1,355 research outputs found
Mass spectrometry parameters optimization for the 46 multiclass pesticides determination in strawberries with gas chromatography ion-trap tandem mass spectrometry
Multiclass analysis method was optimized in order to analyze pesticides traces by gas
chromatography with ion-trap and tandem mass spectrometry (GC-MS/MS). The influence of some
analytical parameters on pesticide signal response was explored. Five ion trap mass spectrometry
(IT-MS) operating parameters, including isolation time (IT), excitation voltage (EV), excitation time
(ET),maximum excitation energy or “q” value (q), and isolationmass window (IMW) were numerically
tested in order to maximize the instrument analytical signal response. For this, multiple linear
regression was used in data analysis to evaluate the influence of the five parameters on the analytical
response in the ion trap mass spectrometer and to predict its response. The assessment of the five
parameters based on the regression equations substantially increased the sensitivity of IT-MS/MS in
the MS/MS mode. The results obtained show that for most of the pesticides, these parameters have a
strong influence on both signal response and detection limit.Using the optimized method, a multiclass
pesticide analysis was performed for 46 pesticides in a strawberry matrix. Levels higher than the limit
established for strawberries by the European Union were found in some samples
Usabilidade do sistema de gestão de finanças públicas do Estado do Rio Grande do Sul
Em muitos casos o que compromete a vida de um software não é um problema de funcionalidade, mas as dificuldades de usabilidade. Todo projeto de software que tenha o compromisso de apoiar seus usuários, deveria preocupar-se com a experiência positiva dos mesmos para lidar de forma satisfatória e produtiva com os sistemas computacionais. Tendo como orientação a área de Interação Homem-Computador com ênfase em Usabilidade de Sistemas, o presente trabalho aborda métodos e processos para a avaliação de usabilidade e, com base neles, tem como objetivo apresentar requisitos identificados como sugestões de melhoria da usabilidade para melhorar a interação do Sistema de Gestão de Finanças Públicas do Estado do Rio Grande do Sul (FPE) com seus usuários. Tais requisitos foram apontados pelos próprios usuários que participaram de sessões de avaliação heurística e de testes de usabilidade. Os resultados obtidos indicaram contribuições e evidenciaram a importância da avaliação de usabilidade em projetos de sistemas
Avaliação do modelo de mistura espectral com múltiplos componentes (MESMA) na discriminação de diferentes condições de cobertura em pastagens.
Os objetivos deste trabalho foram: (a) avaliar o potencial do modelo de mistura espectral com múltiplos componentes (multiple endmember spectral mixture analysis - MESMA) para discriminar as condições de cobertura em pastagens (pastagens bem manejadas - A, pastagens em degradação - B e pastagens degradadas- C) a partir de imagens de média resolução espacial; (b) verificar se as métricas de caracterização das pastagens in situ, através da percepção humana, foram realmente capazes de discriminar as diferentes condições de pastagens pré-estabelecidas (A, B e C) e (c) avaliar a possibilidade de utilizar a caracterização de pastagens in situ como calibração e/ou verdade de campo
Impact of phlorotannin extracts from fucus vesiculosus on human gut microbiota
Recent studies indicate that plant polyphenols could be pointed as potential prebiotic candidates since they may interact with the gut microbiota, stimulating its growth and the production of metabolites. However, little is known about the fate of brown seaweeds’ phlorotannins during their passage throughout the gastrointestinal tract. This work aimed to evaluate the stability and bioaccessibility of Fucus vesiculosus phlorotannins after being submitted to a simulated digestive process, as well as their possible modulatory effects on gut microbiota and short-chain fatty acids production following a fermentation procedure using fecal inoculates to mimic the conditions of the large intestine. The stability of phlorotannins throughout the gastrointestinal tract was reduced, with a bioaccessibility index between 2 and 14%. Moreover, slight alterations in the growth of certain commensal bacteria were noticed, with Enterococcus spp. being the most enhanced group. Likewise, F. vesiculosus phlorotannins displayed striking capacity to enhance the levels of propionate and butyrate, which are two important short-chain fatty acids known for their role in intestinal homeostasis. In summary, this work provides valuable information regarding the behavior of F. vesiculosus phlorotannins along the gastrointestinal tract, presenting clear evidence that these compounds can positively contribute to the maintenance of a healthy gastrointestinal condition.info:eu-repo/semantics/publishedVersio
Transthyretin Amyloidosis: Chaperone Concentration Changes and Increased Proteolysis in the Pathway to Disease
