Efecto de la defoliación post-quema sobre el crecimiento de dos gramíneas nativas en el Caldenal, Argentina

El crecimiento en los pastizales naturales semiáridos puede ser afectado por la longitud de tiempo que el pastoreo es excluido durante el período de regeneración post-fuego. El objetivo de este trabajo a campo fue estudiar el efecto de la defoliación post-fuego en diferentes estados fenológicos: vegetativo (defoliación temprana) y elongación de entrenudos (defoliación tardía) sobre el crecimiento de Piptochaetium napostaense y Poa ligularis, dos gramíneas nativas perennes muy importantes en la región semiárida templada central de Argentina (Caldenal). En general, los tratamientos de defoliación post-quema no afectaron el número de hojas verdes en las macollas de P. napostaense. La defoliación temprana después del tratamiento de fuego generalmente redujo el número de hojas verdes en las macollas de P. ligularis con respecto a las plantas control. Contrariamente, la defoliación tardía usualmente incrementó el número de hojas verdes en las macollas de P. ligularis. Sin embargo, en ambos casos, muy pocas de las diferencias detectadas fueron significativas (p0,05) a aquellas de las plantas no defoliadas. Inmediatamente después de los tratamientos de defoliación temprana y tardía, las tasas de crecimiento relativas de la altura y la longitud verde total de las macollas de las plantas de ambas especies fueron reducidas (p0.05) than values on non-defoliated plants, respectively. Immediately after early- and late-season defoliation treatments, relative growth rates for tiller height and total green length were reduced (p<0.05) on plants of both species with respect to control plants. Our results suggest that a one-year-period without severe defoliations after fire would be at least necessary for not risking the persistence of these perennial grasses in the Caldenal community.Fil: Pelaez, Daniel Valerio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Boo, Roberto Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Mayor, M. D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Elia, Omar Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Martinez, S. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentin

Obstructive Sleep Apnea Detection Methods Based on Heart Rate Variability Analysis: Opportunities for a Future Cinc Challenge

[EN] The effects of sleep-related disorders, such as obstructive sleep apnea (OSA), can be devastating either in children or adults. Misdiagnosis may lead to severe cardiovascular diseases. Besides, OSA consequences are often related to bad job performance, and road accidents. Nowadays, polysomnography (PSG) is still considered the gold standard for OSA diagnosis, but the required facilities are extremely high, thus reducing availability worldwide. For this reason, simpler and cost-effective diagnosing methods have been proposed in the late years. In this regard, the heart rate variability (HRV) has been demonstrated to strongly reflect apnea episodes during sleep. Hence, this work reviews the latest advances in the evaluation of OSA from the HRV perspective to consider its potentialities for a future revisited CinC Challenge.This research has been supported by grants DPI201783952-C3 from MINECO/AEI/FEDER EU, SBPLY/17/180501/000411 from Junta de Comunidades de Castilla-la Mancha and AICO/2019/036 from Generalitat Valenciana. Moreover, Daniele Padovano has held graduate research scholarships from Escuela Polit ' ecnica de Cuenca and Instituto de Tecnolog ' ias Audiovisuales, University of CastillaLa ManchaPadovano, D.; Martinez-Rodrigo, A.; Pastor, JM.; Rieta, JJ.; Alcaraz, R. (2020). Obstructive Sleep Apnea Detection Methods Based on Heart Rate Variability Analysis: Opportunities for a Future Cinc Challenge. IEEE. 1-4. https://doi.org/10.22489/CinC.2020.400S1

Challenges in the COVID-19 vaccination era: Prioritization of vaccines among essential workers in Mexico

The coronavirus disease-2019 (COVID-19) pandemic gave rise to a massive global health concern that has placed an unprecedented strain on healthcare systems, education and economy. The recent vaccine roll-out gave humanity a glimpse of hope. However, more than 50% of the vaccine supply has been acquired by high-income countries, forcing low- and middle-income countries to prioritize who should be vaccinated. In Mexico, the first phase of the vaccination program prioritized healthcare personnel working in front-line COVID-19 public institutions. The second phase was planned for the remaining healthcare workers attending at both COVID and non-COVID areas. The government, however, aiming to reopen schools, decided to vaccinate teachers instead. This raised several concerns, since Mexico tops the ranking of deaths among healthcare workers due to COVID-19 worldwide. Furthermore, the possible framing of vaccines as a political tool has caused commotion among the Mexican people and media, especially since 2021 is the Midterm Election year in Mexico, and the Teachers’ Union has historically played an essential role in this matter. Therefore, it is relevant to share the vaccination experience in resource-constrained settings to provide help and advice to reach an optimal strategy and deflate this pandemic

