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Literatuuropvattingen en literaire praktijk, in samenhang met levensbeschouwelijke en maatschappelijke opvattingen gedurende het Interbellu

Target-Specificity in Scorpions; Comparing Lethality of Scorpion Venoms across Arthropods and Vertebrates

ABSTARCT: Scorpions use their venom in defensive situations as well as for subduing prey. Since some species of scorpion use their venom more in defensive situations than others, this may have led to selection for differences in effectiveness in defensive situations. Here, we compared the LD50 of the venom of 10 species of scorpions on five different species of target organisms; two insects and three vertebrates. We found little correlation between the target species in the efficacy of the different scorpion venoms. Only the two insects showed a positive correlation, indicating that they responded similarly to the panel of scorpion venoms. We discuss the lack of positive correlation between the vertebrate target species in the light of their evolution and development. When comparing the responses of the target systems to individual scorpion venoms pairwise, we found that closely related scorpion species tend to elicit a similar response pattern across the target species. This was further reflected in a significant phylogenetic signal across the scorpion phylogeny for the LD50 in mice and in zebrafish. We also provide the first mouse LD50 value for Grosphusgrandidieri

Base composition, selection, and phylogenetic significance of indels in the recombination activating gene-1 in vertebrates

<p>Abstract</p> <p>Background</p> <p>The Recombination Activating Proteins, RAG1 and RAG2, play a crucial role in the immune response in vertebrates. Among the nuclear markers currently used for phylogenetic purposes, <it>Rag1 </it>has especially enjoyed enormous popularity, since it successfully contributed to elucidating the relationships among and within a large variety of vertebrate lineages. We here report on a comparative investigation of the genetic variation, base composition, presence of indels, and selection in <it>Rag1 </it>in different vertebrate lineages (Actinopterygii, Amphibia, Aves, Chondrichthyes, Crocodylia, Lepidosauria, Mammalia, and Testudines) through the analysis of 582 sequences obtained from Genbank. We also analyze possible differences between distinct parts of the gene with different type of protein functions.</p> <p>Results</p> <p>In the vertebrate lineages studied, <it>Rag1 </it>is over 3 kb long. We observed a high level of heterogeneity in base composition at the 3^rdcodon position in some of the studied vertebrate lineages and in some specific taxa. This result is also paralleled by taxonomic differences in the GC content at the same codon position. Moreover, positive selection occurs at some sites in Aves, Lepidosauria and Testudines. Indels, which are often used as phylogenetic characters, are more informative across vertebrates in the 5' than in the 3'-end of the gene. When the entire gene is considered, the use of indels as phylogenetic character only recovers one major vertebrate clade, the Actinopterygii. However, in numerous cases insertions or deletions are specific to a monophyletic group.</p> <p>Conclusions</p> <p><it>Rag1 </it>is a phylogenetic marker of undoubted quality. Our study points to the need of carrying out a preliminary investigation on the base composition and the possible existence of sites under selection of this gene within the groups studied to avoid misleading resolution. The gene shows highly heterogeneous base composition, which affects some taxa in particular and contains sites under positive selection in some vertebrate lineages in the 5'-end. The first part of the gene (5'-end) is more variable than the second (3'-end), and less affected by a heterogeneous base composition. However, in some vertebrate lineages the 5'-end of the gene is not yet widely used for phylogenetic studies.</p

Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians

BACKGROUND: Identifying species of organisms by short sequences of DNA has been in the center of ongoing discussions under the terms DNA barcoding or DNA taxonomy. A C-terminal fragment of the mitochondrial gene for cytochrome oxidase subunit I (COI) has been proposed as universal marker for this purpose among animals. RESULTS: Herein we present experimental evidence that the mitochondrial 16S rRNA gene fulfills the requirements for a universal DNA barcoding marker in amphibians. In terms of universality of priming sites and identification of major vertebrate clades the studied 16S fragment is superior to COI. Amplification success was 100% for 16S in a subset of fresh and well-preserved samples of Madagascan frogs, while various combination of COI primers had lower success rates.COI priming sites showed high variability among amphibians both at the level of groups and closely related species, whereas 16S priming sites were highly conserved among vertebrates. Interspecific pairwise 16S divergences in a test group of Madagascan frogs were at a level suitable for assignment of larval stages to species (1–17%), with low degrees of pairwise haplotype divergence within populations (0–1%). CONCLUSION: We strongly advocate the use of 16S rRNA as standard DNA barcoding marker for vertebrates to complement COI, especially if samples a priori could belong to various phylogenetically distant taxa and false negatives would constitute a major problem

