An Electroanalytical Solution for the Determination of Pb2+ in Progressive Hair Dyes Using the Cork–Graphite Sensor

open7Lead is one of the most toxic metals for living organisms: once absorbed by soft tissues, it is capable of triggering various pathologies, subsequently bioaccumulating in the bones. In consideration of this, its detection and quantification in products for human consumption and use is of great interest, especially if the procedure can be carried out in an easy, reproducible and economical way. This work presents the results of the electroanalytical determination of lead in three different commercial products used as progressive hair dyes. Analyses were performed by cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV) using a composite cork–graphite sensor in 0.5M H2SO4 solution or 0.1M acetate buffer (pH 4.5), in the presence and absence of hair dye samples. The H2SO4 solution gave better results in terms of analyte sensitivity than the acetate buffer electrolyte. In both cases, well-defined signals for lead were obtained by DPSV analyses, enabling the calibration curve and figures of merit to be determined. The limits of detection (LOD) were found to be approximately 1.06 µM and 1.26 µM in H2SO4 and acetate buffer, respectively. The DPSV standard addition method was successfully applied to quantify the lead in hair dye samples, yielding values below 0.45% in Pb. All three analyzed samples were shown to comply with the limit set by the Brazilian Health Regulatory Agency, i.e., 0.6% lead in this type of product. The comparison of the electroanalytical results with those obtained by the reference method, based on the use of inductively coupled plasma optical emission spectrometry (ICP–OES), confirmed that the electroanalytical detection approach is potentially applicable as a strategy for quality control.openBarros, Thalita Medeiros; Medeiros de Araújo, Danyelle Medeiros de; Lemos de Melo, Alana Tamires Lemos de; Martínez-Huitle, Carlos Alberto; Vocciante, Marco; Ferro, Sergio; Vieira dos Santos, Elisama Vieira dosBarros, Thalita Medeiros; Medeiros de Araújo, Danyelle Medeiros de; Lemos de Melo, Alana Tamires Lemos de; Martínez-Huitle, Carlos Alberto; Vocciante, Marco; Ferro, Sergio; Vieira dos Santos, Elisama Vieira do

Detecção molecular de ovinos carreadores de Leptospira em ambiente tropical

The purpose of this study was to analyze the usefulness of PCR for the detection of leptospiral carriers in sheep under tropical field conditions. Two flocks, previously reported as seroreactive (A) and seronegative (B), were selected for this study. From those, the totality of animals of each flock, urine and vaginal fluid (VF)/semen were collected for bacteriological culture and PCR, as well as serum samples for serology. Serology confirmed the previous status of the two flocks. Culture was negative for all the samples. In PCR, animals of Flock A presented 26.7% (VF), 33.3% (semen) and 38.9% (urine) of positivity. Flock B presented 40.0% (VF), 33.3% (semen) and 5.6% (urine) of positivity by PCR. In conclusion, PCR was important to identify carriers of leptospires, including animals from a seronegative flock, what reinforces the advantages of the usage of this tool for the detection of carriers in sheep as part of control programs of leptospirosis under tropical field conditions.O objetivo do presente estudo foi analisar a aplicabilidade da PCR na detecção de ovinos carreadores de Leptospira em ambiente tropical. Brevemente, dois rebanhos ovinos, previamente reportados como sororeativo (A) e soronegativo (B) foram selecionados para este estudo. Da totalidade de animais de cada rebanho, amostras de urina e fluido vaginal (FV)/sêmen foram colhidas para cultura bacteriológica e PCR. Além disso, amostras de soro foram colhidas e utilizadas na sorologia (teste da soroaglutinação microscópica). Esta técnica confirmou o estado prévio dos dois rebanhos. Nenhuma amostra pura de leptospiras foi obtida no cultivo. Já na PCR, animais do Rebanho A apresentaram 26,7% (FV), 33,3% (sêmen) e 38,9% (urina) de amostras positivas. O Rebanho B apresentou 40,0% (FV), 33,3% (sêmen) e 5,6% (urina) de positividade pela PCR. Em conclusão, a PCR foi uma importante ferramenta na identificação de carreadores de leptospiras, incluindo animais do rebanho soronegativo, o que reforça as vantagens do uso desta técnica para a detecção de ovinos portadores como parte dos programas de controle da leptospirose em ambiente tropical.

Parallel implementation of Expectation-Maximisation algorithm for the training of Gaussian Mixture Models

Most machine learning algorithms need to handle large data sets. This feature often leads to limitations on processing time and memory. The Expectation-Maximization (EM) is one of such algorithms, which is used to train one of the most commonly used parametric statistical models, the Gaussian Mixture Models (GMM). All steps of the algorithm are potentially parallelizable once they iterate over the entire data set. In this study, we propose a parallel implementation of EM for training GMM using CUDA. Experiments are performed with a UCI dataset and results show a speedup of 7 if compared to the sequential version. We have also carried out modifications to the code in order to provide better access to global memory and shared memory usage. We have achieved up to 56.4% of achieved occupancy, regardless the number of Gaussians considered in the set of experiments

TERAPIA DE REPOSIÇÃO HORMONAL EM MULHERES NA MENOPAUSA: RISCOS E BENEFÍCIOS

O presente estudo busca trazer evidências sobre a terapia hormonal em mulheres na menopausa fisiológica, ou seja, que não foi causada por cirurgias ou iatrogenia, quanto aos seus riscos e benefícios. Deste modo, tratando-se de uma revisão integrativa, foi feita uma pesquisa na base de dados PubMed, em que foi utilizado os seguintes Descritores em Ciências da Saúde: hormone replacement therapy; menopause; risks and benefits. Sendo selecionados somente artigos publicados de 2022 a 2024, deste modo foram encontrados 24 artigos que após passarem pelos critérios de inclusão restaram-se 7 artigos revisados para a confecção deste trabalho. Menopausa é um período fisiológico na vida das mulheres caracterizado por cessação da atividade hormonal ovariana, junto com este período ocorre uma série de alterações no organismo feminino como os sintomas vasomotores, caracterizados por ondas de calor e distúrbios do sono, e a síndrome geniturinária, caracterizada por dispareunia, disúria e infecções do trato urinário. Tendo em vista a perda da qualidade de vida das mulheres neste período, faz-se necessária uma revisão da literatura sobre os principais riscos e benefícios do principal tratamento para estes sintomas que é a terapia de reposição hormonal. &nbsp

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection