Milk whey protein concentration and mRNA associated with β-lactoglobulin phenotype

Two common genetic variants of β-lactoglobulin (β-lg), A and B, exist as co- dominant alleles in dairy cattle (Aschaffenburg, 1968). Numerous studies have shown that cows homozygous for β-lg A have more β-lg and less α-lactalbumin (α-la) and casein in their milk than cows expressing only the B variant of β-lg (Ng-Kwai-Hang et al. 1987; Graml et al. 1989; Hill, 1993; Hill et al. 1995, 1997). These differences have a significant impact on the processing characteristics of the milk. For instance, the moisture-adjusted yield of Cheddar cheese is up to 10% higher using milk from cows of the β-lg BB phenotype compared with milk from cows expressing only the A variant (Hill et al. 1997). All these studies, however, describe compositional differences associated with β-lg phenotype in established lactation only. No information is available on the first few weeks of lactation, when there are marked changes in the concentrations of β-lg and α-la (Pérez et al. 1990)

Cathodoluminescence hyperspectral imaging of trench-like defects in InGaN/GaN quantum well structures

Optoelectronic devices based on the III-nitride system exhibit remarkably good optical efficiencies despite suffering from a large density of defects. In this work we use cathodoluminescence (CL) hyperspectral imaging to study InGaN/GaN multiple quantum well (MQW) structures. Different types of trench defects with varying trench width, namely wide or narrow trenches forming closed loops and open loops, are investigated in the same hyperspectral CL measurement. A strong redshift (90 meV) and intensity increase of the MQW emission is demonstrated for regions enclosed by wide trenches, whereas those within narrower trenches only exhibit a small redshift (10 meV) and a slight reduction of intensity compared with the defect-free surrounding area. Transmission electron microscopy (TEM) showed that some trench defects consist of a raised central area, which is caused by an increase of about 40% in the thickness of the InGaN wells. The causes of the changes in luminescences are also discussed in relation to TEM results identifying the underlying structure of the defect. Understanding these defects and their emission characteristics is important for further enhancement and development of light-emitting diodes

Midwives' role in screening for antenatal depression and postnatal depression

This is an educational paper which aims to inform midwives of tools available to help them make appropriate provisional diagnosis of perinatal depression. A second aim of the paper is to increase midwives' awareness of the relatively newer diagnosis of antenatal depression (AND). Of additional clinical importance, midwives need to recognise that postnatal depression (PND) may be a continuation of AND. To date, screening for AND has received relatively little attention compared with PND, with the evidence-base supporting that the impact can be as severe. It is important for midwives to know that screening for AND can be undertaken using valid and reliable psychometric self-report depression screening questionnaires which have known validity characteristics and threshold cut-off scores. There are several of these tools available to help midwives make the decision about whether or not to refer the women to the mental health team. Current practice in the UK involves the midwife asking an initial short two-item ‘Whooley Question’ screen which, if indicates depression, can be followed up by the women completing a self-report depression screening questionnaire. To highlight their availability, a selection of valid and reliable psychometric self-report depression screening questionnaires are discussed herein, with it being important for midwives to develop a toolkit that can be given to women at clinics, in pamphlets, online or embedded into mobile applications

Psychometric evaluation of the Perinatal Illness Perceptions Scale (PIPS)

Purpose: Perinatal mental health (PMH), is acknowledged as a significant public health issue associated with significant personal, family, social and economic burden. Research demonstrates that healthcare practitioners lack knowledge and confidence in this area but there is likely to be a complexity of factors that may influence practitioner behaviours, including negative attitudes toward people with mental health and inaccurate illness perceptions. This study sought to evaluate the psychometric properties of the Perinatal Illness Perceptions scale (PIPS), a conceptual derivation of the Illness Perception Questionnaire -RevisedDesign: A cross-sectional and exploratory instrument development design, using exploratory factor analysis was employed.Findings: The scale demonstrated good psychometric properties revealing 3 sub-scales; Causes, Consequences (Mother); Consequences (Baby)Originality: Our findings implicate the PIPS as the first robust psychometric measure, which can be used to in the assessment of practitioner knowledge of the causes and consequences of PMH. The PIPS could offer the opportunity to assess these domains within both educational and training context and identify practitioner attitudes which may affect clinical decision making and referral decisions

The CR chondrite clan

The (1) CR chondrites, (2) LEW 85332,(3) Acfer 182,(4) ALH 85085-like chondrites, and (5) Bencubbin-like chondritic breccias are five kinds of chondritic groups which have dramatically different petrographic characteristics, but have mineralogical, bulk chemical, and oxygen and nitrogen isotopic similarities that indicate they are closely related. They are all considered to be members of what we term the CR chondrite clan. Distinguishing characteristics of CR clan chondrites include : (a) reduced, Mg-rich mafic silicates, (b) hydrous matrix and/or dark inclusions (except for Bencubbin-like chondrites), (c) high modal abundances of FeNi metal, (d) FeNi metal having a solar Ni : Co ratio, (e) solar (CI) abundances of refractory and moderately volatile lithophiles, and highly depleted abundances of volatile lithophiles, (f) similar oxygen isotopic compositions of whole rocks, chondrules and matrices, which are on or near the CR mixing line, and (g) anomalously high ^N abundances. CR clan chondrites must have formed in the same local region of the nebula, from closely related reservoirs of materials. The coexistence of anhydrous chondrules with hydrous matrix (and dark inclusions) in the LEW 85332,Acfer 182,and ALH 85085-like chondrites, as well as the widely differing degrees of hydration within and between chondritic samples, implies that hydration of the components was not variable in a single locality, but took place at a variety of locales prior to final lithification of the CR clan chondrites

