Halogen occultation experiment intergrated test plan

The test program plan is presented for the Halogen Occultation Experiment (HALOE) instrument, which is being developed in-house at the Langley Research Center for the Upper Atmosphere Research Satellite (UARS). This comprehensive test program was developed to demonstrate that the HALOE instrument meets its performance requirements and maintains integrity through UARS flight environments. Each component, subsystem, and system level test is described in sufficient detail to allow development of the necessary test setups and test procedures. Additionally, the management system for implementing this test program is given. The HALOE instrument is a gas correlation radiometer that measures vertical distribution of eight upper atmospheric constituents: O3, HC1, HF, NO, CH4, H2O, NO2, and CO2

Archeological Testing and Data Recovery at the Flatrock Road Site, 41KM69, Kimble County, Texas

The Center for Archaeological Research (CAR) of The University of Texas at San Antonio (UTSA) conducted archeological significance testing and data recovery excavations at 41KM69, the Flatrock Road Site, at the request of the Texas Department of Transportation, Environmental Affairs Division (TxDOT-ENV). The significance testing was begun in 2004 under Texas Antiquities Permit No. 3350 to determine National Register of Historic Places eligibility status of the site and continued to the data recovery phase in 2005 under Texas Antiquities Permit No. 3584 with Raymond Mauldin serving as Principal Investigator on both permits. Work was begun in anticipation of alterations to Flatrock Road/FM 2169 that intersects the site because TxDOT and the Texas Historical Commission concurred with CAR’s assessment that the site was eligible for listing on the NRHP and because TxDOT could not avoid impacts to intact deposits. During testing 120 auger tests, six backhoe trenches, eight 50-x-50-cm units, and five 1-x-1-m units were excavated across the western portion of the site within the planned TxDOT right-of-way (ROW). This effort confirmed intact Late Archaic and Late Prehistoric Austin and Toyah interval components as well as a disturbed twentieth century component. The prehistoric deposits included four burned rock features including a large burned rock midden, 3,000 chipped stone artifacts, 10 temporally diagnostic projectile points, unifacial tools typical of Toyah end-scrapers, and native ceramics, also commonly found on Toyah occupations. A small quantity of bone was collected including tibia fragments from one bison. The historic component was confined to the upper level of the site from the surface to approximately 20 cmbs. The Kimble Courts resort camp once stood in the area. Data recovery excavations targeted the prehistoric components after the historic levels were removed by backhoe. Four large blocks were excavated in areas of high artifact density. Approximately 130 m3 and 40,000 artifacts were recovered from these blocks including 350 stone tools and 114 earthenware sherds. Projectile point types found were Castroville, Pedernales, Montell, Ellis, Frio, Ensor, Fairland, Edwards, and Perdiz. Seventy-three prehistoric thermal rock features and soil stains were also recorded. We also collected bone, shell, feature burned rock, and soil samples. Following hand excavations, the project area was monitored during Gradall stripping of the remaining deposits. Fifty-seven auger tests were also excavated after a shift in the project ROW. Artifact density was sparse in the shifted ROW, and most artifacts came from disturbed upper level deposits. CAR developed a research design in consultation with TxDOT after all excavations were completed. This research focused on the interpretation of the deposits discovered during significance testing and data recovery from 41KM69 and a number of comparative sites around Texas. The theoretical framework draws from principles of cultural and evolutionary ecology to examine shifts in subsistence, technology, and mobility in hunter-gatherers from the Late Archaic and Late Prehistoric periods of South and Central Texas. All artifacts and samples collected during this project, along with project related documentation are to be permanently curated at CAR according to THC guidelines

Archeological Testing and Data Recovery at the Flatrock Road Site, 41KM69, Kimble County, Texas

