The phenomenon of multiculturalism in the regional strategizing

The purpose of the article is to justify the possibility of the modernizing development of the region based on the rational use of the potential of a mixed economy of its territories. In contrast to the existing ideas about multiculturalism as a problem, demonstrating the structural imbalances in the economy of the region, the authors view it as a phenomenon that allows the region to provide a competitive advantage. The methodological base of this study is the convergence of the system, synergistic, institutional and endogenous approaches, and sharing a number of theories - growth poles, territorial industrial clusters, balanced development. The article shows the role of the institutional environment in realizing the potential of a diversified economy in the region. It formulates conceptual positions of the mechanism of regional strategizing aimed at the development of relations between territories with the different types of lifestyles. The basis of the interactions is the consensus of economic interests, compromise of competitive aspirations and market positions of the entities as equal partners. It is concluded that there is the need to change the existing system of regional strategizing in terms of strengthening its focus on the formation of the institutional channels for the communication of corporate, small-scale and farm structures. The recommendations on the potential of the mixed economy of the regions were used in the development of the Strategy of investment development for the Rostov region until 2020

DYNAMICS OF SURFACE EXPRESSION OF TRIGGER RECEPTOR, EXPRESSED BY MYELOID CELLS-1 (TREM-1) ON VARIOUS SUBPOPULATIONS OF MONOCYTES IN EARLY POSTOPERATIVE PERIOD OF DIRECT REVASCULARIZATION OF MYOCARDIUM

We studied surface expression of trigger receptor, expressed by myeloid cells-1 (TREM-1) on various subpopulations of monocytes before direct revascularization of myocardium with artificial blood flow and in early postoperative period. We registered that various expression of TREM-1 on the surface corresponds to subpopulations of monocytes, and the highest level is observed on CD14hiCD16+ monocytes. By the 1st day of postoperative period elevation of surface expression of TREM-1 occurs on all subpopulations of monocytes, testifying to enlargement of their proinflammatory potential

Случай необычного инородного тела пищевода

Abstract Relevance. Esophageal foreign body may be safely removed with rigid esophagoscopy. However, endoscopic methods are not always successful, and this may require a surgery. Aim of study We report a case of an unusual esophageal foreign body.Materials and methods A 34-year-old male patient P. with a foreign body in the esophagus. Endoscopic, radiological, and laboratory diagnostic methods were performed to confirm a foreign body in the esophagus.Results A rare clinical observation of a 34-year-old male patient P. with a foreign body in the esophagus is presented (package with psychoactive substances). After an unsuccessful attempt to remove a foreign body using esophagoscopy, thoracotomy was performed and a foreign body was mechanically brought down into the stomach without opening the lumen of the esophagus. In the postoperative period, the condition was serious, the patient had been in coma for 10 days. There was hectic fever on day 7. On day 9, the left thoracotomy was performed, the revision showed no damage to the esophagus. Urine analysis for psychoactive substances was performed and detected tetrahydrocannabinol acid and aminochlorobenzophenone, a derivative of 1,4-benzodiazepine. On day 9, after repeated surgery, the patient confirmed the fact of swallowing a package of psychoactive substances, being conscious. On day 14 after the second operation, the patient was transferred to the regional narcological hospital.Conclusion In patients with a foreign body in the esophagus in difficult clinical situations, it is necessary to collect anamnesis carefully, apply endoscopic, radiological and laboratory diagnostic methods, including urinalysis for psychoactive substances.Актуальность Инородные тела пищевода (ИТП) могут быть безопасно удалены при ригидной эзофагоскопии, но эндоскопические методы не всегда успешны, что требует проведения оперативного вмешательства.Цель Представить случай необычного ИТП.Материал и методы Пациент Т. 34 лет с ИТП. Для диагностики ИТП выполнены эндоскопические, рентгенологические и лабораторные методы диагностики.Результаты Представлено редкое клиническое наблюдение пациента Т. 34 лет с ИТП (пакет с психоактивными веществами). После безуспешной попытки извлечения инородного тела с помощью эзофагоскопии проведена торакотомия, механическое низведение инородного тела в желудок без вскрытия просвета пищевода. В послеоперационном периоде состояние тяжелое, сознание на уровне комы в течение 10 суток, гектическая лихорадка на 7-е сутки. На 9-е сутки реторакотомия слева, при ревизии повреждений пищевода нет. Проведен анализ мочи на психоактивные вещества (ПАВ), выявлены тетрагидроканнабиноловая кислота, аминохлорбензофенон — производное 1,4-бензодиазепина. На 9-е сутки после повторной операции больной в сознании, подтвердил факт проглатывания пакета с ПАВ. На 14-е сутки после повторной операции больной переведен в областную наркологическую больницу.Заключение У больных с инородным телом пищевода в сложных клинических ситуациях необходимо тщательно собирать анамнез, применять эндоскопические, рентгенологические и лабораторные методы диагностики, в том числе анализ мочи на психоактивные вещества

