42 research outputs found
R-matrix calculations of electron impact electronic excitation of BeH
The R-matrix method is used to perform high-level calculations of electron collisions with beryllium mono-hydride at its equilibrium geometry with a particular emphasis on electron impact electronic excitation. Several target and scattering models are considered. The calculations were performed using (1) the UKRMol suite which relies on the use of Gaussian type orbitals (GTOs) to represent the continuum and (2) using the new UKRMol+ suite which allows the inclusion of B-spline type orbitals in the basis for the continuum. The final close-coupling scattering models used the UKRMol+ code and a frozen core, valence full configuration interaction, method based on a diffuse GTO atomic basis set. The calculated electronic properties of the molecule are in very good agreement with state-of-the-art electronic structure calculations. The use of the UKRMol+ suite proved critical since it allowed the use of a large R-matrix sphere (35 Bohr), necessary to contain the diffuse electronic states of the molecule. The corresponding calculations using UKRMol are not possible due to numerical problems associated with the combination of GTO-only continuum and a large R-matrix sphere. This work provides the first demonstration of the utility and numerical stability of the new UKRMol+ code. The inelastic cross sections obtained here present a significant improvement over the results of earlier studies on BeH
Low energy inelastic electron scattering from carbon monoxide: I. Excitation of the aÂł Î , a'Âł ÎŁ âș and AÂč Î electronic states
Differential scattering cross sections for electron excitation of the three lowest excited electron states of carbon monoxide are obtained experimentally using low-energy electron energy-loss spectroscopy and theoretically using the R-matrix method. The incident electron energies range from near-threshold of 6.3 eV to 20 eV. Experimental scattering angles range from 20° to 120°. The normalization of the experimental cross sections is made to available experimental elastic scattering data (Gibson et al 1996 J. Phys. B: At. Mol. Opt. Phys. 29 3197). The R-matrix calculations use three distinct close-coupling models and their results are compared to available experimental and theoretical cross sections. The overall comparison leads to significantly improved description of the excitation cross sections for this target
Neurochemical Changes in the Mouse Hippocampus Underlying the Antidepressant Effect of Genetic Deletion of P2X7 Receptors.
Recent investigations have revealed that the genetic deletion of P2X7 receptors (P2rx7) results in an antidepressant phenotype in mice. However, the link between the deficiency of P2rx7 and changes in behavior has not yet been explored. In the present study, we studied the effect of genetic deletion of P2rx7 on neurochemical changes in the hippocampus that might underlie the antidepressant phenotype. P2X7 receptor deficient mice (P2rx7-/-) displayed decreased immobility in the tail suspension test (TST) and an attenuated anhedonia response in the sucrose preference test (SPT) following bacterial endotoxin (LPS) challenge. The attenuated anhedonia was reproduced through systemic treatments with P2rx7 antagonists. The activation of P2rx7 resulted in the concentration-dependent release of [3H]glutamate in P2rx7+/+ but not P2rx7-/- mice, and the NR2B subunit mRNA and protein was upregulated in the hippocampus of P2rx7-/- mice. The brain-derived neurotrophic factor (BDNF) expression was higher in saline but not LPS-treated P2rx7-/- mice; the P2rx7 antagonist Brilliant blue G elevated and the P2rx7 agonist benzoylbenzoyl ATP (BzATP) reduced BDNF level. This effect was dependent on the activation of NMDA and non-NMDA receptors but not on Group I metabotropic glutamate receptors (mGluR1,5). An increased 5-bromo-2-deoxyuridine (BrdU) incorporation was also observed in the dentate gyrus derived from P2rx7-/- mice. Basal level of 5-HT was increased, whereas the 5HIAA/5-HT ratio was lower in the hippocampus of P2rx7-/- mice, which accompanied the increased uptake of [3H]5-HT and an elevated number of [3H]citalopram binding sites. The LPS-induced elevation of 5-HT level was absent in P2rx7-/- mice. In conclusion there are several potential mechanisms for the antidepressant phenotype of P2rx7-/- mice, such as the absence of P2rx7-mediated glutamate release, elevated basal BDNF production, enhanced neurogenesis and increased 5-HT bioavailability in the hippocampus
Rod and Cone Pathway Signalling Is Altered in the P2X7 Receptor Knock Out Mouse
The P2X7 receptor (P2X7-R) is expressed in the retina and brain and has been implicated in neurodegenerative diseases. However, whether it is expressed by neurons and plays a role as a neurotransmitter receptor has been the subject of controversy. In this study, we first show that the novel vesicular transporter for ATP, VNUT, is expressed in the retina, verifying the presence of the molecular machinery for ATP to act as neurotransmitter at P2X7-Rs. Secondly we show the presence of P2X7-R mRNA and protein in the retina and cortex and absence of the full length variant 1 of the receptor in the P2X7-R knock out (P2X7-KO) mouse. The role of the P2X7-R in neuronal function of the retina was assessed by comparing the electroretinogram response of P2X7-KO with WT mice. The rod photoreceptor response was found to be similar, while both rod and cone pathway post-photoreceptor responses were significantly larger in P2X7-KO mice. This suggests that activation of P2X7-Rs modulates output of second order retinal neurons. In line with this finding, P2X7-Rs were found in the outer plexiform layer and on inner retinal cell classes, including horizontal, amacrine and ganglion cells. The receptor co-localized with conventional synapses in the IPL and was expressed on amacrine cells post-synaptic to rod bipolar ribbon synapses. In view of the changes in visual function in the P2X7-KO mouse and the immunocytochemical location of the receptor in the normal retina, it is likely the P2X7-R provides excitatory input to photoreceptor terminals or to inhibitory cells that shape both the rod and cone pathway response
P2RX7 Purinoceptor: A Therapeutic Target for Ameliorating the Symptoms of Duchenne Muscular Dystrophy
open access articleDuchenne muscular dystrophy (DMD) is the most common inherited muscle disease, leading to severe disability and death in young men. Death is caused by the progressive degeneration of striated muscles aggravated by sterile inflammation. The pleiotropic effects of the mutant gene also include cognitive and behavioral impairments and low bone density.