A pre-correlation RFI mitigation algorithm for L-band interferometric radiometers

Radio Frequency Interference (RFI) is a major concern for both real and synthetic aperture radiometers. After the lessons learnt from SMOS, ESA is preparing the next generation of L-band interferometric radiometers with RFI mitigation integrated into the cross-correlators. This work presents a preliminary design and results of a pre-correlation RFI mitigation algorithm tailored for interferometric radiometers. The results show that the correlation error introduced by the RFI is reduced on average to the half, with peaks of 20 dB of mitigation.Peer ReviewedPostprint (author's final draft

How are oxygen budgets influenced by dissolved iron and growth of oxygenic phototrophs in an iron-rich spring system? Initial results from the Espan Spring in Fürth, Germany

At present most knowledge on the impact of iron on 18O / 16O ratios (i.e. δ18O) of dissolved oxygen (DO) under circum-neutral conditions stems from experiments carried out under controlled laboratory conditions. These showed that iron oxidation leads to an increase in δ18ODO values. Here we present the first study on effects of elevated Fe(II) concentrations on the δ18ODO in a natural, iron-rich, circum-neutral watercourse. Our results show that iron oxidation was the major factor for rising dissolved oxygen isotope compositions in the first 85 m of the system in the cold season (February) and for the first 15 m during the warm season (May). Further along the course of the stream, the δ18ODO decreased towards values known for atmospheric equilibration around +24.6 ‰ during both seasons. Possible drivers for these changes may be reduced iron oxidation, increased atmospheric exchange and DO production by oxygenic phototrophic algae mats. In the cold season, the δ18ODO values stabilized around atmospheric equilibrium, whereas in the warm season stronger influences by oxygenic photosynthesis caused values down to +21.8 ‰. In the warm season from 145 m downstream of the spring, the δ18ODO increased again until it reached atmospheric equilibrium. This trend can be explained by respiratory consumption of DO combined with a relative decrease in photosynthetic activity and increasing atmospheric influences. Our study shows that dissolved Fe(II) can exert strong effects on the δ18ODO of a natural circum-neutral spring system even under constant supply of atmospheric O2. However, in the presence of active photosynthesis, with supply of O2 to the system, direct effects of Fe oxidation on the δ18ODO value become masked. Nonetheless, critical Fe(II) concentrations may indirectly control DO budgets by enhancing photosynthesis, particularly if cyanobacteria are involved

Field-level simulation-based inference with galaxy catalogs: the impact of systematic effects

It has been recently shown that a powerful way to constrain cosmological parameters from galaxy redshift surveys is to train graph neural networks to perform field-level likelihood-free inference without imposing cuts on scale. In particular, de Santi et al. (2023) developed models that could accurately infer the value of $\Omega_{\rm m}$ from catalogs that only contain the positions and radial velocities of galaxies that are robust to uncertainties in astrophysics and subgrid models. However, observations are affected by many effects, including 1) masking, 2) uncertainties in peculiar velocities and radial distances, and 3) different galaxy selections. Moreover, observations only allow us to measure redshift, intertwining galaxies' radial positions and velocities. In this paper we train and test our models on galaxy catalogs, created from thousands of state-of-the-art hydrodynamic simulations run with different codes from the CAMELS project, that incorporate these observational effects. We find that, although the presence of these effects degrades the precision and accuracy of the models, and increases the fraction of catalogs where the model breaks down, the fraction of galaxy catalogs where the model performs well is over 90 %, demonstrating the potential of these models to constrain cosmological parameters even when applied to real data.Comment: 29 pages, 11 figures. For the reference in the abstract (de Santi et al. 2023) see arXiv:2302.1410

B cell–adaptive immune profile in emphysema-predominant chronic obstructive pulmonary disease