IMPACT OF BONE MARROW-DERIVED MULTIPOTENT MESENCHYMAL STROMAL CELLS ON SPEED OF POLYCAPROLACTONE AND POLYHYDROXYALKANOATE SCAFFOLDS BIODEGRADATION

The impact of bone marrow-derived multipotent mesenchymal stromal cells of a bone brain (MSCs BB) on the speed of polycaprolactone and polyhydroxyalkanoate scaffolds biodegradation was studied. The presence of cells on the scaffolds surface was found to catalyze their resorption. 2 months after MSCs-covered scaffolds had. been subcutaneously implanted in rats they degraded completely while scaffolds, which had no MSCs cover, had partially resorbed by that time. The obtained results make necessary further studying of MSCs impact mechanisms on biopolymers resorption speed

ISOLATION OF GLICOPROTEID FROM THE FIXED RABIES VIRUS, STRAIN «MOSCOW 3253», AND CONSTRUCTING OF DOT-IMMUNOASSAY DIAGNOSTICUM ON ITS BASIS

Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of conjugation with colloid gold nanoparticles. The diagnosticum is meant for detection of specific antibodies in immune sera of horsesproducers, and in the preparation of anti-rabies immunoglobulin, in dot-immunoassay

Влияние различных вариантов модификации поверхности биодеградируемых пленочных матриксов на адгезию и жизнеспособность мультипотентных мезенхимальных стромальных клеток

Designing a hybrid vascular graft using biocompatible and biodegradable polymers is one of the ways to fill the market with vascular grafts of a small diameter necessary for coronary artery bypass surgery. Good adhesive properties of cultivated cells and low cytotoxicity of biopolymers can improve biocompatibility of polymer-based vascular grafts. The impact of protein modification of membranous polyhydroxyalkanoate and polycaprolactone scaffolds surface on their adhesive properties and cytotoxicity to multipotent mesenchimal stromal cells was evaluated. We used the following compositions of membranous scaffolds: № 1 polyhydroxybutyrate valerate ММ 2 307 kDa with polycaprolactone ММ 80 000 kDa, № 2 had the same composition but was made using a magnetic stirrer, №3 polyhydroxybutyrate ММ 541 kDa with polycaprolactone ММ 80 000 kDa and № 4 polycaprolactone ММ 80 000 kDa only. Premodification of scaffolds surfaces with 75, 50 and 25% fetal calf serum was found to significantly reduce cytotoxicity of scaffold № 4 and improve the adhesive properties of scaffolds № 1, 2 and 3. Premodification with fibronectin (10 µg/ml) mostly improved the adhesive properties of scaffolds № 1—4.Создание гибридного сосудистого графта на основе биосовместимых и биодеградируемых полимеров — один из возможных способов решить проблему отсутствия на рынке сосудистых протезов малого диаметра, необходимых для аортокоронарного шунтирования. Хорошие адгезивные свойства относительно культивируемых клеток и низкая цитотоксичность биополимеров способны улучшить биосовместимость сосудистых графтов на их основе. Исследовано влияние белковой модификации поверхности пленочных матриксов из полиоксиалканоатов и поликапролактона на их адгезивные свойства и цитотоксичность относительно мультипотентных мезенхимальных стромальных клеток. В работе использовались пленочные матриксы (ПМ) следующего состава: ПМ № 1 — полигидроксибутират/валерат с молекулярной массой (ММ) 2 307 кДа с поликапролактоном ММ 80 000 кДа, ПМ № 2 — состав идентичен ПМ № 1, но изготовление матрикса выполнено с помощью магнитной мешалки, ПМ № 3 — полигидроксибутират ММ 541 кДа с поликапролактоном ММ 80 000 кДа и ПМ № 4 — чистый поликапролактон ММ 80 000 кДа. Выявлено, что предварительная модификация поверхностей матриксов 75, 50, 25%-м растворами эмбриональной телячьей сыворотки позволила достоверно снизить цитотоксичность ПМ № 4 и улучшить адгезивные свойства ПМ № 1, 2, 4. Предварительная обработка фибронектином в концентрации 10 мкг/мл в наибольшей степени повлияла на улучшение адгезивных свойств ПМ № 1—4

