The effect of complex dispersion and characteristic impedance on the gain of superconducting traveling-wave kinetic inductance parametric amplifiers

Superconducting traveling-wave parametric amplifiers are a promising amplification technology suitable for applications in submillimeter astronomy. Their implementation relies on the use of Floquet transmission lines in order to create strong stopbands to suppress undesired harmonics. In the design process, amplitude equations are used to predict their gain, operation frequency, and bandwidth. However, usual amplitude equations do not take into account the real and imaginary parts of the dispersion and characteristic impedance that results from the use of Floquet lines, hindering reliable design. In order to overcome this limitation, we have used the multiple-scales method to include those effects. We demonstrate that complex dispersion and characteristic impedance have a stark effect on the transmission line's gain, even suppressing it completely in certain cases. The equations presented here can, thus, guide to a better design and understanding of the properties of this kind of amplifiers.Comment: 7 pages, 7 figures, submitted to IEEE Transactions on Applied Superconductivit

Control of deviations and prediction of surface roughness from micro machining of THz waveguides using acoustic emission signals

By using acoustic emission (AE) it is possible to control deviations and surface quality during micro milling operations. The method of micro milling is used to manufacture a submillimetre waveguide where micro machining is employed to achieve the required superior finish and geometrical tolerances. Submillimetre waveguide technology is used in deep space signal retrieval where highest detection efficiencies are needed and therefore every possible signal loss in the receiver has to be avoided and stringent tolerances achieved. With a sub-standard surface finish the signals travelling along the waveguides dissipate away faster than with perfect surfaces where the residual roughness becomes comparable with the electromagnetic skin depth. Therefore, the higher the radio frequency the more critical this becomes. The method of time-frequency analysis (STFT) is used to transfer raw AE into more meaningful salient signal features (SF). This information was then correlated against the measured geometrical deviations and, the onset of catastrophic tool wear. Such deviations can be offset from different AE signals (different deviations from subsequent tests) and feedback for a final spring cut ensuring the geometrical accuracies are met. Geometrical differences can impact on the required transfer of AE signals (change in cut off frequencies and diminished SNR at the interface) and therefore errors have to be minimised to within 1 µm. Rules based on both Classification and Regression Trees (CART) and Neural Networks (NN) were used to implement a simulation displaying how such a control regime could be used as a real time controller, be it corrective measures (via spring cuts) over several initial machining passes or, with a micron cut introducing a level plain measure for allowing setup corrective measures (similar to a spirit level)

Simulating the detection of the global 21 cm signal with MIST for different models of the soil and beam directivity

The Mapper of the IGM Spin Temperature (MIST) is a new ground-based, single-antenna, radio experiment attempting to detect the global 21 cm signal from the Dark Ages and Cosmic Dawn. A significant challenge in this measurement is the frequency-dependence, or chromaticity, of the antenna beam directivity. MIST observes with the antenna above the soil and without a metal ground plane, and the beam directivity is sensitive to the electrical characteristics of the soil. In this paper, we use simulated observations with MIST to study how the detection of the global 21 cm signal from Cosmic Dawn is affected by the soil and the MIST beam directivity. We simulate observations using electromagnetic models of the directivity computed for single- and two-layer models of the soil. We test the recovery of the Cosmic Dawn signal with and without beam chromaticity correction applied to the simulated data. We find that our single-layer soil models enable a straightforward recovery of the signal even without chromaticity correction. Two-layer models increase the beam chromaticity and make the recovery more challenging. However, for the model in which the bottom soil layer has a lower electrical conductivity than the top layer, the signal can be recovered even without chromaticity correction. For the other two-layer models, chromaticity correction is necessary for the recovery of the signal and the accuracy requirements for the soil parameters vary between models. These results will be used as a guideline to select observation sites that are favorable for the detection of the Cosmic Dawn signal.Comment: Accepted for publication in the Astrophysical Journa

YopJ-Induced Caspase-1 Activation in Yersinia-Infected Macrophages: Independent of Apoptosis, Linked to Necrosis, Dispensable for Innate Host Defense

