Evaluation of Annual Companion Crops for the Establishment of Perennial Forage Crops in Eastern Canada

The use of companion crops when establishing perennial forages is desirable as it often reduces weed growth and increases forage biomass in the seeding year. In eastern Canada, oat (Avena sativa L.) is the main species used as companion crop; although other species are used, they have not been systematically evaluated. A field study was established in 2019 at three sites in Québec, Canada, to contrast the use of six annual species as companion crops for the establishment of lucerne (Medicago sativa L.)-timothy (Phleum pratense L.) mixtures. Species evaluated include berseem clover (Trifolium alexandrinum L.), annual ryegrass (Lolium multiflorum Lamarck), forage pea (Pisum sativum L.), forage oat, Japanese millet [Echinochloa esculenta (A. Braun) H. Scholz], and sudangrass [Sorghum × drummondii (Nees ex. Steud.) Millsp. & Chase]. The control treatment consisted of the perennial species seeded without companion crop. Treatments were seeded at three dates (mid-May to early-June, mid-June to early-July, and early August) and evaluated during the seeding year based on biomass production and botanical composition. Overall, across sites, for the first two seeding dates, highest annual forage yields were observed with sudangrass, Japanese millet, and oat as companion crops. The use of these species increased yields by 1.8 to 2.5 Mg ha-1 on a dry matter basis (DM) compared to the control which yielded an average of 3.7 Mg DM ha-1. For the early August seeding, response varied significantly across sites. Annual yields were the highest with the use of oat at two sites (avg. of 2.4 Mg DM ha-1), whereas no differences between treatments were observed at the other site. Companion crop species which maximized total forage yields in the seeding year often reduced weed biomass, but also that of perennial species. The impact of treatments on the survival of perennial forages and their production during the first post-seeding year will be presented in a later publication

(Eta6-arene) ruthenium(II) complexes and metallo-papain hybrid as Lewis acid catalysts of Diels-Alder reaction in water.

International audienceCovalent embedding of a (eta(6)-arene) ruthenium(II) complex into the protein papain gives rise to a metalloenzyme displaying a catalytic efficiency for a Lewis acid-mediated catalysed Diels-Alder reaction enhanced by two orders of magnitude in water

Methodologies to Assess Drug Permeation Through the Blood-Brain Barrier for Pharmaceutical Research

ABSTRACT: The drug discovery process for drugs that target the central nervous system suffers from a very high rate of failure due to the presence of the blood-brain barrier, which limits the entry of xenobiotics into the brain. To minimise drug failure at different stages of the drug development process, new methodologies have been developed to understand the absorption, distribution, metabolism, excretion and toxicity (ADMET) profile of drug candidates at early stages of drug development. Additionally, understanding the permeation of drug candidates is also important, particularly for drugs that target the central nervous system. During the first stages of the drug discovery process, in vitro methods that allow for the determination of permeability using high-throughput screening methods are advantageous. For example, performing the parallel artificial membrane permeability assay followed by cell-based models with interesting hits is a useful technique for identifying potential drugs. In silico models also provide interesting information but must be confirmed by in vitro models. Finally, in vivo models, such as in situ brain perfusion, should be studied to reduce a large number of drug candidates to a few lead compounds. This article reviews the different methodologies used in the drug discovery and drug development processes to determine the permeation of drug candidates through the blood-brain barrie

PRIMA subretinal wireless photovoltaic microchip implantation in non-human primate and feline models

Purpose To evaluate the surgical technique for subretinal implantation of two sizes of PRIMA photovoltaic wireless microchip in two animal models, and refine these surgical procedures for human trials. Methods Cats and Macaca fascicularis primates with healthy retina underwent vitrectomy surgery and were implanted with subretinal wireless photovoltaic microchip at the macula/central retina. The 1.5mm PRIMA chip was initially studied in feline eyes. PRIMA implant (2mm,1.5mm sizes) arrays were studied in primates. Feasibility of subretinal chip implantation was evaluated with a newly-developed surgical technique, with surgical complications and adverse events recorded. Results The 1.5mm implant was placed in the central retina of 11 feline eyes, with implantation duration 43-106 days. The 1.5mm implant was correctly positioned into central macula of 11 primate eyes, with follow-up periods of minimum 6 weeks (n = 11), 2 years (n = 2), and one eye for 3 years. One primate eye underwent multi-chip 1.5mm implantation using two 1.5mm chips. The 2mm implant was delivered to 4 primate eyes. Optical coherence tomography confirmed correct surgical placement of photovoltaic arrays in the subretinal space in all 26 eyes. Intraoperative complications in primate eyes included retinal tear, macular hole, retinal detachment, and vitreous hemorrhage that resolved spontaneously. Postoperatively, there was no case of significant ocular inflammation in the 1.5mm implant group. Conclusions We report subretinal implantation of 1.5mm and 2mm photovoltaic arrays in the central retina of feline and central macula of primate eyes with a low rate of device-related complications. The in vivo PRIMA implantation technique has been developed and refined for use for a 2mm PRIMA implant in ongoing human trials

iNKT cell development is orchestrated by different branches of TGF-β signaling

Invariant natural killer T (iNKT) cells constitute a distinct subset of T lymphocytes exhibiting important immune-regulatory functions. Although various steps of their differentiation have been well characterized, the factors controlling their development remain poorly documented. Here, we show that TGF-β controls the differentiation program of iNKT cells. We demonstrate that TGF-β signaling carefully and specifically orchestrates several steps of iNKT cell development. In vivo, this multifaceted role of TGF-β involves the concerted action of different pathways of TGF-β signaling. Whereas the Tif-1γ branch controls lineage expansion, the Smad4 branch maintains the maturation stage that is initially repressed by a Tif-1γ/Smad4-independent branch. Thus, these three different branches of TGF-β signaling function in concert as complementary effectors, allowing TGF-β to fine tune the iNKT cell differentiation program

Étude sur les stages coopératifs /

Bibliogr.: p. 23-2

Explainable multi-class anomaly detection on functional data

In this paper we describe an approach for anomaly detection and its explainability in multivariate functional data. The anomaly detection procedure consists of transforming the series into a vector of features and using an Isolation forest algorithm. The explainable procedure is based on the computation of the SHAP coefficients and on the use of a supervised decision tree. We apply it on simulated data to measure the performance of our method and on real data coming from industry

VDAC phosphorylation impairment causes mitochondrial membrane permeability during lipid accumulation

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