Genes Involved in Viral Carcinogenesis and Tumor Initiation in Hepatitis C Virus–Induced Hepatocellular Carcinoma

The role of chronic hepatitis C virus (HCV) in the pathogenesis of HCV-associated hepatocellular carcinoma (HCC) remains controversial. To understand the transition from benign to malignant, we studied the gene expression patterns in liver tissues at different stages, including normal, cirrhosis, and different HCC stages. We studied 108 liver tissue samples obtained from 88 distinct patients (41 HCV-cirrhotic tissues, 17 HCV-cirrhotic tissues from patients with HCC, and 47 HCV-HCC tissues). Differentially expressed genes (DEG) were studied by use of high-density oligonucleotide arrays. Among probe sets identified as differentially expressed via the F test, all pairwise comparisons were performed. Cirrhotic tissues with and without concomitant HCC were further evaluated, and a classifier was used to predict whether the tissue type was associated with HCC. Differential expression profiles were analyzed using Interaction Networks and Functional Analysis. Characteristic gene signatures were identified when normal tissue was compared with cirrhosis, cirrhosis with early HCC, and normal with HCC. Pathway analysis classified the cellular and biological functions of the DEG as related to cellular growth and proliferation, cell death and inflammatory disease in cirrhosis; cell death, cell cycle, DNA replication, and immune response in early HCCs; and cell death, cell growth and proliferation, cell cycle, and DNA repair in advanced HCCs. Characteristic gene signatures were identified at different stages of HCV-HCC progression. A set of genes were identified to predict whether the cirrhotic tissue was associated with HCC

Análisis de la relación entre la planeación estratégica, actividades de producción y operaciones, aseguramiento de calidad y comercialización de las PYMES del sector manufactura dedicadas a la fabricación de prendas de vestir de Lima Metropolitana, 2021

Hoy en día, en el Perú, las PYMES componen el principal motor de la economía peruana tanto en crecimiento económico de cara al PBI y como generador de empleado para millones de peruanos. Este estudio se ha realizado con el objetivo de poder identificar aspectos de mejoras en las PYMES para que puedan explotar de mejor manera las áreas estratégicas o críticas para el negocio y puedan aumentar su competitividad en el mercado. Por ello, la presente investigación busca evaluar la relación que existe entre ciertas actividades y áreas funcionales, entre ellas, la planeación estratégica, actividades de producción y operaciones, aseguramiento de calidad y comercialización de las PYMES en el sector manufactura de Lima Metropolitana, en el 2021. La metodología empleada en esta investigación fue cuantitativa probabilístico aleatorio simple, el cual sirve para investigaciones de carácter cuantitativo, que cuentan con una población delimitada. Para ello, se elaboró un instrumento con 46 preguntas con distintas variables que conforman estas dimensiones, mediante la escala de Likert. El mismo que fue aplicado sobre toda la muestra de 121 encargados de altos mandos de las PYMES para el sector estudiado entre el año 2021 y 2022.Nowadays, in Peru, SMEs are the main engine of the Peruvian economy, both in terms of economic growth in terms of GDP and as a generator of employment for millions of Peruvians. This study has been carried out with the objective of identifying aspects of improvement in SMEs so that they can better exploit the strategic or critical areas for the business and increase their competitiveness in the market. Therefore, this research seeks to evaluate the relationship between certain activities and functional areas, including strategic planning, production and operations activities, quality assurance and marketing of SMEs in the manufacturing sector of Metropolitan Lima, in 2021. The methodology used in this research was quantitative, simple randomized probabilistic, which is used for quantitative research with a delimited population. For this purpose, an instrument was developed with 46 questions with different variables that make up these dimensions, using the Likert scale. It was applied to the entire sample of 121 top managers of SMEs in the sector studied between 2021 and 2022.Tesi

Uso de antimicrobianos y percepción de riesgo de resistencia antimicrobiana en veterinarios. Buenos Aires, Argentina. (Resultados preliminares)

Comunicación científica en formato E- PÓSTER, realizada en las III Jornadas Internacionales de Investigación, Ciencia y Universidad y las XII Jornadas de Investigación UMaza, en el Bloque de comunicaciones científicas: "MEDICINA VETERINARIA EN PEQUEÑOS ANIMALES", el mismo fue moderado por el Vet. MARTÍN QUERO. Las jornadas se llevaron adelante desde 19 al 23 de octubre del 2020 en formato totalmente virtual bajo plataforma Zoom y fueron transmitidas por el canal YouTube de la UMaza y el Facebook del Área de Ciencia y Técnica UMaza (Somos Ciencia y Técnica UMaza)

Reduced Expression of Inflammatory Genes in Deceased Donor Kidneys Undergoing Pulsatile Pump Preservation

Background The use of expanded criteria donor kidneys (ECD) had been associated with worse outcomes. Whole gene expression of pre-implantation allograft biopsies from deceased donor kidneys (DDKs) was evaluated to compare the effect of pulsatile pump preservation (PPP) vs. cold storage preservation (CSP) on standard and ECD kidneys. Methodology/Principal Findings 99 pre-implantation DDK biopsies were studied using gene expression with GeneChips. Kidneys transplant recipients were followed post transplantation for 35.8 months (range = 24–62). The PPP group included 60 biopsies (cold ischemia time (CIT) = 1,367+/−509 minutes) and the CSP group included 39 biopsies (CIT = 1,022+/−485 minutes) (P Conclusions/Significance Inflammation was the most important up-regulated pattern associated with pre-implantation biopsies undergoing CSP even when the PPP group has a larger number of ECD kidneys. No significant difference was observed in delayed graft function incidence and graft function post-transplantation. These findings support the use of PPP in ECD donor kidneys

Biomarkers of disease differentiation: HCV recurrence versus acute cellular rejection

