Controlling Ectoparasites on Welsh Organic Sheep Farms

1. Organophosphate (OP) based dips offer a broad spectrum control against all major ectoparasites in the UK. However, there is continuing controversy over the safety to dip operators and the environment. The use of OPs is prohibited by organic standards, primarily because of concerns about mammalian toxicity. 2. Organic farmers are permitted to use synthetic pyrethroids (SPs)(dip products and pour-on products) and/or macrocyclic lactones (injectable products) to treat/control ectoparasites providing a derogation has been obtained from the certifying body. 3. SPs have been shown to be considerably more toxic to aquatic organisms than OPs. The environmental impact of SPs is not limited to levels in dip but also to residues in sheep fleeces. SPs may be removed from the wool by climatic conditions and then deposited in the local environment. 4. The Environment Agency have indicated that the majority of sheep dip pollution incidents involve SP dips. Concerns have been raised that as organic farmers can only use SP based dips they may be contributing to the higher levels of SP based pollution incidents. 5. There is little information as to current practices amongst organic sheep farmers to control/treat ectoparasites or on how these farmers dispose of the spent dip. In order to address this lack of information a survey was carried out by ADAS Pwllpeiran to investigate current practices amongst sheep farmers in Wales. For comparison purposes both conventional and organic farmers were included in the survey. 6. In total, 134 questionnaires were completed with 96 completed by conventional sheep farmers and the remaining 38 being completed by either in-conversion organic farmers or fully registered organic farmers. 7. Results showed that 58% of organic farmers surveyed treated their flocks for ectoparasites compared to 67% of conventional farmers. 8. Of the organic farmers surveyed, 52.6% listed flystrike as a parasite for which they treated their flocks. This was comparable to conventional farmers where 58.3% listed flystrike. Only 21.1% of organic farmers surveyed listed scab compared to 61.5% of conventional farmers. Less than 10% of both conventional and organic farmers surveyed treated for other ectoparasites. Those who did listed lice and ticks as the main ectoparasites. 9. Despite a large percentage of conventional farmers listing scab as an ectoparasite to be treated, only 11.5% of those surveyed treated for ‘scab only’ compared to 51.0% who treated for scab in combination with other ectoparasite control. A similar pattern was seen amongst organic farmers surveyed, where only 2.6% treated for ‘scab only’ whereas 18.4% treated for scab in combination with other ectoparasite control. 10. Of the organic farmers surveyed, 36.8% treated for ‘flystrike only’ compared to 7.3% of conventional farmers. 11. Despite other ectoparasites such as lice and ticks being listed by both conventional and organic farmers neither of these two parasites were treated singly but were always treated in combination with other ectoparasites. 12. Of conventional farmers surveyed who treated for ectoparasite control, 76.1% listed plunge dipping as the preferred dipping method compared to only 22.7% of the organic farmers surveyed who treated for ectoparasites. 13. The use of pour-on products was higher amongst organic farmers with 54.5% using pour-ons as a treatment method compared to 16.5% of conventional farmers. 14. Less than 5% of the organic farmers who treated for ectoparasites used a combination of treatments compared to 19% of conventional farmers who treated for ectoparasites. 15. When asked how often they treated for ectoparasites, 54% of organic farmers treated once a year compared to 42% of conventional farmers. 27% of organic farmers treated twice a year whereas 52% of conventional farmers treated twice a year. 14% of organic farmers treated three or more times a year compared to 7% of conventional farmers. 16. All organic farmers who carried out plunge dipping operations used a SP based product however of the conventional farmers who carried out plunge dipping, 39% used an SP based product. Overall, 16% of organic farmers surveyed used an SP based dip product compared to 24% of conventional farmers surveyed. Of all conventional farmers surveyed 38% used an OP based dip product. 17. Where farmers carried out plunge dipping, 53% of conventional farmers diluted spent dip before spreading to land compared to 83% of organic farmers who carried out plunge dipping operations. Of all the farmers surveyed who carried out plunge dipping only 1 treated dip with slaked lime before spreading. 18. Of all organic farmers surveyed, 5% used a mobile dipping contractor compared to 23% of conventional farmers surveyed. 19. Where contractors were used on organic holdings they were also responsible for the disposal of spent dip. The contractor was responsible for the disposal of dip on 74% of conventional holdings using contractors for dipping. 20. On 42% of the holdings using contractors, the spent dip was removed from the farm whereas on 32% of holdings the spent dip was spread on the farm land. 26% of farmers using contractors in this survey did not know how the contractor disposed of spent dip. 21. The percentage of farmers treating their flocks for ectoparasite infestations is similar for organic (58%) and conventional (69%) sheep farmers however there is a marked difference in the species of ectoparasites treated. 22. Of the conventional farmers who treated their flocks for ectoparasites, 86% listed scab as a major parasite compared to only 36% of organic farmers who also treated their flocks for ectoparasites. 23. Overall, only 3% of the organic farmers surveyed treated their sheep specifically for scab, compared to 11% of conventional farmers surveyed. 24. Of organic farmers surveyed, 37% treated their flocks specifically for blowfly strike compared to only 7% of conventional farmers surveyed. 25. Of the conventional farmers surveyed, 51% treated their flocks for more than one ectoparasite infestations compared to 18% of organic farmers surveyed. 26. From the evidence of several of the completed survey forms some farmers appeared to be using incorrect treatment methods and some were using multiple treatment methods to treat ectoparasite infestations. The use of incorrect treatments or multiple treatments is largely a reflection of the plethora of products available and suggests a lack of understanding as to which products are appropriate and licensed for the treatment of specific ectoparasites. 27. The survey found that fewer organic sheep farmers use SP dips than conventional sheep farmers do. As there are fewer organic sheep farmers overall, there is little evidence that organic sheep farmers contribute disproportionately to the level of SP based pollution incidents. 28. Summary of Recommendations: A. A further in-depth survey would be valuable to elucidate the extent of the scab problem in the national organic flock. B. Both organic and conventional farmers have difficulty in selecting the best/ most appropriate treatment for their flocks. An educational campaign would help to remedy this problem. C. Annual training courses for certifying bodies and advisors to keep up to date with developments in research and products are recommended. D. Given the potential for SP dips to cause major environmental damage if disposed of incorrectly it is a mandatory requirement that organic farmers are licensed by EA to dispose of spent dip. Inspectors should ensure cross compliance where organic farmers are using dip. E. Certifying bodies need to consider all the available evidence as to what chemicals should be permitted to treat scab and other ectoparasites and where appropriate make alterations to the standards. F. Inspection of dips facilities and EA licence should be part of the annual inspection procedures. G. Certification bodies should collate information on dipping practices and dip disposal. H. Where a pollution incident occurs on an organic holding this should be reported to the relevant certifying body. I. Development and evaluation of IPM programmes should be assessed as a management tool to reduce use of chemical treatments whilst promoting good animal husbandry and management. IPM programmes should be incorporated with animal health plans

