Rapid pre-gel visualization of proteins with mass spectrometry compatibility

Despite all of the prophecies of doom, gel electrophoresis is still prevalent in modern proteomic workflows. However, the currently used protein staining methods represent a serious bottleneck for a quick subsequent protein analysis using mass spectrometry. Substituting traditional protein stains by pre-gel derivatization with visible and mass spectrometry compatible reagents eliminates several processing steps and drastically reduces the sample preparation time. A defined chemistry permits seamless integration of such covalent protein staining methods into standardized bioinformatic pipelines. Using Uniblue A we could covalently stain simple to complex protein samples within 1 minute. Protein profiles on the gels were not compromised and MS/MS based sequence coverages higher than 80% could be obtained. In addition, the visual tracking of covalently stained proteins and peptides facilitates method development and validation. Altogether, this new chemo-proteomic approach enables true "at-line" analysis of proteins

Tailored gamification and serious game framework based on fuzzy logic for saving energy in connected thermostats

Connected thermostats (CTs) often save less energy than predicted because consumers may not know how to use them and may not be engaged in saving energy. Additionally, several models perform contrary to consumers’ expectations and are thus not used the way they are intended to. As a result, CTs save less energy and are underused in households. This paper reviews aspects of gamification and serious games focused on engaging consumers. A gamification and serious games framework is proposed for saving energy that is tailored by a fuzzy logic system to motivate connected thermostat consumers. This intelligent gamification framework can be used to customize the gamification and serious game strategy to each consumer so that fuzzy logic systems can be adapted according to the requirements of each consumer. The framework is designed to teach, engage, and motivate consumers while helping them save electrical energy when using their thermostats. It is described the proposed framework as well as a mockup that can be run on a cellphone. Although this framework is designed to be implemented in CTs, it can be translated to their energy devices in smart homes

Triggered-Lightning Interaction with a Lightning Protective System: Current Distribution and Electromagnetic Environment

A new comprehensive lightning instrumentation system has been designed for Launch Complex 39B (LC3913) at the Kennedy Space Center, Florida. This new instrumentation system includes the synchronized recording of six high-speed video cameras; currents through the nine downconductors of the new lightning protection system for LC3913; four dH/dt, 3-axis measurement stations; and five dE/dt stations composed of two antennas each. A 20:1 scaled down model of the new Lightning Protection System (LPS) of LC39B was built at the International Center for Lightning Research and Testing, Camp Blanding, FL. This scaled down lightning protection system was instrumented with the transient recorders, digitizers, and sensors to be used in the final instrumentation installation at LC3913. The instrumentation used at the ICLRT is also a scaled-down instrumentation of the LC39B instrumentation. The scaled-down LPS was subjected to seven direct lightning strikes and six (four triggered and two natural nearby flashes) in 2010. The following measurements were acquired at the ICLRT: currents through the nine downconductors; two dl-/dt, 3-axis stations, one at the center of the LPS (underneath the catenary wires), and another 40 meters south from the center of the LPS; ten dE/dt stations, nine of them on the perimeter of the LPS and one at the center of the LPS (underneath the catenary wire system); and the incident current. Data from representative events are presented and analyzed in this paper

Evaluation of the Performance Characteristics of CGLSS II and U.S. NLDN Using Ground-Truth Dalta from Launch Complex 398, Kennedy Space Center, Florida

A new comprehensive lightning instrumentation system has been designed for Launch Complex 39B (LC39B) at the Kennedy Space Center, Florida. This new instrumentation system includes seven synchronized high-speed video cameras, current sensors installed on the nine downconductors of the new lightning protection system (LPS) for LC39B; four dH/dt, 3-axis measurement stations; and five dE/dt stations composed of two antennas each. The LPS received 8 direct lightning strikes (a total of 19 strokes) from March 31 through December 31 2011. The measured peak currents and locations are compared to those reported by the Cloud-to-Ground Lightning Surveillance System (CGLSS II) and the National Lightning Detection Network (NLDN). Results of comparison are presented and analyzed in this paper

