Inheritance of genomic regions and genes associated with number of oocytes and embryos in Gir cattle through daughter design.

Over the past decades, daughter designs, including genotyped sires and their genotyped daughters, have been used as an approach to identify QTL related to economic traits. The aim of this study was to identify genomic regions inherited by Gir sire families and genes associated with number of viable oocytes (VO), total number of oocytes (TO), and number of embryos (EMBR) based on a daughter design approach. In total, 15 Gir sire families were selected. The number of daughters per family ranged from 26 to 395, which were genotyped with different SNP panels and imputed to the Illumina BovineHD BeadChip (777K) and had phenotypes for oocyte and embryo production. Daughters had phenotypic data for VO, TO, and EMBR. The search for QTL was performed through GWAS based on GBLUP. The QTL were found for each trait among and within families based on the top 10 genomic windows with the greatest genetic variance. For EMBR, genomic windows identified among families were located on BTA4, BTA5, BTA6, BTA7, BTA8, BTA13, BTA16, and BTA17, and they were most frequent on BTA7 within families. For VO, genomic windows were located on BTA2, BTA4, BTA5, BTA7, BTA17, BTA21, BTA22, BTA23, and BTA27 among families, being most frequent on BTA8 within families. For TO, the top 10 genomic windows were identified on BTA2, BTA4, BTA5, BTA7, BTA17, BTA21, BTA22, BTA26, and BTA27, being most frequent on BTA7 and BTA8 within families. Considering all results, the greatest number of genomic windows was found on BTA7, where the VCAN, XRCC4, TRNAC-ACA, HAPLN1, and EDIL3 genes were identified in the common regions. In conclusion, 15 Gir sire families with 26 to 395 daughters per family with phenotypes for oocyte and embryo production helped to identify the inheritance of several genomic regions, especially on BTA7, where the EDIL3, HAPLN1, and VCAN candidate genes were associated with number of oocytes and embryos in Gir cattle families

Parent-of-origin effects for the number of oocytes and embryos in Gir cattle.

Imprinting is a phenomenon that alters the expression of genes according to the parental origin of their alleles. A quantitative form to evaluate the imprinting effect is known as parent-of-origin effect. Our aim with this work is to identify parent-of-origin effects that influence the number of oocytes and embryos in Gir dairy cattle. A dataset with 17,526 Ovum Pick Up observations from 1641 Gir donors was used to estimate parent-of-origin effects for the traits number of total oocytes (TO), number of viable oocytes (VO) and number of embryos (EM). To identify parent-of-origin effects, dam and sire gametic effects were included, individually or together, in an animal model for TO, VO and EM traits. For TO, inclusion of paternal origin effects in the model was significant (P < 0.05), and explained 6 % of the total phenotypic variance. For VO and EM no significant parent-of-origin effects were found for either parental line. In conclusion, paternal effects appear to influence the total oocyte production in the Gir cattle breed

Uso da tecnologia de eletroforese microfluídica "lab-on-a-chip" para análises das proteínas do leite em fraudes de leite caprino com leite bovino.

[The use of lab-on-a-chip microfluidic electrophoresis technology for milk protein analysis from caprineibovine milk mixtures adulterations].Edição dos Anais do V Congresso Brasileiro de Qualidade do Leite do Conselho Brasileiro de Qualidade do Leite - CBQL, Águas de Lindóia, jun. 2013

The Combination of Gefitinib With ATRA and ATO Induces Myeloid Differentiation in Acute Promyelocytic Leukemia Resistant Cells

In approximately 15% of patients with acute myeloid leukemia (AML), total and phosphorylated EGFR proteins have been reported to be increased compared to healthy CD34+ samples. however, it is unclear if this subset of patients would benefit from EGFR signaling pharmacological inhibition. pre-clinical studies on AML cells provided evidence on the pro-differentiation benefits of EGFR inhibitors when combined with ATRA or ATO in vitro. despite the success of ATRA and ATO in the treatment of patients with acute promyelocytic leukemia (APL), therapy-associated resistance is observed in 5-10% of the cases, pointing to a clear need for new therapeutic strategies for those patients. In this context, the functional role of EGFR tyrosine-kinase inhibitors has never been evaluated in APL. here, we investigated the EGFR pathway in primary samples along with functional in vitro and in vivo studies using several APL models. we observed that total and phosphorylated EGFR (Tyr992) was expressed in 28% and 19% of blast cells from APL patients, respectively, but not in healthy CD34+ samples. Interestingly, the expression of the EGF was lower in APL plasma samples than in healthy controls. the EGFR ligand AREG was detected in 29% of APL patients at diagnosis, but not in control samples. In vitro, treatment with the EGFR inhibitor gefitinib (ZD1839) reduced cell proliferation and survival of NB4 (ATRA-sensitive) and NB4-R2 (ATRA-resistant) cells. moreover, the combination of gefitinib with ATRA and ATO promoted myeloid cell differentiation in ATRA- and ATO-resistant APL cells. In vivo, the combination of gefitinib and ATRA prolonged survival compared to gefitinib- or vehicle-treated leukemic mice in a syngeneic transplantation model, while the gain in survival did not reach statistical difference compared to treatment with ATRA alone. our results suggest that gefitinib is a potential adjuvant agent that can mitigate ATRA and ATO resistance in APL cells. therefore, our data indicate that repurposing FDA-approved tyrosine-kinase inhibitors could provide new perspectives into combination therapy to overcome drug resistance in APL patients

Estimativas de correlações entre os métodos, número de ovos por grama de fezes (OPG) e Famacha utilizados na identificação de helmintos em ovelhas Santa Inês e Morada Nova.

Objetivou-se com este estudo avaliar as estimativas de correlações entre indicadores do índice de verminose (OPG) e FAMACHA com informações de ovinos das raças Santa Inês e Morada Nova

Análise eletroforética microluídica "Lab-on-a-chip" das proteinas do leite para detectar adulteração de leite bovino adicionado com soro.

[Lab-on-a-chip microfluidic electrophoretic analisys of milk proteins to detect additional whey in bovine milk adulteration].Edição dos Anais do V Congresso Brasileiro de Qualidade do Leite do Conselho Brasileiro de Qualidade do Leite - CBQL, Águas de Lindóia, jun. 2013

Informações básicas sobre o município de Moju, Pará: uma contribuição para o seu planejamento.

bitstream/item/223661/1/Informacoes-basicas-sobre-o-municipio.pdfTrabalho financiado pelo Projeto GESPAN - Gestão Participativa de Recursos Naturais

Search for the standard model Higgs boson in the H to ZZ to 2l 2nu channel in pp collisions at sqrt(s) = 7 TeV

A search for the standard model Higgs boson in the H to ZZ to 2l 2nu decay channel, where l = e or mu, in pp collisions at a center-of-mass energy of 7 TeV is presented. The data were collected at the LHC, with the CMS detector, and correspond to an integrated luminosity of 4.6 inverse femtobarns. No significant excess is observed above the background expectation, and upper limits are set on the Higgs boson production cross section. The presence of the standard model Higgs boson with a mass in the 270-440 GeV range is excluded at 95% confidence level.Comment: Submitted to JHE