Thymine starvation causes abortive initiation of chromosome replication critical for cell death in Escherichia coli

Presentado en: XXXVI Congreso SEBBM (Sociedad Española de Bioquímica y Biología Molecular), Madrid, 4-6 septiembre, 2013Thymineless death (TLD), a phenomenon in which thymine auxotrophy becomes lethal when cells are starved for thymine, can be prevented by rifampicin addition. In this work, we have obtained evidence indicating that among the effects of RNA polymerase inhibition by rifampicin, the inhibition of chromosomal replication initiation is the process responsible for TLD suppression. We show that diminishing, abolishing or increasing the transcription level around oriC alleviates TLD by limiting the chromosomal initiation capacity (ChIC) generated under thymine starvation. TLD suppression was eliminated by DnaA inactivation or the deletion in the left half of the replication origin or deletion of the DnaA-boxes located in the right half of the oriC region. In accordance with these data, two-dimensional DNA electrophoresis gels showed an accumulation of simple-Y and bubble arc replication intermediates, as well as recombination structures at the oriC region during thymine starvation. None of these structures were observed under genetic or physiological conditions that suppress TLD. These results demonstrate that abortive initiations of chromosome replication occur under thymine starvation, supporting the idea that these events are a critical target for the lethal damage caused by thymine starvation and that results in TLD.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Du Nord québécois au Nord européen. Le regard de Nicole Brossard

Nicole Brossard termine son essai L’horizon du fragment (2004) en énonçant: "Je suis là. Au bout du monde, [...]. Les pieds dans la boue de l’été lumineux de l’Arctique, je regarde devant moi." Nous y retrouvons des traits fonciers de son œuvre, à savoir l’omniprésence du regard, des images visuelles, de la lumière. Après une analyse des sensations et des représentations associées à la nordicité, nous étudierons la place de la lumière et des images visuelles dans son œuvre: des images-paysages nordiques dont des paysages urbains (Montréal, Québec, Stockholm) et le Grand Nord. Des images du Nord européen et québécois, construites par le regard

The role of group identification in the well-being of Spaniards with gypsy ethnicity

This is an Accepted Manuscript of an article published by Taylor & Francis in The Journal of Social Psychology. Gomez Berrocal, M. D. C., Porras, C., & Mata, S. (2020). The role of group identification in the well-being of Spaniards with gypsy ethnicity. The Journal of Social Psychology, 160(2), 204-215 which has been published in final form at: https://doi.org/10.1080/00224545.2019.1634504 It is deposited under the terms of the Creative Commons Attribution-Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.”Objective: To describe the role of sex, age, educational level and psychosocial group-identification factors in well-being and satisfaction with life. Method: 229 Spanish Gypsies completed a survey of demographic data, psychological well- being, life satisfaction, ethnic identity and the individual’s inclusion of self within the ingroup. Results: (a) only level of studies is related to satisfaction with life; (b) participants with higher scores in ethnic identity reported more well-being and more life satisfaction; and (c) assessment of ethnic belonging affects more areas of well-being than does perception of closeness to the ingroup. Conclusion: objective conditions of deprivation are not related to well-being as reported by the participants; it is important to study how Spanish Gypsies value and perceive their ethnicity in order to predict their well-being and satisfaction with life.Departamento de Psicología Social (Universidad de Granada), Departamento de Metodología de las Ciencias del Comportamiento (Universidad de Granada), Departamento de Personalidad, Evaluación y Tratamiento Psicológico (Universidad de Granada)