Supramolecular chemical shift reagents inducing conformational transitions: NMR analysis of carbohydrate homooligomer mixtures

Supramolecular chemical shift reagents improve signal resolution for NMR analysis of homooligosaccharides by inducing conformational transitions upon binding.</p

Vergleich von Energieaufwand, Futteraufnahme und Aktivität bei zwei Holstein Kuhtypen auf der Weide unter Bedingungen des biologischen Landbaus

In order to find cows well-adapted to organic production systems, the present study compared the energy expenditure, grass intake, grazing behavior and physical activity of two different Holstein strains in an organic full-time grazing system without concentrate supplementation. Twelve pairs of Swiss (HCH; 613 ± 34 kg BW) and New Zealand Holstein-Friesian (HNZ; 570 ± 54 kg BW) cows in mid lactation were investigated during a 7 d data collection period. The energy expenditure was analyzed using the 13C bicarbonate dilution technique in combination with an automatic blood sampling system during 6h per day. Although HCH and HNZ differed in bodyweight and seize, physical activity and energy expenditure only differed numerically between the two cow strains. As the grass intake and grazing behavior did not differ between strains, HCH could not use their full genetic potential for milk production. This short-time study indicates that cow strains adapt their behavior, bodyweight and production level to the environment of forage-based production systems. Consequently differences in energy expenditure become harder to detect

Digital Flourishing: Conceptualizing and Assessing Positive Perceptions of Mediated Social Interactions

Recent research started to apply concepts of well-being to the context of computer mediated communication (e.g., social media, instant messaging). While much research investigates negative perceptions of mediated social interactions (e.g., “problematic” or “addictive” social media use), a multi-dimensional measure that taps into users? positive perceptions is sorely lacking. The present research therefore develops the first comprehensive measure of digital flourishing, defined as positive perceptions of mediated social interactions. Building on a qualitative pre-study that aided the construction of the Digital Flourishing Scale (DFS), Study 1 (N = 474) employed exploratory factor analysis to reveal five subdimensions of digital flourishing. The preregistered Study 2 (N = 438) confirmed these five dimensions, yielding five reliable items per subscale and initial construct validity with three psychological needs from self-determination theory (SDT; competence, autonomy, relatedness) which were used as an underlying well-being framework for the development of the DFS. The preregistered Study 3 generated further construct validity by directly relating DFS to well-being. The scale is relevant for researchers and practitioners alike to better understand how users perceive their mediated interactions to impact mental health and well-being

Introduction: Affective Histories of Queer and Trans Activism

publishedVersionNon peer reviewe

Detection of postoperative granulation tissue with an ICG-enhanced integrated OI-/X-ray System

<p>Abstract</p> <p>Background</p> <p>The development of postoperative granulation tissue is one of the main postoperative risks after lumbar spine surgery. This granulation tissue may lead to persistent or new clinical symptoms or complicate a follow up surgery. A sensitive non-invasive imaging technique, that could diagnose this granulation tissue at the bedside, would help to develop appropriate treatments. Thus, the purpose of this study was to establish a fast and economic imaging tool for the diagnosis of granulation tissue after lumbar spine surgery, using a new integrated Optical Imaging (OI)/X-ray imaging system and the FDA-approved fluorescent contrast agent Indocyanine Green (ICG).</p> <p>Methods</p> <p>12 male Sprague Dawley rats underwent intervertebral disk surgery. Imaging of the operated lumbar spine was done with the integrated OI/X-ray system at 7 and 14 days after surgery. 6 rats served as non-operated controls. OI/X-ray scans of all rats were acquired before and after intravenous injection of the FDA-approved fluorescent dye Indocyanine Green (ICG) at a dose of 1 mg/kg or 10 mg/kg. The fluorescence signal of the paravertebral soft tissues was compared between different groups of rats using Wilcoxon-tests. Lumbar spines and paravertebral soft tissues were further processed with histopathology.</p> <p>Results</p> <p>In both dose groups, ICG provided a significant enhancement of soft tissue in the area of surgery, which corresponded with granulation tissue on histopathology. The peak and time interval of fluorescence enhancement was significantly higher using 10 mg/kg dose of ICG compared to the 1 mg/kg ICG dose. The levels of significance were p < 0.05. Fusion of OI data with X-rays allowed an accurate anatomical localization of the enhancing granulation tissue.</p> <p>Conclusion</p> <p>ICG-enhanced OI is a suitable technique to diagnose granulation tissue after lumbar spine surgery. This new imaging technique may be clinically applicable for postoperative treatment monitoring. It could be also used to evaluate the effect of anti-inflammatory drugs and may even allow evaluations at the bedside with new hand-held OI scanners.</p

Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues.

