Conservation status of the Georgia Blind Salamander (Eurycea wallacei) from the Floridan Aquifer of Florida and Georgia

The Georgia Blind Salamander (Eurycea wallacei) is a poorly understood stygobitic plethodontid salamander found in the Floridan Aquifer of Florida and Georgia, USA. Its distribution is poorly delimited and little information is available on the ecology and life history of the species. We summarize existing information on the Georgia Blind Salamander and report new findings with regard to distribution, conservation status, and aspects of its ecology and life history from recent surveys in Florida and Georgia, including anecdotal observations made by local cave divers. We also  present data on the prevalence of Batrachochytrium dendrobatidis and discuss potential measures and challenges involved in conservation. Georgia Blind Salamanders are known from at least 35 localities within five United States Geological Survey (USGS) HUC8 watersheds in Florida and Georgia, all within the groundwater of the Upper Floridan Aquifer. Some populations may be large, as cave divers have observed >100 salamanders in a single dive at three localities and reported densities up to 10 salamanders per square meter. We confirm through direct observation that the Dougherty Plain Cave Crayfish (Cambarus cryptodytes) is a predator of E. wallacei. Although we found no evidence that any populations of the Georgia Blind Salamander have been infected by amphibian chytrid fungus, clear threats remain and include: (1) An “at risk” ranking by USGS of the Floridian Aquifer for contamination; (2) overharvesting of groundwater; (3) the presence of an emergent infectious amphibian disease in surface amphibians in the region (e.g., amphibian chytrid fun- gus); and (4) a lack of data on the basic biology and ecology of the species. We recommend the following conservation actions: (1) Better regulations and improved methods for retaining pesticide and fertilizer contaminants on the surface; (2) regulatory review of agricultural crops grown in the region and the establishment of systems and crops that do not require center pivot irrigation; (3) regular monitoring for disease across the range of the species; and (4) establishment of multiple assurance colonies such that captive specimens genetically represent at least half of the known populations

Probability of Regenerating a Normal Limb After Bite Injury in the Mexican Axolotl (\u3cem\u3eAmbystoma mexicanum\u3c/em\u3e)

Multiple factors are thought to cause limb abnormalities in amphibian populations by altering processes of limb development and regeneration. We examined adult and juvenile axolotls (Ambystoma mexicanum) in the Ambystoma Genetic Stock Center (AGSC) for limb and digit abnormalities to investigate the probability of normal regeneration after bite injury. We observed that 80% of larval salamanders show evidence of bite injury at the time of transition from group housing to solitary housing. Among 717 adult axolotls that were surveyed, which included solitary-housed males and group-housed females, approximately half presented abnormalities, including examples of extra or missing digits and limbs, fused digits, and digits growing from atypical anatomical positions. Bite injury likely explains these limb defects, and not abnormal development, because limbs with normal anatomy regenerated after performing rostral amputations. We infer that only 43% of AGSC larvae will present four anatomically normal looking adult limbs after incurring a bite injury. Our results show regeneration of normal limb anatomy to be less than perfect after bite injury

2017 Bat Monitoring along the I-90 Corridor

In this study, the we Passively recorded 28 nights at each of three survey location within Trout Park. Analyzed recordings using 2 auto-classification software programs, Sonobat and Kaleidoscope. Kaleidoscope identified 7,570 files as bat passes. Sonobat identified 9,938 files as bat passes. Identified 4 bat species: Big Brown, Eastern Red, Hoary, and Silver-haired Bats. Kaleidoscope indicated the presence of the Tri-colored Bat in the spring and summer survey periods. Bat populations may be helped by: Increasing the vegetation near the I90 overpass to add more connectivity between the 2 forest patches; Reducing understory clutter from invasive shrubs in the more heavily wooded areas to increase the availability of foraging habitat; Increasing available roosting habitat by maintaining snags and/or erecting bat boxes.Illilnois Toll Highway Authority ITHA RR-15-4228unpublishednot peer reviewe

Cave-adapted evolution in the North American amblyopsid fishes inferred using phylogenomics and geometric morphometrics

