The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies

The purpose of this study was to investigate the cholesterol removal by some bifidobacterium spp. at in vitro and in vivo conditions with the emphasis of their bile salt deconjugation ability. Bile salt hydrolase (BSH) activity, which is the measurement of enzyme activity responsible for bile salt deconjugation, was quantified by high pressure liquid chromatography (HPLC) assay. B. in/antis G001204 was the isolate with the highest deconjugation rate in TPY broth supplemented with 5mM GCDC. Generally all the isolates deconjugated glycoconjugated bile acid in higher amount (P<0.05) compared to tauroconjugates. Likewise in overall the percentage of deconjugation activity was higher in TPY medium supplemented with 5mM bile acids (P<0.05) compared to the TPY broth with 10mM bile acids. Cholesterol removal from media was strain-dependent. The percentage of cholesterol assimilated in TPY containing 0.52mM cholesterol plus bile acids ranged from 4.0% for B. infantis F41134 to 47.0% for B. infantis G001204. The presence of bile salt was prerequisite for cholesterol removal.Results of the in vivo experiment showed that total cholesterol concentration in rats fed on the high-cholesterol diet plus either B. in/antis 0001204 or B. animalis ATCC 27672 in a 2-week period were significantly (P<0.05) lower than the control group. Total fecal bile acid excretion increased in animal groups throughout the high-cholesterol diet feeding and probiotic-treated groups had higher excretion rate of fecal bile acids compared to the control significantly (P<0.05)

Generic Structure of Discussion Sections in ESP Research Articles across International and Iranian Journals

Reviewing literature reveals that identifying generic structure of research articles (RAs) in different fields of study, especially ESP, has received much attention. The major purpose behind such trends of research has been raising researchers' awareness of the common conventions in writing RAs. Along with this universal trend, a lot of genre studies have been done in Iranian context; however, it seems that ESP RAs have not been paid due attention yet. Hence this study aimed at investigating the generic structure of ESP RAs in international and Iranian journals. Applying Kanoksilapatham's (2007) model to the compiled corpus, it was found that contextualizing the study (Move 1) and consolidating results (Move 2) were the obligatory moves in Discussion section of ESP RAs across international and Iranian journals. Evaluation of the findings was a new step found in international Discussion sections but absent in Iranian ones. Related discussion of these findings prepares the researchers for publishing in international and Iranian ESP journals.Keywords: ESP, Discussion section, Move, Step, Author presence, Generic structur

QSAR Study of Anthranilic Acid Sulfonamides as Inhibitors of Methionine Aminopeptidase-2 using different chemometrics tools

Quantitative structure activity relationships (QSAR) studies, as one of the most important areas in chemometrics, play a fundamental role in predicting the biological activity of new compounds and identifying ligand-receptor interactions. Quantitative relationships between molecular structure and methionine aminopeptidase-2 inhibitory activity of a series of anthranilic acid sulfonamides derivatives were discovered by different chemometrics tools including factor analysis based multiple linear regressions (FA-MLR), principale component regression analysis (PCRA) and genetic algorithm-partial least squares GA-PLS. The FA-MLR describes the effect of geometrical and quantum indices on enzyme inhibition activity of the studied molecules. The quality of PCRA equation is better than those derived from FA-MLR. GA-PLS analysis indicated that the topological (IC4 and MPC06), constitutional (nf) and geometrical (G (N..S)) parameters were the most significant parameters on methionine aminopeptidase-2 inhibitory activity. A comparison between the different statistical methods employed revealed that GA-PLS represented superior results and it could explain and predict 85% and 77% of variances in the pIC50 data, respectively

Emergency Calls in the City of Vaughan (Canada) During the COVID-19 Pandemic: A Spatiotemporal Analysis

[EN] The COVID-19 pandemic has required governments to introduce various public health measures in order to contain and manage the pandemic’s unprecedented impacts in terms of illnesses and deaths. This study analyzes the spatiotemporal distribution of emergency incidents in Vaughan, a medium-sized city in the Canadian province of Ontario, comparing occurrences prior to and during the pandemic. Emergency calls received and responded to by the Vaughan Fire and Rescue Service were examined using spatial density and emerging hotspot analysis based on 11 periods of various public health measures and restrictions set in place from 17 March 2020 to 15 July 2021, as compared with corresponding pre-pandemic periods in the preceding three years (2017-2019). The resulting analyses show significant spatiotemporal changes in emergency incident patterns, particularly during periods of more stringent public health measures such as ‘stay at home’ orders or lockdowns of nonessential business establishments. Results of the study could provide useful insights for managing emergency service resources and operations during public health emergencies.This research has been conducted with financial support from the Social Sciences and Humanities Research Council of Canada (SSHRC) as part of its Partnership Engage Grants (PEG) COVID-19 Special Initiative. The Vaughan Fire and Rescue Service is the partner organization of the York University research team in this effort. The research work has been also supported by ADERSIM, funded by Ontario Research Fund (ORF).Asgary, A.; Solis, A.; Khan, N.; Wimaladasa, J.; Sabet, M. (2022). Emergency Calls in the City of Vaughan (Canada) During the COVID-19 Pandemic: A Spatiotemporal Analysis. En 4th International Conference on Advanced Research Methods and Analytics (CARMA 2022). Editorial Universitat Politècnica de València. 197-204. https://doi.org/10.4995/CARMA2022.2022.1508719720