Livestock breeds and Organic farming systems

Organic livestock farming has grown in Wales in recent years. It is focused on producing animals from a predominantly forage-based system, with an emphasis on maintaining animal health through improved welfare and a reduction in the use of routine, conventional veterinary treatments. Breeding and feeding are important factors of the health and welfare of farm animals in organic systems. The guidelines of EU regulation (EC) 1804/1999 address the issue of sourcing of (breeding) stock. Moreover, only activities such as the use of genetically modified organisms (GMOs) and embryo transfer are excluded, and few provisions are included that concern breeding. A number of breeds used in conventional farming could be considered as ’high maintenance’ animals requiring regular, prophylactic veterinary treatments and high-energy concentrated feeds to meet their potential. Such breeds may be unable to fulfil their potential performance under an organic system. A number of breeding strategies are outlined briefly, including traits that are important for organic production systems. Sire reference schemes have been introduced on organic farms in recent years. There is an increase in conservation grazing. Some breeds adapt well to these systems; especially local/native breeds since they utilise lower quality feed, are more resilient to climatic stress, and are more resistant to local parasites and diseases. Several actions are being, and have been, undertaken to maintain breed diversity. However, the need for a genetic pool of breeding stock from which to select is not reflected in the national strategies for livestock production. A Countryside Council for Wales (CCW) report (Yarwood and Evans, 2002) identified 18 breeds of sheep and 3 breeds of cattle associated with Wales. Several surveys have shown that, on organic farms, the Suffolk, Lleyn and the Texel are the main sheep breeds. The most popular beef breeds on organic farms are Welsh Black, Charolais crossbreed, Limousin, South Devon crossbreed, Aberdeen Angus and Hereford crossbreed. On organic farms, more native breeds seem to be used than on conventional farms. A few of these breeds are further discussed. Consumer demand is a significant driver of policy and standards, and value for money is still a major factor in determining consumer buying behaviour. The demand for lean meat and for homogeneity of both fresh and processed products, particularly in the major supermarkets, impacts on breeding policies. Research has shown that Welsh consumers prefer organic Welsh meat and milk. Economic viability of beef, sheep and milk production depends, in many cases, on subsidies. An increasing number of breed societies in Wales and the UK have become involved in promoting their breed as a brand. However, a recent Welsh survey showed that 13% of organically-produced lambs were still being sold as nonorganic due to finishing specifications (41%), a lack of organic market outlets (54%) and other reasons (4%), such as a lack of organic abattoirs (OCW, 2006). As market requirements are standardised and allow for little differentiation, some traditional and rare breeds face marketing difficulties. Furthermore, loss of small-scale abattoirs can reduce the ability for differentiation of breeds to enter niche markets. Large-scale abattoirs use standardised methods, which do not allow for differentiation. As mentioned in a report from DEFRA (2006), the national strategies for livestock production do not reflect the need for a genetic pool of breeding stock. Although breeding has to focus on what the market wants (mass or niche market), other factors also have to be taken into account. The choice of breeds/breeding used in the organic livestock sector needs to ensure the profitability of the farm, safeguard animal health and welfare, focus on conserving genetic diversity, and promote human health. The various breeds outlined in the report show various benefits. It is important to conserve, develop and utilise local breeds that are genetically adapted to their environment