The wound-healing process induced by chronic hepatitis C virus (HCV) infection triggers liver damage characterized by fibrosis development and finally cirrhosis. Liver Transplantation (LT) is the optimal surgical treatment for HCV-cirrhotic patients at end-stage liver disease. However, acute cellular rejection (ACR) and HCV recurrence disease represent two devastating complications post-LT. The accurate differential diagnosis between both conditions is critical for treatment choice, and similar histological features represent a challenge for pathologists. Moreover, the HCV recurrence disease severity is highly variable post-LT. HCV recurrence disease progression is characterized by an accelerated fibrogenesis process, and almost 30% of those patients develop cirrhosis at 5-years of follow-up. Whole-genome gene expression (WGE) analyses through well-defined oligonucleotide microarray platforms represent a powerful tool for the molecular characterization of biological process. In the present manuscript, the utility of microarray technology is applied for the ACR and HCV-recurrence biological characterization in post-LT liver biopsy samples. Moreover, WGE analysis was performed to identify predictive biomarkers of HCV recurrence severity in formalin-fixed paraffin-embedded liver biopsies prospectively collected

Shampoo Sólido: Eko Care

El presente proyecto tiene como creación a la idea de negocio llamada Eko-Care con la finalidad de ofrecer un producto innovador y ecoamigable. El shampoo sólido será un producto diferenciado en nuestro mercado objetivo, ya que queremos promover un estilo de vida y cuidado personal sostenible. Se considera que el mercado objetivo son mujeres entre 18 y 39 años de edad de Lima metropolitana pertenecientes a los sectores económicos A y B con afinidad ecológica y cuidado personal, porque creemos que cuentan con la disposición de pago al valor agregado que tiene nuestros shampoos sólidos. Asimismo, Ekocare cuenta con tres tipos de shampoo: Tinturado, Todo Tipo y Anticaspa y Anticaída con dos presentaciones; simple y box. El plan de marketing que Ekocare empleara a sus clientes potenciales son campañas y promociones mensuales mediante ventas online de todas las redes sociales y ferias. Asimismo, se busca el posicionamiento de la marca y el cumplimiento de los objetivos. El plan de operaciones se estableció en el macroproceso diversas actividades claves enfocados en cumplir con nuestros clientes manteniendo una adecuada relación con los proveedores y la calidad del producto. En el plan financiero, la inversión inicial será S/26 000 soles donde el 70% aportado por los socios del negocio y el 30% por el nuevo inversor para Eko-care.The present project has as its creation the business idea called Eko-Care in order to offer an innovative and eco-friendly product. The solid shampoo will be a differentiated product in our target market, since we want to promote a sustainable lifestyle and personal care. The target market is considered to be women between 18 and 39 years of age in metropolitan Lima belonging to economic sectors A and B with ecological affinity and personal care, because we believe that they have the provision of payment to the added value that our solid shampoos have . Likewise, Ekocare has three types of shampoo: Dyeing, All Kinds and Anti-Dandruff and Fall Protection with two presentations; simple and box. The marketing plan that Ekocare will employ its potential clients are monthly campaigns and promotions through online sales of all social networks and fairs. Likewise, the positioning of the brand and the fulfillment of the objectives are sought. The operations plan was established in the macroprocess several key activities focused on meeting our customers maintaining an adequate relationship with suppliers and product quality. In the financial plan, the initial investment will be S / 26,000 soles where 70% contributed by the business partners and 30% by the new investor for Eko-care.Trabajo de investigació

Genetic inhibition of hepatic acetyl-CoA carboxylase activity increases liver fat and alters global protein acetylationa

Lipid deposition in the liver is associated with metabolic disorders including fatty liver disease, type II diabetes, and hepatocellular cancer. The enzymes acetyl-CoA carboxylase 1 (ACC1) and ACC2 are powerful regulators of hepatic fat storage; therefore, their inhibition is expected to prevent the development of fatty liver. In this study we generated liver-specific ACC1 and ACC2 double knockout (LDKO) mice to determine how the loss of ACC activity affects liver fat metabolism and whole-body physiology. Characterization of LDKO mice revealed unexpected phenotypes of increased hepatic triglyceride and decreased fat oxidation. We also observed that chronic ACC inhibition led to hyper-acetylation of proteins in the extra-mitochondrial space. In sum, these data reveal the existence of a compensatory pathway that protects hepatic fat stores when ACC enzymes are inhibited. Furthermore, we identified an important role for ACC enzymes in the regulation of protein acetylation in the extra-mitochondrial space

DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis

Fascioliasis is a highly pathogenic zoonotic disease emerging in recent decades, in part due to the effects of climate and global changes. South America is the continent presenting more numerous human fascioliasis endemic areas and the highest Fasciola hepatica infection prevalences and intensities known in humans. These serious public health scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks. To understand this difference, we characterized F. hepatica from cattle and horses and lymnaeids of Uruguay by sequencing of ribosomal DNA ITS-2 and ITS-1 spacers and mitochondrial DNA cox1, nad1 and 16S genes. Results indicate that vectors belong to Lymnaea neotropica instead of to Lymnaea viator, as always reported from Uruguay. Our correlation of fasciolid and lymnaeid haplotypes with historical data on the introduction and spread of livestock species into Uruguay allow to understand the molecular diversity detected. We study the life cycle and transmission features of F. hepatica by L. neotropica of Uruguay under standardized experimental conditions to enable a comparison with the transmission capacity of F. hepatica by Galba truncatula at very high altitude in Bolivia. Results demonstrate that although L. neotropica is a highly efficient vector in the lowlands, its transmission capacity is markedly lower than that of G. truncatula in the highlands. On this baseline, we review the human fascioliasis cases reported in Uruguay and analyze the present and future risk of human infection in front of future climate change estimations