Electrificación de un polígono residencial

Se redacta en el presente proyecto el cálculo y diseño de la red de Baja Tensión, para la alimentación de las distintas cargas que se encuentran distribuidas en el polígono residencial. La red de Baja Tensión incluye todos los elementos que se encuentran a la salida del secundario del transformador, con los fusibles de protección de las líneas y sus respectivas cajas generales de protección según el tipo de abonado o abonados a quienes esté destinado el consumo. Por otra parte también se realizará el cálculo y diseño de la línea de Media Tensión que se deriva de la red de distribución de 20 KV. Disponemos de un punto de acometida (alimentado hipotéticamente desde una subestación transformadora o un entronque aéreo subterráneo), desde el cual se trazará un anillo de media tensión a 20 KV para distribuir la energía eléctrica a los distintos centros de transformación del anillo. Asimismo se trazará una línea en media de tensión para dar servicio, a través de un centro de reparto ubicado en el interior de nuestra urbanización, a un centro de transformación tipo abonado situado a las afueras del recinto objeto del estudio. Además de lo proyectado anteriormente se definirán las características de los Centros de Transformación destinados al suministro de energía eléctrica, así como la justificación y valoración de los materiales empleados en los mismos, se utilizarán dos tipos de Centros de Transformación, los PFU y los MINIBLOK. Para tal fin, se describirá en esta memoria las características esenciales y las condiciones bajo las cuales se realizará la instalación, y que son las correspondientes a la legislación vigente según el Reglamento Electrotécnico de Baja Tensión e Instrucciones Complementarias.Escuela Técnica Superior de Ingeniería IndustrialUniversidad Politécnica de Cartagen

In vitro self-organized mouse small intestinal epithelial monolayer protocol

Developing protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids’ closed geometry has become of great interest during the last few years. Most of the developed culture models showed physiological-relevant cell composition but did not prove self-renewing capacities. Here, we show a simple method to obtain mouse small intestine-derived epithelial monolayers organized into proliferative crypt-like domains, containing stem cells, and differentiated villus-like regions, closely resembling the in vivo cell composition and distribution. In addition, we adapted our model to a tissue culture format compatible with functional studies and prove close to physiological barrier properties of our in vitro epithelial monolayers. Thus, we have set-up a protocol to generate physiologically relevant intestinal epithelial monolayers to be employed in assays where independent access to both luminal and basolateral compartments is needed, such as drug absorption, intracellular trafficking and microbiome-epithelium interaction assays

Luminescent Thermochromism of 2D Coordination Polymers Based on Copper(I) Halides with 4-Hydroxythiophenol

This is the peer-reviewed version of the following article: Troyano, J., Perles, J., Amo‐Ochoa, P., Martínez, J. I., Concepción Gimeno, M., Fernández‐Moreira, V., ... & Delgado, S. (2016). Luminescent Thermochromism of 2D Coordination Polymers Based on Copper (I) Halides with 4‐Hydroxythiophenol. Chemistry–A European Journal, 22(50), 18027-18035., which has been published in final form at https://doi.org/10.1002/chem.201603675. This article may be used for non-commercial purposes in accordance with Wiley-VCH Terms and Conditions for Self-ArchivingSolvothermal reactions between copper(I) halides and 4-mercaptophenol give rise to the formation of three coordination polymers with general formula [Cu3X(HT)2]n(X=Cl, 1; Br, 2; and I, 3). The structures of these coordination polymers have been determined by X-ray diffraction at both room- and low temperature (110 K), showing a general shortening in Cu−S, Cu−X and Cu−Cu bond lengths at low temperatures. 1 and 2 are isostructural, consisting of layers in which the halogen ligands act as μ3-bridges joining two Cu1 and one Cu2 atoms whereas in 3 the iodine ligands is as μ4-mode but the layers are quasi-isostructural with 1 or 2. These compounds show a reversible thermochromic luminescence, with strong orange emission for 1 and 2, but weaker for 3 at room temperature, whereas upon cooling at 77 K 1 and 2 show stronger yellow emission, and 3 displays stronger green emission. DFT calculations have been used to rationalize these observations. These results suggest a high potential for this novel and promising stimuli-responsive materialsThis work was supported by MICINN (MAT2013-46753-C2-1-P). JIM acknowledges funding from the ERC-Synergy Program (Grant ERC-2013-SYG-610256 NANOCOSMOS) and computing resources from CTI-CSIC

Editorial: When the Shape Does Matter: Three-Dimensional In Vitro Models of Epithelial Barriers