Current interventions in DMD are palliative only as no treatment improves the long-term
outcome. Therefore, approaches with a translational potential should be investigated, and
key abnormalities downstream from the absence of the DMD product, dystrophin, appear to be strong therapeutic targets. We and others have demonstrated that DMD mutations alter ATP signaling and have identified P2RX7 purinoceptor up-regulation as being responsible for the death of muscles in the mdx mouse model of DMD and human DMD lymphoblasts. Moreover, the ATPâP2RX7 axis, being a crucial activator of innate immune responses, can contribute to DMD pathology by stimulating chronic inflammation. We investigated whether ablation of P2RX7 attenuates the DMD model mouse phenotype to assess receptor suitability as a therapeutic target
Molecular and functional properties of P2X receptorsârecent progress and persisting challenges
Synthesis and Characterization of Highly Fluorinated Graphite Containing sp<sup>2</sup> and sp<sup>3</sup> Carbon
International audienceHighly fluorinated graphites were obtained by reaction of fluorine with graphite, at room temperature, in the presence of iodine pentafluoride and hydrogen fluoride. The re-fluorination between 100 and 600 °C yielded a series of fluorinated graphites with semi-ionic and covalent CâF bonds with varying amounts of sp2- and sp3-type carbon hybridization. Three types of materials have been generated: for re-fluorination lower than 400 °C, the CâF bond is still mainly semi-ionic and the carbon atoms are sp2 coplanar; for re-fluorination temperatures between 400 and 550 °C, âhybridâ graphite fluorides characterized by two types of CâF bonds, semi-ionic and covalent, with sp2 and sp3 carbon hybridization were found. For higher re-fluorination temperatures (between 550 and 600 °C), despite a similar fluorination amount and a covalent CâF bond, the sp2 character never completely vanished even at a high re-fluorination temperature of 600 °C. At such a temperature, a directly fluorinated graphite would be of the pure sp3 type
Tuning the discharge potential of fluorinated carbon used as electrode in primary lithium battery
International audienceWhen the carbon lattice of the starting material exhibits a curvature as in the case of fullerenes and derivatives, the C-F bonding in the compounds resulting from the fluorination using molecular fluorine is strongly affected and the covalence is significantly weakened. 19F solid state NMR underlines that the higher the curvature the lower the C-F bonding covalence is. The electrochemical discharge potentials of these materials used as electrode in primary lithium batteries can be then tuned according to the curvature, i.e. the diameter of the outer tubes. The potential decreases from 2.9 to 2.3 V versus Li+/Li° according to the following classification: SWCNTs, MWCNTs and carbon nanofibres, which can be described as MWCNTs with large diameter due to the high number of walls. The case of highly fluorinated fullerenes is different because several types of bonding coexist and are progressively broken during the electrochemical process at potentials ranged in between 2.0 and 3.6 V. The behaviour of fluorinated nanocarbons is also compared to conventional graphite fluoride
Cyclosporine-A Induced Gingival Overgrowth
Abstract Background. The link between the gingival overgrowth and cyclosporine pharmacokinetical variables, especially cyclosporine doses which appear to act as stimulator of the gingival proliferative changes, presents a field of interest of large number of researches. The existence of undefined association and/or interaction between the cyclosporine and periodontal variables, could be responsible for this type of gingival overgrowth. The aim of this study was to examine the correlation between the degree of gingival overgrowth, daily doses of cyclosporine A and parodontal parameters. Methods. 120 patients with renal transplants were included in this examination. The cohort was divided into a four groups according to the daily dose of cyclosporine (100, 125, 150, 175 mg). The degree of gingival overgrowth (GOI) was investigated, using a MacGaw index. The plaque index (PI), apical migration, total daily doses of cyclosporine and plasma concentration, was recorded for various groups and a prospective longitudinal follow -up was conducted. Results. Statistically significant correlation was found between GOI and cyclosporine dose ( =0,3; p< 0,01) and also with dental plaque ( = 0,6; p<0,01), gingival inflammation ( =0,3; p<0,01) and lost attachment ( = 0,1; p<0,05). The lost attachment varied significantly between groups, (p<0,05). Gingival inflammation index (GII) also differed among groups with different dose (p<0,01). Our findings showed differences in gingival overgrowth index between groups (p< 0,05). Conclusions. Our results show a positive correlation between gingival overgrowth and pharmacological parameters, especially the high doses (above 175 mg) of cyclosporine and also with parodontal parameters which lead to parodontal destructions. Additionally, we underlined the effect of local factors as a cofactor in the development of this side effect