Standardizing design performance comparison in microfluidic manufacturing

Microfluidic devices published in literature today lack sufficient information for automating the physical design process. Moreover, the constantly changing landscape of manufacturing and technological requirements poses a large problem in the physical design automation space. In this talk, we discuss some of the methodologies and standards formulated by CIDAR at BU and CARES at UC Riverside that allow not only allow the researchers in the physical design automation space to share and compare their results but also provide means for capturing the Specify, Design and Build lifecycle in microfluidic design

Smoking behaviour predicts tobacco control attitudes in a high smoking prevalence hospital: A cross-sectional study in a Portuguese teaching hospital prior to the national smoking ban

<p>Abstract</p> <p>Background</p> <p>Several studies have investigated attitudes to and compliance with smoking bans, but few have been conducted in healthcare settings and none in such a setting in Portugal. Portugal is of particular interest because the current ban is not in line with World Health Organization recommendations for a "100% smoke-free" policy. In November 2007, a Portuguese teaching-hospital surveyed smoking behaviour and tobacco control (TC) attitudes before the national ban came into force in January 2008.</p> <p>Methods</p> <p>Questionnaire-based cross-sectional study, including all eligible staff. Sample: 52.9% of the 1, 112 staff; mean age 38.3 ± 9.9 years; 65.9% females. Smoking behaviour and TC attitudes and beliefs were the main outcomes. Bivariable analyses were conducted using chi-squared and MacNemar tests to compare categorical variables and Mann-Whitney tests to compare medians. Multilogistic regression (MLR) was performed to identify factors associated with smoking status and TC attitudes.</p> <p>Results</p> <p>Smoking prevalence was 40.5% (95% CI: 33.6-47.4) in males, 23.5% (95% CI: 19.2-27.8) in females (p < 0.001); 43.2% in auxiliaries, 26.1% in nurses, 18.9% among physicians, and 34.7% among other non-health professionals (p = 0.024). The findings showed a very high level of agreement with smoking bans, even among smokers, despite the fact that 70.3% of the smokers smoked on the premises and 76% of staff reported being frequently exposed to second-hand smoke (SHS). In addition 42.8% reported that SHS was unpleasant and 28.3% admitted complaining. MLR showed that smoking behaviour was the most important predictor of TC attitudes.</p> <p>Conclusions</p> <p>Smoking prevalence was high, especially among the lower socio-economic groups. The findings showed a very high level of support for smoking bans, despite the pro-smoking environment. Most staff reported passive behaviour, despite high SHS exposure. This and the high smoking prevalence may contribute to low compliance with the ban and low participation on smoking cessation activities. Smoking behaviour had greater influence in TC attitudes than health professionals' education. Our study is the first in Portugal to identify potential predictors of non-compliance with the partial smoking ban, further emphasising the need for a 100% smoke-free policy, effective enforcement and public health education to ensure compliance and promote social norm change.</p

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Spatial Dynamics of Sea Turtle Abundance and Shrimping Intensity in the U.S. Gulf of Mexico

In order to examine the scientific feasibility of area closures for sea turtle protection, we determined the spatial dynamics of sea turtles for the U.S. Gulf of Mexico by analyzing National Marine Fisheries Service (NMFS) aerial survey data in September, October, and November of 1992, 1993, and 1994. Turtle sightings were grouped into depth zones and NMFS fishery statistical zones, and strip transect methods were used to estimate the relative abundance of sea turtles in each subzone. Average shrimping intensity was calculated for each subzone for all months of 1992, 1993, and 1994, as well as for the months and locations of the aerial survey. The spatial overlap of sea turtle abundance and shrimping intensity suggested regions where interactions are likely to occur. Sea turtles were observed at much higher rates along the coast of Florida than in the Western Gulf; the highest density of sea turtles was observed in the Florida Keys region (0.525 turtles/km2). Shrimping intensity was highest in the Western Gulf along the coast of Texas and Louisiana, for both annual and fall estimates. Among alternative management scenarios, area closures in conjunction with continued Turtle Excluder Device (TED) requirements would probably best prevent sea turtles from future extinction. By implementing shrimping closures off of South Padre Island, Texas, a potential second nesting population of Kemp's ridleys (Lepidochelys kempi) could be protected. Closing waters where shrimping intensity is low and sea turtle abundance is high (e.g., South Florida waters) would protect sea turtles without economically impacting a large number of shrimpers

Forming a flossing habit: an exploratory study of the psychological determinants of habit formation.