Yersinia outer protein J (YopJ) is a type III secretion system (T3SS) effector of pathogenic Yersinia (Yersinia pestis, Yersinia enterocolitica and Yersinia pseudotuberculosis) that is secreted into host cells. YopJ inhibits survival response pathways in macrophages, causing cell death. Allelic variation of YopJ is responsible for differential cytotoxicity in Yersinia strains. YopJ isoforms in Y. enterocolitica O:8 (YopP) and Y. pestis KIM (YopJKIM) strains have high cytotoxic activity. In addition, YopJKIM-induced macrophage death is associated with caspase-1 activation and interleukin-1β (IL-1β secretion. Here, the mechanism of YopJKIM-induced cell death, caspase-1 activation, and IL-1β secretion in primary murine macrophages was examined. Caspase-3/7 activity was low and the caspase-3 substrate poly (ADP-ribose) polymerase (PARP) was not cleaved in Y. pestis KIM5-infected macrophages. In addition, cytotoxicity and IL-1β secretion were not reduced in the presence of a caspase-8 inhibitor, or in B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax)/Bcl-2 homologous antagonist/killer (Bak) knockout macrophages, showing that YopJKIM-mediated cell death and caspase-1 activation occur independent of mitochondrial-directed apoptosis. KIM5-infected macrophages released high mobility group protein B1 (HMGB1), a marker of necrosis, and microscopic analysis revealed that necrotic cells contained active caspase-1, indicating that caspase-1 activation is associated with necrosis. Inhibitor studies showed that receptor interacting protein 1 (RIP1) kinase and reactive oxygen species (ROS) were not required for cytotoxicity or IL-β release in KIM5-infected macrophages. IL-1β secretion was reduced in the presence of cathepsin B inhibitors, suggesting that activation of caspase-1 requires cathepsin B activity. Ectopically-expressed YopP caused higher cytotoxicity and secretion of IL-1β in Y. pseudotuberculosis-infected macrophages than YopJKIM. Wild-type and congenic caspase 1 knockout C57BL/6 mice were equally susceptible to lethal infection with Y. pseudotuberculosis ectopically expressing YopP. These data suggest that YopJ-induced caspase-1 activation in Yersinia-infected macrophages is a downstream consequence of necrotic cell death and is dispensable for innate host resistance to a strain with enhanced cytotoxicity

DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis

Fascioliasis is a highly pathogenic zoonotic disease emerging in recent decades, in part due to the effects of climate and global changes. South America is the continent presenting more numerous human fascioliasis endemic areas and the highest Fasciola hepatica infection prevalences and intensities known in humans. These serious public health scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks. To understand this difference, we characterized F. hepatica from cattle and horses and lymnaeids of Uruguay by sequencing of ribosomal DNA ITS-2 and ITS-1 spacers and mitochondrial DNA cox1, nad1 and 16S genes. Results indicate that vectors belong to Lymnaea neotropica instead of to Lymnaea viator, as always reported from Uruguay. Our correlation of fasciolid and lymnaeid haplotypes with historical data on the introduction and spread of livestock species into Uruguay allow to understand the molecular diversity detected. We study the life cycle and transmission features of F. hepatica by L. neotropica of Uruguay under standardized experimental conditions to enable a comparison with the transmission capacity of F. hepatica by Galba truncatula at very high altitude in Bolivia. Results demonstrate that although L. neotropica is a highly efficient vector in the lowlands, its transmission capacity is markedly lower than that of G. truncatula in the highlands. On this baseline, we review the human fascioliasis cases reported in Uruguay and analyze the present and future risk of human infection in front of future climate change estimations

A high-performance 650GHz sideband-separating mixer -Design and results

We designed, built and tested a new sidebandseparating mixer assembly for the 600-720 GHz band (ALMA Band 9). By concentrating on the input matching and isolation of the quadrature hybrid and associated waveguide components, rather than on the phase and amplitude balance, we minimized standing waves and especially asymmetric reflection paths, which are highly detrimental to the image rejection ratio (IRR). IRRs in excess of 15 dB are obtained repeatably with different blocks and mixer pairs. At the same time, the SSB noise temperature is increased by not more than 20-30K with respect to the bare mixer devices, corresponding to a loss of about 0.5 dB in the waveguide structure. A considerable contribution to the IRR are reflections in the IF system. If these are eliminated, i.e., by using highly matched IF amplifiers, we expect worst-case IRRs of 18 dB or better can be reached, even in array configurations. In less demanding cases, the ample margin in IRR on the RF side can be used to build a system with reasonably matched amplifiers that still meets a typical 10 dB IRR specification. These 2SB mixers are intended for future sideband-separating receivers on the APEX (Chile) and LLAMA (Argentina) observatories and for deployment in any other observatory that would benefit from sideband separation in the 600-720 GHz band