12 research outputs found
309 SEX SORTED BOAR SPERMATOZOA: TIME COURSE AND PROFILE OF THEIR IN VITRO PENETRATION ABILITY AFTER STORAGE IN THE PRESENCE OF HOMOLOGOUS SEMINAL PLASMA
19 LOW-DOSE DEEP INTRAUTERINE INSEMINATION IN SOWS UNDER CONDITIONS: INCIDENCE OF UNILATERAL FERTILIZATIONS
Vitrification of porcine embryos at various developmental stages using different ultra-rapid cooling procedures
International audienc
In vitro development following one-step dilution of OPS-vitrified porcine blastocysts
International audienc
Simple storage (CO2-free) of porcine morulae for up to three days maintains the in vitro viability and developmental competence
Three-to-5-day weaning-to-estrus intervals do not affect neither efficiency of collection nor in vitro developmental ability of in vivo-derived pig zygotes
Exogenous ascorbic acid enhances vitrification survival of porcine in vitro-developed blastocysts but fails to improve the in vitro embryo production outcomes
Non-viable sperm in the ejaculate: Lethal escorts for Contemporary viable sperm
Non-viable sperm ("dead sperm") are present invariable numbers in mammalian ejaculates and their number increase substantially when semen is stored, particularly cryopreserved. This review comparatively highlights, with experimental data in porcine, the role-played by non-viable sperm in the outcome of semen used in assisted reproductive technologies. As well, the review discusses our current understanding of their origin and the pathways involved when their large numbers negative influence the functional lifespan of contemporary viable sperm to eventually cause irreversible dysfunction that reduces their fertility potential and their ability to develop healthy embryos. Finally, it highlights procedures currently available to mitigate these harmful effects. (C) 2016 Elsevier B.V. All rights reserved.Funding Agencies|European Regional Development Fund; Spanish Government (MINECO-FEDER) [AGL2012-39903, MINECO AGL2015-69738-R]; Seneca Foundation of Murcia [19892/GERM/15]; Swedish Research Council VR, Stockholm, Sweden [2011-6353]; Swedish Research Council FORMAS, Stockholm, Sweden [2011-512]; Swedish Research Council FORSS, Stockholm, Sweden [312971]</p