Garra roseae, a new species from the Makran region in southern Iran (Teleostei: Cyprinidae)

Garra roseae, new species, is described from the stream Tang-e-Sarhe in the Iranian Makran region. It is distinguished from its congeners in the Middle East by lacking barbels, having a small mental disc, 42-58 total scales along the lateral line, 24-30 scales along the predorsal midline, and 20-24 circumpeduncular scales. It is further characterised by having five diagnostic nucleotide substitutions and a minimum K2P distance of 5.39% to G. rossica and 5.49% to G. nudiventris in the mtDNA COI barcode region. Garra phryne from eastern Iran is considered to be a synonym of G. nudiventris.We are pleased to thank the University of Guilan for financial support

Effect of low-temperature precipitates on microstructure and pseudoelasticity of an Fe–Mn–Si-based shape memory alloy

Fe–Mn–Si-based shape memory alloys (Fe-SMAs) have attracted much research attention due to their potential applications for vibration mitigation, energy dissipation, and re-centering in the construction sector. Because of the crucial impact of precipitation on the pseudoelasticity (PE) behavior of Fe-SMAs, the equilibrium phase diagram of an Fe–17Mn–5Si–10Cr–4Ni–1(V-C) (wt%) SMA was used in this study to identify a low-temperature precipitate and study its effect on the microstructure and PE of the alloy after a low-temperature aging process. Transmission electron microscopy (TEM) studies revealed that aging at 485 °C for 6 h after aging at 750 °C for 6 h led to the precipitation of fresh, parallelogram-shaped, (Cr–V–C)-rich precipitates along with elliptical-shaped, V-rich precipitates in the austenite grains of the recrystallized samples. Numerous parallel stacking faults (SFs) were formed due to the presence of the precipitates within the austenite grains. It is postulated that such an arrangement of SFs can further improve the PE by reducing the activation energy for the nucleation of ɛ-martensite laths and inhibiting them from colliding with each other and consequent formation of α'-martensite, resulting in a residual strain reduction to 2.7% after 4.0% tensile straining

Developmental morphology and growth patterns of laboratory-reared giraffe cichlid, Nimbochromis venustus Boulenger, 1908

The giraffe cichlid Nimbochromis venustus Boulenger, 1908 is a well-known species in aquarium trade. The laboratory-reared electric giraffe cichlid was studied in terms of early morphological development and allometric growth pattern from hatching until the juvenile stage i.e.51 dph. Total length increased linearly from hatching until 51 dph, with a high regression coefficient. The yolk sac exhaustion completed throughout 15 days. Significant morphological and morphometric variations occurred before the yolk sac absorption and early juvenile stage by evolving the anterior and posterior body section which improve swimming ability and food capturing. Positive growth coefficient of the body and trunk lengths were occurred after inflexion point coinciding with development of digestive system function. Eye diameter showed negative allometry at the inflexion point of 9.3 mm, continuing with positive growth rate until day 51. Body shape variation in early life history revealed consequent development in anterior and posterior body section in preflexion phase and development of body and trunk length in post flexion phase to support high survival of larvae. The present study emphasizes ontogenic study of different species to interpret biology and ecology of fish in association with evolutionary biology

Factors Affecting Academic Failure In Region II Medical Universities Students

Academic failure is an important issue in medical students which, if not controlled, will cause a drop in the academic level of physicians and paramedics in the coming years. Therefore, identification of factors affecting academic failure can influence the future planning of universities and reduce those factors and help effectiveness and flourishing of these students. This study aimed to investigate the factors influencing academic failure in medical universities' students in the region II of country Which and its results can be applicable to Whole country. This study is a case-control study including all students with academic failure in the academic year 2009 in Semnan, Mashhad, Gorgan, Shahroud, Sabzevar, Gonabad and Bojnoord Universities of Medical Sciences (Probation, increased educational semesters, failure in comprehensive medical tests).Data on students with academic failure and other students was collected and analysed using SPSS software by chi-square test at 5% significance level. The prevalence of academic failure in men was higher than women (N=571 , 5.33%). There was a significant difference between two groups in terms of gender, type of university, degree and entry quota (P &lt;0.05). Given the significant differences in some demographic and educational characteristics between students with and without academic failure, special attention and planning in this area are necessary