Coastal ecosystem investigations with LiDAR (light detection and ranging) and bottom reflectance: Lake Superior reef threatened by migrating tailings

Where light penetration is excellent, the combination of LiDAR (Light Detection And Ranging) and passive bottom reflectance (multispectral, hyperspectral) greatly aids environmental studies. Over a century ago, two stamp mills (Mohawk and Wolverine) released 22.7 million metric tons of copper-rich tailings into Grand Traverse Bay (Lake Superior). The tailings are crushed basalt, with low albedo and spectral signatures different from natural bedrock (Jacobsville Sandstone) and bedrock-derived quartz sands. Multiple Lidar (CHARTS and CZMIL) over-flights between 2008–2016—complemented by ground-truth (Ponar sediment sampling, ROV photography) and passive bottom reflectance studies (3-band NAIP; 13-band Sentinal-2 orbital satellite; 48 and 288-band CASI)—clarified shoreline and underwater details of tailings migrations. Underwater, the tailings are moving onto Buffalo Reef, a major breeding site important for commercial and recreational lake trout and lake whitefish production (32% of the commercial catch in Keweenaw Bay, 22% in southern Lake Superior). If nothing is done, LiDAR-assisted hydrodynamic modeling predicts 60% tailings cover of Buffalo Reef within 10 years. Bottom reflectance studies confirmed stamp sand encroachment into cobble beds in shallow (0-5m) water but had difficulties in deeper waters (\u3e8 m). Two substrate end-members (sand particles) showed extensive mixing but were handled by CASI hyperspectral imaging. Bottom reflectance studies suggested 25-35% tailings cover of Buffalo Reef, comparable to estimates from independent counts of mixed sand particles (ca. 35% cover of Buffalo Reef by \u3e20% stamp sand mixtures)

The Third wave in globalization theory

This essay examines a proposition made in the literature that there are three waves in globalization theory—the globalist, skeptical, and postskeptical or transformational waves—and argues that this division requires a new look. The essay is a critique of the third of these waves and its relationship with the second wave. Contributors to the third wave not only defend the idea of globalization from criticism by the skeptics but also try to construct a more complex and qualified theory of globalization than provided by first-wave accounts. The argument made here is that third-wave authors come to conclusions that try to defend globalization yet include qualifications that in practice reaffirm skeptical claims. This feature of the literature has been overlooked in debates and the aim of this essay is to revisit the literature and identify as well as discuss this problem. Such a presentation has political implications. Third wavers propose globalist cosmopolitan democracy when the substance of their arguments does more in practice to bolster the skeptical view of politics based on inequality and conflict, nation-states and regional blocs, and alliances of common interest or ideology rather than cosmopolitan global structures

Bioconductor: open software development for computational biology and bioinformatics.

The Bioconductor project is an initiative for the collaborative creation of extensible software for computational biology and bioinformatics. The goals of the project include: fostering collaborative development and widespread use of innovative software, reducing barriers to entry into interdisciplinary scientific research, and promoting the achievement of remote reproducibility of research results. We describe details of our aims and methods, identify current challenges, compare Bioconductor to other open bioinformatics projects, and provide working examples

Surface modification of a POSS-nanocomposite material to enhance cellular integration of a synthetic bioscaffold

AbstractPolyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU) is a versatile nanocomposite biomaterial with growing applications as a bioscaffold for tissue engineering. Integration of synthetic implants with host tissue can be problematic but could be improved by topographical modifications. We describe optimization of POSS-PCU by dispersion of porogens (sodium bicarbonate (NaHCO3), sodium chloride (NaCl) and sucrose) onto the material surface, with the principle aim of increasing surface porosity, thus providing additional opportunities for improved cellular and vascular ingrowth. We assess the effect of the porogens on the material's mechanical strength, surface chemistry, wettability and cytocompatibilty. Surface porosity was characterized by scanning electron microscopy (SEM). There was no alteration in surface chemistry and wettability and only modest changes in mechanical properties were detected. The size of porogens correlated well with the porosity of the construct produced and larger porogens improved interconnectivity of spaces within constructs. Using primary human bronchial epithelial cells (HBECs) we demonstrate moderate in vitro cytocompatibility for all surface modifications; however, larger pores resulted in cellular aggregation. These cells were able to differentiate on POSS-PCU scaffolds. Implantation of the scaffold in vivo demonstrated that larger pore sizes favor cellular integration and vascular ingrowth. These experiments demonstrate that surface modification with large porogens can improve POSS-PCU nanocomposite scaffold integration and suggest the need to strike a balance between the non-porous surfaces required for epithelial coverage and the porous structure required for integration and vascularization of synthetic scaffolds in future construct design