Transthyretin amyloidosis is a conformational pathology characterized by the extracellular formation of amyloid deposits and the progressive impairment of the peripheral nervous system. Point mutations in this tetrameric plasma protein decrease its stability and are
linked to disease onset and progression. Since non-mutated transthyretin also forms amyloid in systemic senile amyloidosis and some mutation bearers are asymptomatic throughout their lives, non-genetic factors must also be involved in transthyretin amyloidosis. We
discovered, using a differential proteomics approach, that extracellular chaperones such as fibrinogen, clusterin, haptoglobin, alpha-1-anti-trypsin and 2-macroglobulin are overrepresented in transthyretin amyloidosis. Our data shows that a complex network of extracellular
chaperones are over represented in human plasma and we speculate that they act synergistically to cope with amyloid prone proteins. Proteostasis may thus be as important as point mutations in transthyretin amyloidosis
Phylogeny, ultrastructure, histopathology and prevalence of Myxobolus oliveirai sp. nov., a parasite of Brycon hilarii (Characidae) in the Pantanal wetland, Brazil
This paper presents the morphological, histological and ultrastructural characteristics of Myxobolus oliveirai sp. nov., a parasite of the gill filaments in Brycon hilarii from the Brazilian Pantanal. Out of 216 B. hilariispecimens examined (126 wild and 90 cultivated), 38.1% of wild specimens (n = 48) were infected. The parasites form elongated plasmodia primarily in the tip of gill filaments, reaching about 3 mm in length. A thorough comparison with all the Myxobolus species described from South American hosts, as well as nearly all the Myxobolus species described so far is provided. Partial sequencing of the 18S rDNA gene revealed a total of 1,527 bp. The Myxobolus species parasite of B. hilarii did not match any of the Myxozoa available in GenBank. In the phylogenetic analysis, M. oliveirai sp. nov. composed a monophyletic group with eight other species: five species of Myxobolus parasites of mugilid fishes, two parasites of pangasiid and one of centrarchid. Infection prevalence values of the parasite revealed no significant differences between wet and dry seasons or between males and females. The importance of the infection to the farming of the host species is emphasized.FAPESPCEPTA - ICMBioCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES
Into the Wild: A New Approach to the Aquaculture Production of Brown Trout (Salmo trutta L.) to Enhance Restocking Success
The brown trout (Salmo trutta L.) is one of the most iconic native species from European river
ecosystems and is also one of the main species of interest for recreational fishing activities (e.g., fly
fishing). This species has a significant potential to attract anglers and related investment to the main
fishing grounds, which are usually located in poorly developed areas and away from main city centers.
Due to its environmental and socioeconomic value, this species is often targeted by management
programs directed to the protection and sustainable exploitation of this valuable natural resource.
One of the most common actions to enhance the abundance and condition of trout populations is the
restocking of wild populations with fish from aquaculture facilities. However, most fish come from
fishfarms using production methods such as high densities in the tanks, use of commercial food and
standardized feeding methods, lack of environmental stimulus, and domestication of breeding stocks,
which usually results in poor fitness and very low survival rates after release. This consequently
leads to reduced success of these management actions. To contribute to solving these problems
and enhancing the success of restocking actions for the recovery and sustainable enhancement of
wild trout populations, we propose a novel approach to the production of this species, by testing
and implementing a new protocol that aims to produce wild-reared trout. These fish come from
wild breeders and will be produced with the least human contact in conditions that mimic their
natural habitat. Taking advantage of a recently remodeled and re-equipped aquaculture facility,
located in Central Portugal (Posto Aquícola de Campelo, Figueiró dos Vinhos), we are rearing
trout in low densities (10–20 trout/m3
), like the ones observed in natural habitats, using live food
(larvae and insects), and subjected to environmental stimuli such as refuges, and water and flow
variability, equivalent to those observed in local streams. Accompanied by a pre- and post-restocking
monitoring program (e.g., evaluation of trout abundances and habitat in the target stream, dispersion
and movement patterns and survival), this study will contribute to enhancing the success of future
restocking actions, promoting the sustainable enhancement of wild trout populations, and, thus,
increasing the interest of restocked fishing grounds for angling activities and associated incomes
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