OBJECTIVES: Habit formation has been proposed as a means to promote maintenance of healthy behaviours, but there have been few investigations into how habits are formed. This exploratory study sought to model determinants of the formation of a dental flossing habit, including placement of the behaviour within the routine (before vs. after tooth-brushing), past behaviour, prospective memory ability, and motivational factors. DESIGN AND METHOD: All participants (N = 50) received a motivational intervention designed to initiate behaviour change and habit formation. Half of the participants were instructed to floss before brushing, and half after. Participants subsequently self-reported flossing behaviour daily and, 4 weeks later, flossing automaticity. Automaticity and flossing frequency were also measured at 8-month follow-up. RESULTS: Participants with stronger prospective memory ability, higher levels of past behaviour, and a more positive attitude flossed more frequently during the study. Stronger automaticity was predicted by positive attitudes, and increased behaviour frequency during and prior to the study. Those who flossed after brushing (rather than before) tended to form stronger flossing habits and, at 8-month follow-up, had stronger habits and flossed more frequently. CONCLUSIONS: Habit forming interventions might usefully consider features of everyday routines and how behaviour may be reinforced. Suggestions for further research using more methodologically rigorous designs are offered. STATEMENT OF CONTRIBUTION: What is already known on this subject? The formation of habit - that is, a learnt automatic response to contextual cues - requires initiation of a behaviour and repetition in a constant context. A recent formation study showed variation in habit strength despite equal repetitions, indicating that factors other than repetition may be important in habit development. From studies of routine behaviour, the boundaries between sub-routines are characterized by different processes than the middle of sub-routines, suggesting that placement of behaviour within existing routines may affect the likelihood of habit formation. What does this study add? Greater prospective memory ability predicted more frequent dental flossing. Initiating flossing after rather than before tooth-brushing promoted stronger habits. Positive attitudes impacted directly on habit formation, independent of behaviour repetition

EVALUATION OF ULTRA-HIGH PERFORMANCE CONCRETE FOR USE IN BRIDGE CONNECTIONS AND REPAIR (FHWA-OK-21-03)

The project described in this report evaluated available proprietary ultra-high performance concrete (UHPC) materials and UHPC mix designs made with local materials for applicability to bridge joint installation and repair in Oklahoma and developed recommendations for continued usage of UHPC in bridge construction in Oklahoma. Phase 1 of the project developed a promising UHPC mix design, J3, using local materials. Two specific applications of UHPC were considered: deck slab joints and girder continuity connections. Initial investigation of deck slab joint details was conducted using small-scale flexural specimens to evaluate bond strength between UHPC and base concrete. Laboratory-scale full-depth joints were cast and tested using both the proprietary UHPC material and the OU developed J3 mix design. Laboratory scale UHPC connections for live load continuity between precast girders were also designed and tested to failure. Two different connection details were used, one representing new construction and one representing retrofit of an existing structure. A field test involving retrofit of an existing expansion joint with UHPC joint headers was conducted on a bridge identified in conjunction with ODOT and was monitored for almost three years. Phase 2 involved slab testing for a partial depth slab joint detail, examination of reinforcement bond, and durability testing of both proprietary and non-proprietary UHPC. UHPC bond to concrete substrate was also examined for different surface preparations and base concrete saturation levels. The findings of the research indicate that UHPC provides improved performance relative to conventional materials for the applications tested and the J3 mix design exhibits similar performance to proprietary UHPC.Final Report October 2016 - October 2020N