89 research outputs found
Motivação e clima organizacional: um estudo de caso em uma Instituição Pública Federal de Ensino Superior do DF
Esse trabalho tem como objetivo pesquisar a influência do clima organizacional na motivação dos colaboradores de um dos setores de uma Instituição Pública Federal de Ensino Superior do Distrito Federal. Trata-se de uma pesquisa de natureza descritiva, cujas técnicas de coleta de dados compreendem um levantamento bibliográfico e uma pesquisa de campo em uma organização pública do segmento educacional, com vistas a entender como determinados elementos do clima da organização podem influenciar a motivação dos colaboradores inseridos no contexto da instituição. Os achados mais importantes do estudo são os resultados positivos dos fatores que mensuram o clima, dentre eles a clareza organizacional, reconhecimento, condições físicas e materiais, trabalho em equipe, relacionamento interpessoal, conflitos, rotatividade, produtividade, liderança e avaliação de desempenho, e ainda a relação afirmativa da influência do clima na motivação dos colaboradores
The Health and Future of the Six Hare Species in Europe: A Closer Look at the Iberian Hare
Although there are around 40 species of hares in the world divided into three different genera (Lepus, Caprolagus, and Pronolagus), only six species inhabit Europe, all belonging to genus Lepus. The conservation status of these six species was recently revised in the International Conservation Union (IUCN) Red List of Threatened Species. Lepus castroviejoi and L. corsicanus were attributed the status of “vulnerable”. The other four species, L. europaeus, L. timidus, L. capensis, and L. granatensis, were considered of “least concern” although a declining trend was recognized for the last two species’ wild populations. Here we review the major threats to the hare species in Europe, with emphasis on infectious diseases. Furthermore, we present the sanitary data regarding the Iberian hare populations from Portugal, which were severely affected by the emergence of a naturally occurring recombinant myxoma virus (MYXV), first reported in mid-2018. The recent detection in 2019 of a leporid herpesvirus (LeHV-5), which pathogenicity appears to be exacerbated in MYXV-infected hares, brings additional concerns to the health and conservation of the Iberian hare
A COVID-19 outbreak in a rheumatology department upon the early days of the pandemic
Copyright © 2020 Cascão, Vidal, Carvalho, Lopes, Romão, Goncalves, Moita andFonseca. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Objectives: To describe our experience with a coronavirus disease 2019 (COVID-19) outbreak within a large rheumatology department early in the pandemic. Methods: Symptomatic and asymptomatic healthcare workers (HCWs) had a naso-oropharyngeal swab for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and were followed clinically. Reverse transcription polymerase-chain reaction (RT-PCR) was repeated to document cure, and serological response was assessed. Patients with risk contacts within the department in the 14 days preceding the outbreak were screened for COVID-19 symptoms. Results: 14/34 HCWs (41%; 40 ± 14 years, 71% female) tested positive for SARS-CoV-2, and 11/34 (32%) developed symptoms but were RT-PCR-negative. Half of RT-PCR-positive HCWs did not report fever, cough, or dyspnea before testing, which were absent in 3/14 cases (21%). Mild disease prevailed (79%), but 3 HCWs had moderate disease requiring further assessment, which excluded severe complications. Nevertheless, symptom duration (28 ± 18 days), viral shedding (31 ± 10 days post-symptom onset, range 15-51), and work absence (29 ± 28 days) were prolonged. 13/14 (93%) of RT-PCR-positive and none of the RT-PCR-negative HCWs had a positive humoral response Higher IgG indexes were observed in individuals over 50 years of age (14.5 ± 7.7 vs. 5.0 ± 4.4, p = 0.012). Of 617 rheumatic patients, 8 (1.3%) developed COVID-19 symptoms (1/8 hospitalization, 8/8 complete recovery), following a consultation/procedure with an asymptomatic (7/8) or mildly symptomatic (1/8) HCW. Conclusions: A COVID-19 outbreak can occur among HCWs and rheumatic patients, swiftly spreading over the presymptomatic stage. Mild disease without typical symptoms should be recognized and may evolve with delayed viral shedding, prolonged recovery, and adequate immune response in most individuals.info:eu-repo/semantics/publishedVersio
Schinus terebinthifolius leaf extract causes midgut damage, interfering with survival and development of Aedes aegypti larvae