Rhomboid family member 2 regulates cytoskeletal stress-associated Keratin 16.

Keratin 16 (K16) is a cytoskeletal scaffolding protein highly expressed at pressure-bearing sites of the mammalian footpad. It can be induced in hyperproliferative states such as wound healing, inflammation and cancer. Here we show that the inactive rhomboid protease RHBDF2 (iRHOM2) regulates thickening of the footpad epidermis through its interaction with K16. K16 expression is absent in the thinned footpads of irhom2-/- mice compared with irhom2+/+mice, due to reduced keratinocyte proliferation. Gain-of-function mutations in iRHOM2 underlie Tylosis with oesophageal cancer (TOC), characterized by palmoplantar thickening, upregulate K16 with robust downregulation of its type II keratin binding partner, K6. By orchestrating the remodelling and turnover of K16, and uncoupling it from K6, iRHOM2 regulates the epithelial response to physical stress. These findings contribute to our understanding of the molecular mechanisms underlying hyperproliferation of the palmoplantar epidermis in both physiological and disease states, and how this 'stress' keratin is regulated

Expanding dispersal studies at hydrothermal vents through species identification of cryptic larval forms

Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Marine Biology 157 (2010): 1049-1062, doi:10.1007/s00227-009-1386-8.The rapid identification of hydrothermal vent-endemic larvae to the species level is a key limitation to understanding the dynamic processes that control the abundance and distribution of fauna in such a patchy and ephemeral environment. Many larval forms collected near vents, even those in groups such as gastropods that often form a morphologically distinct larval shell, have not been identified to species. We present a staged approach that combines morphological and molecular identification to optimize the capability, efficiency, and economy of identifying vent gastropod larvae from the northern East Pacific Rise (NEPR). With this approach, 15 new larval forms can be identified to species. A total of 33 of the 41 gastropod species inhabiting the NEPR, and 26 of the 27 gastropod species known to occur specifically in the 9° 50’ N region, can be identified to species. Morphological identification efforts are improved by new protoconch descriptions for Gorgoleptis spiralis, Lepetodrilus pustulosus, Nodopelta subnoda, and Echinopelta fistulosa. Even with these new morphological descriptions, the majority of lepetodrilids and peltospirids require molecular identification. Restriction fragment length polymorphism digests are presented as an economical method for identification of five species of Lepetodrilus and six species of peltospirids. The remaining unidentifiable specimens can be assigned to species by comparison to an expanded database of 18S ribosomal DNA. The broad utility of the staged approach was exemplified by the revelation of species-level variation in daily planktonic samples and the identification and characterization of egg capsules belonging to a conid gastropod Gymnobela sp. A. The improved molecular and morphological capabilities nearly double the number of species amenable to field studies of dispersal and population connectivity.Funding was provided by as Woods Hole Oceanographic Institution Deep Ocean Exploration Institute grant to L.M and S. Beaulieu, National Science Foundation grants OCE-0424953, OCE-9712233, and OCE-9619605 to L.M, OCE-0327261 to T.S., and OCE-0002458 to K. Von Damm, and a National Defense Science and Engineering Graduate fellowship to D.A

