Calcite moonmilk of microbial origin in the Etruscan Tomba degli Scudi in Tarquinia, Italy

A white deposit covering the walls in the Stanza degli Scudi of the Tomba degli Scudi, Tarquinia, Italy, has been investigated. In this chamber, which is still preserved from any kind of intervention such as cleaning and sanitization, ancient Etruscans painted shields to celebrate the military power of the Velcha family. Scanning electron microscopy analysis has revealed the presence of characteristic nanostructures corresponding to a calcite secondary mineral deposit called moonmilk. Analysis of the microbial community identified Proteobacteria, Acidobacteria and Actinobacteria as the most common phyla in strong association with the moonmilk needle fibre calcite and nanofibers of calcium carbonate. Employing classical microbiological analysis, we isolated from moonmilk a Streptomyces strain able to deposit gypsum and calcium carbonate on plates, supporting the hypothesis of an essential contribution of microorganisms to the formation of moonmilk

Approachability in Stackelberg Stochastic Games with Vector Costs

The notion of approachability was introduced by Blackwell [1] in the context of vector-valued repeated games. The famous Blackwell's approachability theorem prescribes a strategy for approachability, i.e., for `steering' the average cost of a given agent towards a given target set, irrespective of the strategies of the other agents. In this paper, motivated by the multi-objective optimization/decision making problems in dynamically changing environments, we address the approachability problem in Stackelberg stochastic games with vector valued cost functions. We make two main contributions. Firstly, we give a simple and computationally tractable strategy for approachability for Stackelberg stochastic games along the lines of Blackwell's. Secondly, we give a reinforcement learning algorithm for learning the approachable strategy when the transition kernel is unknown. We also recover as a by-product Blackwell's necessary and sufficient condition for approachability for convex sets in this set up and thus a complete characterization. We also give sufficient conditions for non-convex sets.Comment: 18 Pages, Submitted to Dynamic Games and Application

How to make and use a bone \u201cspatula\u201d. An experimental program based on the Mesolithic osseous assemblage of Galgenb\ufchel/Dos de la Forca (Salurn/Salorno, BZ, Italy)

This paper focuses on the techno-functional study of a tool with a smooth end, typologically classifiable as spatula, made from a red deer metatarsal recovered at the early Mesolithic rock-shelter Galgenb\ufchel/Dos de la Forca. The site is located in the middle Adige Valley at Salurn/Salorno (South Tyrol \u2013 Northern Italy) and was dwelled by Sauveterrian hunter-gatherer-fisher-communities from the mid-9th to the mid-8th millennium cal. BC. Subsistence was based on the exploitation of wetland and valley bottom resources including an intense and at times specialized fishing activity. The identification of a probable harpoon fragment among the few but well preserved bone and antler artefacts detected at the site could in fact be part of fishing equipment. The main goal of this study was to recognize the specific use of the tool with a smooth end by means of use-wear analysis. It is quite difficult, indeed, to establish a specific function for this kind of tools as their morphological characters make them suitable for various tasks. A dedicated experimental program was developed to this scope, which involved manufacture and use of spatula replicas for different activities: fish scaling, beading fish heads, working tanned skin, net making, sewing reeds and working birch bark. The implementation of the experimental activity was also aimed at testing tool functionality in the course of the various tasks. The comparison between archaeological and experimental use-wear seems to indicate that the bone tool from Galgenb\ufchel was used as a needle for working vegetal material. On the other hand our experiments revealed a high functionality of this kind of tool also in beading fish heads, working tanned hide and bending birch bark

How to make and use a bone “spatula”. An experimental program based on the Mesolithic osseous assemblage of Galgenbühel/Dos de la Forca (Salurn/Salorno, BZ, Italy)

This paper focuses on the techno-functional study of a tool with a smooth end, typologically classifiable as spatula, made from a red deer metatarsal recovered at the early Mesolithic rock-shelter Galgenbühel/Dos de la Forca. The site is located in the middle Adige Valley at Salurn/Salorno (South Tyrol â Northern Italy) and was dwelled by Sauveterrian hunter-gatherer-fisher-communities from the mid-9th to the mid-8th millennium cal. BC. Subsistence was based on the exploitation of wetland and valley bottom resources including an intense and at times specialized fishing activity. The identification of a probable harpoon fragment among the few but well preserved bone and antler artefacts detected at the site could in fact be part of fishing equipment. The main goal of this study was to recognize the specific use of the tool with a smooth end by means of use-wear analysis. It is quite difficult, indeed, to establish a specific function for this kind of tools as their morphological characters make them suitable for various tasks. A dedicated experimental program was developed to this scope, which involved manufacture and use of spatula replicas for different activities: fish scaling, beading fish heads, working tanned skin, net making, sewing reeds and working birch bark. The implementation of the experimental activity was also aimed at testing tool functionality in the course of the various tasks. The comparison between archaeological and experimental use-wear seems to indicate that the bone tool from Galgenbühel was used as a needle for working vegetal material. On the other hand our experiments revealed a high functionality of this kind of tool also in beading fish heads, working tanned hide and bending birch bark

The Legacy of Mercury Contamination from a Past Leather Manufacturer and Health Risk Assessment in an Urban Area (Pisa Municipality, Italy)