In this study, a leaf extract from Schinus terebinthifolius was evaluated for effects on survival, development, and midgut of A. aegypti fourth instar larvae (L4), as well as for toxic effect on Artemia salina. Leaf extract was obtained using 0.15 M NaCl and evaluated for phytochemical composition and lectin activity. Early L4 larvae were incubated with the extract (0.3–1.35%, w/v) for 8 days, in presence or absence of food. Polymeric proanthocyanidins, hydrolysable tannins, heterosid and aglycone flavonoids, cinnamic acid derivatives, traces of steroids, and lectin activity were detected in the extract, which killed the larvae at an LC50 of 0.62% (unfed larvae) and 1.03% (fed larvae). Further, the larvae incubated with the extract reacted by eliminating the gut content. No larvae reached the pupal stage in treatments at concentrations between 0.5% and 1.35%, while in the control (fed larvae), 61.7% of individuals emerged as adults. The extract (1.0%) promoted intense disorganization of larval midgut epithelium, including deformation and hypertrophy of cells, disruption of microvilli, and vacuolization of cytoplasms, affecting digestive, enteroendocrine, regenerative, and proliferating cells. In addition, cells with fragmented DNA were observed. Separation of extract components by solid phase extraction revealed that cinnamic acid derivatives and flavonoids are involved in larvicidal effect of the extract, being the first most efficient in a short time after larvae treatment. The lectin present in the extract was isolated, but did not show deleterious effects on larvae. The extract and cinnamic acid derivatives were toxic to A. salina nauplii, while the flavonoids showed low toxicity. S. terebinthifolius leaf extract caused damage to the midgut of A. aegypti larvae, interfering with survival and development. The larvicidal effect of the extract can be attributed to cinnamic acid derivatives and flavonoids. The data obtained using A. salina indicates that caution should be used when employing this extract as a larvicidal agent
Candida albicans PROTEIN PROFILE CHANGES IN RESPONSE TO THE BUTANOLIC EXTRACT OF Sapindus saponariaL.
Candida albicans is an opportunistic human pathogen that is capable of causing superficial and systemic infections in immunocompromised patients. Extracts of Sapindus saponaria have been used as antimicrobial agents against various organisms. In the present study, we used a combination of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify the changes in protein abundance of C. albicans after exposure to the minimal inhibitory concentration (MIC) and sub-minimal inhibitory concentration (sub-MIC) of the butanolic extract (BUTE) of S. saponaria and also to fluconazole. A total of six different proteins with greater than 1.5 fold induction or repression relative to the untreated control cells were identified among the three treatments. In general, proteins/enzymes involved with the glycolysis (GPM1, ENO1, FBA1), amino acid metabolism (ILV5, PDC11) and protein synthesis (ASC1) pathways were detected. In conclusion, our findings reveal antifungal-induced changes in protein abundance of C. albicans. By using the previously identified components of the BUTE of S. saponaria(e.g., saponins and sesquiterpene oligoglycosides), it will be possible to compare the behavior of compounds with unknown mechanisms of action, and this knowledge will help to focus the subsequent biochemical work aimed at defining the effects of these compounds
Candida albicans PROTEIN PROFILE CHANGES IN RESPONSE TO THE BUTANOLIC EXTRACT OF Sapindus saponaria L.
Effects of conversion of riparian forest to pasture on soil physical and hydraulic properties
Os efeitos da mudança de uso da terra (MUT) sobre as propriedades físico-hídricas do solo ainda precisam ser devidamente compreendidos no âmbito da bacia hidrográfica do rio Araguaia. Em muitos casos, a MUT ocorre em ambientes ripários, o que potencializa seu impacto nos ecossistemas terrestres e aquáticos. Diante disso, o objetivo do estudo foi avaliar os efeitos da MUT nas propriedades físico-hídricos do solo de uma pastagem (anteriormente floresta ripária) em relação a uma floresta ripária. O estudo foi desenvolvido no município de Cocalinho-MT. Os ensaios de capacidade de infiltração e resistência do solo à penetração foram realizados em transectos lineares. A pastagem em ambiente ripário apresentou significativas reduções de capacidade de infiltração e aumento da resistência do solo à penetração. Isso potencializa a ocorrência de processos erosivos e favorece a degradação de ecossistemas terrestres e aquáticos.The eff ects of land-use change (LUC) on the soil physical and hydraulic properties still need to be properly understood within the Araguaia River basin. In many cases, LUC occurs in riparian areas, and the negative consequences of its impacts are relevant to both terrestrial and aquatic ecosystems. In this paper, we examined the eff ect of LUC in the pasture (previously riparian area) and to riparian area. In doing so, we selected a pasture and a forest located in a riparian area. We developed the study in the municipality of Cocalinho-MT. We performed the infi ltration capacity and soil penetration resistance assays in linear transects. The pasture had lower infi ltration capacity and higher penetration resistance, attributed to trampling. Such changes can trigger erosion, which has the potential to degrade both terrestrial and aquatic ecosystems
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