11 research outputs found

    Perfil de unidades produtoras de leite de um laticínio da grande Florianópolis

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    TCC (graduação) - Universidade Federal de Santa Catarina. Centro de Ciências Agrárias. Curso de Zootecnia.O Brasil é o maior produtor de leite da América do sul e o quinto colocado em relação a produção mundial e tem a produção leiteira concentrada no Sudeste e no Sul do país. A produção de leite no estado de Santa Catarina, assim como no Brasil, vem crescendo consideravelmente nos últimos anos, sendo que grande parte da produção é composta principalmente de pequenas propriedades rurais com uma produção em escala de subsistência. Santa Catarina possui uma produção representativa e se concentra principalmente na região oeste do Estado. A região da Grande Florianópolis também se caracteriza como uma região produtora, porém com uma produção menos representativa. Desta forma, este estudo objetivou avaliar as condições da produção de leite em propriedades leiteiras da Região da Grande Florianópolis. Para isso foi elaborado um questionário com aproximadamente 30 perguntas relacionados à caracterização da propriedade rural, as características do rebanho, a quantidade da produção de leite, o manejo alimentar, a sanidade do rebanho e sobre as instalações da propriedade, e outros aspectos. O estudo foi realizado entre os meses de abril e maio de 2014 em 40 propriedades rurais na Grande Florianópolis. Os questionários foram preenchidos após a realização de uma entrevista com o produtor rural de forma individual. As propriedades rurais da Grande Florianópolis caracterizam-se pelo predomínio de pequenas propriedades, em termos de área, produção e número de animais, com prevalência de mão-de-obra familiar. Há uma predominância na utilização das raças leiteiras Jersey e Holandês As principais adversidades encontradas por esses produtores são a falta de assistência técnica e a relação desfavorável entre preço de insumos versus preço do leite, que são os principais motivos pela falta de investimento ou até mesmo a desistência da atividade

    Correção de hérnia perineal bilateral em canino com uso da deferentopexia: Relato de Caso

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    TCC(graduação) - Universidade Federal de Santa Catarina. Campus Curitibanos. Medicina Veterinária.A hérnia perineal é caracterizada pelo enfraquecimento da musculatura do diafragma pélvico, ou até mesmo pelo seu rompimento. Esse tipo de hérnia normalmente é relatada em cães machos, não castrados e idosos, podendo ocorrer também em gatos e cadelas jovens. O principal sinal clínico encontrado em animais com hérnia perineal é o aumento de volume nessa região além de tenesmo, constipação crônica, obstipação e disquesia. O tratamento terapêutico não é eficaz, sendo necessário, na maioria das vezes, realizar tratamento cirúrgico. Este estudo objetivou relatar o emprego da deferentopexia como técnica cirúrgica para a correção de uma hérnia perineal bilateral causada por uma hiperplasia prostática cística benigna em um cão, SRD, macho, não castrado de 20,7kg. A herniorrafia perineal tradicional associada a deferentopexia tem o intuito de diminuir a pressão sobre o diafragma pélvico e prevenir possíveis deslocamentos caudais dessas vísceras. O resultado não foi satisfatório, visto que houve recidiva após 15 dias. Uma nova herniorrafia perineal tradicional associada a uma tela de polipropileno foi realizada e após 20 dias o animal teve uma evolução satisfatória

    Computational biology helps understand how polyploid giant cancer cells drive tumor success

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    Precision and organization govern the cell cycle, ensuring normal proliferation. However, some cells may undergo abnormal cell divisions (neosis) or variations of mitotic cycles (endopolyploidy). Consequently, the formation of polyploid giant cancer cells (PGCCs), critical for tumor survival, resistance, and immortalization, can occur. Newly formed cells end up accessing numerous multicellular and unicellular programs that enable metastasis, drug resistance, tumor recurrence, and self-renewal or diverse clone formation. An integrative literature review was carried out, searching articles in several sites, including: PUBMED, NCBI-PMC, and Google Academic, published in English, indexed in referenced databases and without a publication time filter, but prioritizing articles from the last 3 years, to answer the following questions: (i) “What is the current knowledge about polyploidy in tumors?”; (ii) “What are the applications of computational studies for the understanding of cancer polyploidy?”; and (iii) “How do PGCCs contribute to tumorigenesis?

    Pervasive gaps in Amazonian ecological research

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    Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

    Pervasive gaps in Amazonian ecological research

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    Robust estimation of bacterial cell count from optical density

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    Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

    Pervasive gaps in Amazonian ecological research

    Get PDF
    Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

    Prognostic Factors and Markers in Non-Small Cell Lung Cancer: Recent Progress and Future Challenges

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    Lung cancer is a highly aggressive neoplasm and, despite the development of recent therapies, tumor progression and recurrence following the initial response remains unsolved. Several questions remain unanswered about non-small cell lung cancer (NSCLC): (1) Which patients will actually benefit from therapy? (2) What are the predictive factors of response to MAbs and TKIs? (3) What are the best combination strategies with conventional treatments or new antineoplastic drugs? To answer these questions, an integrative literature review was carried out, searching articles in PUBMED, NCBI-PMC, Google Academic, and others. Here, we will examine the molecular genetics of lung cancer, emphasizing NSCLC, and delineate the primary categories of inhibitors based on their molecular targets, alongside the main treatment alternatives depending on the type of acquired resistance. We highlighted new therapies based on epigenetic information and a single-cell approach as a potential source of new biomarkers. The current and future of NSCLC management hinges upon genotyping correct prognostic markers, as well as on the evolution of precision medicine, which guarantees a tailored drug combination with precise targeting
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