Larvicide and insecticide activity of the essential oil of Ocimum sanctum var. cubensis in the control of Musca domestica (Linnaeus, 1758), under laboratory conditions

Essential oils have been considered as an important alternative facing synthetic pesticides. The control of the species Musca domestica is very important for public health due to their role as vector associated with Vibrio cholerae, Salmonella and Shigella, among other pathogens. The objective of this work was to evaluate the larvicidal and insecticidal efficacy of the essential oil of Ocimum sanctum var. cubensis, in the control of Musca domestica and its effect on the post-embryonic development of this species. The essential oil was applied topically in six concentrations to newly hatched larvae (1μL / larva). Mortality, interval length of each development period, as well as the weight of mature larvae were analyzed. An insecticidal bioassay was also carried out in adult flies. The essential oil of O. sanctum caused a slight reduction in the larval and pupal period for some of the concentrations evaluated. The time of development of the newly hatched larvae until the adult period was shortened in all the concentrations analyzed. The larvae of the control group were the heaviest. The effectiveness of the product in the control of the larvae of the flies of the species M. domestica was greater than 50%, except for those larvae treated with 80.25 mg/mL of the essential oil. The lethal concentration for adult insects LC50 was estimated at 9.41μg. Results demonstrated that the essential oil of O. sanctum has effect in all stages of the life cycle of M. domestica, extolling its potential as an effective insecticide against this species.&nbsp

Insecticidal activity of the essential oil of Ocimum sanctum var. cubensis in the control of Cochliomyia macellaria (Fabricius), under laboratory conditions