The Center for Archaeological Research (CAR) of The University of Texas at San Antonio (UTSA) conducted archeological significance testing and data recovery excavations at 41KM69, the Flatrock Road Site, at the request of the Texas Department of Transportation, Environmental Affairs Division (TxDOT-ENV). The significance testing was begun in 2004 under Texas Antiquities Permit No. 3350 to determine National Register of Historic Places eligibility status of the site and continued to the data recovery phase in 2005 under Texas Antiquities Permit No. 3584 with Raymond Mauldin serving as Principal Investigator on both permits. Work was begun in anticipation of alterations to Flatrock Road/FM 2169 that intersects the site because TxDOT and the Texas Historical Commission concurred with CAR’s assessment that the site was eligible for listing on the NRHP and because TxDOT could not avoid impacts to intact deposits. During testing 120 auger tests, six backhoe trenches, eight 50-x-50-cm units, and five 1-x-1-m units were excavated across the western portion of the site within the planned TxDOT right-of-way (ROW). This effort confirmed intact Late Archaic and Late Prehistoric Austin and Toyah interval components as well as a disturbed twentieth century component. The prehistoric deposits included four burned rock features including a large burned rock midden, 3,000 chipped stone artifacts, 10 temporally diagnostic projectile points, unifacial tools typical of Toyah end-scrapers, and native ceramics, also commonly found on Toyah occupations. A small quantity of bone was collected including tibia fragments from one bison. The historic component was confined to the upper level of the site from the surface to approximately 20 cmbs. The Kimble Courts resort camp once stood in the area. Data recovery excavations targeted the prehistoric components after the historic levels were removed by backhoe. Four large blocks were excavated in areas of high artifact density. Approximately 130 m3 and 40,000 artifacts were recovered from these blocks including 350 stone tools and 114 earthenware sherds. Projectile point types found were Castroville, Pedernales, Montell, Ellis, Frio, Ensor, Fairland, Edwards, and Perdiz. Seventy-three prehistoric thermal rock features and soil stains were also recorded. We also collected bone, shell, feature burned rock, and soil samples. Following hand excavations, the project area was monitored during Gradall stripping of the remaining deposits. Fifty-seven auger tests were also excavated after a shift in the project ROW. Artifact density was sparse in the shifted ROW, and most artifacts came from disturbed upper level deposits. CAR developed a research design in consultation with TxDOT after all excavations were completed. This research focused on the interpretation of the deposits discovered during significance testing and data recovery from 41KM69 and a number of comparative sites around Texas. The theoretical framework draws from principles of cultural and evolutionary ecology to examine shifts in subsistence, technology, and mobility in hunter-gatherers from the Late Archaic and Late Prehistoric periods of South and Central Texas. All artifacts and samples collected during this project, along with project related documentation are to be permanently curated at CAR according to THC guidelines

KRT5 missense variant in a Cardigan Welsh Corgi with epidermolysis bullosa simplex.

Epidermolysis bullosa (EB) is a group of blistering disorders that includes several subtypes, classified according to their level of cleavage. Typical clinical signs are blisters and erosions resulting from minimal trauma. The disease has been described in many mammalian species and pathogenic variants in at least 18 different genes have been identified. In the present study, we investigated a Cardigan Welsh Corgi with congenital clinical signs consistent with epidermolysis bullosa. The puppy had blisters and erosions on the paw pads, and the oral mucosa. Histologic examination demonstrated the typical clefting between the dermis and epidermis and confirmed the clinical suspicion. We obtained whole genome sequencing data from the affected puppy and searched for variants in candidate genes known to cause EB. This revealed a heterozygous missense variant, KRT5:p.(E476K), affecting the highly conserved KLLEGE motif of keratin 5. The mutant allele in the affected puppy arose owing to a de novo mutation event as it was absent from both unaffected parents. Knowledge of the functional impact of KRT5 variants in other species together with the demonstration of the de novo mutation event establishes KRT5:p.(E476K) as causative variant for the observed EBS

Results of Archeological Significance Testing at 41TV410 and 41TV540 and Associated Geomorphological Investigations on a Segment of Onion Creek in Travis County, Texas