Multifractal wave functions of simple quantum maps

We study numerically multifractal properties of two models of one-dimensional quantum maps, a map with pseudointegrable dynamics and intermediate spectral statistics, and a map with an Anderson-like transition recently implemented with cold atoms. Using extensive numerical simulations, we compute the multifractal exponents of quantum wave functions and study their properties, with the help of two different numerical methods used for classical multifractal systems (box-counting method and wavelet method). We compare the results of the two methods over a wide range of values. We show that the wave functions of the Anderson map display a multifractal behavior similar to eigenfunctions of the three-dimensional Anderson transition but of a weaker type. Wave functions of the intermediate map share some common properties with eigenfunctions at the Anderson transition (two sets of multifractal exponents, with similar asymptotic behavior), but other properties are markedly different (large linear regime for multifractal exponents even for strong multifractality, different distributions of moments of wave functions, absence of symmetry of the exponents). Our results thus indicate that the intermediate map presents original properties, different from certain characteristics of the Anderson transition derived from the nonlinear sigma model. We also discuss the importance of finite-size effects.Comment: 15 pages, 21 figure

Evaluation of the Performance Characteristics of CGLSS II and U.S. NLDN Using Ground-Truth Data from Launch Complex 398, Kennedy Space Center, Florida

A new comprehensive lightning instrumentation system has been designed for Launch Complex 39B (LC39B) at the Kennedy Space Center, Florida. This new instrumentation system includes six synchronized high-speed video cameras, current sensors installed on the nine downcouductors of the new lightning protection system (LPS) for LC39B; four dH/dt, 3-axis measurement stations; and five dE/dt stations composed of two antennas each. The LPS received 8 direct lightning strikes (a total of 19 strokes) from March 31 through December 31, 2011. The measured peak currents and locations are compared to those reported by the CGLSS 11 and the NLDN. Results of comparison are presented and analyzed in this paper

Evaluation of Lightning Incidence to Elements of a Complex Structure: A Monte Carlo Approach

There are complex structures for which the installation and positioning of the lightning protection system (LPS) cannot be done using the lightning protection standard guidelines. As a result, there are some "unprotected" or "exposed" areas. In an effort to quantify the lightning threat to these areas, a Monte Carlo statistical tool has been developed. This statistical tool uses two random number generators: a uniform distribution to generate origins of downward propagating leaders and a lognormal distribution to generate returns stroke peak currents. Downward leaders propagate vertically downward and their striking distances are defined by the polarity and peak current. Following the electrogeometrical concept, we assume that the leader attaches to the closest object within its striking distance. The statistical analysis is run for 10,000 years with an assumed ground flash density and peak current distributions, and the output of the program is the probability of direct attachment to objects of interest with its corresponding peak current distribution

Evaluation of Grounding Impedance of a Complex Lightning Protective System Using Earth Ground Clamp Measurements and ATP Modeling

A new Lightning Protection System (LPS) was designed and built at Launch Complex 39B (LC39B), at the Kennedy Space Center (KSC), Florida, which consists of a catenary wire system (at a height of about 181 meters above ground level) supported by three insulators installed atop three towers in a triangular configuration. A total of nine downconductors (each about 250 meters long, on average) are connected to the catenary wire system. Each of the nine downconductors is connected to a 7.62-meter radius circular counterpoise conductor with six equally spaced 6-meter long vertical grounding rods. Grounding requirements at LC39B call for all underground and above ground metallic piping, enclosures, raceways, and cable trays, within 7.62 meters of the counterpoise, to be bounded to the counterpoise, which results in a complex interconnected grounding system, given the many metallic piping, raceways, and cable trays that run in multiple direction around LC39B. The complexity of this grounding system makes the fall of potential method, which uses multiple metallic rods or stakes, unsuitable for measuring the grounding impedances of the downconductors. To calculate the downconductors grounding impedance, an Earth Ground Clamp (a stakeless grounding resistance measuring device) and a LPS Alternative Transient Program (ATP) model are used. The Earth Ground Clamp is used to measure the loop impedance plus the grounding impedance of each downconductor and the ATP model is used to calculate the loop impedance of each downconductor circuit. The grounding impedance of the downconductors is then calculated by subtracting the ATP calculated loop impedances from the Earth Ground Clamp measurements

Semiclassical approach to fidelity amplitude

The fidelity amplitude is a quantity of paramount importance in echo type experiments. We use semiclassical theory to study the average fidelity amplitude for quantum chaotic systems under external perturbation. We explain analytically two extreme cases: the random dynamics limit --attained approximately by strongly chaotic systems-- and the random perturbation limit, which shows a Lyapunov decay. Numerical simulations help us bridge the gap between both extreme cases.Comment: 10 pages, 9 figures. Version closest to published versio

A New Lightning Instrumentation System for Pad 39B at the Kennedy Space Center Florida

This viewgraph presentation describes a new lightning instrumentation system for pad 39B at Kennedy Space Center Florida. The contents include: 1) Background; 2) Instrumentation; 3) Meteorological Instrumentation; and 4) Lessons learned. A presentation of the data acquired at Camp Blanding is also shown