The use of the LANDSAT data collection system and imagery in reservoir management and operation

The author has identified the following significant results. An increase in the data collection system's (DCS) ability to function in the flood control mission with no additional manpower was demonstrated during the storms which struck New England during April and May of 1975 and August 1976. It was found that for this watershed, creditable flood hydrographs could be generated from DCS data. It was concluded that an ideal DCS for reservoir regulation would draw features from LANDSAT and GOES. MSS grayscale computer printout and a USGS topographic map were compared, yielding an optimum computer classification map of the wetland areas of the Merrimack River estuary. A classification accuracy of 75% was obtained for the wetlands unit, taking into account the misclassified and the unclassified pixels. The MSS band 7 grayscale printouts of the Franklin Falls reservoir showed good agreement to USGS topographic maps in total area of water depicted at the low water reservoir stage and at the maximum inundation level. Preliminary analysis of the LANDSAT digital data using the GISS computer algorithms showed that the radiance of snow cover/vegetation varied from approximately 20 mW/sq cm sr in nonvegetated areas to less than 4 mW/sq cm sr for densely covered forested area

Chromosome Axis Defects Induce a Checkpoint-Mediated Delay and Interchromosomal Effect on Crossing Over during Drosophila Meiosis

Crossovers mediate the accurate segregation of homologous chromosomes during meiosis. The widely conserved pch2 gene of Drosophila melanogaster is required for a pachytene checkpoint that delays prophase progression when genes necessary for DSB repair and crossover formation are defective. However, the underlying process that the pachytene checkpoint is monitoring remains unclear. Here we have investigated the relationship between chromosome structure and the pachytene checkpoint and show that disruptions in chromosome axis formation, caused by mutations in axis components or chromosome rearrangements, trigger a pch2-dependent delay. Accordingly, the global increase in crossovers caused by chromosome rearrangements, known as the “interchromosomal effect of crossing over,” is also dependent on pch2. Checkpoint-mediated effects require the histone deacetylase Sir2, revealing a conserved functional connection between PCH2 and Sir2 in monitoring meiotic events from Saccharomyces cerevisiae to a metazoan. These findings suggest a model in which the pachytene checkpoint monitors the structure of chromosome axes and may function to promote an optimal number of crossovers

Skylab imagery: Application to reservoir management in New England

The author has identified the following significant results. S190B imagery is superior to the LANDSAT imagery for land use mapping and is as useful for level 1 and 2 land use mapping as the RB-57/RC8 high altitude imagery. Detailed land use mapping at levels 3 and finer from satellite imagery requires better resolution. For evaluating factors that are required to determine volume runoff potentials in a watershed, the S190B imagery was found to be as useful as the RB-57/RC8 high altitude aircraft imagery

All Paired Up with No Place to Go: Pairing, Synapsis, and DSB Formation in a Balancer Heterozygote

The multiply inverted X chromosome balancer FM7 strongly suppresses, or eliminates, the occurrence of crossing over when heterozygous with a normal sequence homolog. We have utilized the LacI-GFP: lacO system to visualize the effects of FM7 on meiotic pairing, synapsis, and double-strand break formation in Drosophila oocytes. Surprisingly, the analysis of meiotic pairing and synapsis for three lacO reporter couplets in FM7/X heterozygotes revealed they are paired and synapsed during zygotene/pachytene in 70%–80% of oocytes. Moreover, the regions defined by these lacO couplets undergo double-strand break formation at normal frequency. Thus, even complex aberration heterozygotes usually allow high frequencies of meiotic pairing, synapsis, and double-strand break formation in Drosophila oocytes. However, the frequencies of failed pairing and synapsis were still 1.5- to 2-fold higher than were observed for corresponding regions in oocytes with two normal sequence X chromosomes, and this effect was greatest near a breakpoint. We propose that heterozygosity for breakpoints creates a local alteration in synaptonemal complex structure that is propagated across long regions of the bivalent in a fashion analogous to chiasma interference, which also acts to suppress crossing over

Hydrothermally Emplaced, Lower Mississippian, Tripolitic Chert and Its Possible Relationship to the Tri-State Lead-Zinc Mining District