In late 2005, the Texas Department of Transportation (TxDOT) contracted the Center for Archaeological Research at the University of Texas at San Antonio (CAR-UTSA) to conduct significance testing at 41TV410 and 41TV540, two prehistoric archeological sites located in the floodplain of Onion Creek in southeast Austin in Travis County. The work was conducted in advance of a borrow pit excavation related to the construction on State Highway (SH) 130. The borrow pit represented a project specific location (PSL). PSLs are normally not part of the project per Federal Highway Administration policy. PSLs are the responsibility of the contractor in most cases. However, the testing reported here was mandated by language of the SH 130 comprehensive development agreement. Significance testing was performed late in 2005 and early in 2006. For 41TV540, Dr. Raymond Mauldin served as Principal Investigator and Jennifer Thompson served as the Project Archeologist. At 41TV410, Dr. Mauldin served as Project Archeologist and Dr. Steve Tomka served as Principal Investigator. The archeological work at these sites was not under the purview of either Section 106 or the Antiquities Code of Texas. The work was not conducted under a Texas Antiquities Permit. However, testing at both sites was conducted in accordance with the Secretary of the Interior’s Standards and Guidelines for Archeology and Historic Preservation and guidelines provided by the Texas Historical Commission (THC). CAR completed fieldwork in early January of 2006. At 41TV410, 11 features were uncovered with radiocarbon dates that fell in the Early/Middle Archaic and in the Late Archaic. Recovery of artifacts and ecofacts was extremely low. The 41TV540 work also produced a low density of chipped stone artifacts and tools, and exposed 17 features, many of which dated to the close of the Early Archaic. Preliminary observations on flotation returns suggested good quantities of burned material and faunal remains were present at 41TV540. CAR prepared post-field reports summarizing the work and provided recommendations for both sites. Because both locations appeared to contain intact deposits that could, in our view, contribute to an understanding of the prehistoric cultural development of the region, CAR recommended that the sites were eligible to the NRHP under criterion d. TxDOT agreed with CAR recommendations that 41TV540 was eligible for listing in the NRHP, though they did not request any additional work at the site. TxDOT concluded that site 41TV410 was not eligible for listing on the NRHP. At TxDOT’s instruction, CAR prepared a research design for the analysis of the testing data from 41TV540, as well as a summary of the 41TV410 work. That document (Tomka et al. 2007), which outlined a series of analyses including several new geomorphic studies and a project area geomorphic synthesis, served as a guideline for the present document. Since the archeological and geomorphological investigations reported here, the construction of SH 130 has been completed and deposits containing the sites discussed have been removed. All artifacts, notes, photos, and other material associated with the project are stored at CAR. At this time, decisions regarding the disposal of selected artifact classes, as well as the location for permanent curation of the remaining artifacts and associated records, have not been made. However, it is expected that all project related records and artifacts retained will be permanently curated at CAR

Investigations into free tropospheric new particle formation in the central Canadian arctic during the winter/spring transition as part of TOPSE

In this paper, we investigate the role of in situ new particle production in the central Canadian sub-Arctic and Arctic as part of the TOPSE experiment. Airborne measurements conducted primarily in the free troposphere were made from 50° to 90°W longitude and 60° to 85°N latitude during the period from February to May 2000. Data pertinent to this paper include 3–4 nm diameter (Dp) particles, ultrafine condensation nuclei (Dp \u3e 3 nm), fine particles (0.2 \u3c Dp \u3c 3 μm), and the possible nucleation precursor, sulfuric acid, and its precursor, sulfur dioxide. For data averaged over this period, most species showed little evidence for a latitudinal trend. Fine aerosol number concentrations, however, showed a slight increase with latitude. The evolution of various species concentrations over the period of the study show that fine particles also had a consistent temporal trend, increasing at all altitudes from February to May, whereas sulfur dioxide at the surface tended to peak in late March. Ultrafine condensation nuclei and 3–4 nm particles showed no temporal trends. Little evidence for in situ new particle production was observed during the study, except for one atypical event where SO2concentrations were 3.5 ppbv, 2 orders of magnitude higher than typical levels. This paper cannot address the question of whether the observed condensation nuclei were produced in situ by a low particle production rate or transported from lower latitudes

