Getting a Grip on My Depression: How Latina Adolescents Experience, Self-Manage, and Seek Treatment for Depressive Symptoms

Latina (female) adolescents are more likely to experience depressive symptoms and less likely to receive mental health services than their non-Latina White peers. We aimed to develop a framework that explains how Latina adolescents experience, self-manage, and seek treatment for depressive symptoms. Latina young women (n = 25, M age = 16.8 years) who experienced depressive symptoms during adolescence were recruited from clinical and community settings and interviewed about experiences with depressive symptoms. The framework was developed using constructivist grounded theory methods. Participants experienced a psychosocial problem that we labeled being overburdened and becoming depressed. They responded to this problem through a five-phase psychosocial process that we labeled Getting a Grip on My Depression. Family members, peer groups, and mainstream authorities were influential in how participants experienced these phases. Future research should further develop this framework in diverse samples of Latino/a youth. Clinicians can use this framework in discussions with Latina adolescents about depressive symptoms

If I Hear You Correctly: Building and Evaluating Interview Chatbots with Active Listening Skills

Interview chatbots engage users in a text-based conversation to draw out their views and opinions. It is, however, challenging to build effective interview chatbots that can handle user free-text responses to open-ended questions and deliver engaging user experience. As the first step, we are investigating the feasibility and effectiveness of using publicly available, practical AI technologies to build effective interview chatbots. To demonstrate feasibility, we built a prototype scoped to enable interview chatbots with a subset of active listening skills - the abilities to comprehend a user's input and respond properly. To evaluate the effectiveness of our prototype, we compared the performance of interview chatbots with or without active listening skills on four common interview topics in a live evaluation with 206 users. Our work presents practical design implications for building effective interview chatbots, hybrid chatbot platforms, and empathetic chatbots beyond interview tasks.Comment: Working draft. To appear in the ACM CHI Conference on Human Factors in Computing Systems (CHI 2020

Characterizing the Mineralogy of Potential Lunar Landing Sites

Many processes active on the early Moon are common to most terrestrial planets, including the record of early and late impact bombardment. The Moon's surface provides a record of the earliest era of terrestrial planet evolution, and the type and composition of minerals that comprise a planetary surface are a direct result of the initial composition and subsequent thermal and physical processing. Lunar mineralogy seen today is thus a direct record of the early evolution of the lunar crust and subsequent geologic processes. Specifically, the distribution and concentration of specific minerals is closely tied to magma ocean products, lenses of intruded or remelted plutons, basaltic volcanism and fire-fountaining, and any process (e.g. cratering) that might redistribute or transform primary and secondary lunar crustal materials. The association of several lunar minerals with key geologic processes is illustrated in Figure 1. The geologic history of potential landing sites on the Moon can be read from the character and context of local mineralogy

A study of the potential application of digital PCR in the detection of fecal contamination of strawberries using Bacteroides markers

Food-borne illnesses can result from contamination of agricultural products. In this study, we examined nanoplate digital PCR (dPCR) to test for fecal contamination of agricultural products. In nanoplate technique, the PCR mastermix is divided into 8.526,000 partitions, providing direct detection of individual DNA molecules, with correction by Poisson distribution. In this project, strawberries were inoculated with fecal material from animals, and the result detected by nanoplate digital PCR. A detection limit of 250 fg/uL was determined. Overall, dPCR offers a quick and sensitive method to detect contaminated produce

Uncanny Audio:The place and use of sound in Gothic performance

The Phantom of the Opera, the 1986 musical, is a Gothic megahit with a global appeal. Having been performed to audiences around the world, the musical has inspired a multitude of “Phans” to extend the production’s central theme of obsession beyond the auditorium walls and into their intertextual fan practices. That said, such responses stem from a narrative which is ultimately about fandom itself (where the Phantom’s obsession with Christine causes him to haunt, and even murder, the occupants of a fictional Paris opera house). Drawing from musical theatre, popular culture, and fan studies, this chapter argues that there is a direct correlation between the content and presentation of The Phantom of the Opera and the type of fan response it tends to evoke

Whole Genome Sequencing of Newly Established Pancreatic Cancer Lines Identifies Novel Somatic Mutation (<i>c</i>.<i>2587G>A</i>) in Axon Guidance Receptor Plexin A1 as Enhancer of Proliferation and Invasion

<div><p>The genetic profile of human pancreatic cancers harbors considerable heterogeneity, which suggests a possible explanation for the pronounced inefficacy of single therapies in this disease. This observation has led to a belief that custom therapies based on individual tumor profiles are necessary to more effectively treat pancreatic cancer. It has recently been discovered that axon guidance genes are affected by somatic structural variants in up to 25% of human pancreatic cancers. Thus far, however, some of these mutations have only been correlated to survival probability and no function has been assigned to these observed axon guidance gene mutations in pancreatic cancer. In this study we established three novel pancreatic cancer cell lines and performed whole genome sequencing to discover novel mutations in axon guidance genes that may contribute to the cancer phenotype of these cells. We discovered, among other novel somatic variants in axon guidance pathway genes, a novel mutation in the PLXNA1 receptor (c.2587G>A) in newly established cell line SB.06 that mediates oncogenic cues of increased invasion and proliferation in SB.06 cells and increased invasion in 293T cells upon stimulation with the receptor’s natural ligand semaphorin 3A compared to wild type PLXNA1 cells. Mutant PLXNA1 signaling was associated with increased Rho-GTPase and p42/p44 MAPK signaling activity and cytoskeletal expansion, but not changes in E-cadherin, vimentin, or metalloproteinase 9 expression levels. Pharmacologic inhibition of the Rho-GTPase family member CDC42 selectively abrogated PLXNA1 c.2587G>A-mediated increased invasion. These findings provide <i>in-vitro</i> confirmation that somatic mutations in axon guidance genes can provide oncogenic gain-of-function signals and may contribute to pancreatic cancer progression.</p></div

Chromosomal analysis of established cell lines.

<p>Karyograms of cell lines A) SB.06, B) SB.07, C) SB.12, and FISH validation of shown chromosomal translocations in cell lines D) SB.06, E) SB.07.</p

Summary of genomic aberrations in established cell lines.

<p>A) SB.06 and B) SB.07 during WGS displayed as Circos plots of both genomes. The outer circle shows copy number variations (red, gains; green, losses); translocations are depicted as links in the interior of the plot.</p