Body shape variation of Garra rufa (Teleostei, Cyprinidae) populations in the Tigris basin in Iran using geometric morphometric analysis

Geometric morphometric method was used to examine the body shape variations among the six populations of Garra rufa, in Iranian part of Tigris basin. A total of 15 landmark-points was used for 170 specimens to hypothesize population differentiation of G. rufa in the six rivers and reservoir. In discriminant function analysis, 85.9% of original grouped cases correctly classified. Principal component analysis (PCA) and canonical variates analysis (CVA) confirmed the significant difference between the populations. The results revealed that the studied populations are divided into three clades based on differences in body depth, caudal peduncle length, backward moving of anal fin. Caudal peduncle showed shortening trend in five populations. Narrower body shape was dominated among specimens of four regions. Studies on body shape provide supporting data on fisheries, stock management, and conservative programs

Promjene u RF-amidu srodnom peptidu-3 hipotalamusa i ekspresijama gena Kiss1 tijekom spermatogeneze kod štakora u uvjetima kroničnog stresa.

The effects were evaluated of chronic stress and the glucocorticoid receptor antagonist (RU486) on mRNA expressions of RF-amide related peptide-3 (RFRP-3) in the dorsomedial hypothalamic nucleus (DMH) and Kiss1 in the arcuate nucleus (ARC) of male rats. Twenty-four male rats were allocated to four equal sized groups: the stress, RU486, stress/RU486, and control groups. In the stress group the rats were restrained 1 hour/day for 12 days. In the RU486 group, the rats were injected with RU486 for 12 days. In the stress/RU486 group, the rats were injected with RU486 1 hour before the stress process for 12 days. Relative expressions of RFRP-3 and Kiss1 mRNAs were determined using real-time PCR. The relative expression of RFRP-3 mRNA in the stress group was higher than that in the RU486 and control rats. The relative expression of RFRP-3 mRNA did not differ between the stress group and the stress/RU486 rats. Furthermore, the relative expressions of Kiss1 mRNA in the stress, RU486, and stress/RU486 groups were less than that of the control rats. The relative expression of Kiss1 mRNA did not differ between the stress, RU486, and stress/RU486 groups. In conclusion, dysfunction in male rat fertility caused by the chronic stress may be the result of the increase in REFP-3 and the decrease in Kiss1 mRNA expression.Istražen je učinak kroničnog stresa i antagonista glukokortikoidnog receptora (RU486) na ekspresiju mRNA RF-amidu srodnog peptida-3 (RFRP-3) u dorzomedijalnoj jezgri hipotalamusa (DMH), te na ekspresije gena Kiss1 u arkuatnom nukleusu (ARC) štakora. Dvadeset i četiri štakora bila su raspodijeljena u četiri jednake skupine: stresna skupina, RU486 skupina, stresna/RU486 skupina i kontrolna skupina. U stresnoj skupini štakori su 12 dana bili obuzdani tijekom jednog sata dnevno. U skupini RU486, štakorima je tijekom 12 dana bio primijenjivan RU486. U skupini stres/RU486, štakorima je tijekom 12 dana apliciran RU486 jedan sat prije postupka obuzdavanja. Relativne ekspresije RFRP-3 i Kiss1 mRNA određene su lančanom reakcijom polimerazom u stvarnom vremenu. Relativna ekspresija RFRP-3 mRNA u stresnoj skupini bila je veća nego u skupini RU486 i kontrolnoj skupini. Relativna ekspresija RFRP-3 mRNA nije bila različita između stresne skupine i stres/RU486 skupine. Nadalje, relativne ekspresije Kiss1 mRNA u stresnoj skupini, skupini RU486, i stresnoj skupini/RU486 bile su manje u odnosu na kontrolnu skupinu. Relativna ekspresija Kiss1 mRNA nije se razlikovala između stresne skupine, skupineRU486 i stresne skupine/RU486. Zaključno, disfunkcija plodnosti kod štakora izloženih kroničnom stresu može biti uzrokovana putem povećane ekspresije RFRP-3 i smanjene ekspresije Kiss1 mRNA