Searching for Exoplanets Using a Microresonator Astrocomb

Detection of weak radial velocity shifts of host stars induced by orbiting planets is an important technique for discovering and characterizing planets beyond our solar system. Optical frequency combs enable calibration of stellar radial velocity shifts at levels required for detection of Earth analogs. A new chip-based device, the Kerr soliton microcomb, has properties ideal for ubiquitous application outside the lab and even in future space-borne instruments. Moreover, microcomb spectra are ideally suited for astronomical spectrograph calibration and eliminate filtering steps required by conventional mode-locked-laser frequency combs. Here, for the calibration of astronomical spectrographs, we demonstrate an atomic/molecular line-referenced, near-infrared soliton microcomb. Efforts to search for the known exoplanet HD 187123b were conducted at the Keck-II telescope as a first in-the-field demonstration of microcombs

Surviving Sepsis Campaign: International Guidelines for Management of Sepsis and Septic Shock: 2016.

OBJECTIVE: To provide an update to "Surviving Sepsis Campaign Guidelines for Management of Sepsis and Septic Shock: 2012." DESIGN: A consensus committee of 55 international experts representing 25 international organizations was convened. Nominal groups were assembled at key international meetings (for those committee members attending the conference). A formal conflict-of-interest (COI) policy was developed at the onset of the process and enforced throughout. A stand-alone meeting was held for all panel members in December 2015. Teleconferences and electronic-based discussion among subgroups and among the entire committee served as an integral part of the development. METHODS: The panel consisted of five sections: hemodynamics, infection, adjunctive therapies, metabolic, and ventilation. Population, intervention, comparison, and outcomes (PICO) questions were reviewed and updated as needed, and evidence profiles were generated. Each subgroup generated a list of questions, searched for best available evidence, and then followed the principles of the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) system to assess the quality of evidence from high to very low, and to formulate recommendations as strong or weak, or best practice statement when applicable. RESULTS: The Surviving Sepsis Guideline panel provided 93 statements on early management and resuscitation of patients with sepsis or septic shock. Overall, 32 were strong recommendations, 39 were weak recommendations, and 18 were best-practice statements. No recommendation was provided for four questions. CONCLUSIONS: Substantial agreement exists among a large cohort of international experts regarding many strong recommendations for the best care of patients with sepsis. Although a significant number of aspects of care have relatively weak support, evidence-based recommendations regarding the acute management of sepsis and septic shock are the foundation of improved outcomes for these critically ill patients with high mortality

Neuropsychological performance in solvent-exposed vehicle collision repair workers in New Zealand?

To assess whether solvent use and workplace practices in the vehicle collision repair industry are associated with symptoms of neurotoxicity in spray painters and panel beaters (auto body repair workers). Neurobehavioural symptoms were assessed using a cross-sectional study design in 370 vehicle collision repair and 211 reference workers using the EUROQUEST questionnaire. Full-shift airborne solvent levels were measured in a subset (n=92) of collision repair workers. Solvent exposures were higher in spray painters than in panel beaters, but levels were below current international exposure standards. Collision repair workers were more likely to report symptoms of neurotoxicity than reference workers with ORs of 2.0, 2.4 and 6.4 (all p<0.05) for reporting ≥5, ≥10 and ≥15 symptoms respectively. This trend was generally strongest for panel beaters (ORs of 2.1, 3.3 and 8.2 for ≥5, ≥10 and ≥15 symptoms respectively). Associations with specific symptom domains showed increased risks for neurological (OR 4.2), psychosomatic (OR 3.2), mood (OR 2.1), memory (OR 2.9) and memory and concentration symptoms combined (OR 2.4; all p<0.05). Workers who had worked for 10-19 years or 20+ years in the collision repair industry reported consistently more symptoms than those who had only worked less than 10 years even after adjusting for age. However, those who worked more than 20 years generally reported fewer symptoms than those who worked 10-19 years, suggesting a possible healthy worker survivor bias. Despite low airborne solvent exposures, vehicle collision repair spray painters and panel beaters continue to be at risk of symptoms of neurotoxicity