El uso de insecticidas químicos sintéticos para el control de insectos es peligroso, afectan- do al hombre y los animales, contaminando el aire, el agua, el suelo y la cadena alimentaria, ocasionando graves efectos sobre la salud y propiciando el desarrollo de resistencia en varias especies de plagas y vectores. Todos estos problemas incrementan el interés por el desarrollo de insecticidas alternativos de menor impacto ambiental. Esta investigación se trazó como objetivo determinar el efecto insecticida del aceite esencial de Ocimum sanctum var. cubensis sobre la especie de moscas Cochliomyia macellaria (Fabricius). El aceite esencial fue extraído de las partes aéreas de la planta por el método de hidro-destilación en un equipo Clevenger. La colonia de dípteros muscoides fue mantenida según el procedimiento normalizado del Laboratorio de Entomología Médica y Forense del Instituto Oswaldo Cruz - LEMEF / FIOCRUZ. Para evaluar los efectos del aceite esencial sobre el desarrollo post-embrionario de las moscas, se ensayaron seis concentraciones (5, 10, 25, 50, 75 y 100%) en aplicación tópica 1μL/larva. Se obtuvo como resultado que en todos los tratamientos las larvas mostraron valores de masa corporal menores al compararlos con los grupos controles. El análisis de la duración de las etapas larval y pupal, así como del período de neolarva-adulto evidenció hubo un comportamiento que establece una diferencia entre los grupos tratados y los grupos controles. La utilización de este aceite esencial no influyó en la variable biológica razón sexual. Se concluye que el aceite esencial de Ocimum sanctum var. cubensis mostró actividad insecticida contra la especie Cochliomyia macellaria afectando los parámetros masa corporal y duración del período de neolarva-adulto, causando además mortalidad en todas las concentraciones probadas, por lo puede considerarse como un insecticida botánico de menor impacto ambiental para el control alternativo de esta especie de moscasO uso de inseticidas químicos sintéticos para o controle de insetos é perigoso, afetando o homem e os animais, contaminando o ar, a água, o solo e a cadeia alimentar, causando sérios efeitos à saúde e promovendo o desenvolvimento de resistência em várias espécies de pragas e vetores. Todos esses problemas aumentam o interesse no desenvolvimento de inseticidas alternativos com menor impacto ambiental. O objetivo desta investigação foi determinar o efeito inseticida do óleo essencial de Ocimum sanctum var. cubensis sobre as espécies de moscas Cochliomyia macellaria (Fabricius). O óleo essencial foi extraído das partes aéreas da planta pelo método de hidrodestilação em um equipamento de Clevenger. A colônia dípteros muscoides foi mantida de acordo com o procedimento padrão do Laboratório de Entomologia Médica e Forense do Instituto Oswaldo Cruz - LEMEF / FIOCRUZ. Para avaliar os efeitos do óleo essencial no desenvolvimento pós-embrionário das moscas, seis concentrações (5, 10, 25, 50, 75 e 100%) aplicado topicamente 1 ul / larva testado. O resultado foi que em todos os tratamentos as larvas apresentaram menores valores de massa corporal quando comparados com os grupos controle. A análise da duração dos estágios larval e pupal, bem como do período neolarva-adulto, mostrou um comportamento que estabelece uma diferença entre os grupos tratados e os grupos controle. O uso deste óleo essencial não influenciou a variável biológica razão sexual. Conclui-se que o óleo essencial de Ocimum sanctum var. cubensis mostrou actividade insecticida contra Cochliomyia macellaria espécies que afecta os parâmetros de massa corporal e duração da neolarva-adulto, e fazendo com que a mortalidade em todas as concentrações testadas, assim pode ser considerado um insecticida botânico menor impacto ambiental para o controlo alternativo do presente espécies de moscas.The use of synthetic chemical insecticides for the control of insects is dangerous, affecting man and animals, contaminating the air, water, soil and the food chain, causing serious effects on health and promoting the development of resistance in several species of pests and vectors. All these problems increase the interest in the development of alternative insecticides with lower environmental impact. The objective of this investigation was to determine the insecticidal effect of the essential oil of Ocimum sanctum var. cubensis on the species of flies Cochliomyia macellaria (Fabricius). The essential oil was extracted from the aerial parts of the plant by the method of hydro-distillation in a Clevenger equipment. The colony of muscoid diptera was maintained according to the standard procedure of the Medical Entomology and Forensic Laboratory of the Oswaldo Cruz Institute - LEMEF / FIOCRUZ. To evaluate the effects of the essential oil on the post-embryonic development of the flies, six concentrations (5, 10, 25, 50, 75 and 100%) were tested in topical application 1μL / larva. The result was that in all the treatments the larvae showed lower body mass values when compared with the control groups. The analysis of the duration of the larval and pupal stages, as well as the period of neolarva-adult showed a behavior that establishes a difference between the treated groups and the control groups. The use of this essential oil did not influence the biological variable sexual reason. It is concluded that the essential oil of Ocimum sanctum var. cubensis showed insecticidal activity against the species Cochliomyia macellaria, affecting body mass parameters and duration of the neolarva-adult period, causing mortality at all tested concentrations, so it can be considered as a botanical insecticide with lower environmental impact for the alternative control of this species of flies

‘People lie’: overcoming obstacles to incorporate social science research to biodiversity conservation

Mesmo com o reconhecimento da importância da interdisciplinaridade na conservação da biodiversidade, ainda há resistência em incorporar a pesquisa em ciências sociais (PCS) ao pensamento e à prática conservacionista. As razões para tal resistência podem ser resumidas em três afirmações gerais ainda comumente atribuídas à PCS: 'tem pouca utilidade' e 'menos rigor metodológico' quando comparada à pesquisa em ciências naturais e, sobretudo, é pouco confiável porque 'as pessoas mentem'. Neste ensaio, desenvolvido a partir da experiência dos participantes de uma comunidade de prática, formada por profissionais de diversas áreas e setores relacionados à conservação, e das discussões geradas nesse espaço de aprendizado coletivo, abordamos as limitações e os equívocos por trás das afirmações acima. A PCS não é menos útil na conservação e nem tem menos rigor metodológico do que a pesquisa em ciências naturais, e quando as pessoas mentem para o pesquisador o problema não está na pesquisa em si, mas na relação entre sujeito e pesquisador. Argumentamos que à medida que os conservacionistas se familiarizam com a PCS e que os princípios de equidade e justiça são incorporados aos valores e objetivos da conservação, a importância e necessidade da PCS na conservação tornam-se óbvias, e a falta de confiança entre pesquisador e sujeitos deixa de ser uma preocupação significativa. Capacitar, integrar e apoiar são nossas recomendações básicas para pesquisadores, educadores, gestores e tomadores de decisão nas áreas de conservação, ensino, publicação e financiamento, para que a PCS cumpra plenamente seu papel na conservação.Despite the acknowledged importance of interdisciplinarity in biodiversity conservation, there is still resistance to incorporate social science research (SSR) to both conservationist thinking and practice. The reasons for such a resistance can be summarized in three general statements still commonly attributed to SSR, namely: it is of 'little use' and it has 'less methodological rigor' than research in the natural sciences and, above all, it is unreliable because 'people lie'. The current essay was developed based on the experience of participants of a community of practice (formed by professionals from different fields and sectors  associated with conservation), as well as on discussions held in this space of collective learning. It addresses the limitations and misconceptions behind the aforementioned statements. SSR is not less useful in conservation and not less methodologically rigorous than research conducted in the natural sciences. When researchers are lied to, the problem does not lie on the research itself, but on the subject-researcher relationship. We herein argue that as conservationists become more familiar with SSR, and as principles like equity and justice are incorporated to conservation values and goals, both the importance and need of SSR in conservation become obvious, making the lack of trust between researcher and subjects no longer a significant concern. Increasing capacity, integrating and supporting are our basic recommendations for researchers, educators, managers and decision-makers in the conservation, teaching, publishing and funding fields, so that SSR can fully fulfill its role in conservation

Evaluation of Depression, Anxiety and Sleep Quality in the Brazilian Population During Social Isolation Due to the New Coronavirus (SARS-CoV-2) pandemic: the DEGAS-CoV Study/ Avaliação da Depressão, Ansiedade e Qualidade do Sono na População Brasileira Durante o Isolamento Social Devido à Nova Pandemia do Coronavírus (SARS-CoV-2): o Estudo DEGAS-CoV

Introduction: The new coronavirus infection (COVID-19) has caused distress and repercussions in mental and physical health of individuals. Depression, anxiety and worsening of sleep quality have been reported in several recent articles that surveyed populations all over the globe. Our work meant to access, through a cross-sectional study, these disorders in the Brazilian population, through the application of an online questionnaire conducted on the second trimester of 2020. Materials and Methods: We applied an online questionnaire, filled with questions regarding social, economic, financial, educational and health status, as well as questions from the Hospital Anxiety and Depression Scale (HAD), and from the Pittsburgh Sleep Quality Index (PSQI).Results: We collected 2,695 valid answers, from April 24th to May 31st, 2020. Age ranged from 18 to 79 years, mean of 31.3. Women were 76.3%, men 23.7%. Symptoms of Anxiety were found in 56.5%, of depression in 46.1%, and of bad sleep in 49.2%. Some groups were more prone than others to one or more of those conditions, such as: younger people, women, mestizos, people with lesser years of education, of lower income or whose income dropped significantly during the pandemic, caregivers, students, sedentary or people practicing less physical activity, people who followed more hours of news of COVID-19 and those less engaged in social and instrumental activities.Conclusion: anxiety, depression and bad sleep quality were significantly high in our survey. Mental and sleep health is heterogeneously affected among individuals, depending on social, economic, financial, educational and health status

Identificação de imaturos de dípteros muscóides (Diptera: Calliphoridae) de importância sanitária e forense através da microscopia eletrônica de varredura

Submitted by Anderson Silva ([email protected]) on 2012-07-18T19:14:53Z No. of bitstreams: 1 paloma_m_mendonca_ioc_bp_0035_2010.pdf: 5136216 bytes, checksum: 96c991db74df0f84ca5c4542f83fd6f4 (MD5)Made available in DSpace on 2012-07-18T19:14:53Z (GMT). No. of bitstreams: 1 paloma_m_mendonca_ioc_bp_0035_2010.pdf: 5136216 bytes, checksum: 96c991db74df0f84ca5c4542f83fd6f4 (MD5) Previous issue date: 2010CNPq e FAPERJFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Os dípteros muscóides são insetos com notável importância econômica e na entomologia médica e veterinária devido às diversas formas como eles podem estar relacionados ao homem, seja vetorando patógenos, causando miíases ou participando da degradação da matéria orgânica. A entomologia forense é uma ciência que estuda o papel dos insetos na decomposição de cadáveres e uma das principais aplicações destes nesta área é a estimativa do intervalo pós-morte, baseada no período mínimo de atividade dos insetos no corpo. No caso dos dípteros muscóides, que são os primeiros a chegar e podem ovipor logo após encontrar o cadáver, o conhecimento morfológico das fases imaturas desses insetos será mais uma ferramenta que auxiliará peritos e pesquisadores, pois em alguns casos, ovos e larvas são as únicas evidências presentes. A microscopia eletrônica de varredura é um instrumento que permite uma visualização mais detalhada dos caracteres morfológicos. Algumas espécies de muscóides destacam-se devido a sua preferência alimentar, pois buscam a matéria orgânica em decomposição para se alimentar, dentre elas podemos destacar os califorídeos Chrysomy albiceps, Chrysomya putoria e Chrysomya megacephala. Este trabalho teve como objetivo a identificação de ovos, larvas de primeiro, segundo e terceiro instar e pupários destas espécies de dípteros de grande importância forense e disseminadores de patógenos, através da utilização de microscopia eletrônica de varredura. Foram analisados 30 exemplares de cada estágio correspondente a cada espécie, provenientes de colônias de laboratório, estabelecidas a partir de adultos capturados no campus da Fundação Oswaldo Cruz, RJ. Ovos e larvas foram transferidos para placas de Petri contendo hidróxido de sódio e lavados com H 2O destilada. Depois foram fixados com solução de glutaraldeído em tampão cacodilato de sódio e pós-fixados em tetróxido de ósmio, desidratados em séries crescentes de acetona e submetidos ao método de secagem pelo ponto crítico usando CO2 em aparelho de Balzers, montados em suportes metálicos e cobertos por ouro, para permitir a visualização ao microscópio eletrônico de varredura. Pupas com 48 a 72 horas de formação foram lavadas, congeladas e depois montadas em suportes metálicos e cobertas por ouro para observação. Neste trabalho foi observado que a linha mediana termina arredondada na porção anterior dos ovos de C. putoria, enquanto nas outras duas espécies esta termina bifurcada. As ornamentações da micrópila são do tipo projeções nos ovos de C. megacephala e C. albiceps e do tipo depressões em C. putoria. O corpo da larva em todos os instares é muito semelhante entre as três espécies analisadas. No entanto, somente C. albiceps apresentou o tegumento recoberto por tubérculos organizados em fileiras ao longo do corpo da larva. No ápice dos tubérculos são encontrados espinhos que são característicos dessa espécie. Os pupários são formados a partir do endurecimento da cutícula da larva de terceiro instar, sendo assim, apresentaram estruturas muito semelhantes ao instar anterior. Este estudo permitiu destacar diversas características que podem ser utilizadas para identificar as espécies de importância sanitária e forense, além de fornecer subsídios para a elaboração de uma chave dicotômica específica para estes imaturos.Muscoids diptera are insects with considerable economic importance and also in medical and veterinary entomology because of the way that they are related to man, as vectors of pathogens, causing myiasis or participating in organic matter degradation. Forensic entomology is a discipline that studies the role of insects in corpse’s decomposition and one of the most commom applications is to estimate postmortem interval by determining a minimum period of insect activity on a decomposing body. Flies are the first visitors to a dead body and are able to oviposit on carrion within a few hours after death. And the morphologic knowledge from imatures can be used as a tool in crime scene investigations because, sometimes, only eggs and larvae can be found in corpses. Scanning electron microscopy gives detailed information about morphologic characters. Some species of diptera are important because their larvae grow up at organic matter, such the blowflies Chrysomya albiceps, Chrysomya putoria and Chrysomya megacephala. The aim of this study was identify eggs, larvae of first, second and third instar and puparium of these species of flies of forensic importance and pathogens vectors, by using scanning electron microscopy. All the imatures examined in this study were obtained from culture set up from wild flies trapped at Fundação Oswaldo Cruz campus, Rio de Janeiro, RJ. Thirty samples from each species were observed. Eggs and larvae were transferred to a Petri dish with sodium hydroxid and then washed with destilated water. After it, imatures were fixed in glutaraldehyde in sodium cacodylate and postfixed in osmium tetroxide. They were dehydrated in a series of acetone for critical point drying, using CO 2 in Balzers apparatus. Specimens were adhered in metalic support and gold-coated for SEM examination. Pupae within 48 to 72 hours-age were washed, frozen and adhered in metalic support and gold- coated for SEM examination. Median area ends rounded anteriorly in C. putoria, whereas ended bifurcate in others species. Micropyle showed projections at C. megecephala and C. albiceps, but at C. putoria, micropyle was adorned by depressions. Larvae bodies were very similar among all the species analyzed. However, only C. albiceps body tegument was composed by tubercles organized in lines along larval body. At the apex of these tubercles some spines are only seen at this blowfly specie. The pupae are formed from the hardening of the cuticle of third instar larvae, thus, had structures very similar to the previous instar. This study helped to highlight many features that could be used to identify the species of sanitary and forensic importance, and provides subsidies for the development of a key to these specific immature

Rearing Chrysomya megacephala on artificial diets composed of varying concentrations of albumin

Larvae of the blowfly Chrysomya megacephala were reared on an artificial diet composed of five different concentrations of albumin (2, 4, 6, 8 and 10%) and the control group was fed on putrid bovine meat. No larvae developed in the 2 and 10% albumin concentrations. The period from newly hatched larvae to adults reared on 4, 6 and 8% albumin was 13.1, 13.1 and 13.6 days, respectively, whereas for the control group, it was 11.2 days. Concentrations of 4, 6 and 8% albumin proved viabile for larval periods of 29.3, 44.0 and 57.3%, respectively, whereas for the control group, it was 77.3%. Pupal viability was 77.3, 36.4 and 83.7%, while for the control group, it was 84.5%; the newly hatched larvae to adult viability was 21.3, 16.0 and 48.0%, respectively, and for the control group, it was 65.3%.O presente trabalho teve como objetivo avaliar o desenvolvimento pós-embrionário de Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) em dietas artificiais à base de albumina nas concentrações de 2%, 4%, 6%, 8% e 10% de albumina e como controle foi utilizada a carne bovina moída putrefata. Não houve desenvolvimento nas dietas de concentração 2% e 10% de albumina. A duração do período de larva a adulto foi 13,1; 13,1 e 13,6 dias, respectivamente, e a do controle foi de 11,2 dias. As dietas artificiais nas concentrações 4, 6 e 8% de albumina apresentaram viabilidades larvais de 29,3; 44,0 e 57,3%, respectivamente e o controle 77,3%; as viabilidades de pupa foram 77,3; 36,4 e 83,7% e o controle 84,5%, respectivamente; as viabilidades de larva a adulto foram 21,3; 16,0 e 48,0% e o controle foi de 65,3%, respectivamente

Ultrastructure of immature stages of Cochliomyia macellaria (Diptera: Calliphoridae), a fly of medical and veterinary importance

Made available in DSpace on 2015-06-10T13:55:21Z (GMT). No. of bitstreams: 2 license.txt: 1914 bytes, checksum: 7d48279ffeed55da8dfe2f8e81f3b81f (MD5) lucas_cortinhasetal_IOC_2014.pdf: 8484428 bytes, checksum: b02f39c989dddecc3d43aeaa71c76d40 (MD5) Previous issue date: 2014Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmaniose. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Programa de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmaniose. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Programa de Pós-Graduação em Biologia Animal. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmaniose. Setor de Entomologia Médica e Forense. Programa de Pós-Graduação em Biodiversidade e Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmaniose. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmaniose. Setor de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Cochliomyia macellaria (Diptera: Calliphoridae) is known as the secondary screwworm because it causes secondary or facultative myiasis when the larvae feed on necrotic tissues. This fly has a significant medical and veterinary importance since it has been reported to transport eggs of Dermatobia hominis (human botfly), which can cause significant economic losses to livestock. Since this screwworm has been collected colonizing both pig carcasses and human cadavers, it is considered one of the most important species for forensic entomology studies. Scanning electron microscopy (SEM) gives detailed information on the morphological characteristics which can help identify the immature forms of the flies. The aim of this study was to describe and analyze the morphological characteristics of the eggs, all the larval instars, and the puparia of Cochliomyia macellaria using SEM. The egg is ellipsoid and the dorsal surface is concave. The islands inside the median area had no anastomosis, but some perforations could be observed. From the second larval instar onwards, besides the intersegmental spines, other bands of spines were observed at the abdominal segments. Two spiracular openings were visible on the first and second larval instars, which were not expected. These characteristics are specific to Cochliomyia genus. The number and the general aspect of the spine tips in the cephalic region, the intersegmental bands on the abdomen, and the number of the spiracular openings could together help identify C. macellaria

First record of Leptus sp. Latreille, 1796 (Acari: Erythraeidae) from Itaipuaçu beach, Maricá, RJ, Brazil

Made available in DSpace on 2015-05-15T13:16:43Z (GMT). No. of bitstreams: 2 license.txt: 1914 bytes, checksum: 7d48279ffeed55da8dfe2f8e81f3b81f (MD5) zeneida_pinto2etal_IOC-2014.pdf: 1565801 bytes, checksum: 0963217cf5ffab4145e7e0bb43dc8d83 (MD5) Previous issue date: 2014Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Educação em Ambiente e Saúde. Rio de Janeiro, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Programa de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Programa de Pós-Graduação em Biologia Animal (PPGBA). Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biodiversidade e Saúde. Laboratório de Referência Nacional em Vetores das Riquetsioses (LIRN). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Programa de Pós-Graduação em Biologia Animal (PPGBA). Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Programa de Pós-Graduação em Ciências Veterinárias. Seropédica, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ, Brasil.First record of larvae from genus Leptus (Acari: Erythraeidae) in Itaipuaçu beach, Rio de Janeiro, Brazil

Insecticidal effects of Ocimum sanctum var. cubensis essential oil on the diseases vector Chrysomya putoria

Context: The blowfly Chrysomya putoria is widely distributed throughout the Neotropical region and, besides transmitting pathogens; they could cause secondary myiasis. Botanical insecticides provide an alternative to synthetic pesticides because the excessive use of synthetic insecticides resulted in a progressive resistance of the pests to these chemicals, diminishing their effectiveness and generating consequences with negative environmental impact. The essential oil extracted from Ocimum sanctum (basil) has showed insecticidal activity against some insects but has no reported studies on the activity of this plant against flies. Aims: To evaluate the insecticidal effects of Ocimum sanctum var. cubensis Gomes essential oil on the post embryonic development of Chrysomya putoria. Methods: The colonies of Chrysomya putoria were established and maintained at the Laboratório de Entomologia Médica e Forense (FIOCRUZ), Rio de Janeiro, Brazil. The basil essential oil was tested in six concentrations (4.13, 8.25, 20.63, 41.25, 61.87 and 80,25 mg/mL). Mortality and changes in life cycle were recorded daily. Results: β-caryophyllene, β-selinene and eugenol, were the main constituents of the basil essential oil. The experiments demonstrated that in all concentrations tested, this essential oil shortening the duration of all post embryonic stages having a direct impact in the viability of this fly estimating the LC50 in 7.47 mg/mL of concentration. In addition, the essential oil caused morphological alterations in abdomen, wings and ptilinum at lower concentrations. Conclusions: This essential oil emerge as a good option for the control of the disease vector blowfly Chrysomya putoria