Modélisation des transformations pour l'évolution de modèles multidimensionnels

La modélisation et l'entreposage des données ont constitué, depuis plus d'une décennie, une problématique de recherche pour laquelle différentes approches ont été proposées. Ces approches se focalisent sur des aspects statiques de l'entrepôt de données. Or, l'évolution du système d'information qui alimente un entrepôt peut avoir un impact sur ce dernier et peut conduire, par conséquent, à l'évolution de son modèle multidimensionnel. Dans ce contexte évolutif, nous proposons une démarche dirigée par les modèles pour automatiser la propagation de l'évolution du modèle de la source de données relationnelle vers l'entrepôt. Cette démarche est fondée sur deux modèles d'évolution ainsi qu'un ensemble de règles de transformation formalisées en Query/View/Transformation. Nous développons un prototype logiciel nommé DWE (« Data Warehouse Evolution ») qui supporte cette démarche

History and structure of the closed pedigreed population of Icelandic Sheepdogs

<p>Abstract</p> <p>Background</p> <p>Dog breeds lose genetic diversity because of high selection pressure. Breeding policies aim to minimize kinship and therefore maintain genetic diversity. However, policies like mean kinship and optimal contributions, might be impractical. Cluster analysis of kinship can elucidate the population structure, since this method divides the population in clusters of related individuals. Kinship-based analyses have been carried out on the entire Icelandic Sheepdog population, a sheep-herding breed.</p> <p>Results</p> <p>Analyses showed that despite increasing population size and deliberately transferring dogs, considerable genetic diversity has been lost. When cluster analysis was based on kinships calculated seven generation backwards, as performed in previous studies, results differ markedly from those based on calculations going back to the founder-population, and thus invalidate recommendations based on previous research. When calculated back to the founder-population, kinship-based clustering reveals the distribution of genetic diversity, similarly to strategies using mean kinship.</p> <p>Conclusion</p> <p>Although the base population consisted of 36 Icelandic Sheepdog founders, the current diversity is equivalent to that of only 2.2 equally contributing founders with no loss of founder alleles in descendants. The maximum attainable diversity is 4.7, unlikely achievable in a non-supervised breeding population like the Icelandic Sheepdog. Cluster analysis of kinship coefficients can provide a supporting tool to assess the distribution of available genetic diversity for captive population management.</p

Phylogeography of Sardinian Cave Salamanders (Genus Hydromantes) Is Mainly Determined by Geomorphology

Detecting the factors that determine the interruption of gene flow between populations is key to understanding how speciation occurs. In this context, caves are an excellent system for studying processes of colonization, differentiation and speciation, since they represent discrete geographical units often with known geological histories. Here, we asked whether discontinuous calcareous areas and cave systems represent major barriers to gene flow within and among the five species of Sardinian cave salamanders (genus Hydromantes) and whether intraspecific genetic structure parallels geographic distance within and among caves. We generated mitochondrial cytochrome b gene sequences from 184 individuals representing 48 populations, and used a Bayesian phylogeographic approach to infer possible areas of cladogenesis for these species and reconstruct historical and current dispersal routes among distinct populations. Our results show deep genetic divergence within and among all Sardinian cave salamander species, which can mostly be attributed to the effects of mountains and discontinuities in major calcareous areas and cave systems acting as barriers to gene flow. While these salamander species can also occur outside caves, our results indicate that there is a very poor dispersal of these species between separate cave systems

Moleculaere phylogenie und biogeographie der ranoide froesche

Kenntnisse der evolutiven Verwandtschaftsverhältnisse bilden die Grundlage jeder vergleichenden Studie in der Biologie. Eine Erweiterung des Wissens über die Verwandtschaftsverhältnisse der Amphibien ist besonders erforderlich aufgrund der weltweit schrumpfenden Amphibienpopulationen. Durch einen generellen Mangel an Merkmalen und einem offensichtlich hohen Grad an Homoplasie bleibt die Systematik ranoider Frösche umstritten. In dieser Arbeit behandle ich die Systematik verschiedener Gruppen ranoider Frösche und verwandter Taxa unter Verwendung molekularer Merkmale, die aus der Analyse von DNA Sequenzen hervorgingen

An analysis of the live reptile and amphibian trade in the USA compared to the global trade in endangered species

The trade in wildlife is a globally important industry. Amphibians and reptiles are among the most commonly traded animals and this trade has raised concern because of its potential impact on natural populations, animal welfare and the spread of invasive species and emerging infectious diseases. Yet, evaluating the risks involved is difficult due to the lack of quantitative data on the trade. Here, we analyse data on the live reptile and amphibian trade in the USA and the worldwide trade in CITES-listed species over a ten year period. Our analyses show that the trade is dominated by only a few species, with ten species making up the majority of the trade. Moreover, our data show an increase of the contribution of captive bred specimens to the trade in the USA, but not worldwide. Our data do show the presence of several invasive species among those that are traded and bred most. The trade of potential invasive species is problematic and should be more strictly regulated as it may have a global impact on biodiversity and the spread of emerging infectious diseases