Lysine lactoylation is a recently described protein post-translational modification (PTM). However, the biochemical pathways responsible for this acylation remain unclear. Two metabolite-dependent mechanisms have been proposed: enzymatic histone lysine lactoylation derived from lactoyl-coenzyme A (lactoyl-CoA, also termed lactyl-CoA), and non-enzymatic lysine lactoylation resulting from acyl-transfer via lactoyl-glutathione. While the former has precedent in the form of enzyme-catalysed lysine acylation, the lactoyl-CoA metabolite has not been previously quantified in mammalian systems. Here, we use liquid chromatography-high-resolution mass spectrometry (LC-HRMS) together with a synthetic standard to detect and validate the presence of lactoyl-CoA in cell and tissue samples. Conducting a retrospective analysis of data from previously analysed samples revealed the presence of lactoyl-CoA in diverse cell and tissue contexts. In addition, we describe a biosynthetic route to generate 13C315N1-isotopically labelled lactoyl-CoA, providing a co-eluting internal standard for analysis of this metabolite. We estimate lactoyl-CoA concentrations of 1.14 × 10-8 pmol per cell in cell culture and 0.0172 pmol mg-1 tissue wet weight in mouse heart. These levels are similar to crotonyl-CoA, but between 20 and 350 times lower than predominant acyl-CoAs such as acetyl-, propionyl- and succinyl-CoA. Overall our studies provide the first quantitative measurements of lactoyl-CoA in metazoans, and provide a methodological foundation for the interrogation of this novel metabolite in biology and disease

NETWOODRESIST - An international network of open laboratories between Europe and Latin America to develop new tools on wood adaptative traits on drought stress and climatic change studies

International audienc

¿Qué calidad de madera produciremos en el futuro?: Los desafíos de integrar el valor adaptativo y tecnológico de la madera en un clima cambiantes

La madera cumple funciones de transporte y almacenamiento de agua, nutrientes y carbohidratos, fundamentales para la sobrevivencia de las especies leñosas frente a variaciones del ambiente. La densidad de la madera, una propiedad emergente de las características anatómicas (proporción de lúmenes y paredes celulares), se relaciona con la capacidad de conducción de agua en el xilema, siendo una variable clave en la arquitectura hidráulica de la planta. Sirve de soporte mecánico y es materia prima para la industria, con lo cual se constituye en una variable de gran valor tecnológico, cobrando relevancia para los procesos de producción, creación, diseño e implementación de productos para el agregado de valor en origen. Se suele afirmar que la densidad permite identificar la calidad de una madera en relación a su uso final: estimar su dureza, porosidad, comportamiento frente a esfuerzos mecánicos, cambios dimensionales, calidad de acabados y rendimiento de distintos procesos industriales, entre otros.Fil: Martinez Meier, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Sergent, Anne Sophie. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Dalla Salda, Guillermina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Caballe, Gonzalo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Rozenberg, Philippe. Institut National de la Recherche Agronomique; FranciaFil: Fernandez, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentin

¿Qué calidad de madera produciremos en el futuro?, un análisis sobre los desafíos de integrar valor adaptativo y tecnológico ante un clima cambiante

La madera cumple funciones de transporte y almacenamiento de agua, nutrientes y carbohidratos, fundamentales para la sobrevivencia de las especies leñosas frente a variaciones del ambiente. La densidad de la madera, una propiedad emergente de las características anatómicas (proporción de lúmenes y paredes celulares), se relaciona con la capacidad de conducción de agua en el xilema, siendo una variable clave en la arquitectura hidráulica de la planta. Sirve de soporte mecánico y es materia prima para la industria, con lo cual se constituye en una variable de gran valor tecnológico, cobrando relevancia para los procesos de producción, creación, diseño e implementación de productos para el agregado de valor en origen. Se suele afirmar que la densidad permite identificar la calidad de una madera en relación a su uso final: estimar su dureza, porosidad, comportamiento frente a esfuerzos mecánicos, cambios dimensionales, calidad de acabados y rendimiento de distintos procesos industriales, entre otros.Estación Experimental Agropecuaria BarilocheFil: Martinez Meier, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Recursos Forestales. Grupo de Ecología Forestal; ArgentinaFil: Sergent, Anne Sophie Marie. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Dalla Salda, Guillermina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Recursos Forestales. Grupo de Ecología Forestal; ArgentinaFil: Caballe, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experiemental Agropecuaria Bariloche. Área de Recursos Forestales. Grupo de Ecología Forestal; ArgentinaFil: Rozenberg, Philippe. Institut National de la Recherche Agronomique (INRA); FranciaFil: Fernandez, María Elena. INTA. Estación Experiemental Agropecuaria Balcarce. Agencia de Extensión Rural Tandil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin