabine 825 mg/m2/bid), preceded by three cycles of CAPOX in high systemic-relapse risk tumors, and subsequent surgery. Frozen tumor biopsies at diagnosis were sequenced using a colorectal cancer panel. Transcriptomic data was used for pathway and cell deconvolution inferential algorithms, coupled with immunohistochemical validation. Clinical and molecular data were analyzed according to nCRT outcome. Pathways related to DNA repair and proliferation (p RAS and TP53 mutations (p = 0.001) were associated with poor response. Enrichment of expression signatures related to enhanced immune response, particularly B cells and interferon signaling (p RAS and TP53 mutations along with a proficient DNA repair system that may counteract chemoradio-induced DNA damage was associated with poor response.Facultad de Ciencias MédicasCentro de Investigaciones Inmunológicas Básicas y Aplicada

Programa Nacional de Ecorregiones. La región Patagonia : Centros Regionales Patagonia Norte y Patagonia Sur

La Ecorregión Patagónica es una vasta región (780.000 km2), que cubre un amplio rango latitudinal (36º a 55º S), limitada al oeste (O) por la Cordillera de los Andes y al este (E) por el Océano Atlántico y se extiende desde el curso de los ríos Barrancas – Colorado hasta el Canal de Beagle. Abarca la totalidad del territorio de las provincias de Neuquén, Río Negro, Chubut, Santa Cruz y Tierra del Fuego e Islas del Atlántico Sur.Estación Experimental Agropecuaria BarilocheFil: Mendez Casariego, Hugo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area de Desarrollo Rural; ArgentinaFil: Bran, Donaldo Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area de Recursos Naturales; ArgentinaFil: Peralta, Carlos Ramiro. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area de Desarrollo Rural; ArgentinaFil: Madariaga, Marta Cecilia. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area de Desarrollo Rural; ArgentinaFil: Huerta, Guillermo Jose. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area de Desarrollo Rural; ArgentinaFil: Villarreal, Patricia. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Alto Valle; ArgentinaFil: Oliva, Gabriel Esteban. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Santa Cruz; ArgentinaFil: Lopez Raggi, Facundo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Centro Regional Patagonia Norte; ArgentinaFil: Santagni, Adalberto. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Alto Valle; ArgentinaFil: Easdale, Marcos Horacio. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Recursos Naturales; ArgentinaFil: Sarmiento, Adolfo Carlos. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area de Desarrollo Rural; ArgentinaFil: Giraudo, Celso Gabriel. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche; ArgentinaFil: Rial, Pablo Eduardo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Santa Cruz; ArgentinaFil: Escobar, Juan Maria. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Trelew; ArgentinaFil: Marciani, Santiago Nicolas. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area de Desarrollo Rural; ArgentinaFil: Martinez, Roberto Simon. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Valle Inferior; ArgentinaFil: Elissalde, Nestor Osvaldo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Trelew; Argentin

Nestin-GFP Transgene Reveals Neural Precursor Cells in Adult Skeletal Muscle

Background: Therapy for neural lesions or degenerative diseases relies mainly on finding transplantable active precursor cells. Identifying them in peripheral tissues accessible for biopsy, outside the central nervous system, would circumvent the serious immunological and ethical concerns impeding cell therapy. Methodology/Principal Findings: In this study, we isolated neural progenitor cells in cultured adult skeletal muscle from transgenic mice in which nestin regulatory elements control GFP expression. These cells also expressed the early neural marker Tuj1 and light and heavy neurofilament but not S100b, indicating that they express typical neural but not Schwann cell markers. GFP+/Tuj1+ cells were also negative for the endothelial and pericyte markers CD31 and a-smooth muscle actin, respectively. We established their a) functional response to glutamate in patch-clamp recordings; b) interstitial mesenchymal origin; c) replicative capacity; and d) the environment necessary for their survival after fluorescenceactivated cell sorting. Conclusions/Significance: We propose that the decline in nestin-GFP expression in muscle progenitor cells and its persistence in neural precursor cells in muscle cultures provide an invaluable tool for isolating a population of predifferentiated neural cells with therapeutic potential