Screening, characterization and the novo sequencing of broccoli plasma membrane aquaporins by high resolution mass spectrometry

Comunicaciones a congreso

A proteomic approach to study the low temperature stress induction in bell pepper fruit

Comunicaciones a congreso

Relationship of the shelf life of fruits with the content of water and mineral nutrients

[SPA] La tesis doctoral pretende estudiar los mecanismos fisiológicos y moleculares que influyen en el turgor y la firmeza de los frutos, así como en su vida útil. Pudiendo identificar a nivel agronómico la existencia o no de marcadores moleculares, se adaptará la nutrición de Calcio y Boro en el árbol para incidir en la firmeza de los frutos que, en su caso, repercutirá en la calidad del producto final. Se ensayarán de forma directa en campo diversos tratamientos foliares comprobando su eficacia, lo cual nos permitirá mejorar la calidad de los frutos con el consiguiente incremento de su vida útil. [ENG] The doctoral thesis aims to study the physiological and molecular mechanisms that influence the turgor and firmness of the fruits, as well as their shelf life, identifying at the agronomic level the existence or not of molecular markers, the nutrition of Calcium and Boron will be adapted to the tree nutrition to influence the firmness of the fruits and the quality of the final product. Therefore, different foliar treatments will be directly tested in the field, checking their effectiveness, which it will allow to improve fruit quality and increase their shelf life.Francisco José Quirante Moya ha sido financiado por CEBAS-CSIC en el marco de un contrato CDTI con referencia 090202190226- FÉNIX FRESH

Aproximación Proteómica al estudio de la respuesta diferencial del metabolismo energético al estrés por bajas temeperaturas en tomate y pimiento

Comunicaciones a congreso

Effect of salinity and resistance induction using methyl jasmonate on growth and phytochemical properties of sea fennel

[SPA] El hinojo marino (Crithmum maritimum L.) es una halófita herbácea, aromática y comestible, que se produce naturalmente en las zonas costeras de la cuenca mediterránea. En este estudio, se ha evaluado el efecto de la salinidad y la inducción de resistencia mediante metiljasmonato (MeJa) en el crecimiento y las propiedades fitoquímicas (fenoles y flavonoides totales, capacidad antioxidante) del hinojo marino. Las plantas tratadas con MeJa mostraron valores similares de compuestos fenólicos que las plantas control, mientras que el tratamiento salino disminuyó significativamente su contenido en hojas. Respecto a los flavonoides, la salinidad y el MeJa aumentaron significativamente los valores respecto a los controles. La capacidad antioxidante no se vio afectada por ningún tratamiento. Los resultados muestran que el tratamiento con MeJa alivió los efectos negativos de la salinidad sobre el crecimiento sin afectar a la composición fitoquímica de la planta bajo estrés salino. [ENG] Sea fennel (Crithmum maritimum L.) is an herbaceous aromatic and edible halophyte, naturally occurring in coastal areas of the Mediterranean basin. In this study, the effect of salinity and resistance induction using methyl jasmonate (MeJa) on the growth and phytochemical properties (phenols, total flavonoids and antioxidant capacity) of sea fennel was evaluated. The plants treated by MeJa showed similar values of phenolic compounds than the control plants, while the saline treatment significantly decreased its leaf content. Regarding flavonoids, salinity and MeJa significantly increased the values compared to the controls. The antioxidant capacity was not affected by any treatment. The results show that the treatment with MeJa alleviated the negative effects of salinity on growth without affecting the phytochemical composition of the plant under conditions of saline stress

Análisis comparativo de la atención de pacientes con enfermedad neurológica en el servicio de urgencias hospitalario durante el periodo de confinamiento por COVID-19

El nuevo coronavirus (SARS-CoV-2) fue identificado por primera vez en la ciudad china de Wuhan, en diciembre de 2019. Desde entonces el virus se ha propagado a más de 200 países. El 30 de enero de 2020 el brote fue declarado “Emergencia de salud pública de importancia internacional” por la Organización Mundial de la Salud, y el 11 de marzo elevado a “Pandemia internacional”1. El primer diagnóstico en España se registró el 31 de enero, pero no fue hasta marzo cuando los casos comenzaron a crecer exponencialmente. El 14 de marzo el Gobierno de España declaraba el estado de alarma por el que limitaba la libre circulación de personas, entre otras medidas. En Aragón esta limitación estuvo vigente hasta el 4 de mayo, cuando dio comienzo el proceso de desescalada. A fecha de 16 de junio de 2020 se han confirmado en España 244.328 diagnósticos por test de reacción en cadena de polimerasa (PCR)2. En nuestro hospital, a medida que aumentaba la carga asistencial de enfermedad respiratoria en el servicio de urgencias hospitalarias (SUH), se percibía un descenso del resto de enfermedades, incluida la neurológica y, particularmente, la enfermedad cerebrovascular aguda, como se ha reportado en otros estudios3. A nivel mundial, las enfermedades neurológicas representan la causa principal de años de vida perdidos ajustados por discapacidad y la segunda causa de muerte4. En España, la mortalidad por causa neurológica representa el 19% del total anual. Esta cifra se ha incrementado en los últimos 10 años en un 18, 5%. En el caso de Aragón, las enfermedades neurológicas causan el 20, 6% de las muertes5..

