1,542 research outputs found
A Novel Gonadotropin-Releasing Hormone 1 (Gnrh1) Enhancer-Derived Noncoding RNA Regulates Gnrh1 Gene Expression in GnRH Neuronal Cell Models.
Gonadotropin-releasing hormone (GnRH), a neuropeptide released from a small population of neurons in the hypothalamus, is the central mediator of the hypothalamic-pituitary-gonadal axis, and is required for normal reproductive development and function. Evolutionarily conserved regulatory elements in the mouse, rat, and human Gnrh1 gene include three enhancers and the proximal promoter, which confer Gnrh1 gene expression specifically in GnRH neurons. In immortalized mouse hypothalamic GnRH (GT1-7) neurons, which show pulsatile GnRH release in culture, RNA sequencing and RT-qPCR revealed that expression of a novel long noncoding RNA at Gnrh1 enhancer 1 correlates with high levels of GnRH mRNA expression. In GT1-7 neurons, which contain a transgene carrying 3 kb of the rat Gnrh1 regulatory region, both the mouse and rat Gnrh1 enhancer-derived noncoding RNAs (GnRH-E1 RNAs) are expressed. We investigated the characteristics and function of the endogenous mouse GnRH-E1 RNA. Strand-specific RT-PCR analysis of GnRH-E1 RNA in GT1-7 cells revealed GnRH-E1 RNAs that are transcribed in the sense and antisense directions from distinct 5' start sites, are 3' polyadenylated, and are over 2 kb in length. These RNAs are localized in the nucleus and have a half-life of over 8 hours. In GT1-7 neurons, siRNA knockdown of mouse GnRH-E1 RNA resulted in a significant decrease in the expression of the Gnrh1 primary transcript and Gnrh1 mRNA. Over-expression of either the sense or antisense mouse GnRH-E1 RNA in immature, migratory GnRH (GN11) neurons, which do not express either GnRH-E1 RNA or GnRH mRNA, induced the transcriptional activity of co-transfected rat Gnrh1 gene regulatory elements, where the induction requires the presence of the rat Gnrh1 promoter. Together, these data indicate that GnRH-E1 RNA is an inducer of Gnrh1 gene expression. GnRH-E1 RNA may play an important role in the development and maturation of GnRH neurons
The early history of protostellar disks, outflows, and binary stars
In star formation, magnetic fields act as a cosmic angular momentum extractor
that increases mass accretion rates onto protostars and in the process, creates
spectacular outflows. However, recently it has been argued that this magnetic
brake is so strong that early protostellar disks -- the cradles of planet
formation -- cannot form. Our three-dimensional numerical simulations of the
early stages of collapse (\lesssim 10^5 yr) of overdense star--forming clouds
form early outflows and have magnetically regulated and rotationally dominated
disks (inside 10 AU) with high accretion rates, despite the slip of the field
through the mostly neutral gas. We find that in three dimensions, magnetic
fields suppress gravitationally driven instabilities which would otherwise
prevent young, well ordered disks from forming. Our simulations have surprising
consequences for the early formation of disks, their density and temperature
structure, the mechanism and structure of early outflows, the flash heating of
dust grains through ambipolar diffusion, and the origin of planets and binary
stars.Comment: 12 pages, 3 figures, accepted by ApJ Letters; corrected text to match
journal version; movies can be found at
http://www.physics.mcmaster.ca/~duffindf/movies.htm
Equatorial ground ice on Mars: Steady-state stability
Current Martian equatorial surface temperatures are too warm for water ice to exist at the surface for any appreciable length of time before subliming into the atmosphere. Subsurface temperatures are generally warmer still and, despite the presence of a diffusive barrier of porous regolith material, it has been shown by Smoluchowski, Clifford and Hillel, and Fanale et al. that buried ground ice will also sublime and be lost to the atmosphere in a relatively short time. We investigate the behavior of this subliming subsurface ice and show that it is possible for ice to maintain at a steady-state depth, where sublimation and diffusive loss to the atmosphere is balanced by resupply from beneath by diffusion and recondensation of either a deeper buried ice deposits or ground water. We examine the behavior of equatorial ground ice with a numercial time-marching molecular diffusion model. In our model we allow for diffusion of water vapor through a porous regolith, variations in diffusivity and porosity with ice content, and recondensation of sublimed water vapor. A regolith containing considerable amounts of ice can still be very porous, allowing water vapor to diffuse up from deeper within the ice layer where temperatures are warmer due to the geothermal gradient. This vapor can then recondense nearer to the surface where ice had previously sublimed and been lost to the atmosphere. As a result we find that ice deposits migrate to find a steady-state depth, which represents a balance between diffusive loss to the atmosphere through the overlying porous regolith and diffusive resupply through a porous icy regolith below. This depth depends primarily on the long-term mean surface temperature and the nature of the geothermal gradient, and is independent of the ice-free porosity and the regolith diffusivity. Only the rate of loss of ground ice depends on diffusive properties
Data calibration for the MASCARA and bRing instruments
Aims: MASCARA and bRing are photometric surveys designed to detect
variability caused by exoplanets in stars with . Such variability
signals are typically small and require an accurate calibration algorithm,
tailored to the survey, in order to be detected. This paper presents the
methods developed to calibrate the raw photometry of the MASCARA and bRing
stations and characterizes the performance of the methods and instruments.