Using haloperidol as an anti-emetic in palliative care: informing practice through evidence from cancer treatment and post-operative contexts

YesNausea and vomiting are common symptoms in palliative care. Haloperidol is often used as an antiemetic in this context, although direct evidence supporting this practice is limited. To evaluate the efficacy and clinical use of haloperidol as an antiemetic in nonpalliative care contexts to inform practice, the authors conducted a rapid review of (i) published evidence to supplement existing systematic reviews, and (ii) practical aspects affecting the use of haloperidol including formulations and doses that are commonly available internationally. In nausea and vomiting related to cancer treatment, haloperidol was superior to control in two small studies. In postoperative nausea and vomiting (PONV), two randomized controlledtrials found treatment with haloperidol comparable to ondansetron. In palliative care, an observational study found a complete response rate of 24% with haloperidol (one in four patients) which would be consistent with a number needed to treat (NNT) of 3 to 5 derived from PONV. There remains insufficient direct evidence to definitively support the use of haloperidol for the management of nausea and vomiting in palliative care. However, generalizing evidence from other clinical contexts may have some validity

A Survey on the Krein-von Neumann Extension, the corresponding Abstract Buckling Problem, and Weyl-Type Spectral Asymptotics for Perturbed Krein Laplacians in Nonsmooth Domains

In the first (and abstract) part of this survey we prove the unitary equivalence of the inverse of the Krein--von Neumann extension (on the orthogonal complement of its kernel) of a densely defined, closed, strictly positive operator, $S\geq \varepsilon I_{\mathcal{H}}$ for some $\varepsilon >0$ in a Hilbert space $\mathcal{H}$ to an abstract buckling problem operator. This establishes the Krein extension as a natural object in elasticity theory (in analogy to the Friedrichs extension, which found natural applications in quantum mechanics, elasticity, etc.). In the second, and principal part of this survey, we study spectral properties for $H_{K,\Omega}$, the Krein--von Neumann extension of the perturbed Laplacian $-\Delta+V$ (in short, the perturbed Krein Laplacian) defined on $C^\infty_0(\Omega)$, where $V$ is measurable, bounded and nonnegative, in a bounded open set $\Omega\subset\mathbb{R}^n$ belonging to a class of nonsmooth domains which contains all convex domains, along with all domains of class $C^{1,r}$, $r>1/2$.Comment: 68 pages. arXiv admin note: extreme text overlap with arXiv:0907.144

Comparing methods to estimate treatment effects on a continuous outcome in multicentre randomized controlled trials: A simulation study

<p>Abstract</p> <p>Background</p> <p>Multicentre randomized controlled trials (RCTs) routinely use randomization and analysis stratified by centre to control for differences between centres and to improve precision. No consensus has been reached on how to best analyze correlated continuous outcomes in such settings. Our objective was to investigate the properties of commonly used statistical models at various levels of clustering in the context of multicentre RCTs.</p> <p>Methods</p> <p>Assuming no treatment by centre interaction, we compared six methods (ignoring centre effects, including centres as fixed effects, including centres as random effects, generalized estimating equation (GEE), and fixed- and random-effects centre-level analysis) to analyze continuous outcomes in multicentre RCTs using simulations over a wide spectrum of intraclass correlation (ICC) values, and varying numbers of centres and centre size. The performance of models was evaluated in terms of bias, precision, mean squared error of the point estimator of treatment effect, empirical coverage of the 95% confidence interval, and statistical power of the procedure.</p> <p>Results</p> <p>While all methods yielded unbiased estimates of treatment effect, ignoring centres led to inflation of standard error and loss of statistical power when within centre correlation was present. Mixed-effects model was most efficient and attained nominal coverage of 95% and 90% power in almost all scenarios. Fixed-effects model was less precise when the number of centres was large and treatment allocation was subject to chance imbalance within centre. GEE approach underestimated standard error of the treatment effect when the number of centres was small. The two centre-level models led to more variable point estimates and relatively low interval coverage or statistical power depending on whether or not heterogeneity of treatment contrasts was considered in the analysis.</p> <p>Conclusions</p> <p>All six models produced unbiased estimates of treatment effect in the context of multicentre trials. Adjusting for centre as a random intercept led to the most efficient treatment effect estimation across all simulations under the normality assumption, when there was no treatment by centre interaction.</p