Clinical features and health-related quality of life in adult patients with mucopolysaccharidosis IVA: the Spanish experience

Background: Mucopolysaccharidosis (MPS) IVA or Morquio A syndrome is a progressive and disabling disease characterized by a deficiency of the enzyme N-acetylgalactosamine-6-sulphate sulphatase. Its clinical presentation is very heterogeneous and poorly understood in adults. The aim of this study was to describe the clinical manifestations of MPS IVA in adult patients in Spain and to assess their health-related quality of life (HRQoL). Results: Thirty-three patients from nine reference centres participated in the study. The median age was 32 (interquartile range [IQR]: 20.5–40.5) years. The phenotype was classical in 54.5% of patients, intermediate in 33.3% of patients, and non-classical in 12.1% of patients. The most common clinical manifestation was bone dysplasia, with a median height of 118 (IQR: 106–136) cm. Other frequent clinical manifestations were hearing loss (75.7%), ligamentous laxity (72.7%), odontoid dysplasia (69.7%), limb deformities that required orthopaedic aids (mainly hip dysplasia and genu valgus) (63.6%), and corneal clouding (60.6%). In addition, 36.0% of patients had obstructive sleep apnoea/hypopnoea syndrome and 33.3% needed non-invasive ventilation. Cervical surgery and varisation osteotomy were the most common surgical interventions (36.4% each). Almost 80% of patients had mobility problems and 36.4% used a wheelchair at all times. Furthermore, 87.9% needed help with self-care, 33.3% were fully dependent, and 78.8% had some degree of pain. HRQoL according to the health assessment questionnaire was 1.43 (IQR: 1.03–2.00) in patients with the non-classical phenotype, but 2.5 (IQR: 1.68–3.00) in those with the classical phenotype. Seven patients were initiated on enzyme replacement therapy (ERT), but two of them were lost to follow-up. Lung function improved in four patients and slightly worsened in one patient. The distance achieved in the six-minute walk test increased in the four patients who could perform it. HRQoL was better in patients treated with elosulfase alfa, with a median (IQR) of 1.75 (1.25–2.34) versus 2.25 (1.62–3.00) in patients not treated with ERT. Conclusions: The study provides real-world data on patients with MPS IVA. Limited mobility, difficulties with self-care, dependence, and pain were common, together with poor HRQoL. The severity and heterogeneity of clinical manifestations require the combined efforts of multidisciplinary teams

Sustainable Production in the Food Industry Volume 6: Sustainable Primary Production (6): 128-149 (2021)

22 Páginas.-- 2 FigurasChallenges in the food industry relate not only to demographic pressure and environment protection, but also to new consumer demands and specific health requirements. Multidisciplinary studies on raw materials, waste valorisation, efficient green technologies, new products and biodegradable smart packaging are needed. Real-time process control, quality, safety and authenticity will rely on powerful proces analytical technology, multi-sensors and advanced computing and digitalisation systemsPeer reviewe

Inhibited enzymatic reaction of crosslinked lactate oxidase through a pH-dependent mechanism

Lactate oxidase (LOx), recognized to selectively catalyze the lactate oxidation in complex matrices, has been highlighted as preferable biorecognition element for the development of lactate biosensors. In a previous work, we have demonstrated that LOx crosslinking on a modified screen-printed electrode results in a dual range lactate biosensor, with one of the analysis linear range (4 to 50 mM) compatible with lactate sweat levels. It was advanced that such behavior results from an atypical substrate inhibition process. To understand such inhibition phenomena, this work relies in the study of LOx structure when submitted to increased substrate concentrations. The results found by fluorescence spectroscopy and dynamic light scattering of LOx solutions, evidenced conformational changes of the enzyme, occurring in presence of inhibitory substrate concentrations. Therefore, the inhibition behavior found at the biosensor, is an outcome of LOx structural alterations as result of a pH-dependent mechanism promoted at high substrate concentrations.Spanish Ministry of Science and Innovation (MICINN), Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (FEDER) (TEC20013-40561-P and MUSSEL RTC-2015-4077-2). Hugo Cunha-Silva would like to acknowledge funding from the Spanish Ministry of Economy (BES-2014-068214

Patients’ Opinions about Knowing Their Risk for Depression and What to Do about It. The PredictD-Qualitative Study

[Background] The predictD study developed and validated a risk algorithm for predicting the onset of major depression in primary care. We aimed to explore the opinion of patients about knowing their risk for depression and the values and criteria upon which these opinions are based. [Methods] A maximum variation sample of patients was taken, stratified by city, age, gender, immigrant status, socio-economic status and lifetime depression. The study participants were 52 patients belonging to 13 urban health centres in seven different cities around Spain. Seven Focus Groups (FGs) were given held with primary care patients, one for each of the seven participating cities. [Results] The results showed that patients generally welcomed knowing their risk for depression. Furthermore, in light of available evidence several patients proposed potential changes in their lifestyles to prevent depression. Patients generally preferred to ask their General Practitioners (GPs) for advice, though mental health specialists were also mentioned. They suggested that GPs undertake interventions tailored to each patient, from a “patient-centred” approach, with certain communication skills, and giving advice to help patients cope with the knowledge that they are at risk of becoming depressed. [Conclusions] Patients are pleased to be informed about their risk for depression. We detected certain beliefs, attitudes, values, expectations and behaviour among the patients that were potentially useful for future primary prevention programmes on depression.This work was supported by grants from the Andalusian Council of Health [grant reference: 2008/0195][www.juntadeandalucia.es/fundacionprogres​oysalud]; the Department of Health of the Basque Government [grant reference: 2008/111021][www.osakidetza.euskadi.net]; the Spanish Network of Primary Care Research “redIAPP” (RD06/0018), the “Aragón group” (RD06/0018/0020), the “Sant Joan de Deu group” (RD07/0018/0017), “Bizkaya group” (RD07/0018/0018), “Castilla-León group” (RD07/0018/0027) and the “SAMSERAP group” (RD06/0018/0039 and CTS-587) [www.rediapp.org]