© 2020 The Authors. Evolution © 2020 The Society for the Study of Evolution. Cave adaptation has evolved repeatedly across the Tree of Life, famously leading to pigmentation and eye degeneration and loss, yet its macroevolutionary implications remain poorly understood. We use the North American amblyopsid fishes, a family spanning a wide degree of cave adaptation, to examine the impact of cave specialization on the modes and tempo of evolution. We reconstruct evolutionary relationships using ultraconserved element loci, estimate the ancestral histories of eye-state, and examine the impact of cave adaptation on body shape evolution. Our phylogenomic analyses provide a well-supported hypothesis for amblyopsid evolutionary relationships. The obligate blind cavefishes form a clade and the cave-facultative eyed spring cavefishes are nested within the obligate cavefishes. Using ancestral state reconstruction, we find support for at least two independent subterranean colonization events within the Amblyopsidae. Eyed and blind fishes have different body shapes, but not different rates of body shape evolution. North American amblyopsids highlight the complex nature of cave-adaptive evolution and the necessity to include multiple lines of evidence to uncover the underlying processes involved in the loss of complex traits

A new species of Stygobromus Cope, 1872 (Amphipoda, Crangonyctidae) from a hypotelminorheic seepage spring in Washington, D.C., USA

We describe a new species of subterranean amphipod (Amphipoda: Crangonyctidae) in the genus Stygobromus from a hypotelminorheic seepage spring at Shepherd Parkway, part of National Capital East Parks, Washington, D.C., USA, part of the National Park System, using both morphological and genetic approaches. The Anacostia Groundwater Amphipod, S. anacostensis sp. nov. is a member of the S. tenuis species group but differs from related congeners based on body size, serrate blade-like edge of both palms of gnathopods 1 and 2, presence of rastellate setae on the posterodistal margin of the carpus of gnathopod 2, and aspects of the second antennae, mandibular palp, pereopods 5–7, uropods 1 and 2, and telson. Moreover, S. anacostensis sp. nov. is genetically distinct from S. tenuis in the Washington D.C. metropolitan area. The description of S. anacostensis sp. nov. increases the number of described Stygobromus species to eight in the Washington D.C. area and highlights the need for continued biodiversity studies, even in regions that have received considerable attention

Conservation Status and Habitat Use of the West Virginia Spring Salamander (Gyrinophilus Subterraneus) and Spring Salamander (G. Porphyriticus) in General Davis Cave, Greenbrier Co., West Virginia

The West Virginia Spring Salamander (Gyrinophilus subterraneus) is one of four obligate cave-dwelling species of plethodontid salamanders found east of the Mississippi River in the United States. This species is endemic to a single cave system; General Davis Cave, in Greenbrier Co., West Virginia, where it is syntopic with the closely-related Spring Salamander (G. porphyriticus). Accordingly, the West Virginia Spring Salamander is a species of critical conservation concern. Because of it\u27s conservation status and lack of data regarding the ecology and life history, particularly about population trends, we present data on relative abundance of and habitat use by the West Virginia Spring Salamander during a 33-year period from 1975-2008. Specifically we address: (1) stability of the population during the last 33 years; (2) variation in habitat use by life stage and between species (Spring Salamanders and West Virginia Spring Salamanders); (3) plausibility of neoteny in the West Virginia Spring Salamander; and (4) the conservation status of the West Virginia Spring Salamander. We recorded 324 observations of Gyrinophilus salamanders, of which 192 were West Virginia Spring Salamanders, within the study area during 17 surveys. While both larval and metamorphosed West Virginia Spring Salamanders were encountered, only metamorphosed Spring Salamanders were observed. West Virginia Spring Salamander larvae were encountered in pools more often than in riffle habitat. Spring Salamanders were encountered more often in terrestrial habitats versus aquatic habitats. West Virginia Spring Salamanders reach relatively large size before metamorphosing, with some individuals becoming sexual mature as larvae. It remains unknown whether any of these individuals reproduce, however. Although the populations of both species appear to be stable over the past 33 years and not in immediate danger of extinction, the West Virginia Spring Salamander is still in critical conservation concern because of it\u27s extremely restricted distribution and current threats to the cave system it resides in