Methods: For the primary calibration a modified version of the coarse
decorrelation algorithm is used, which corrects for the extinction due to the
earth's atmosphere, the camera transmission, and intrapixel variations.
Residual trends are removed from the light curves of individual stars using
empirical secondary calibration methods. In order to optimize these methods, as
well as characterize the performance of the instruments, transit signals were
injected in the data. Results: After optimal calibration an RMS scatter of 10
mmag at is achieved in the light curves. By injecting transit
signals with periods between one and five days in the MASCARA data obtained by
the La Palma station over the course of one year, we demonstrate that MASCARA
La Palma is able to recover 84.0, 60.5 and 20.7% of signals with depths of 2, 1
and 0.5% respectively, with a strong dependency on the observed declination,
recovering 65.4% of all transit signals at versus 35.8% at
. Using the full three years of data obtained by MASCARA La
Palma to date, similar recovery rates are extended to periods up to ten days.
We derive a preliminary occurrence rate for hot Jupiters around A-stars of , knowing that many hot Jupiters are still overlooked. In the era of
TESS, MASCARA and bRing will provide an interesting synergy for finding
long-period ( days) transiting gas-giant planets around the brightest
stars.Comment: 18 pages, 17 figures, accepted for publication in A&
bRing: An observatory dedicated to monitoring the Pictoris b Hill sphere transit
Aims. We describe the design and first light observations from the
Pictoris b Ring ("bRing") project. The primary goal is to detect photometric
variability from the young star Pictoris due to circumplanetary
material surrounding the directly imaged young extrasolar gas giant planet
\bpb. Methods. Over a nine month period centred on September 2017, the Hill
sphere of the planet will cross in front of the star, providing a unique
opportunity to directly probe the circumplanetary environment of a directly
imaged planet through photometric and spectroscopic variations. We have built
and installed the first of two bRing monitoring stations (one in South Africa
and the other in Australia) that will measure the flux of Pictoris,
with a photometric precision of over 5 minutes. Each station uses two
wide field cameras to cover the declination of the star at all elevations.
Detection of photometric fluctuations will trigger spectroscopic observations
with large aperture telescopes in order to determine the gas and dust
composition in a system at the end of the planet-forming era. Results. The
first three months of operation demonstrate that bRing can obtain better than
0.5\% photometry on Pictoris in five minutes and is sensitive to
nightly trends enabling the detection of any transiting material within the
Hill sphere of the exoplanet
Identification of a putative man-made object from an underwater crash site using CAD model superimposition
In order to identify an object in video, a comparison with an exemplar object is typically needed. In this paper, we discuss the methodology used to identify an object detected in underwater video that was recorded during an investigation into Amelia Earhart\u27s purported crash site. A computer aided design (CAD) model of the suspected aircraft component was created based on measurements made from orthogonally rectified images of a reference aircraft, and validated against historical photographs of the subject aircraft prior to the crash. The CAD model was then superimposed on the underwater video, and specific features on the object were geometrically compared between the CAD model and the video. This geometrical comparison was used to assess the goodness of fit between the purported object and the object identified in the underwater video
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