CB2 Cannabinoid Receptors Contribute to Bacterial Invasion and Mortality in Polymicrobial Sepsis

BACKGROUND:Sepsis is a major healthcare problem and current estimates suggest that the incidence of sepsis is approximately 750,000 annually. Sepsis is caused by an inability of the immune system to eliminate invading pathogens. It was recently proposed that endogenous mediators produced during sepsis can contribute to the immune dysfunction that is observed in sepsis. Endocannabinoids that are produced excessively in sepsis are potential factors leading to immune dysfunction, because they suppress immune cell function by binding to G-protein-coupled CB(2) receptors on immune cells. Here we examined the role of CB(2) receptors in regulating the host's response to sepsis. METHODS AND FINDINGS:The role of CB(2) receptors was studied by subjecting CB(2) receptor wild-type and knockout mice to bacterial sepsis induced by cecal ligation and puncture. We report that CB(2) receptor inactivation by knockout decreases sepsis-induced mortality, and bacterial translocation into the bloodstream of septic animals. Furthermore, CB(2) receptor inactivation decreases kidney and muscle injury, suppresses splenic nuclear factor (NF)-kappaB activation, and diminishes the production of IL-10, IL-6 and MIP-2. Finally, CB(2) receptor deficiency prevents apoptosis in lymphoid organs and augments the number of CD11b(+) and CD19(+) cells during CLP. CONCLUSIONS:Taken together, our results establish for the first time that CB(2) receptors are important contributors to septic immune dysfunction and mortality, indicating that CB(2) receptors may be therapeutically targeted for the benefit of patients suffering from sepsis

Multi-decadal changes in tundra environments and ecosystems: Synthesis of the International Polar Year-Back to the Future Project (IPY-BTF).

Understanding the responses of tundra systems to global change has global implications. Most tundra regions lack sustained environmental monitoring and one of the only ways to document multi-decadal change is to resample historic research sites. The International Polar Year (IPY) provided a unique opportunity for such research through the Back to the Future (BTF) project (IPY project #512). This article synthesizes the results from 13 papers within this Ambio Special Issue. Abiotic changes include glacial recession in the Altai Mountains, Russia; increased snow depth and hardness, permafrost warming, and increased growing season length in sub-arctic Sweden; drying of ponds in Greenland; increased nutrient availability in Alaskan tundra ponds, and warming at most locations studied. Biotic changes ranged from relatively minor plant community change at two sites in Greenland to moderate change in the Yukon, and to dramatic increases in shrub and tree density on Herschel Island, and in sub-arctic Sweden. The population of geese tripled at one site in northeast Greenland where biomass in non-grazed plots doubled. A model parameterized using results from a BTF study forecasts substantial declines in all snowbeds and increases in shrub tundra on Niwot Ridge, Colorado over the next century. In general, results support and provide improved capacities for validating experimental manipulation, remote sensing, and modeling studies

A Case of An Unusual Foreign Body in the Esophagus

Abstract Relevance. Esophageal foreign body may be safely removed with rigid esophagoscopy. However, endoscopic methods are not always successful, and this may require a surgery. Aim of study We report a case of an unusual esophageal foreign body.Materials and methods A 34-year-old male patient P. with a foreign body in the esophagus. Endoscopic, radiological, and laboratory diagnostic methods were performed to confirm a foreign body in the esophagus.Results A rare clinical observation of a 34-year-old male patient P. with a foreign body in the esophagus is presented (package with psychoactive substances). After an unsuccessful attempt to remove a foreign body using esophagoscopy, thoracotomy was performed and a foreign body was mechanically brought down into the stomach without opening the lumen of the esophagus. In the postoperative period, the condition was serious, the patient had been in coma for 10 days. There was hectic fever on day 7. On day 9, the left thoracotomy was performed, the revision showed no damage to the esophagus. Urine analysis for psychoactive substances was performed and detected tetrahydrocannabinol acid and aminochlorobenzophenone, a derivative of 1,4-benzodiazepine. On day 9, after repeated surgery, the patient confirmed the fact of swallowing a package of psychoactive substances, being conscious. On day 14 after the second operation, the patient was transferred to the regional narcological hospital.Conclusion In patients with a foreign body in the esophagus in difficult clinical situations, it is necessary to collect anamnesis carefully, apply endoscopic, radiological and laboratory diagnostic methods, including urinalysis for psychoactive substances