Cigarette smoke, the major risk factor for COPD in developed countries, causes pulmonary inflammation that persists long after smoking cessation, suggesting self-perpetuating adaptive immune responses similar to those that occur in autoimmune diseases. Increases in the number and size of B cell–rich lymphoid follicles (LFs) have been shown in patients in severe stages of COPD (4), and increased B-cell products (autoantibodies) have been observed in the blood and lungs of patients with COPD (5, 6). Oligoclonal rearrangement of the immunoglobulin genes has been observed in B cells isolated from COPD LFs, suggesting that a specific antigenic stimulation drives B-cell proliferation. Consistently, we have shown that in the COPD lung, there is an overexpression of BAFF (B-cell activation factor of the TNF family), which is a key regulator of B-cell homeostasis in several autoimmune diseases (7) and is involved in the growth of LFs in COPD. However, a network analysis of lung transcriptomics showed that a prominent B-cell molecular signature characterized emphysema preferentially but was absent in AD independently of the degree of airflow limitation (8). In the current study, we investigated the correlation between B-cell responses in lung tissue from patients with COPD and healthy smokers, and the extent of emphysema versus airflow limitation

miR-146a is a pivotal regulator of neutrophil extracellular trap formation promoting thrombosis.

Neutrophil extracellular traps (NETs) induce a procoagulant response linking inflammation and thrombosis. Low levels of miR-146a, a brake of inflammatory response, are involved in higher risk for cardiovascular events, but the mechanisms explaining how miR-146a exerts its function remain largely undefined. The aim of this study was to explore the impact of miR-146a deficiency in NETosis both, in sterile and non-sterile models in vivo, and to inquire into the underlying mechanism. Two models of inflammation were performed: 1) Ldlr-/- mice transplanted with bone marrow from miR-146a-/- or wild type (WT) were fed high-fat diet, generating an atherosclerosis model; and 2) an acute inflammation model was generated by injecting lipopolysaccharide (LPS) (1 mg/Kg) into miR-146a-/- and WT mice. miR-146a deficiency increased NETosis in both models. Accordingly, miR-146a-/- mice showed significant reduced carotid occlusion time and elevated levels of NETs in thrombi following FeCl3-induced thrombosis. Infusion of DNAse I abolished arterial thrombosis in WT and miR-146a-/- mice. Interestingly, miR-146a deficient mice have aged, hyperreactive and pro-inflammatory neutrophils in circulation that are more prone to form NETs independently of the stimulus. Furthermore, we demonstrated that community acquired pneumonia (CAP) patients with reduced miR-146a levels associated with the T variant of the functional rs2431697, presented an increased risk for cardiovascular events due in part to an increased generation of NETs.This work was supported by research grants from Instituto de Salud Carlos III (ISCIII), Fondo Europeo de Desarrollo Regional “Investing in your future” (PI17/00051 y PI17/01421) (PFIS18/0045: A.M. de los Reyes-García) (CD18/00044: S. Águila), and Fundación Séneca (19873/GERM/15). The CNIC is supported by the ISCIII, the Ministerio de Ciencia, Innovación y Universidades (MCIU), and the Fundación Pro CNIC, and is a Severo Ochoa Center of Excellence (SEV-2015-0505). A.B. Arroyo has a research fellowship from Sociedad Española de Trombosis y Hemostasia (SETH). The MCIU supported A.dM. (predoctoral contract BES-2014-067791).S

In vivo CRISPR/Cas9 targeting of fusion oncogenes for selective elimination of cancer cells

This work was supported by CaixaImpulse (CI18-00017;FuGe) to S.R-P. RT-R. is supported by a postdoctoral fellowship from the Asociación Española Contra el Cáncer (AECC). J.C.S. is supported by the Spanish Cell Therapy cooperative research network (TERCEL)(RD16/0011/0011). P.M. also acknowledges the financial support from the Obra Social La Caixa-Fundaciò Josep Carreras. P.M. is an investigator of the Spanish Cell Therapy cooperative research network (TERCEL). A.M.C. acknowledges funding fromXarxa de Bancs de Tumors de Catalunya (XBTC; sponsored by Pla Director d'Oncologia de Catalunya).Fusion oncogenes (FOs) are common in many cancer types and are powerful drivers of tumor development. Because their expression is exclusive to cancer cells and their elimination induces cell apoptosis in FO-driven cancers, FOs are attractive therapeutic targets. However, specifically targeting the resulting chimeric products is challenging. Based on CRISPR/Cas9 technology, here we devise a simple, efficient and non-patient-specific gene-editing strategy through targeting of two introns of the genes involved in the rearrangement, allowing for robust disruption of the FO specifically in cancer cells. As a proof-of-concept of its potential, we demonstrate the efficacy of intron-based targeting of transcription factors or tyrosine kinase FOs in reducing tumor burden/mortality in in vivo models. The FO targeting approach presented here might open new horizons for the selective elimination of cancer cells