Across the southern Ozark Region, northern Arkansas, southwestern Missouri, and northeastern Oklahoma, exposures of the Lower Mississippian Boone Formation and its equivalents exhibit well-developed tripolitic chert that has been mined, more or less continuously, for at least 80 years. The tripolitic chert is a replacement of an interval within the basal portion of the upper Boone Formation in Arkansas and Oklahoma, and equivalent to the Elsey Formation in Missouri. The movement of silica-rich, hydrothermal fluids appears to have been much like that of a confined aquifer. It followed the basal upper Boone Formation (Arkansas) = Elsey Formation (Missouri) and was bound below by an impermeable interval at the top of the lower Boone Formation (Arkansas) = Reeds Spring Formation (Missouri), and above by the base of the upper Boone Formation (Arkansas) = Burlington-Keokuk (Missouri). The first hydrothermal event incompletely silicified the basal upper Boone = Elsey Formation. After leaching of the remnant carbonate, thus forming the tripolitic chert, a second hydrothermal event deposited terminated and doubly terminated quartz crystals, and druse in the tripolitic chert voids. This hydrothermal event may have pro-duced the Mississippi Valley-Type (MVT) lead-zinc deposits in northeast Oklahoma and southwestern Missouri. The famous deposits at Picher, Oklahoma, and Joplin, Missouri, appear to be positioned in the apparent path of the hydrothermal fluid migration. While timing of these hydrothermal events is unclear, they may reflect lateral secretion produced by the Ouachita Orogeny in the Late Pennsylvanian

Temporal Analysis of Meiotic DNA Double-Strand Break Formation and Repair in Drosophila Females

Using an antibody against the phosphorylated form of His2Av (γ-His2Av), we have described the time course for the series of events leading from the formation of a double-strand break (DSB) to a crossover in Drosophila female meiotic prophase. MEI-P22 is required for DSB formation and localizes to chromosomes prior to γ-His2Av foci. Drosophila females, however, are among the group of organisms where synaptonemal complex (SC) formation is not dependent on DSBs. In the absence of two SC proteins, C(3)G and C(2)M, the number of DSBs in oocytes is significantly reduced. This is consistent with the appearance of SC protein staining prior to γ-His2Av foci. However, SC formation is incomplete or absent in the neighboring nurse cells, and γ-His2Av foci appear with the same kinetics as in oocytes and do not depend on SC proteins. Thus, competence for DSB formation in nurse cells occurs with a specific timing that is independent of the SC, whereas in the oocytes, some SC proteins may have a regulatory role to counteract the effects of a negative regulator of DSB formation. The SC is not sufficient for DSB formation, however, since DSBs were absent from the heterochromatin even though SC formation occurs in these regions. All γ-His2Av foci disappear before the end of prophase, presumably as repair is completed and crossovers are formed. However, oocytes in early prophase exhibit a slower response to X-ray–induced DSBs compared to those in the late pachytene stage. Assuming all DSBs appear as γ-His2Av foci, there is at least a 3:1 ratio of noncrossover to crossover products. From a comparison of the frequency of γ-His2Av foci and crossovers, it appears that Drosophila females have only a weak mechanism to ensure a crossover in the presence of a low number of DSBs

Preserving and sharing born-digital and hybrid objects from and across the National Collection

This report is one of a set of outputs from the Arts and Humanities Research Council funded project ‘Preserving and sharing born-digital and hybrid objects from and across the National Collection’. It has been designed to provide an extensive account of the project research activities and findings, to be useful to museum, heritage, and preservation professionals, as well as to scholars interested in born-digital materials. The aims of the project were to instigate a conversation and build confidence across the museum sector to support the collecting of born-digital objects, and to lay the foundations for future research in the field. The research gathers the expertise of professionals from different backgrounds, and has an international ambition; however, institutions addressing this type of collections tend to be concentrated in a few countries across Europe, Australia and North America. The research’s methodology includes: desk-based research, the focused investigation of four case studies, interviews and workshops. The analysis of the data collected has supported the articulation of a set of themes and key ideas that provide the grounding for the expression of policy, research and practice-related recommendations. The report understands the challenges of collecting born-digital objects as going beyond the mere technical realm of obsolescence and broken dependencies, to address issues of legality, visibility and accountability. It discusses the multi-layered and complex authorship of many born-digital objects associated with communities or corporate ownership, and expands on the potential of collaborative approaches to collection stewardship