An abandoned open green space in the urban setting of the Municipality of Pisa (Tuscany, Italy) has been designed for renewal to foster the development of recreational activities and improve the lives of the surrounding communities. However, the geochemical site characterization revealed Pb, Cu, Zn and Hg concentrations in the soil exceeding the thresholds imposed by Italian regulations for residential use. Pb, Cu and Zn contents likely reflect the effects of urban vehicle traffic, while Hg contamination represents the legacy of a past artisanal tannery that used Hg(II)-chloride in leather processing in the mid-1900s. Mercury is widely distributed in the area, with the highest concentration in the uppermost soil layer, and reaching about 170 mg/kg in the common dandelion rhizosphere. Chemical extractions and thermal desorption experiments have indicated that most Hg is in the elemental free and matrix-bound fraction, with a possible minor amount (less than 4 wt%) of HgS and negligible methylated forms (0.1 wt%). The data suggest that soil processes could reduce Hg2+ to volatile Hg-0. Mercury in groundwater, hosted in a shallow aquitard in the area, was below 0.2 mu g/L. However, the presence of chloride in groundwater might result in the formation of Hg stable aqueous complexes, increasing Hg release from solids. Future water quality monitoring is hence recommended. The risk assessment highlighted that mercury in soil carries a risk of non-cancerous effects, in particular for children, posing the basis for management planning

Are flood damage models converging to “reality”? Lessons learnt from a blind test

Effective flood risk management requires a realistic estimation of flood losses. However, available flood damage estimates are still characterized by significant levels of uncertainty, questioning the capacity of flood damage models to depict real damages. With a joint effort of eight international research groups, the objective of this study was to compare, in a blind-validation test, the performances of different models for the assessment of the direct flood damage to the residential sector at the building level (i.e. microscale). The test consisted of a common flood case study characterized by high availability of hazard and building data but with undisclosed information on observed losses in the implementation stage of the models. The nine selected models were chosen in order to guarantee a good mastery of the models by the research teams, variety of the modelling approaches, and heterogeneity of the original calibration context in relation to both hazard and vulnerability features. By avoiding possible biases in model implementation, this blind comparison provided more objective insights on the transferability of the models and on the reliability of their estimations, especially regarding the potentials of local and multivariable models. From another perspective, the exercise allowed us to increase awareness of strengths and limits of flood damage modelling, which are summarized in the paper in the form of take-home messages from a modeller's perspective

A comparative analysis of flood damage models: lessons learnt and future challenges

The estimation of flood losses implies the use of vulnerability/exposure models for flood damage and risk assessment (Meyer et al. 2013; Zischg et al. 2018; Wagenaar et al. 2018; Molinari et al. 2019). According to Gerl et al. (2016), in central Europe there are 28 models with 652 functions to assess flood losses, whereas almost half of the functions refer to residential buildings. The main differences among damage models are: (i) spatial scale, (ii) metric (i.e. absolute or relative loss), (iii) exposure assessment (i.e. whole building or affected floors, market values or reconstruction-replacement costs), (iv) number of input variables, (v) calibration/validation with observed losses. These differences pose the issue of model transferability to other urban or environmental contexts. With a joint effort of eight research groups, the objective of this study is to test and compare damage models for residential buildings used or developed by each group, by applying them in a “blind exercise”, i.e. a common flood case study characterised by high availability of hazard and building data, with unknown information on observed losses in the implementation stage of the models. As the research groups use approaches representing many different types and characteristics of models (e.g. univariable – multivariable; absolute – relative; graduated – regression – machine learning), being calibrated based on empirical data stemming from different countries (Austria, France, Germany, Italy, Japan, Netherlands), with different landscapes, the blind exercise provides an extensive comparison of models and their transferability. The analysis of the differences in the ensemble of model outcomes aims at pointing out common patterns or divergent behaviours between model outcomes and with respect to observed losses

Not All Sperm Are Equal: Functional Mitochondria Characterize a Subpopulation of Human Sperm with Better Fertilization Potential

Human sperm samples are very heterogeneous and include a low amount of truly functional gametes. Distinct strategies have been developed to characterize and isolate this specific subpopulation. In this study we have used fluorescence microscopy and fluorescence-activated cell sorting to determine if mitochondrial function, as assessed using mitochondrialsensitive probes, could be employed as a criterion to obtain more functional sperm from a given ejaculate. We first determined that mitochondrial activity correlated with the quality of distinct human samples, from healthy donors to patients with decreased semen quality. Furthermore, using fluorescence-activated cell sorting to separate sperm with active and inactive mitochondria we found that this was also true within samples. Indeed, sperm with active mitochondria defined a more functional subpopulation, which contained more capacitated and acrosome intact cells, sperm with lower chromatin damage, and, crucially, sperm more able to decondense and participate in early development using both chemical induction and injection into mature bovine oocytes. Furthermore, cell sorting using mitochondrial activity produced a more functional sperm subpopulation than classic swim-up, both in terms of improvement in a variety of functional sperm parameters and in statistical significance. In conclusion, whatever the true biological role of sperm mitochondria in fertilization, mitochondrial activity is a clear hallmark of human sperm functionality.Fundação para a Ciência e Tecnologia (POCTI/CVT/49102/2002; SFRH/BD/23571/2005; SFRH/BPD/63120/2009; SFRH/BD/46002/2008; SFRH/BD/46078/2008)Instituto de Investigação Interdisciplinar da Universidade de Coimbra (III/BIO/50/2005