The Research Topic presented here includes a collection of selected pre-reviewed manuscripts that describe relevant recent advances in the fields of bioengineering and biophysics with a focus on the generation and analysis of novel three-dimensional (3D) in vitro models of epithelial barriers

Spatio-temporal analysis of geomorphic recovery along an altered ephemeral stream using automated image processing

Ephemeral rivers in the Mediterranean region have been exposed to significant human disturbance over the last century. Recently, there has been a growing interest in restoring their morpho-sedimentary condition, backed by the European Water Framework Directive. Previous research has highlighted the severe geomorphic impacts of sediment extraction, such as reduced channel width and riverbed incision, but the recovery of these channels during annual flows remains poorly understood. This study presents a methodological framework to investigate geomorphologic recovery (signs of landform change) and constraints in the Rambla de Cervera (eastern Spain) from 2018 to 2021. Aerial imagery, LiDAR topography, and field surveys were used for stream segmentation, fluvial landform classification, temporal landform change, and comparison with flow characteristics from a 2D hydraulic model. The study identified three groups of segments with different channel types and recovery mechanisms. Upstream (G1), unconfined valley settings showed minor changes, indicating morphological stability. Middle segments in confined valleys (G2) showed greater alluvial channel recovery and vegetation encroachment in response to flood/no-flood periods. Downstream segments (G3), incised on cemented gravel, were highly responsive to floods but lacked landform stability due to high energy conditions. The findings suggest that channel recovery during annual floods is slow and occurs mainly downstream in degraded areas (G3). Increased sediment flux from upstream and additional sediment storage in new forms are required to recover these channels. This analysis underscores the challenge of interpreting short-term morpho-dynamic changes for long-term river recovery, but provides insight into the morpho-sedimentary conditions required to accelerate recovery of ephemeral streams

Evaluation of zebrafish (Danio rerio) PGCs viability and DNA damage using different cryopreservation protocols

P. 122-130.e2eCryopreservation of primordial germ cells (PGCs) is a better alternative for the conservation of the diploid genome in fish until embryo cryopreservation is achieved. A good cryopreservation protocol must guarantee high survival rates but also absence of genetic damage. In this study, a cell toxicity test using several internal and external cryoprotectants was carried out. The best combination of cryoprotectants (DMSO 5 mol/L, ethylene glicol (EG) 1 mol/L, polyvinyl pyrrolidone (PVP) 4%) was used with and without antifreeze proteins (AFPs) at two different concentrations (10 mg/mL and 20 mg/mL) for cryopreservation trials. Different cryopreservation methods were used with single PGCs, genital ridges, and whole zebrafish embryos using cryovials, 0.5 mL straws, microcapsules, and microdrops. All embryos were obtained from the vasa EGFP zf45 transgenic line and viability was evaluated using trypan blue. High cell viability rates after cryopreservation in 0.5 mL straws were obtained (around 90%) and a decrease in viability was only observed when cells were cryopreserved in microcapsules and when AFP at 20 mg/mL was added to the freezing media. Genetic damage was determined by comet assay and was compared in cells cryopreserved in 0.5 mL straws and microcapsules (lowest viability rate). There were significantly more DNA strand breaks after cryopreservation in the cells cryopreserved without cryoprotectants and in those cryopreserved in microcapsules. Genetic damage in the cells cryopreserved with cryoprotectants in 0.5 mL straws was similar to fresh control samples, regardless of the concentration of AFP used. The decrease in PGC viability with the addition of AFP 20 mg/mL did not correlate with an increase in DNA damage. This study reported a successful method for zebrafish PGC cryopreservation that not only guarantees high cell survival but also the absence of DNA damage

Probiotics reduce anxiety-related behavior in zebrafish

[EN] There is increasing evidence that gut microbiome could have effects on neurological processes and on behavior. In this study we used the novel tank test (NTT) to analyze zebrafish exploring behavior after four months’ supplementation with probiotics with probed antioxidant and anti-inflammatory properties. Results showed that prolonged ingestion of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347 significantly alters the swimming pattern and mean swimming speed in the zebrafish model. After treatment, zebrafish strongly reduced their bottom-dwelling geotactic behavior when placed in a new tank, which could be correlated to a lower state of anxiety.S

Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality after Only One Spermatogenic Cycle in Zebrafish Model