DEVELOPMENT OF A SYNTHETIC MATERIALS MIMIC FOR VULTURE OLFACTION RESEARCH

Since the early 1990\u27s, an increase in damage associated with the activities of black (Coragyps atratus) and turkey (Cathartes aura) vultures has been observed. These activities include extensive damage to real and personal property. Vultures display an affinity for objects made of synthetic materials such as vinyl or plastic, and frequently damage items such as seat cushions, roof shingles, and caulking sealant. To explore whether this behavior in vultures is olfactorily driven, volatile compounds emitted by 21 vulture-damaged objects were collected and analyzed using purge-and-trap gas chromatography/mass spectrometry. Results from these analyses were tabulated and the 27 most commonly occurring compounds were identified. Six of the vulture-damaged samples contained at least 23 of the 27 identified compounds, and hexanal, octanal, undecane, and nonanal were found in all 21 vulture-damaged samples. Using these data, a water-based emulsion containing the 27 most frequently observed compounds in the vulture-damaged samples was formulated to serve as a synthetic materials mimic (SMM). A sustained volatile release delivery system utilizing cellulose sponges was also developed, and used to deliver the SMM for bioassay trials examining the response of captive vultures to the SMM

Photochemistry in the arctic free troposphere: Ozone budget and its dependence on nitrogen oxides and the production rate of free radicals

Abstract. Local ozone production and loss rates for the arctic free troposphere (58–85 ◦ N, 1–6 km, February–May) during the Tropospheric Ozone Production about the Spring Equinox (TOPSE) campaign were calculated using a constrained photochemical box model. Estimates were made to assess the importance of local photochemical ozone production relative to transport in accounting for the springtime maximum in arctic free tropospheric ozone. Ozone production and loss rates from our diel steady-state box model constrained by median observations were first compared to two point box models, one run to instantaneous steady-state and the other run to diel steady-state. A consistent picture of local ozone photochemistry was derived by all three box models suggesting that differences between the approaches were not critical. Our model-derived ozone production rates increased by a factor of 28 in the 1–3 km layer and a factor of 7 in the 3–6 km layer between February and May. The arctic ozone budget required net import of ozone into the arctic free troposphere throughout the campaign; however, the transport term exceeded the photochemical production only in the lower free troposphere (1–3 km) between February and March. Gross ozone production rates were calculated to increase linearly with NOx mixing ratios up to ∼300 pptv in February and for NOx mixing ratio

Coupled evolution of BrOx-ClOx-HOx-NOx chemistry during bromine-catalyzed ozone depletion events in the arctic boundary layer

Extensive chemical characterization of ozone (O3) depletion events in the Arctic boundary layer during the TOPSE aircraft mission in March–May 2000 enables analysis of the coupled chemical evolution of bromine (BrOx), chlorine (ClOx), hydrogen oxide (HOx) and nitrogen oxide (NOx) radicals during these events. We project the TOPSE observations onto an O3 chemical coordinate to construct a chronology of radical chemistry during O3 depletion events, and we compare this chronology to results from a photochemical model simulation. Comparison of observed trends in ethyne (oxidized by Br) and ethane (oxidized by Cl) indicates that ClOxchemistry is only active during the early stage of O3 depletion (O3 \u3e 10 ppbv). We attribute this result to the suppression of BrCl regeneration as O3 decreases. Formaldehyde and peroxy radical concentrations decline by factors of 4 and 2 respectively during O3 depletion and we explain both trends on the basis of the reaction of CH2O with Br. Observed NOx concentrations decline abruptly in the early stages of O3 depletion and recover as O3 drops below 10 ppbv. We attribute the initial decline to BrNO3 hydrolysis in aerosol, and the subsequent recovery to suppression of BrNO3 formation as O3 drops. Under halogen-free conditions we find that HNO4 heterogeneous chemistry could provide a major NOx sink not included in standard models. Halogen radical chemistry in the model can produce under realistic conditions an oscillatory system with a period of 3 days, which we believe is the fastest oscillation ever reported for a chemical system in the atmosphere