[EN] Infertility is a medical concern worldwide and could also have economic consequences in farmed animals. Developing an efficient diet supplement with immediate effects on sperm quality is a promising tool for human reproduction and for domesticated animal species. This study aims at elucidating the effect of a short-time probiotic supplementation consisting of a mixture of two probiotic bacteria with proven antioxidant and anti-inflammatory activities on zebrafish sperm quality and male behavior. For this purpose, three homogeneous groups of males in terms of motility (<60%) were established. The control group was fed with a normal standard diet. The other received supplements: One group (vehicle control) was fed with maltodextrin and the other received a probiotic preparation based on a mixture (1:1) of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347. The feeding regime was 21 days corresponding with a single spermatogenesis in zebrafish. The preparation did not modify animal weight, positively affected the number of fluent males, increased sperm concentration, total motility, progressive motility, and fast spermatozoa subpopulations. Moreover, the animals fed with the supplement showed different behavior patterns compared to control groups. Our results suggest a diet-related modulation on the exploration activity indicating a lower stress-like conduct. The studied formulation described here should be considered as advantageous in male reproductive biotechnologySIThis work was supported by project AGL2015 68330-C2-1-R (MINECO/FEDER)The authors acknowledge contract PTA2016-11987-I (MINECO/FEDER), Biopolis S.L. (Valencia, Spain), AQUA-CIBUS International Net 318RT0549 (CYTED), and the staff from Planta de Cultivos El Bocal (IEO

Long Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality and Progeny Survival in the Zebrafish Model

This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization[EN] The aim of the present experiment is to study the effects of oral ingestion of a mixture of two probiotic bacteria on sperm quality and progenies. Three homogeneous groups of juvenile zebrafish were created. Once having reached adulthood (3 months postfertilization; mpf), each group received different feeding regimens: a standard diet (control), a maltodextrin-supplemented diet (vehicle control), or a probiotic-supplemented diet (a mixture (1:1) of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347). The feeding regime lasted 4.5 months. Growth parameters (weight and length) were determined at 3, 5, and 7.5 mpf. Sperm motility was evaluated using computer-assisted sperm analysis at 5 and 7.5 mpf. Progeny survival, hatching rate, and malformation rate were also evaluated. Results showed that probiotic-supplemented diet improved growth parameters compared with the standard diet. The highest percentage of motile spermatozoa was reported in the probiotic-fed group. Concomitantly, the percentage of fast sperm subpopulation was significantly lower in samples derived from control males. Furthermore, there was a significant difference in progeny survival between the probiotic-fed group and the control group at three developmental times (24 hours postfertilization (hpf), 5 days postfertilization (dpf) and 7 dpf). In conclusion, in zebrafish, prolonged ingestion of a mixture of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347 has positive effects on growth, sperm quality, and progeny survivalSIThis work was supported by project AGL2015 68330-C2-1-R (MINECO/FEDER)The authors acknowledge contract PTA2016-11987-I (MINECO/FEDER), Biopolis S.L. (Valencia, Spain), AQUA-CIBUS International Net 318RT0549 (CYTED), and the staff from Planta de Cultivos El Bocal (IEO), in particular, Guadalupe Santos Veiga, Serafín Gago Martínez, Jesús Cociña, Diego Vida

Effect of low sperm quality on progeny: a study on zebrafish as model species

[EN] Nowadays a decrease tendency in human sperm quality has been reported mainly in developed countries. Reproductive technologies have been very valuable in achieving successful pregnancies with low quality sperm samples. However, considering that spermatozoa molecular contribution is increasingly important in recent studies, it is crucial to study whether fertilization with low sperm quality could leave a molecular mark on progeny. This study explores the consequences that fertilization with low sperm quality may have on progeny, using zebrafish as a model. Good and bad breeders were established attending to sperm quality analyses and were individually tracked. Significant differences in fertilization and malformation rates were obtained in progenies between high and low quality sperm samples. Moreover an altered miR profile was found in the progenies of bad zebrafish breeders (upregulation of miR-141 and miR -122 in 24 hpf embryos) and as a consequence, some of their targets involved in male sex development such as dmrt1, suffered downregulation. Our results indicate that fertilizing with high sperm quality samples becomes relevant from a new perspective: to avoid molecular alterations in the progeny that could remain masked and therefore produce unexpected consequences in it.SIThis work was supported by AGL2015 68330-C2-1-R project (MINECO/FEDER), PTA2016-11987-I contract (MINECO/FEDER) and AQUA-CIBUS international net 318RT0549 funded by CYTED (Programa Iberoamericano de Ciencia y Tecnologia para el Desarrollo